Antibiotics for Mammalian Cell Culture
Product Name
Amphotericin B
Amphotericin B-Solubilized
(Approx. 45%)
Amphotericin B(250 mg/ml solution)
Ampicillin
Antibiotic Antimycotic
Solution (100x)
(10,000 units penicillin,
10 mg streptomycin,
and 25 µg amphotericin B per ml)
Antibiotic-Antimycotic
(100X)
Cephalothin
Dihydrostreptomycin
Erythromycin
Gentamicin Sulfate
Gentamicin Sulfate
(10 mg/ml solution)
Gentamicin Sulfate
(50 mg/ml solution)
L-Glutamine-PenicillinStreptomycin Solution
(200 mM L-glutamine,
10,000 units penicillin,
and 10 mg streptomycinper ml)
Kanamycin Monosulfate
Kanamycin Sulfate
(10 mg/ml solution)
Kanamycin Sulfate
(50 mg/ml solution)
Lincomycin HCI
Neomycin Sulfate
Neomycin Sulfate
(10 mg/ml solution)
Nystatin
(5,000 units nystatinper mg)
Nystatin
Nystatin Suspension
(10,000 units nystatinper ml)
Paromomycin Sulfate
Penicillin-G (potassium salt)
Penicillin-G (sodium salt)
Penicillin-G (sodium salt)
Penicillin-Streptomycin
Solution (5,000 units
penicillin-G and 5 mg
streptomycin per ml)
Penicillin-Streptomycin
Solution (10,000 units
penicillin-G and 10 mg
streptomycin per ml)
Penicillin G-Streptomycin
Penicillin-Streptomycin-Neomycin
solution (5,000 units penicillin-G, 5
mg streptomycin and 10 mg
neomycin per ml)
Penicillin-G StreptomycinNeomycin
Phenoxymethylpenicillinic
Acid (potassium salt)
[Penicillin V]
Polymyxin B Sulfate
SpectinomycinDihydrochloride
Spectromycin Sulfate
Spectromycin Sulfate
TetracyclineHydrochloride
Tylosin Tartrate(8 mg/ml soln.)
Product
Number
A 2411
A 9528
Microbial spectrum
Gram
Gram
(+)bacteria (-)bacteria Yeasts
Molds
•
•
•
•
A 2942
Suggested
Working Conc.
Mycoplasma
•
•
2.5 mg/L
5.6 mg/L
(of solid)
10 ml/L
A 0166
A 5955
•
•
•
•
•
•
100 mg/L
10 ml/L
A 7292
•
•
•
•
10 ml/L
C 3050
D 5155
E 5389
G 1264
G 1272
•
•
•
•
•
•
•
•
•
•
•
•
100 mg/L
100 mg/L
100 mg/L
50 mg/L
5 ml/L
G 1397
G 1522
G 1146
G 6784
•
•
•
1 ml/L
•
•
K 1377
K 0129
•
•
•
•
•
•
100 mg/L
10 ml/L
K 0254
K 0379
L 2774
N 6386
N 1142
•
•
•
2 ml/L
•
•
•
•
•
10 ml/L
100 mg/L
50 mg/L
5 ml/L
N 6261
•
•
N 4014
N 1638
•
•
•
•
2.5 X 105U/L
(50 mg/L)
50 mg/L
24 ml/L
P 5057
P 7794
P 3032
P 3414
P 0906
P 4458
•
•
•
•
•
•
100 mg/L
100,000 U/L
100,000 U/L
100,000 U/L
20 m/L
P 0781
P 7539
P 4333
•
•
10 ml/L
P 3539
P 9032
•
•
•
•
10 ml/L
10 ml/L
P 4083
P 3664
•
•
10 ml/L
P 4807
•
P 4932
S 4014
S 9137
S 0890
T 7660
T 3397
•
•
•
•
•
100,000 U/L
•
•
•
•
•
•
50 mg/L
7.5-20 mg/L
100 mg/L
10 mg/L
10 mg/L
1 ml/L
Antibiotics for Plant Cell Culture
Working Concentration
(µg/ml) and
applications 1
Gram (+)
Bacteria
Gram (-)
Bacteria
C 3416
*
•
Cefotaxime
C 7039
*
•
Chloramphenicol
C 1919
•
•
*
*
10-35 (TS)
Erythromycin
G 418
Gentamicin Sulfate
Hygromycin B
Kanamycin
E 4514
G 1279
G 6896
H 9773
K 4378
•
•
*
*
*
•
•
•
•
•
32
10-500
up to 250 (CC)
100-200
16 (SSC);
up to 40 (CC, PR, EC)
Neomycin
Paromycin
Penicillin G, Potassium
Penicillin G, Sodium
Rifampicin
N 3144
P 8692
P 8306
P 8431
R 7382
•
•
•
•
•
*
*
*
*
•
•
Streptomycin sulfate
Vancomycin
S 0774
V 1130
*
•
•
*
Product
ANTIBIOTICS
Carbenicillin
Mycobacteria
Mycoplasma
Product
Number
Yeasts
Molds
up to 500
(CC, PR, EC)
up to 500
(CC, PR ,EC)
up to 1,000
up to 100
up to 100
50 (TS);
up to 25
(PC, TEC)
< 16
up to 10 (PC);
up to 40
(CC, EC, STM)
ANTIMYCOTICS
Amphotericin B
A 6804
•
•
up to 5
(CC, EC, STM)
Nystatin
N 9767
•
•
16 (SSC);
up to 40
(CC, EC, STM)
Pentachloronitrobenzene
Thiabendazole
P 8556
T 5535
•
•
• -Effective against most species
* -Effective against certain species
1
Application Key: CC = callus culture; PR = plant regeneration; EC = cotyledon, hypocotyl, or leaf disc culture; TS = transformant
selection; SE = somatic embryogenesis; SSC = stem selection culture; PC = protoplast culture; TEC = tuber explant culture; CC = cell
culture; STM = shoot tip micropropagation
Antibiotic Selection Agents for Cell Culture
Product
Number Mode of Action
Product Name
Actinomycin D
Complexes with DNA and interferes with
A 9415
RNA synthesis
Bleomycin Sulfate
Complexes with DNA, causing strand
B 8416
scissions
Chloramphenicol
Inhibits elongation at peptidyl transferase
C 3175
5 µg/ml
G 418
A 1720
100-800 µg/ml
G 418
G 8168
(50 mg/ml solution)
Hygromycin B
H 3274
Mitomycin C
Mycophenolic Acid
M 4287
M 3536
Puromycin•HCI
P 8833
Blocks polypeptide synthesis and inhibits
chain elongation
Blocks polypeptide synthesis and inhibits
chain elongation
Blocks polypeptide synthesis and inhibits
chain elongation
Inhibits nucleic acid synthesis
Blocks inosine monophosphate
dehydrogenase in guanosine
monophosphate pathway
Inhibits protein synthesis
Suggested Working Conc.
1 µg/ml
10-100 µg/ml
100-800 µg/ml
10-50 µg/ml
25 µg/ml
10-100 µg/ml