Source Journal of Immunology
Short Communication
Open Access
Evidence of Fc Receptor Gene In an Invertebrate
Michel Leclerc1* and Nicolas Kresdorn2
1
556 rue Isabelle Romée, Sandillon, France
2
GenXPro, Frankfurt, Germany
*Corresponding author: Michel Leclerc, 556 rue Isabelle Romée, Sandillon, France, Tel: 02 38 41 02 09; E-mail:
[email protected]
Abstract
Ig Kappa genes and complement genes were found in the sea star in the past. Recently, we discovered a "sea star
IgKappa gene". More recently, we found an Ig epsilon Fc receptor gene, a Fab gene, when compared to mouse
genome, in immunized and non-immunized sea stars to HRP.The aim of this paper is to come back on the existence
of a primitive antibody in sea star: a primitive Ig antibody in invertebrates.
Keywords: Invertebrate; Sea star; Fc receptor; Immunoglobulin; Primitive antibody
Received: September 27, 2016 Accepted: November 11, 2016 Published: November 18, 2016
© 2016 Michel Leclerc et al; licensee Source Journals
This is an open access article is properly cited and distributed under the terms and conditions of creative commons
attribution license which permits unrestricted use, distribution and reproduction in any medium.
Introduction
Material and Methods
Sea stars were obtained from the Biology Institute
In 2011, it was striking to discover Kappa genes [1] in
the transcriptome of Asterias rubens, when compared to
mouse genome. Two years later we found complement
genes [2] from C1 to C9. At last, we cloned a gene, with
a SMART kit PCR cDNA synthesis (Clontech) [3] : the"
sea star Igkappa gene" with two Ig sites, which lead to
the synthesis of a primitive antibody: an anti-HRP
antibody, by the use of a E.coli plasmid [4]. To date we
find all these elements in immunized and nonimmunized sea stars to HRP (Horse- radish Peroxydase)
and the emergence of low affinity Immunoglobulin
epsilon Fc receptor, next to Fab gene [5], we describe
(Gothenburgh University). Immunizations, genomic
studies were already described [1]. After ligation of
adapters for Illumina's GSII sequencing system, the
cDNA was sequenced on the Illumina GSII platform
sequencing. 1.100 bp from one side of the approximatey
200 bp fragments sequences were assembled using
Velvet [6].
Results
Low affinity Immunoglobulin epsilon Fc receptor
appear in immunized and non-mmunized sea star
genomes.Result with non-mmunized animals is given:
now.
One contig (Contig10847) could be annotated via
Volume 1│Issue 1│2016
Page 1 of 3
© 2016 Michel Leclerc et al; licensee Source Journals. This is an open access article is properly cited
and distributed under the terms and conditions of creative commons attribution license which permits
unrestricted use, distribution and reproduction in any medium.
BLASTX to Mus musculus "Isoform 3 of Low affinity
GGGTCAGCTCATCTTCTTCAAACAGTACGTGGT
immunoglobulin epsilon Fc receptor"
CAACCACAGTACTGCCTAAACAACGGCCATCTC
from the
Swissprot database (FCER2_MOUSE), with an e-value
TTCCGTGATGAATGCTTCTGGATTCCAGACGAT
of 1.49e-11. On an aligned region of 118 amino acids,
GTAGCACGCTGGGTCGATGCTGAACAGAAGTG
63 positive and 40 identical amino acids were found.
CACTAAATACGAAGGCGCTAGGCTGGTCGCCA
5'TCCATTAGGGCAATGAGTGGGACTGCGCGGC
TCACTGACCAAGAAATTAATGATTTTCTTACCG
TTGGCACAGATCATCCCTTTTCTATCACGACAC
ACCTAATTGACAGGGACGTGTGGATCGGGCTC
CTCGAGTCTTTCCACTTGCCGTTGCTAATCTGTA
CATGATACACACAACGAAAGCGATTGGAAATG
ATGCCACACAGTTATTCTCCAATGATTCGACTC
GTCAAACGATTCCCCCGTCAACTACACGAACTT
CAGACAGCTCAGTTTGCTCTTCTTCGATGAAGT
CATCGAAGAAGAGCAAACTGAGCTGTCTGGAG
TCGTGTAGTTGACGGGGGAATCGTTTGACCATT
TCGAATCATTGGAGAATAACTGTGTGGCATTAC
TCCAATCGCTTTCGTTGTGTGTATCATGGAGCC
AGATTAGCAACGGCAAGTGGAAAGACTCGAGG
CGATCCACACGTCCCTGTCAATTAGGTCGGTAA
TGTCGTGATAGAAAAGGGATGATCTGTGCCAA
GAAAATCATTAATTTCTTGGTCAGTGATGGCGA
GCCGCG3'
CCAGCCTAGCGCCGTCGTATTTAGTGCACTTCT
Discussion and Conclusion
GTTCAGCATCGACCCAGCGTGCTACATCGTCTG
Similar results were obtained with immunized and non-
GAATCCAGAAGCATTCATCACGGAAGAGATGG
immunized sea stars. In mouse, it is well-known that Fc
CCGTTGTTTAGGCAGTACTGTGGTTGACCACGT
receptor binds the antibody to the antigen. In this
ACTGTTTGAAGAAGATGAGCTGACCCAATAAC
interaction, antibody can regulate the immune response
CATCATCATCACGAATGGAATCATTGTGAATTTG
[7] through Fc receptor. In mouse the corresponding
TTTGAGATACGTCCGATACGTCCGTCCGTAGAT
antibody is an IgE. Fab gene was recently discovered
GAAAAAACTGCCGAAGTCTCTCACATAATTCCA
[5]. We have not found IgE gene in sea star genome but
CCAGGCATTGTTGATGCCTTGCTGCTCTATGGTT
exclusively Ig Kappa chains. To date all the elements
GATGCTTGGTGGCAGTCCACGAAAGAATGTGC
which composed an antibody are present in the sea star
AGTTAGGGAAAGTCCAGCTTGTATATCTC3''
Asterias rubens and to be honnest a primitive
We give now result with immunized sea star genome,
invertebrate antibody.
when
Reference
compared
to
mouse
genome:One
contig
(Contig1930|m.5483) could be annotated via BLASTX
to Mus
musculus "Isoform
3
of
Low
immunoglobulin epsilon Fc receptor"
affinity
from the
Swissprot database (FCER2_MOUSE), with an e-value
of 7.98e-12. On an aligned region of 118 amino acids,
64 positive and 40 identical amino acids were found.
5'TATACAAGCTGGACTTTCCCTAACTGCACATT
CTTTCGTGGACTGCCACCAAGCATCAACCATAG
AGCAGCAAGGCATCAACAATGCCTGGTGGAAT
TATGTGAGAGACTTCGGCAGTTTTTTCATCTAC
GGACGGACGTATCGGACGTATCTCGAACAAATT
CACAATGATTCCATTCGTGATGATGATGGTTATT
Volume 1│Issue 1│2016
1) Leclerc M, Dupont S, Ortega-Martinez O, Hernroth
B, Krezdorn N, et al. (2011) Evidence of Kappa genes
in
the
sea-star
Asterias
rubens
(Echinoderma).
Immunol.Lett 138: 197-198.
2) Leclerc M, et al. (2013) Immunol.Lett 151: 68-70.
3) Vincent N, et al. (2014) Meta Gene 2: 320-322.
4) Leclerc M, et al. (2014) SAJ.Biotechnology 1: 104.
5) Leclerc M, et al. (2016) Clinical and Trials 2 (4): 188189.
6) Zerbino DR, et al. (2007) Gen.Res 18: 821-829.
7) Nimmerjahn F, et al. (2008) Nature Reviews
Immunology 8: 34-47.
Page 2 of 3
© 2016 Michel Leclerc et al; licensee Source Journals. This is an open access article is properly cited
and distributed under the terms and conditions of creative commons attribution license which permits
unrestricted use, distribution and reproduction in any medium.
Submit your next manuscript to Source Journals and take full advantage of
 Convenient online submission
 Thorough peer review
 No space constraints or colour figure charges
 Immediate publication on acceptance
 Research which is freely available for redistribution
Submit your manuscript at
Volume 1│Issue 1│2016
http://sourcejournals.com/submit-manuscript/
(or)
mail to [email protected]
Page 3 of 3