Review: Redi Needham Spallanzani Pasteur Leeuwenhoek why their discoveries disproved spontaneous generationi Sep 1311:35 AM The Microscope (pages 12 14) the microscope consists of a lens or combination of lenses that produce enlarged images of small objects earliest microscopes were a single curved lens mounted on a metal plate (similar to a magnifying glass) Sep 1311:44 AM 1 later the compound light microscope was developed it had two lenses (ocular and objective) and total magnification was determined by multiplying the power of the ocular by the objective newer microscopes had improved resolution which refers to its ability to distinguish between objects that are close together Sep 1311:48 AM Types of Microscopes: 1. Compound microscope uses light and a combination of lenses to enlarge small objects maximum magnification of about 2000X Sep 1311:51 AM 2 2. Transmission Electron Microscope: (TEM) directs a beam of electrons through an object object must be thin and treated with chemicals for an accurate image to be obtained maximum magnification of to 50,000,000X Sep 147:44 AM 3. Scanning Electron Microscope: (SEM) uses electrons to take pictures of the specimen and displays it on a television screen uses the whole organism to produce almost 3d images maximum magnification is about 500,000X Sep 147:49 AM 3 What is this?________________ Which microscope was used? ___________________________ See pages 20 21 for more information Sep 147:55 AM Parts of a Microscope a.Ocular Lens: magnifying lens you look through b. Ocular Tube: holds the ocular lens c. Coarse Adjustment Knob: move the stage up and down to bring an object into focus d. Fine Adjusting Knob: brings an object into sharper focus (use with medium/high) e. Revolving Nosepiece: holds two or more objective lenses which you can rotate or change f. Arm: connects the body tube with the base (use it for carrying) g.Objective Lens: magnify the object on a slide h. Stage Clips: hold the slide in place Sep 147:56 AM 4 i. Stage: platform that supports the slide j.Disk diaphragm: controls the amount of light that reaches the object k. Inclination Joint: pivots the microscope l. Light Source (mirror): directs light through the diaphragm to help with observation m. Base: supports the microscope Sep 143:06 PM Readings: Pages 12 21 Activities: Complete handout on microscope Sep 143:09 PM 5
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