GUNDERSEN HEALTH SYSTEM LABORATORY SPECIMEN REQUIREMENTS
For complete listing, refer to Gladiator /Internal Sites/Department Web sites/Laboratory/Specimen Requirements or call 53999
Note: The first tube listed is the tube of choice, but second/third is acceptable.
ABORH – LAV
Acetaminophen/Tylenol –
Green/SST/PRT
Albumin – GRN/SST/PRT
*Alcohol (Medical) – Grey/
GRN/SST- do not use
alcohol wipe see note on back
Alfa-Fetoprotein(AFP) - SST
Alkaline Phosphatase –
GRN/SST
A1C (Glycosylated Hgb) –
LAV
Ammonia (NH3) – LAV on ice
Amylase – GRN/SST
ANA – SST
Anti-DNA – SST
Antibody Screen – 6ml LAV
Ascorbic Acid –no gel Na/Hep
GRN on Ice -12-14 hr. Fast &
Protect from light
AST (SGOT) – GRN/SST
B12- SST – protect from light
Beta HCG (Quant) – GRN/SST
Bilirubin Grn/SST (all – protect
from light)
BNPNT (B Type Natriuretic
Peptide) GRN/SST
BUN – GRN/SST
C Reactive Protein (CRP)
GRN/SST
C3 – SST
C4 – SST
CA125 – SST
CA19-9 - SST
Calcium – GRN/SST
Calcium Ionized – 4ml full SST
Carotene – PRT
CBC – LAV
CEA – SST
CH50 – SST/PRT
Chloride – GRN/SST
Cholesterol – GRN/SST
CMVMG – SST
CO2 – GRN/SST
Cold Agglutinin – SST
Cortisol – SST/GRN
CPK – GRN/SST
Lab 1360.1 Revised 5/2015
Creatinine – GRN/SST
Cryofibrinogen – 4-2.7ml BLU4 hr. Fast & Keep Warm
Cryoglobulins –4-4ml SST
&4hr. Fast
Keep warm
D-Dimer – BLU
Depakote (Valproic Acid) –
GRN/SST/PRT
Digoxin – Green/SST/PRT
Dilantin (Phenytoin) –
Green/SST/PRT
EBVGM – SST
Direct Coombs – LAV
Electrolytes – GRN/SST
ENA – SST
ESR/Sed Rate – BLK/LAV
Estradiol –GRN/SST
Ethylene Glycol – Grey
Factor’s - BLU
Ferritin – GRN/SST
Fibrinogen – BLU
Folate – SST protect from light
FSH – SST
Gamma GT (GGT) –
GRN/SST
Gastrin – SST/PRT –
Fasting
Gentamicin – GRN/PRT/SST
Glucose – GRN/SST Must be spun
within ½ hour or put on ice
Haptoglobin – SST
HCG/ Pregnancy – SST
HDL Cholesterol – GRN/SST
Hematocrit (HCT) – LAV
Hemoglobin (HGB) – LAV
Hemogram (HEMG3) – LAV
Hepatitis (All) – 2 4ml SST
HIV – SST- signed consent
Homocysteine – LAV – on ice
4 hrs fasting
H-Pylori – SST/PRT
IFLC - SST
INR (PT) – BLU – fill line
Iron and Iron Binding –
SST/GRN
Lactic Acid – Grey on ice must be
off cells within 15 min. of draw
LAX Enceph Titer – SST
LDH – GRN/SST
Lead – TAN (metal free
EDTA)/LAV
LH – SST
Lidocaine – PRT
Lipase – GRN/SST
LPA (Lipoprotein Analysis) –
GRN/SST – 9hr. Fast
Lithium – SST/PRT
Lymes – SST
Magnesium (MG) – GRN/SST
Methotrexate – PRT (Protect from
Light)
Methylmalonic Acid – PRT/SST
Monotest – LAV
Osmolality – GRN/SST
P2Y12 – Use Kit
Peripheral Smear – LAV (<12
hours old)
PFA – 2–2.7ml BLU (DO NOT
SEND IN TUBE OR SPIN)
Phenobarbital –Green/SST/PRT
Phenytoin/Dilantin –
Green/SST/PRT
Phosphorus – GRN/SST
Platelet (PLT) – LAV
Potassium (K) – GRN/SST (must be
spun within ½ hour)
Primidone – PRT
Procainamide – PRT
Progesterone – GRN/SST
Prolactin – GRN/SST
PSA – SST/GRN
Protein Electrophoresis (PES) –
SST
PT (INR) – BLU – fill line
PTT – BLU – fill line
Reticulocyte Count (RETIC) –
LAV
Rhuematoid Factor (RH) – SST
RMISC/OTHER – call 53433
Rubella – SST
Rubeolla – SST
Salicylate/Aspirin –
Green/SST/PRT
Sed Rate (ESR) – BLK/LAV
(min 3.0 ml in lav) Must be
at fill line
Sickle Cell – LAV
Sodium (NA) – GRN/SST
T3 – GRN
T4 Free (FT4) – GRN/SST
Tegretol/Carbamazepine –
GRN/SST/PRT
Testosterone (Total Only) –
GRN/SST
Theophylline/Aminophylline –
GRN/SST/PRT
Thyroid Antibody – SST
Tobramycin – PRT/LAV
Total Protein – SST
Triglycerides – GRN/SST
Troponin – GRN (draw own
tube if chems also ordered)
TS (Type and Screen) – 6ml LAV
BB#, 1ST initial, full last name
TSH – GRN/SST
TSTT1 - SST
Tylenol/Acetaminophen –
Green/SST/PRT
Uric Acid – GRN/SST
Valproic Acid – Green/SST/PRT
Vancomycin – Green/SST/PRT
Varicella (VZIG) – SST
VDRL – SST
Vit D 25HYD – SST
Vit D, 1, 25-HYD – PRT/SST
4 hr. Fast preferred
VITE – PRT only, 12-14 hr. Fast
& Protect from Light
WBC – LAV
See Back Side for Notes
Gundersen Health System Laboratories – Specimen Requirement Notes
If you have questions regarding specimen collection or add-ons, Call 53999
Order of Draw (Vacutainer/Syringe)
and Anticoagulant in Tube:
Tube Type Abbreviations:
Blood Cultures
Blue/Black
Sodium Citrate
Pumpkin/Plain Red No anticoagulant
Green
Heparin
Lavender
EDTA
Grey – Sodium Fluoride/Potassium Oxalate
Gold (aka pumpkin)
PRT
Blue
Green
Black
BBL
SPC
SST/Serum w gel
Plain Red – no gel
Blu
Grn
BKT
Blood Bank (6 ml LAV)
Special – see comments on label
Order of Draw for Microtainer Collection (capillary puncture-from heel or finger):
Lavender
Green
Grey
Plain Red/Yellow
Do not use the 0.9 MiniCollect tube for adults unless provider is notified. This tube is for neonates. When collecting blood for the 0.9 mini-collect tube, it must be venous
blood – not capillary.
Protocols:
GI Profile:
Green
Lavender
Hip Procol:
Blue
SST
Green
Lavender
Lavender (6ml)
Amylase/Alk Phos
SGOT/Bili
CBC
PT INR/PTT
NA/K/BUN/Glucose
CBC
TS
ED Profile:
Green
Lavender
CCU Protocol:
Blue
Green
Green
Lavender
6 ml Lavender
Creat/Gluc/
NA/K/BUN
CBC
PT INR/PTT
Troponin
NA/K/Gluc/Creat
CBC
BB Hold
Trauma Protocol:
Blue
PT INR/PTT
Green
Creat/NA/K
Glucose/Amylase
Lavender
CBC
TS
Lav (6ml) BB ARMBAND
ETOH
Grey
Lactic Acid
Grey - on Ice
VBG
BG syringe-Draw Last
NOTES:
 All tubes with additives must be gently mixed 6 – 8 times
 Blue tubes must be filled to the minimum fill line (groove in tube) – others may be half full unless specifically required to be full
 Fill tubes from syringes immediately after draw (gently mix)
 Blood Bank Tubes MUST have BB# and 1st initial-full last name of phlebotomist if for possible transfusion (All Inpatients)
 Blood Cultures require a 30 second Chloraprep scrub – Alcohol for infants less than 2 months of age
 Tourniquet should not be left on longer than 2 minutes (closer to 1 minute is preferable)

Note regarding ETOH: May add ETOH onto unopened SST or Green. Enter Changed Specimen Type in LIS. Enter Comment: add-on ETOH/ not collected per ETOH protocol.
Lab-1360.1 (back) Revised 5/2015
Reviewed by: ___________________________ Date: ________________________________