Collimated Emission-Port Adapter

Photometrics XT2™ Datasheet
HIGH PERFORMANCE EMCCD & CCD CAMERAS FOR LIFE SCIENCES
Collimated Emission-Port Adapter
For many imaging applications, it is necessary to utilize devices such
as filter wheels and LCTFs on the emission port of a microscope. The
beam emerging from the microscope emission port, however, is coming
to a focus. Optical devices are often placed in the focusing beam,
thereby producing a series of errors, including:
Correct emission imaging with the XT2!
Filter in focusing beam
Filter in focusing beam
Parfocality
Parfocality problem!
Anytime a flat piece of glass (e.g., an emission/barrier filter) is inserted
Focusing light
in
a microscope
focusing beam, the beam will no longer focus at its original
from
location; instead, the location of the focal plane will be shifted. The
amount of shift will depend on the thickness of the filter as well as the
index of refraction of the glass used to make the filter. This focal-shift
New focal the
planedetector and the eyepiece.
error destroys parfocality between
Original focal plane
However, when collimated light passes through an emission filter, the
focal plane is not shifted.
Bandpass Variation
Parfocality problem!
Filter in focusing beam
Focusing light
from microscope
Parfocality problem!
Focusing light
from microscope
New focal plane
Original focal plane
New focal plane
Original focal plane
Filter in focusing light
Filter in focusing light
It is a well-known fact that the bandpass of a filter varies with the
angle at which light hits it. When a filter is placed in a focusing beam,
Edge light
ray
the
at the edge
hits the filter at a different angle than
λ2 of the beam
Center wavelength
Center
of bandpass
λ1
the lightray
at the center
of the(CWL)
beam.
As a result, there is a variation in
is different at edge
λ2
the center wavelength (CWL)
the filter bandpass for different parts
andof
center!
Edge ray
of
the beam. However, when collimated light passes through a filter,
all rays strike at the same angle, eliminating bandpass shift.
Filter in focusing light
Edge ray
Center ray
Edge ray
EdgeCenter
ray ray
λ2
λ1
λ2
λ2
λ1
λ2
Center wavelength
(CWL) of bandpass
is different at edge
and
center!
Center
wavelength
(CWL) of bandpass
is different at edge
and center!
Edge ray
→ Angle of incidence changes from edge to center.
→ Angle of incidence changes from edge to center.
→ Angle of incidence changes from edge to center.
XT2
The Solution…
Filter in collimated light
No wavelength shift!
All light hits filter
at same angle.
λ1
λ1
λ1
Focusing light
from microscope
Lens systems
in XT2
PM-DS-017 Rev B3 ©2013 Photometrics. All rights reserved.
No focal shift!
Original focal
plane is preserved.
• Two-piece optical adapter creates 1 – 4” of collimated (infinity)
Filter in collimated light
space at emission port of microscope, thus allowing addition of
No wavelength shift!
All light hits filter
other optical devices without sacrificing
performance or
Filter in collimated light
at same angle.
No wavelength shift!
introducing optical aberrations
Focusing light
from microscope
• Provides “extra space” at microscope emission
Focusing light
port for installation
of instrumentation such as filter
from microscope
wheels and liquid-crystal tunable filters (LCTFs)
Lens systems
in XT2
• Flexible enough for use with a variety of optical
Lens systems
devices with C-mounts
in XT2
λ1 light hits filter
All
λ1same angle.
at
λ1
λ1
λ1
λ1
No focal shift!
Original focal
plane is preserved.
No focal shift!
Original focal
plane is preserved.
Photometrics XT2™ Datasheet
Collimated Emission-Port Adapter
XT2 Specifications
Detector options
Attachment thread
Max diagonal dimension1
C-mount (male)
12 mm
Microscope attachment options
C-mount (female)
Device attachment options
C-mount (male and female)
Efficiency
~96%
Wavelength sensitivity
400 to 700 nm
Spatial resolution2
~0.4 microns
Weight
1.2 lbs
Dimensions
2.5” x 8.0”
Operating temperature
-10 to 50˚C
1.40
R1.25
1.40
5.80
4.34
3.67
6.12
2.20
Specifications may change without notice.
1.22
1. without experiencing measurable image distortion
.70
2. assumes 100x, 1.6 NA microscope objective
.00
1 – 4"
Optical devices
(e.g., filter wheel or LCTF)
Anodized aluminum
finish on all parts
C-mount male or female thread:
attaches to device
C-mount male or female thread:
attaches to device
C-mount female thread:
attaches to microscope
C-mount port
C-mount male thread:
attaches to C-mount
detector
Collimated (infinity) space
between two components
of adapter
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PM-DS-017 Rev B3 ©2013 Photometrics. All rights reserved.