Curriculum Vitae for Dr. Guijie Zhu Guijie Zhu, Ph.D.
Postdoctral Research Associate
Department of Chemistry and Biochemistry
Notre Dame, IN 46556
Tel: 574-­631-­1175
E-­mail: [email protected]
Education:
¾ January 2009 Ph.D., Dalian Institute of Chemical Physics (DICP), Chinese Academy of
Sciences. 2002-2009
Dissertation title: Preparation of monolithic immobilized pH gradient materials
and the applications in protein separation
Supervisor: Prof. Yukui Zhang (Member of Chinese Academy of Sciences)
Prof. Lihua Zhang
Research Experience
¾ September 2008-March 2011
Research Assistant, Dalian Institute of Chemical Physics (DICP), Chinese Academy of Sciences.
¾ March 2011-now
Postdoctral Research Associate, Norman Dovichi group, University of Notre Dame
I nstrument Skills
™ Electrophoresis P/ACETM 5010 Capillary electrophoresis instrument (BECKMAN COULTER)
™ P/ACETM MDQ Capillary electrophoresis instrument (BECKMAN COULTER)
™ TriSepTM 2010GV Pressurized Capillary Electrochromatography System
(Unimicro Technology)
®
™ MINI-PROTEAN 3 Cell (Bio-Rad Laboratories, Inc.)
™
™
™
™
™
HPLC LC20AD-SPD-M20A HPLC System (SHIMADZU)
A paradigm GM4 ȝHPLC system (Michrom Bioresources Inc., Auburn, CA)
Ultimate 3000 sensitive nanoflow LC/MS (Dionex Corporation)
MS LCQDUO quadrupole ion trap mass spectrometer (LCQ-IT MS, Thermo Fisher)
LTQXL ion trap mass spectrometers coupled with a quaternary Surveyor
pump(Thermo-Fisher)
™ LTQ-Orbitrap-Velos(Thermo-Fisher)
™ UltrafleX III MALDI TOF/TOF (Bruker-Daltonics)
Professional expertise
Analytical Chemistry, Separation Science, Protein Analysis
1， The preparation of monolithic immobilized pH gradient materials
2,
3,
Preparation of M13 phage library based protein equalizer
CE-MS for protein and peptides analysis (CIEF-ESI-MS/MS, CZE-ESI-MS/MS)
1 Curriculum Vitae for Dr. Guijie Zhu Awards
☆ 2007 Third Rank Award, Excellent Papers on science of Dalian (Macroporous
polyacrylamide-based monolithic column with immobilized pH gradient for protein analysis)
☆ 2006 First Rank Award, The China Association for Instrumental Analysis (CAIA) Awards,
(Multidimensioal capillary electrophoresis for the protein separation)
Publications:
1. Guijie Zhu, Huiming Yuan, Peng Zhao, Lihua Zhang, Zhen Liang, Weibing Zhang, Yukui
Zhang. Macroporous polyacrylamide-based monolithic column with immobilized pH
gradient for protein analysis, Electrophoresis, 2006, 27, 3578-3583
2. Guijie Zhu, Chun Yang, Lihua Zhang, Zhen Liang, Weibing Zhang, Yukui Zhang
Preparation and application of monolithic columns with narrow immobilized pH gradients,
Talanta, 2006, 70, 2-6
3. Guijie Zhu, Lihua Zhang, Zhen Liang, Weibing Zhang, Yukui Zhang, Recent development of
monolithic materials as matrix in microcolumn separation systems, J. Sep. Sci. ，
2007,30,792-803 (I nvited review)
4. Guijie Zhu, Chun Yang, Hechun Liu, Lihua Zhang, Weibing Zhang,Yukui Zhang,
Construction of a new method for mobilizing the zones of CIEF and its application, Chemical
Journal of Chinese Universities, 2005,26,654-656
5. Guijie Zhu, Weibing Zhang, Lihua Zhang, Zhen Liang, Yukui Zhang, Preparation of
polyacrylamide based monolith with immobilized pH gradient and its application for protein
analysis. Science in China Series B: Chemistry, 2007, 50, 526-529
6. Guijie Zhu, Lihua Zhang, Zhen Liang, Weibing Zhang, Yukui Zhang, The development of
monolithic materials and their application on micro separation system, Chinese Journal of
Chromatography, 2007,25,122-128
7. Guijie Zhu, Zhen Liang, Lihua Zhang , Yukui Zhang, Developments and applications of
multidimentional liquid chromatography in proteomics, Chinese Journal of
Chromatography, 2009, 27,518-525
8. Peng Zhao, Guijie Zhu, Lihua Zhang, Zhen Liang, Zonghai Li,Yukui Zhang, New method
for determination of average scFv fragment number displayed on the M13 phage surface,
Pure Appl. Chem., 2010, 82, 205-211
9. Peng Zhao, Guijie Zhu, Weibing Zhang, Lihua Zhang, Zhen Liang, Yukui Zhang, Study of
multiple binding constants of dexamethasone with human serum albumin by capillary
electrophoresis±frontal analysis and multivariate regression, Anal Bioanal Chem, 2009, 393,
257±261
10. Dingyin Tao, Guijie Zhu, Liangliang Sun, Junfeng Ma, Zhen Liang, Weibing Zhang, Lihua
Zhang, Yukui Zhang, Serially coupled microcolumn reversed phase liquid chromatography
2 Curriculum Vitae for Dr. Guijie Zhu for shotgun proteomic analysis, Proteomics, 2009, 9, 2029±2036
11. Chun Yang, Guijie Zhu, Lihua Zhang, Weibing Zhang, Yukui Zhang, Repeatedly usable
immobilized pH gradient in a monolithic capillary column, Electrophoresis, 2004, 25,
1729±1734
12. Bin Han, PingliWang, Guijie Zhu, Lihua Zhang, Feng Qu, Yulin Deng, Yukui Zhang,
Microchip free flow isoelectric focusing for protein prefractionation using monolith with
immobilized pH gradient, J. Sep. Sci., 2009, 32, 1211-1215
13. Huiming Yuan, Lihua Zhang, Chunyan Hou, Guijie Zhu, Dingyin Tao, Zhen Liang, Yukui
Zhang, Integrated Platform for Proteome Analysis with Combination of Protein and Peptide
Separation via Online Digestion, Anal. Chem., 2009, 81, 8708±8714
14. Liangliang Sun, Junfeng Ma, Xiaoqiang Qiao, Yu Liang, Guijie Zhu, Yichu Shan, Zhen
Liang, Lihua Zhang, Yukui Zhang, Integrated Device for Online Sample BufferExchange,
Protein Enrichment, and Digestion, Anal. Chem., 2010, 82, 2574±2579
15. Hechun Liu, Lihua Zhang, Guijie Zhu, Weibing Zhang, Yukui Zhang,An Etched Porous
Interface for On-Line Capillary Electrophoresis-Based Two-Dimensional Separation System,
Anal. Chem., 2004, 76, 6506-6512
Patents:
1. Yukui Zhang, Guijie Zhu, Chun Yang, Hechun Liu, Lihua Zhang, Weibing Zhang, A novel
mobilization method for capillary isoelectric focusing, Appl. No. 200510046453.9
2. Yukui Zhang, Hechun Liu, Guijie Zhu, Lihua Zhang, Weibing Zhang, An equipment for
2D-CE, Appl. No. CN200410062794.0
References:
Professor Norman Dovichi
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 465565670 USA
Phone: 1-574-631-2778
Email: [email protected]
Professor Yuikui Zhang
Adress: Group 1810, National Chromatographic R. & A. Center, Dalian Institute of
Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian,
China 116023
Phone number: +86-411-84379560
E-mail: [email protected]
Professor Lihua Zhang
Adress: Group 1810, National Chromatographic R. & A. Center, Dalian Institute of
Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian,
3 Curriculum Vitae for Dr. Guijie Zhu China 116023
Phone number: +86-411-84379720
E-mail: [email protected]
Professor Weibing Zhang
Adress: East China University of Science and Technology
Shanghai, China Meilong Road 130, 200237
Phone number: +86-21-64252145
E-mail: [email protected]
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