Ch 11 Cell cycle and division Practical 11.1 Examination of different stages of the cell cycle Questions (p. 11-2) 1 Cell Mitosis (Nuclear division) Cell growth (Prophase) Stage 1 Stage 2 (Metaphase) Cytoplasmic Stage 4 division (Anaphase) (Telophase) ✓ A B ✓ C ✓ D Stage 3 ✓ E ✓ F ✓ ✓ G ✓ H ✓ I ✓ J 2 Interphase: Individual chromosomes cannot be distinguished. Prophase: Chromosomes become visible, each is seen to consist of two chromatids held together at the centromere. The nuclear membrane disintegrates. Metaphase: The chromosomes line up in the middle of the cell. Anaphase: The two chromatids of each chromosome separate and move to the opposite poles of the cell. The cytoplasm starts to divide. Telophase: New nuclear membranes form around each set of chromosomes. The chromosomes uncoil to become chromatin again. 3 The cells near the tips divide actively by mitotic cell division. Practical 11.2 Investigation of the relative time required for each stage of the cell cycle Results (p. 11-6) 1 2 Number of cells in the stage Area 1 (A1) Area 2 (A2) Area 3 (A3) Relative time spent in the stage (min) Interphase 23 25 24 551 Prophase 3 2 3 61 Metaphase 2 2 1 38 Anaphase 1 1 1 23 Telophase 1 2 1 30 Cytoplasmic division 1 0 1 15 Stage Total number of cells in each area T1 = 31 T2 = 32 T3 = 31 3 Questions (p. 11-8) 1 To expose more cells for observation. 2 a To intensify the stain. b To avoid the slide being overheated and broken. 3 The number of cells in each phase is directly proportional to the time required by the cells in that phase. 4 a (Answer varies with results.) b (Answer varies with results.) a The results are different. This is because different students and groups used slightly different criteria to categorize the phases (i.e. personal judgment and subjectivity) or different groups collected their data from different samples. b No. The findings are based on an assumption without testing. If the assumption is wrong, the findings will also be incorrect. c Subjectivity; Tentativeness; Observation vs. Inference; Creativity; Empirically based. 5 Conclusion (p. 11-9) Interphase takes the longest time in the cell cycle. Phases in mitosis are relatively short. Practical 11.3 Examination of meiotic cell division Questions (p. 11-13) 1 4. 2 8. 3 abdce a) Homologous chromosomes line up at the middle of the cell. → b) Separation of homologous chromosomes. → d) Formation of two daughter cells each with chromosome number halved. → c) Arrangement of chromosomes at the middle of the cell. → e) Formation of four haploid daughter cells.
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