STUDENT: .…………………………..………………….…. DATE …………………..…......
Water solution as environment of life
Lab test 1. Simple dilution
Concentrations
Simple
dilution
Final
volume of
solution
Volume of
picric acid
Volume of
water
1:2
10 mL
5 mL
5 mL
1:5
100 mL
1:10
100 mL
1:100
100 mL
%
g/100mL
‰
g/L
Molarity
M
mol/L
Molecular weight of picric acid = 229 g/mole
Lab test 2. Serial dilution
Serial dilutions, final volume of solution = 1 mL
Concentrations
Dilution
Amount of
substance
mg/ml
%
g/100mL
‰
g/L
Molarity
M
mol/L
1:2
1:4
1:8
1:16
Lab test 3. Strip test
3.1 Detection of glucose in urine
Molecular weight of glucose = 180 g/mole
Concentration of
glucose
mg/100 (mg%)
mmol/L
μg/100mL
1
3.2 Measuring the pH of urine
Measure the pH of the sample and check the result with the pH scale.
Calculate the [H+] concentration………………………..……………………………………….
Classify the result as normal or abnormal. Explain your choice.
Lab test 4. Preparation of solutions with a different concentration
Prepare solutions according the table and complete the gaps with suitable concentrations.
The final volume of each solution equal 50 mL, d = 1g/mL = 1 g/cm3
Substance
Chemical
formula
NaCl
MW 58,5g/mole
Glucose C6H12O6
MW 180 g/mole
You have to
weigh
[g]
Concentration
Percent %
g/100 mL
Promil ‰
g/L
Miligram
percent
mg%
mg/100mL
Molarity
M
mol/L
0.9
54
Urea CO(NH2)2
MW 60 g/mole
60
CuSO4 x 5H2O
MW 250 g/mole
NiCl2 x 6H2O
MW 237 g/mole
Milimolarity
mM
mmol/L
0.01
100
2
Perform the following conversions:
0.8 mg = .….….. μg
120 mL = .……..…. μL
200 μL = ……...… mL
0.5 μg = …………… ng
0.5 ng = .…..…. pg
8.0 dL = ……………. L
50 mL = ……..….... L
800 dm3 = …….…. cm3
40 μg = ……… mg
500 mg = ……….…. μg
50 cm3 = ……....... dm3
300 ng = …….……. μg
What is the concentration (M, mM, mg% and ‰) of KCl solution if 10 g are dissolved in water
to a final volume of 200 mL? Molecular weight of potassium chloride = 74,5g/mole

molarity M………………………………………………………………………....

mM…………………………………………………………………………………

mg%..............................................................................................................

‰……………………………………………………………………………….…..
What is the pH of a solution whose hydroxide ion concentrations is 5.0 x 10-8 mole/L?
3