Isoschizomers
TECHNICAL REFERENCE
Isoschizomers. The enzymes in boldface type are available from Promega.
AatI
AatII
AccI AccIII Acc65I
AccB1I
AccB7I
AclNI
AclWI
AcyI AcsI
AfaI
AfeI
AflII
AgeI
AhaIII AhdI
AluI AlwI Alw26I1 Alw44I AocI ApaI
ApaLI ApoI
AseI AsnI AspI AspEI
Asp700I
Asp718I AsuI AsuII AsuHPI
AvaI AvaII AxyI
BalI
BamHI BanI
BanII BbeI
Bbr PI
BbsI1
BbuI
BclI
BcnI
BfrI
BglI
BglII
BmyI BpmI
BsaHI
BsaMI
BsaOI
BseAI
BseNI
BsePI
Bsh1285I
BshNI
Bsh1365I
BsiEI
StuI, Eco147I, Pme55I, SseBI
—
FblI, XmiI
BspEI, MroI
Asp718I
KpnI*
BanI, BshNI, Eco64I
PflMI, Van91I
Spel
AlwI
BbiII, Hin1I, Hsp92I,
BsaHI, Msp171I
ApoI
RsaI
Csp6I
Eco47III
Bst 98I
PinAI
DraI
EclHKI
—
AclWI
BsmAI
ApaLI
Bsu36I, CvnI
—
Bsp120I* Alw44I, VneI
AcsI
VspI, AsnI
VspI, AseI
Tth111I
AhdI, Eam1105I, EclHKI
XmnI
Acc65I
KpnI*
Sau96I, Cfr13I
Csp45I, BstBI
HphI
Ama87I, BcoI, BsoBI, Eco88I
SinI, Eco47I, HgiEI
Bsu36I
MscI, MluNI
—
AccBI, BshNI, Eco64I
Eco24I
—
NarI*
Eco72I, PmlI
Bsc91I, BpiI
PaeI, SphI
BsiQI, FbaI
NciI
Bst98I
—
—
Bsp1286I
GsuI
Hsp92I
BsmI
Bsh1285I, BsiEI
AccIII
BsrSI, BsrI
BssHII, PauI
BsaOI
BanI, AccB1I, Eco64I
BsrBRI
BsaOI
AGGtCCT
GACGTtC
GTt(A/C)(G/T)AC
TtCCGGA
GtGTACC
GGTACtC
GtG(C/T)(G/A)CC
CCAN4tNTGG
AtCTAGT
GGATCNNNNt
G(A/G)tCG(T/C)C
(G/A)tAATT(C/T)
GTtAC
GtTAC
AGCtGCT
CtTTAAG
AtCCGGT
TTTtAAA
GACNNNtNNGTC
AGtCT
GGATCNNNNt
GTCTC(1/5)
GtTGCAC
CCtTNAGG
GGGCCtC
GtGGCCC
GtTGCAC
(G/A)tAATT(C/T)
ATtTAAT
ATtTAAT
GACNtNNGTC
GACNNN/NNGTC
GAANNtNNTTC
GtGTACC
GGTACtC
GtGNCC
TTtCGAA
GGTGAN8t
Ct(C/T)CG(G/A)G
GtG(A/T)CC
CCtTNAGG
TGGtCCA
GtGATCC
GtG(T/C)(A/G)CC
G(A/G)GC(T/C)tC
GGCGCtC
GGtCGCC
CACtGTG
GAAGAC(2/6)
GCATGtC
TtGATCA
CCt(C/G)GG
CtTTAAG
GCCNNNNtNGGC
AtGATCT
G(G/A/T)GC(C/A/T)tC
CTGGAG(16/14)
G(A/G)tCG(T/C)C
GAATGC(1/–1)
CG(A/G)(T/C)tC
TtCCGGA
ACTGGN (1/–1)
GtCGCGC
CG(A/G)(T/C)tCG
GtG(T/C)(A/G)CC
GATNNtNNATC
CG(A/G)(T/C)tCG
BsmI
Bsm AI1
BsoBI
Bsp19I
Bsp68I
Bsp106I
Bsp119I
Bsp120I
Bsp143I
Bsp143II
Bsp1286I BspCI
BspDI
BspEI
BsrI1
BsrSI1
BssHII
Bst98I
BstBI
BstEII
BstNI
BstOI
BstXI
BstYI BstZI
Bsu15I
Bsu36I
BsuRI
CfoI Cfr9I
Cfr13I
Cfr42I
ClaI CpoI
CspI Csp6I
Csp45I CvnI DdeI
DpnI2
DpnII
DraI EagI Eam1105I
Ecl136II
EclHKI
EclXI
Eco24I
Eco32I
Eco47I Eco47III
Eco52I Eco64I
Eco81I Eco88I
Eco91I
Eco105I BsaMI
Alw26I
AvaI, Ama87I, BcoI, Eco88I
NcoI
NruI
ClaI, BspDI
Csp45I, NspV, BstBI
—
ApaI
MboI, Sau3AI, NdeII
HaeII
BmyI, SduI
PvuI
ClaI
AccIII
BsrSI, BseNI
BseNI, BsrI
BsePI, PauI
AflII, BfrI
Csp45I, NspV, Bsp119I
BstPI, Eco91I, PspEI
BstOI, MvaI EcoRII*
BstNI, MvaI
EcoRII*
–
XhoII, MflI
Eco52I, EagI, XmaIII, EclXI
ClaI
CvnI, AocI, Eco81I
HaeIII, PalI
HhaI
Hin6I, HinP1I
XmaI
SmaI*
Sau96I SacII BanIII, Bsp106I, BspDI, Bsu15I
CspI, RsrII
CpoI, RsrII
—
RsaI*, AfaI*
BstBI, NspV, Bsp119I
Bsu36I
BstDEI
—
DpnII*
MboI, Sau3AI, NdeII, DpnI*
—
Eco52I, Bst ZI, Ecl XI, XmaIII
EclHKI, AhdI, AspEI
EcoICRI
SacI*
AhdI, Eam1105I, AspEI
BstZI, EagI, Eco52I, XmaIII
BanII, FriOI
EcoRV
AvaII, SinI
AfeI
BstZI, XmaIII, EagI, EclXI
BanI, BshNI, Eco64I
Bsu36I
AvaI
BstEII
SnaBI
GAATGCNt
GTCTC(1/5)
C(C/T)CG(G/A)G
CtCATGG
TCGtCGA
ATtCGAT
TTtCGAA
GtGGCCC
GGGCCtC
t
GATC
(A/G)GCGCt(T/C)
G(G/A/T)GC(C/A/T)tC
CGATtCG
ATtCGAT
TtCCGGA
ACTGGN(1/-1)
ACTGGN(1/-1)
GtCGCGC
CtTTAAG
TTtCGAA
GtGTNACC
CCt(A/T)GG
t
CC(A/T)GG
CCt(A/T)GG
t
CC(A/T)GG
CCANNNNNtNTGG
(A/G)tGATC(T/C)
CtGGCCG
ATtCGAT
CCtTNAGG
GGtCC
GCGtC
GtCGC
CtCCGGG
CCCtGGG
GtGNCC
CCGCtGG
ATtCGAT
CGtG(A/T)CCG
CGtG(A/T)CCG
GtTAC
GTtAC
TTtCGAA
CCtTNAGG
CtTNAG
GmeAtTC
GAtTC
t
GATC
GAtTC
TTTtAAA
CtGGCCG
GACNNNtNNGTC
GAGtCTC
GAGCTtC
GACNNNtNNGTC
CtGGCCG
G(AG)GC(TC)tC
GATtATC
GtG(A/T)CC
AGCtGCT
CtGGCCG
GtG(TC)(AG)CC
CCtTNAGG
Ct(TC)CG(AG)G
GtGTNACC
TACtCTA
Isoschizomers
Isoschizomers (continued).
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PART #GE633
TECHNICAL REFERENCE
www.promega.com
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The enzymes in boldface type are available from Promega.
Enzyme Isoschizomer(s)
Eco130I
Eco147I
EcoICRI
EcoRI
EcoRII
EcoRV
EcoT14I
EcoT22I
EheI
FokI2
HaeII
HaeIII HapII HgiEI
HhaI Hin1I HincII HindII HindIII HinfI
HinP1I HpaI
HpaII3 Hsp92I Hsp92II I-PpoI
KasI
KpnI KspI
MboI MboII1
MflI MluI
MluNI MroI
MscI MseI
MspI3 MspA1I
MstII
MvaI
NaeI NarI
NciI
NcoI
NdeI
NdeII
NgoMIV NheI NlaIII
NotI NruI NsiI NspV
StyI
StuI
Ecl136II
SacI*, SstI*
—
—
BstOI*, BstNI*, MvaI*
Eco32I
StyI
NsiI
—
NarI*
—
Bsp143II
BsuRI, PalI
HpaII, MspI
Eco47I, SinI, AvaII
CfoI
HinP1I*, Hin6I*
AcyI, Hsp92I
HindII
HincII
—
—
—
HhaI*, CfoI*
KspAI
MspI, HapII
AcyI, BsaHI, Hin1I
NlaIII
­—
—
NarI*
­— Acc65I*, Asp718I*
SacII
Sau3AI, NdeII, DpnII
—
XhoII
—
BalI, MscI
AccIII
BalI, MluNI
Tru9I
HpaII, HapII
NspBII
Bsu36I
BstOI, BstNI
EcoRII* —
NgoMIV
—
EheI*
KasI*
BbeI*
BcnI
Bsp19I
—
MboI, Sau3AI, DpnII
—
NaeI*
—
Hsp92II
—
Bsp68 I
EcoT22I, Mph1103I
Csp45I, BstBI, Bsp119I
Recognition Sequence
Enzyme Isoschizomer(s)
CtC(A/T)(T/A)GG
AGGtCCT
GAGtCTC
GAGCTtC
GtAATTC
t
CC(A/T)GG
CCt(A/T)GG
GATtATC
CtC(A/T)(A/T)GG
ATGCAtT
GGCtGCC
GGtCGCC
GGATG(9/13)
(A/G)GCGCt(T/C)
GGtCC
CtCGG
GtG(A/T)CC
GCGtC
GtCGC
G(A/G)tCG(T/C)C
GT(T/C)t(A/G)AC
GT(T/C)t(A/G)AC
AtAGCTT
GtANTC
GtCGC
GCGtC
GTTtAAC
CtCGG
G(A/G)tCG(C/T)C
CATGt
CTCTCTTAAtGGTAGC
GtGCGCC
GGtCGCC
GGTACtC
GtGTACC
CCGCtGG
t
GATC
GAAGA(8/7)
(A/G)tGATC(T/C)
AtCGCGT
TGGtCCA
TtCCGGA
TGGtCCA
TtTAA
CtCGG
C(A/C)GtC(G/T)G
CCtTNAGG
CCt(A/T)GG
t
CC(A/T)GG
GCCtGGC
GtCCGGC
GGtCGCC
GGCtGCC
GtGCGCC
GGCGCtC
CCt(C/G)GG
CtCATGG
CAtTATG
t
GATC
GtCCGGC
GCCtGGC
GtCTAGC
CATGt
GCtGGCCGC
TCGtCGA
ATGCAtT
TTtCGAA
NspBII
PaeI PaeR7I PalI PflMI
PinAI
PstI
PvuI PvuII
RsaI RsrII
SacI SacII SalI
Sau3AI Sau96I ScaI
SduI
SfiI
SfuI
SgfI
SinI SmaI
SnaBI SpeI
SphI SspI SstI SstII StuI StyI TaqI Tru9I
Tth111I
TthHB8I
Van91I
VneI
VspI XbaI XhoI XhoII
XmaI XmaIII XmaCI
XmnI MspA1I
BbuI, SphI
XhoI
HaeIII, BsuRI
AccB7I, Van91I
AgeI
—
BspCI
—
AfaI
CspI, CpoI
SstI
Ecl136II*, EcoICRI*
SstII, KspI, Cfr42I
—
MboI, NdeII, DpnII
Cfr13I
—
Bsp1286I
—
Csp45I
—
AvaII, Eco47I
—
XmaI*, Cfr9I*
Eco105I AclNI
BbuI, PaeI
—
SacI
EcoICRI*
SacII
AatI, Eco147I
EcoT14I
TthHB8I
MseI
AspI
TaqI
AccB7I, PflMI
ApaLI, Alw44I AseI, AsnI
—
PaeR7I
BstYI, MflI
Cfr9I, XmaCI,
SmaI*
Eco52I, BstZI, EagI, Ecl XI XmaI
SmaI*
Asp700I
Recognition Sequence
C(A/C)GtC(G/T)G
GCATGtC
CtTCGAG
GGtCC
CCAN4tNTGG
AtCCGGT
CTGCAtG
CGATtCG
CAGtCTG
GTtAC
CGtG(A/T)CCG
GAGCTtC
GAGtCTC
CCGCtGG
GtTCGAC
t
GATC
GtGNCC
AGTtACT
G(G/A/T)GC(C/A/T)tC
GGCCNNNNtNGGCC
TTtCGAA
GCGATtCGC
GtG(A/T)CC
CCCtGGG
CtCCGGG
TACtGTA
AtCTAGT
GCATGtC
AATtATT
GAGCTtC
GAGtCTC
CCGCtGG
AGGtCCT
CtC(A/T)(A/T)GG
TtCGA
TtTAA
GACNtNNGTC
TtCGA
CCAN4tNTGG
GtTGCAC
ATtTAAT
TtCTAGA
CtTCGAG
(A/G)tGATC(T/C)
CtCCGGG
CCCtGGG
CtGGCCG
CtCCGGG
CCCtGGG
GAANNtNNTTC
Key:
N = A, C, G or T
* = neoschizomer
Notes:
1. The locations of cleavage sites falling outside the recognition site are indicated in
parentheses. For example, GTCTC(1/5) indicates cleavage at:
5′...GTCTCNt...3′
3′...CAGAGNNNNNs...5′
2. DpnI is unique among commercially available restriction enzymes in requiring methylation
of a nucleotide (adenine) in its recognition sequence in order to cut. Therefore, DpnI
cannot be substituted for other enzymes recognizing the GATC sequence (e.g., MboI and
Sau3AI).
3. Although HpaII and MspI recognize the same nucleotide sequence, HpaII is sensitive to
methylation of either cytosine in its recognition sequence, while MspI is sensitive only
to methylation of the external cytosine. These enzymes may not be interchanged for all
applications.
Reference
Roberts, R.J. and Macelis, D. (1991) Nucl. Acids Res. 19 (supp), 2077.