Corning® Gentest™
Fresh Human Hepatocytes
Hepatocytes in plates, sandwich culture, and suspension
Corning offers a full portfolio of fresh hepatocytes in plates, sandwich
culture, and suspension to support drug metabolism and transport
studies. High-quality Corning Gentest fresh hepatocytes maintain
morphology and function for long-term assays, such as enzyme induction, toxicity, metabolism of stable compounds, and drug transporter
assays.
Corning Gentest hepatocytes cultured on Collagen I with a Corning
Matrigel® overlay in a sandwich culture form an in vivo-like hepatobiliary network that is critical for studying hepatic uptake and biliary
efflux transporter activity. Compared to Collagen I surfaces alone, the
Corning Matrigel overlay creates a more physiological-like environment, maintains hepatocyte-specific morphology, and improves basal
and induced CYP450 activities.
Features
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Supports ADME assays, such as metabolism, induction, transport,
and toxicity requiring cultured human plateable hepatocytes
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Supports short-term ADME assays in suspension, such as metabolic
stability and uptake transporter studies
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Saves researchers time with premade, ready-to-use hepatocyte
cultures on Collagen I-coated plates with, or without, Corning
Matrigel overlay
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Enables high throughput screening for discovery and development
of drug candidates
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Corning Collagen I substratum with Corning Matrigel overlay
– I mproves cell health with increased basal and induced CYP450
activity
Corning Matrigel Overlay Promotes a
More In Vivo-like Environment
The Corning Matrigel overlay improves basal activities
for CYP450 activities compared to the conventional
Collagen I coated surface.
Corning Matrigel overlay improves both basal and
induced CYP3A4 activity of human fresh hepatocytes
(Corning fresh hepatocyte lot 323 cultured on Corning
BioCoat Collagen I plates)
–S
upports bile canaliculi formation for efflux drug transport studies
–M
aintains typical hepatocyte morphology longer than a Collagen I
surface
Fresh hepatocytes can be cultured for more than one week, which
makes them ideal for studies requiring longer culture times, such as
induction, efflux transport, and toxicity assays.
Multiple Formats Provide Researchers with Flexibility
Corning’s portfolio of fresh hepatocytes includes flasks and 6, 12,
24, 48, and 96 well plates. These formats are available with Corning
BioCoat™ Collagen I as a substratum with, or without, Corning
Matrigel overlay.
CYP3A4 Basal
CYP3A4 Activity, pmol/min/mg
Fresh Hepatocytes Maintain Hepatic Morphology
and Function
CYP3A4 Activity in Corning Matrigel Overlay Sandwich Culture
CYP3A4 Induced
No Matrigel Overlay
With Matrigel Overlay
Corning® Matrigel® Overlay Maintains Good Hepatocyte Morphology
Corning Matrigel overlay maintains good hepatocyte morphology with 100%
monolayer confluence in culture on Corning BioCoat™ Collagen I plates.
Corning fresh hepatocyte lot 323
on day 5 on Corning BioCoat plates
with Corning Matrigel overlay;
Bright field (100x)
Corning Fresh Hepatocytes in Sandwich Culture Enhance Extensive Bile
Canaliculi Formation
Corning fresh hepatocyte lot 323 forms bile canaliculi (Figure A) on day 5 culture
in a Corning BioCoat Collagen I plate with Corning Matrigel overlay culture. Bile
cana­liculi formation is visualized by MRP2 fluorescent substrate: CDF (5-(and-6)Carboxy-2,7-dichloro-fluorescein).
CDF bile accumulation is inhibited by MRP2 inhibitor MK571 (Figure B).
Figure A. Day 5 CDFDA (5-(and-6)-Carboxy 2,7-dichloro-fluorescein diacetate)
without MRP2 Inhibitor MK571 (100x)
Figure B. Day 5 CDFDA with MRP2
Inhibitor MK571 (100x)
Ready to Use Sandwich Culture Plates Save Time and Resources
Hepatocytes are provided in a ready–to-use, pre-made sandwich (collagen I substratum
with Corning Matrigel overlay) culture format. Upon receipt, the plates require minimal
handling before beginning the assay.
Matrigel
Hepatocyte
Collagen I
Hepatocyte sandwich culture on Corning BioCoat Collagen I plates with Corning Matrigel overlay
Important Ordering Information
HepatoLink® is the Corning immediate response system providing researchers with
instant notification when Corning® Gentest™ hepatocyte products are available. For your
convenience, select a preferred contact method—e-mail, phone, and/or SMS text alerts.
Upon registration, you will be sent donor characterization data when a new lot becomes available.
Register online at www.corning.com/lifesciences/hepatolink.
Place your order directly through Corning’s online system. If you have any questions regarding Corning’s
program or your participation, please contact us at 978.442.2200 or e-mail [email protected].
For more information on Corning Matrigel®, which has been cited in more than 6,000 published papers,
please visit www.corning.com/lifesciences.
Corning Gentest Fresh Human Hepatocytes Ordering Information
Human Fresh Plated Hepatocytes on Corning BioCoat™ Collagen I Plates
Human Fresh Hepatocytes on Corning BioCoat Collagen I Plates with Corning Matrigel Overlay
Cat. No.
Description
Qty.
454480
6 well
1 plate
454481
12 well
1 plate
454482
24 well
1 plate
454483
48 well
1 plate
454484
96 well
1 plate
Human Fresh Hepatocytes in Suspension
Cat. No.
Description
Qty.
454401
One Million Human Fresh Hepatocytes in Suspension
(10 million cells minimum order)
1 million cells/vial
Human Fresh Plated Hepatocytes on Corning BioCoat Collagen I Flasks
Cat. No.
454415
454475
Description
Qty.
3
1 flask
3
1 flask
25 cm 75 cm Related Products
Cat. No.
Description
Format
356237
Corning Matrigel Basement Membrane Matrix Phenol Red Free
10 mL
354236
Corning Collagen I Rat Tail
100 mg
See website
Corning BioCoat Collagen I Plates, Dishes, and Flasks
6, 12, 24, 48, 96 well plates;
60 mm, 100 mm, 150 mm dishes
25 cm2, 75 cm2 flasks
See website
Falcon® Cell Cultureware
6, 12, 24, 48, 96 well plates
See website
Falcon Pipets
1, 2, 5, 10, 25, 50, and 100 mL
See website
Corning Gentest ADME/TOX Chemicals including
Substrates, Inhibitors, and Metabolites
See website
Corning GentestSM Contract Research Services
including Induction and Metabolic
Stability Assays using Hepatocytes
Warranty/Disclaimer: Unless otherwise specified, all products are for research use only.
Not for use in humans. Not intended for use in diagnostic or therapeutic procedures.
Corning Life Sciences makes no claims regarding the performance of these products for
clinical or diagnostic applications.
To place an order in the U.S., contact Customer Service at:
tel: 800.492.1110, fax: 978.442.2476
email: [email protected]
For technical assistance, contact Technical Support at:
tel: 800.492.1110, fax: 978.442.2476
email: [email protected]
Outside the U.S., contact your local distributor or visit www.corning.com/lifesciences
to locate your nearest Corning office. For additional Corning product, technical, or
distributor information, call 978.442.2200.
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Corning acquired the BioCoat™, Falcon®, Gentest™, HepatoLink®, and Matrigel® brands.