1. List the steps of DNA replication in order that they occur and what enzyme or structure is responsible
for making that step occur.
2. Define or describe how each term below assists with DNA replication.
Word practice-Fill in the most logical word to make the sentence correct, or answer the question given:
1) Human chromosomes have hundreds of
, where the DNA is unzipped so replication can
begin.
2) DNA polymerase has a function that enables it to detect errors and correct the
3) Why is DNA replication described as semi conservative?
4) What are the 2 major functions of DNA polymerase?
5) For each of the words below, choose an analogy that best suits it and write the letter in the blank. Then, in the
space below the word, write your own analogy to describe the term.
A) Replication
B) Nucleotide
C) DNA Helicase
D) RNA
Multiple Choice Practice: Choose the best answer for the multiple choice questions below.
6) Before the genetic code could be understood, scientists needed to know that a codon is composed of three
nucleotides. This situation is an example of the
A) fact that codons are the building block of life.
C) way that theories can lead to scientific laws.
B) cumulative nature of scientific evidence.
D) ability of scientists to make hypotheses.
7) What is the goal of DNA replication?
A) To make RNA
B) To ensure that every cell has a complete set of identical DNA
C) To make proteins
D) To complete the cell cycle
8) How does a cell ensure that no errors are introduced during replication?
A) Base-pairing rules do not allow errors to occur
B) The origins of replication correct errors
C) DNA polymerase proofreads and corrects the new DNA
D) DNA helicase proofreads and corrects the new DNA
9) What feature of replication ensures that DNA is copied quickly?
A) Replication occurs in thousands of places at once
B) The DNA molecule is replicated from both ends toward the center
C) DNA replicates during both interphase and mitosis
D) Nucleotides are pre-assembled before replication begins.
10) Get a laptop from the cart and visit this website: You will not be able to open this on your iPad. Therefore record
answers on a separate sheet of paper you can turn in for credit.
http://www.wiley.com/college/pratt/0471393878/student/animations/dna_replication/index.html
Click through the animation and answer the questions below as you go. Please make sure to differentiate your answers
by either using a different color or bolding the answers.
What is DNA replication? Fill in the blank: DNA Replication is __ which means that each new strand is half __ and half
__. This was determined by the scientists __ and __.
The correct answer for review question 1 is __
Section 3 – Describe each of the things in the items used in DNA replication listed below. Then answer the questions
about those items that follow.
Helicase
SSB
Primase
Polymerase
Sliding Clamp
RNase H
Ligase
1. Rather than the term SSB, what term did we use for these proteins?
2. We did not discuss a sliding clamp in class, what do you think would happen without them?
3. What replaces the based that are removed by RNase H?
4. What are the names of the strands that the ligase is connecting?
5. Complete the matching section to move forward.
6. What types of bonds does the helicase break?
7. What is the replication fork?
8. In what direction can DNA polymerase make new strand of DNA? Be very specific in your answer.
9. What is needed prior to DNA polymerase synthesis of a new DNA strand?
10. Compare and contrast the leading and lagging strands. Give 2 ways in which they are similar and 2 ways in which
they are different.
11. What fills in the gaps created by RNase H?
12. Does the ligase require energy? In what form?
Go to this site now:
http://www.dnalc.org/resources/3d/DNAWrapBasic_withFX01.html
1. Why is it necessary for DNA to coil up?
2. The process starts when DNA wraps around what?
3. What is the combined loop of DNA and protein called?
4. The “beads on a string make up what fiber?
5. The chromatin fiber is coiled into what?
6. What is the structure that is the end result of repetitive coiling?
7. When are chromosomes found in cells? What is the called for the rest of the cell cycle when it is not in
chromosomes?
8. You can watch this video as many times as you need to in order to answer all the questions.