PRESERVATION AND STORAGE GUIDELINES (UPDATED 8/2012)
ELAP Analyses Approved for the Department of Microbiology
Coliform, E. coli - Drinking Waters
30 hours
120 mL P
Na2S2O3, 4°C
100 mL
Coliform, E. coli - Wastewaters, Sludge
6 hours
120 mL P
Na2S2O3, 4°C
60 mL
Coliform, E. coli - Soil
N/A
250 mL Glass Jar
none, 4°C
50 g
Heterotrophic Plate Count (HPC)
24 hours
120 mL P
Na2S2O3, 4°C
20 mL
Fecal Streptococcus
6 hours
120 mL P
Na2S2O3, 4°C
60 mL
Yeast & Mold
24 hours
120 mL P
Na2S2O3, 4°C
80 mL
ELAP Analyses Approved for the Department of Organic Chemistry
Analysis
Hold Time
(Liquid/Solid/Extract)
Immediate
Chlorine, Residual (Cl2)-330.5, 4500-Cl G
Capacity/Container
1LP
Preservative
none, 4°C
Minimum Amount
1LP
Total Organic Carbon (TOC) 415.1/5310B
28 Days
3 x 40 mL VOA
2 drops 1:1 H3PO4, pH<2, 4°C
40 mL VOA
418.1, - TRPH, IR DetectorP
28 days
1 L AG, 250 mL G
5 ml 1:1 HCL, pH<2, 4°C
1 L, 25 g
Total Oil & Grease(O&G)- 1664
28 days
1 L AG, 250 mL G
5 ml 1:1 HCL, pH<2, 4°C
1 L, 25 g
504.1 - DBCP,EDB by GC/ECD
28 Days
3 x 40 mL VOA
0.5 ml 4% Na2S2O3, 4°C
2 x 40 mL VOA
505 – Organohalide Pesticides & PCB Screen
7 /NA /14 days
3 x 40 mL VOA
0.5 ml 4% Na2S2O3, 4°C
2 x 40 mL VOA
515.1 - Herbicides by GC/ECD
14 /NA /28 days
1 L AG
2.0 ml 4% Na2S2O3, 4°C
1L
524.2 - VOCs by GC/MS
14 days/NA/NA
3 x 40 mL VOA
2 drops 1:1 HCl, pH<2, 4°C
3 x 40 mL VOA
525.2 - Semivolatile Organics by GC/MS
7 /NA /30 days
1 L AG
2.0 ml 4% NaSO3,HCL, 4°C
1L
531.2 - Carbamates by HPLC
28 days/14 days
40 mL AG VOA
3.6 ml K Citrate / 5 drops 4%
Na2S2O3, 4°C
120 mL/50g
547 - Glyphosate by HPLC
14 days/18 mon. @ 0°C
250 mL AG
0.5 ml 4% Na2S2O3, 4°C
120 mL
548.1 - Endothall by GC/FID
7 /NA /14 days
250 mL AG
0.5 ml 4% Na2S2O3, 4°C
120 mL
549.2 - Diquat and Paraquat by HPLC
7 /NA /21 days
500 mL AP
1 ml 4% Na2S2O3, 4°C
250 mL
624 - Volatile Organics by GC/MS
14 /14 days/ NA
3 x 40 mL VOA
2 drops 1:1 HCl, pH<2, 4°C
40 mL,10 g
625 – Base, Neutral and Acidic Organics
7 /14 /40 days
*1 L AG, 250 mL G
2 ml 4% Na2S2O3, 4°C
1 L, 50 g
632 - Diuron by HPLC
7 /NA /40 days
1 L AG
2 ml 4% Na2S2O3, 4°C
1L
608-Organochlorines Pesticides & PCBs
7 /14 /40 days
*1 L AG, 250 mL G
2 ml 4% Na2S2O3, 4°C
1 L, 50 g
600/4-81-045 - PCBs/Aroclors in Oil
28 days/NA/40 Days
20 mL G
none, 4°C
1 mL
8011 - DBCP & EDB by GC/ECD
28 Days/14/40 Days
3x40mL VOA,250 mL G 0.5 ml 4% Na2S2O3, 4°C or none, 4°C 3-40mL VOA,
100g
8021B/8015B - BTEX, MTBE & Gas
14 days/NA/NA
3x40 mL VOA
2 drops 1:1 HCl, pH<2, 4°C
3-40 mL,10 g
8015B(M):Total Petroleum Hydrocarbons
by FID -Diesel, Motor Oil, Kerosene, Jet Fuel
14/14/40 days
1 L AG, 250 mL G
none, 4°C
1 L, 100 g
8081A - Organochlorine Pesticides
7 /14 /40 days
*1 L AG, 250 mL G
2 ml 4% Na2S2O3, 4°C
1 L, 50 g
8082 - PCBs/Aroclors by GC/ECD
7 /14 /40 days
*1 L AG, 250 mL G
2 ml 4% Na2S2O3, 4°C
1 L, 50 g
8141A - Organophosphates by GC/NPD
7 /14 /40 days
*1 L AG, 250 mL G
2 ml 4% Na2S2O3, 4°C
1 L, 50 g
8151A - Chlorinated Herbicides by GC/ECD
7 /14 /40 days
*1 L AG, 250 mL G
2 ml 4% Na2S2O3, 4°C
1 L, 10 g
8260B - Volatile Organics by GC/MS
14 /14 days/ NA
2-40 mL VOA
2 drops 1:1 HCl, pH<2, 4°C
40 mL,10 g
8270C - Semivolatile Organics by GC/MS
7 /14 /40 days
*1 L AG, 250 mL G
2 ml 4% Na2S2O3, 4°C
1 L, 50 g
Total Organic Lead
14 days
500 mL AG/250 mL G
None, 4°C
500 mL, 50g (no
air space)
524.2 – Trihalomethanes (THM) by GC/MS
14 days
3-40 mL amber VOA
2 drops 4% Na2S2O3, 4°C
3 x 40 mL VOA
524.2 - Total THM Potential
7 days
3-40 mL amber VOA
none, 25°C
3 x 40 mL VOA
552.2- HAAs / Haloacetic Acids
14 days
3-60 mL VOA
5 mg NH4Cl, 4°C
3-60 mL VOA
Phenols by 420.1
7 /14 /40 days
*1 L AG, 250 mL G
2 ml 4% Na2S2O3, 4°C
1 L, 50 g
8310 – PAHs by HPLC
7 /14 /40 days
*1 L AG, 250 mL G
2 ml 4% Na2S2O3, 4°C
1 L, 50 g
7 /14 /40 days
*1 L AG, 250 mL G
2 ml 4% Na2S2O3, 4°C
1 L, 10 g
8021B - Chlorinated Hydrocarbons
* Requires additional 2 L per 10 samples for QC (matrix spike) samples.
Page 1 of 4
Preservative formulations:
1:1 HCL Hydrochloric Acid: Measure a volume of Organic Free DI water into a beaker or dispenser. Add an equal volume of concentrated Hydrochloric Acid
slowly, while stirring.
4% (w/v) Na2S2O3 Sodium Thiosulfate: Dissolve 62.8 g Na2S2O3.5 H2O (or 40 g anhydrous Na2S2O3) in one liter of Organic Free DI water.
ZnAc - Zinc Acetate: Dissolve 220 g Zn(C2H3O2)2 .2 H2O in 870 ml DI water. Mix to make 1 L of solution.
Sources: Title 40, Code of Federal Regulations, Parts 136, 141 and 143 (Oct. 2002); RCRA EPA Manual SW-846, Update III (1996) and Update IV (2001); AWWA Standard Methods for
th
th
th
the Analysis of Wastewater and Drinking Water (18 ,19 and 20 Editions); EPA Analysis Handbook for Drinking Water and Wastewater Treatment, EPA Office of Water (1999);
th
th
California Code of Regulations, Titles 17 and 22; Handbook of Environmental Analysis (4 and 5 Editions), Roy-Keith Smith, Ph.D.
ELAP Analyses Approved for the Department of Inorganic Chemistry
Analyses
Hold Time
Capacity / Container
Metals (Total) by EPA 200.7 / 2008:
Antimony (Sb), Arsenic(As), Barium (Ba),
Beryllium (Be), Boron (B), Cadmium (Cd),
Chromium (Cr), Cobalt (Co), Copper (Cu),
Lead(Pb), Magnesium (Mg), Manganese
(Mn), Molybdenum (Mo), Nickel (Ni),
Potassium (K), Selenium (Se), Silica (Si),
Silver (Ag), Sodium (Na), Thallium(Tl),
Vanadium (V), or Zinc (Zn)
6 months
1 L, P / 250 mL soil jar or
zip loc bag
2 ml HNO3, pH<2 / None
1 L, 50 g
125 mL, P
none, 4°C, Filetered and preserved in
the lab with HNO3
500 mL, 50 g
Dissolved Metals
6 months / 24 hrs
unpreserved
Preservation
Minimum Amount
Mercury (Hg)-7470A, 7471A, 245.1
28 days
125 mL, AG
1 ml HNO3, pH<2
300 mL, 50 g
Alkalinity(HCO3,CO3,OH)-2320B,310.1
14 days
250 mL, P
none, 4°C
100 mL
Ammonia (NH3)-SM4500-NH3-B,350.2
28 days
250 mL, P
1 ml H2SO4, pH<2, 4°C
100 mL
Bicarbonate(HCO3)/Carbonate(CO3)
14 days
250 mL, P
none, 4°C
100 mL, 50 g
Biochemical Oxygen Demand (BOD) 5210B/SM405.1
48 hours
1 L, P
none, 4°C
500 mL
Carbon, Total Organic (TOC) 415.1/5310B
28 days
3 x 40 mL AG (VOA)
2 drops 1:1 H3PO4, pH<2,4°C
40 mL VOA
Carbon, Dissolved Organic (DOC) –
415.1/5310B
28 days
3 x 40 mL AG (VOA)
none, 4°C
40 mL VOA
Chemical Oxygen Demand (COD) HACH8000, 5520D, 410.1
28 days
125 mL, P
0.2 ml H2SO4, pH<2, 4°C
50 mL, 50 g
Chloride (Cl)-300.0, 9253, SM4500-Cl-B
28 days
125 mL, P
none, 4°C
50 mL, 25 g
Color-110.2, SM2120B
24 hours
250 mL, P
none, 4°C
100 mL
Conductivity (EC), Specific Conductance
& Resistivity (Drinking Water)-SM 2510B
24 hours
125 mL, P
none, 4°C
50 mL, 50 g
Cyanide, Total (CN)-9014, SM4500-CN C,
335.2, Distillation and Titration
14 days
250 mL, P
0.5 mL (DW-5) NaOH, pH>12, 4°C
50 mL
Fluoride (F)-300.0,SM4500-F,C
28 days
125 mL, P
None
100 mL, 50 g
Hardness (Ca,Mg)-200.7, SM2340B
6 months
125 mL, P
1 ml HNO3, pH<2
125 mL, 50 g
Chromium VI by 7196A
24 hours / 28 Days
125 mL, P
none / Borate Carbonate Buffer, 4°C
125 mL
Hexavalent Chromium-Cr6+- Low Level –
EPA 218.6
24 hours / 28 Days
125 mL, P
none / Borate Carbonate Buffer, 4°C
125 mL
Hydroxide (OH)-403
14 days
250 mL, G
none, 4°C
100 mL, 50 g
Ignitability-1010
14 days
250 mL, G
none, 4°C
100 mL, 50 g
Loss on Ignition/Ash-ASTM-D2974-87
NA
250 mL, G
none, 4°C
50 g
MBAS (Surfactants)-425.1, SM5540C
48 hours
1 L, P
none, 4°C
300 mL
% Moisture / Solids – ASTM-D2974-87
28 days
250 mL, G
none, 4°C
50 g
Page 2 of 4
Analyses
Hold Time
Capacity / Container
Preservation
Minimum Amount
Nitrate (NO3) + Nitrite (NO2)-300.0
48 hours
125 mL, P
none, 4°C
50 mL, 50 g
Nitrate (NO3) SM4500- NO3-D,E, 300.0
48 Hours
125 mL, P
None, 4°C
50 mL, 50 g
Nitrite (NO2)- SM4500- NO2-B, 300.0
48 hours
125 mL, P
none, 4°C
50 mL, 50 g
Nitrogen, Total Kjeldahl (TKN)-351.2-3
28 days
250 mL, P
1 ml H2SO4, pH<2, 4°C
300 mL, 50 g
Nitrogen, Total Organic (TON): EPA 353.2
Nitrate(NO3) + Nitrite(NO2) as Nitrogen(N)
28 days
250 mL, P
1 ml H2SO4, pH<2, 4°C
300 mL, 50 g
Odor-140.1
24 hours
500 mL, G
none, 4°C
300 mL
Oxygen, Dissolved (DO)-360.2, SM421B
8 hours
300 mL BOD Bottle
Fix on site, store dark
300 mL
Perchlorate (ClO4) by EPA 314.0
28 Days
125 mL, P
None, 4°C
50 mL
pH-150.1, SM 4500-H+-B, 9040, 9045
ASAP (<24 hours)
125 mL, P
none, 4°C
50 mL, 10 g
Phosphate, Ortho-300.0, SM4500- P-C
48 hours
125 mL, P
none, 4°C, FILTER IMMEDIATELY
50 mL, 50 g
Phosphate-Phosphorus/Total P365.2,SM4500-P
28 days
250 mL P
Solids: Settable-SS/SM- 2540F, 160.1-5
½ ml H2SO4, pH<2, 4°C
150 mL, 50 g
48 hours
1 L, P
none, 4°C
1 L, 50 g
Solids: Filterable/TDS-2540c/160.1
7 days
1 L, P
none, 4°C
300 mL, 50 g
Solids: Non-Filter TSS, VSS-2540d/160.2
7 days
1 L, P
none, 4°C
1 L, 50 g
Solids: Total, TS-2540B-G/160.3
7 days
1 L, P
none, 4°C
500 mL, 50 g
Solids: Volatile, TVS/TVDS-2540E/160.4
7 days
1 L, P
none, 4°C
500 mL, 50 g
Specific Conductance (EC)-120.1,2510B
28 days (WW)
125 mL, P
none, 4°C
50 mL, 50 g
24 Hours
125 mL, P
none, 4°C
50 mL, 50 g
Sulfate (SO4)- SM4500- SO4 D,300.0
28 days
125 mL, P
none, 4°C
50 mL, 50 g
Sulfide (H2S) SM4500- S2—F
7 days
1LP
2 ml ZnAc2, 2 mL NaOH,pH>9, 4°C
1 L, 50 g
125 mL, P
none, 4°C
50 mL
Sulfite (SO3)-SM4500- SO32—B,377.1
2—
Turbidity(Nephelometric)-SM2130B,180.1
48 hours
GROUPS
Capacity/PLASTIC
Preservative
# / Capacity / GLASS type
Preservative
Full Title 22
3 x 1 L, P
2 x 1 L, P
1 x 125 mL, P
1 x 250 mL, P
1 x 250 mL,AP
1 x 250 mL, P
none, 4°C
2 ml HNO3
1 ml HNO3
NaOH
1 ml Na2S2O3,
none, 4°C
6 x 1 L AG
1 X 500 mL G
3 x 250 mL AG
1 x 40 mL VOA
3 x 40 mL VOA
6 x 40 mL VOA (no septa)
none, 4°C
none, 4°C
0.5 ml Na2S2O3, 4°C
K-Citrate Mono. + Na2S2O3
2 drops HCl, 4°C
Na2S2O3
General Mineral: pH, Alkalinity,
Hardness, Specific Conductance (EC),
TDS, Surfactants (MBAS), CO3, HCO3,
OH, Cl, SO4 ,Ca, Mg, Na, K, Fe, Cu, Mn
,Zn
1 L, P
125 mL, P
none, 4°C
1 ml HNO3
General Physical - Color, Odor, Turbidity
500 mL none, 4°C
Inorganic Chemical: Ag, Al, As, Ba, Be,
CN, Cd, Cr, F, Hg, Pb, Sb, Ni, NO2 -N,
NO3-N, Se, Tl
1 L, P
125 mL, P
250 mL, P
250 mL, P
none, 4°C
1 ml HNO3
NaOH, 4°C
none, 4°C
Storm Water: pH, TSS, EC, TOC or 1664 O&G
1 L, P (none)
&
40 mL G (2 drops HCl) (TOC)
-OR1 L AG (5 ml HCl) (O&G 413.1)
CAM 17 Metals: (6010/7470,200.7/200.8)
for Ag, As, Ba, Be, Cd, Cr, Co, Cu, Hg,
Pb, Sb, Mo, Ni, Se, Tl, V, Zn
125 mL, P
125 mL, P
none, 4°C
2 ml HNO3
RCRA Federal Toxicity Metals:
(6010/7470) for Ag, As, Ba, Cd, Cr, Hg,
Pb, Se
125 mL, P
125 mL, P
none, 4°C
2 ml HNO3
KEY to Container Abbreviations
P - Plastic, High Density Polyethylene (HDPE)
AP - Amber Plastic, HDPE
G - Glass, Clear
AG - Amber Glass
VOA - Volatile Organic Analysis Vial (no air space)
ICAP Metals: (200.7/200.8): Ag, Al, As,
B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, Hg, K,
Mg, Mn, Mo, Na, Ni, Pb, Sb, SiO2, Se, Si,
Sn, Tl, V, Zn, Hardness
125 mL, P
125 mL, P
none, 4°C
2 ml HNO3
ISWP or Priority Pollutant Metals
125 mL, P
125 mL, P
none, 4°C
2 ml HNO3
Page 3 of 4
KEY to Preservative Abbreviations
HCl - 1:1 Hydrochloric Acid
HNO3 - Nitric Acid, concentrated
MCA - Monochloroacetic Acid Buffer (pH 3)
NaOH - Sodium Hydroxide
Na2S2O3 - 4% Sodium Thiosulfate
Irrigation or Mineral Analyses
1 L, P
1 L, P
none, 4°C
2 ml HNO3
Standard Mineral: pH, Alkalinity,
Hardness, Specific Conductance (EC),
TDS, Surfactants (MBAS), CO3, HCO3,
OH, Cl, NO3, SO4 , P, NH3 –N, Ca, Mg,
Na, K, Fe, B, K, SiO2
1L, P
125 mL, P
250 mL, P
none, 4°C
2 ml HNO3
1 ml H2SO4, 4°C
Lead Copper Rule - Must use first draw
sample after water has been undisturbed
in pipes for 6 hours.
1 L, P
2 ml HNO3
Added in Lab
H2SO4 - Sulfuric Acid, concentrated
Miscellaneous Subcontracted Analyses
Asbestos (water/solid)
Bromide-300.0
None
28 days
1 L /Sealed bag
100 mL, P
4°C/none
None
500 mL, 1g
50 mL, 50 g
Chromium VI by 218.6
24 hours
1 L, P
4°C/none
1L
Dioxin (1613, 8280, 8290)
365/30/30 days
2 x 1 L AG
2 ml 4% Na2S2O3, 4°C
2L
Gross Alpha, Radium, Uranium
6 months
1 L P (each)
2 ml HNO3, 4°C
1L
Phenolics, Total-9065/420
28 days
1 L AG
2 ml H2SO4, pH<2, 4°C
1 L, 50 g
Radon-913.0
4 days
8 Oz French Square Glass
None, 4°C
8 Oz
Page 4 of 4