DNA Mass Ladders
M
arligen’s DNA Mass Ladders are designed for accurate estimation of the size and quantity of doublestranded DNA fragments following electrophoresis. Each band contains an exact amount of DNA,
allowing determination of both molecular weight and quantity. The DNA Mass Ladders are conveniently
supplied in loading buffer for direct application to either TAE or TBE agarose gels. The Broad Range DNA
Mass Ladder contains the following 14 fragments: 200, 400, 600, 800, 1000, 1500, 2000, 2500, 3000, 4000,
5000, 6000, 8000 and 12000 bp. The PCR DNA Mass Ladder contains the following eight fragments: 100, 300,
500, 700, 1000, 1500, 2000 and 3000 bp.
ADVANTAGES
Convenient ready-to-load format saves time
Simultaneous estimation of both molecular weight and amount of DNA
Includes the broadest range of fragment sizes in any commercially available product
Includes a brighter reference band for easy orientation
PERFORMANCE CHARACTERISTICS
BROAD RANGE DNA MASS LADDER
Band Size
ng/Band
12,000
8,000
6,000
5,000
4,000
3,000
2,500
60
80
60
50
40
30
25
2,000
20
1,500
15
1,000
100
800
80
600
60
400
40
200
20
FIG. 1. Each lane contains 5 ul of the Broad Range DNA
Mass Ladder run on a 1% agarose gel in 1X TAE and stained
with ethidium bromide. Sharp, well-spaced bands covering a
broad range of fragment sizes from 200 bp up to 12 kb make
determining both the molecular weights and amounts of doublestranded DNA fragments simple and fast.
PCR DNA MASS LADDER
Band Size(bp)
ng/Band
3,000
25
2,000
25
1,500
25
1,000
75
700
25
500
25
300
25
100
25
To Orde r : 866.464.4990
FIG. 2.
Each lane contains 5 ul of the PCR DNA Mass Ladder
run on a 1% agarose gel in 1X TAE and stained with ethidium
bromine. Sharp, well-spaced bands covering a broad range of
fragment sizes from 100 bp up to 3 kb make determining both
the molecular weights and amounts of double-stranded PCR
products simple and fast.
M
301.874.4990
[email protected]
Better research by design
DNA Mass Ladders
STORAGE CONDITIONS
Stable for at least one year at -20°C or 6 months at 4°C. Aliquot product if necessary to avoid repeated
freeze-thaw cycles.
COMPOSITION OF STORAGE BUFFER
10 mM Tris-HCL (pH 8.0), 1 mM EDTA, 8% sucrose, 0.05% bromophenol blue.
ORDERING INFORMATION
To Orde r : 866.464.4990
Item Name
Broad Range DNA Mass Ladder
PCR DNA Mass Ladder
Size
100 applications
100 applications
M
301.874.4990
[email protected]
Better research by design
Markers and Ladders
Catalog No.
11447-100
11446-100