The study of the microbial content of the air has become increasingly more significant in the past decade as the need for “contamination free” environments have become more apparent. Biological aerosols have been defined as viable biological contaminants occurring as solid or liquid particles in the air. These particles can vary in size from viruses less than 0.1 micron in diameter to fungal spores 100, or more microns in diameter. They may occur as single, unattached organisms or as aggregates. Viable particle samplers have been used to collect and study aerobic species of bacteria and fungi. Even though many viable samplers, including the Tisch Environmental sampler, will collect some virus particles, there is no convenient, practical method for the cultivation and enumeration of these particles. There are two constraints on all viable particle samplers, first, the particle must be separated from the air for any viability study, and second, the ability to reproduce (viability) must be demonstrated. The purpose of this manual is to outline proper methods for the study of biological aerosols using the Tisch Environmental Viable Particle Samplers. TE-10-800 SIX STAGE VIABLE IMPACTOR OPERATING AT 28.3 lpm Stages Mic. Cut-Point 1 7.1 and above 2 4.7 to 7.1 3 3.3 to 4.7 4 2.1 to 3.3 5 1.1 to 2.1 6 0.65 to 1.1 TE-10-820 Same as TE-10-800, except a 12 Volt DC Pump/Motor, in place of 115 Volt or 220 Volt AC Pump TE-10-830 Same as TE-10-800, excludes Vacuum Pump Assembly TE-10-850 Two Stage Ambient Viable Sampler excludes Vacuum Pump Assembly TE-10-860 Same as TE-10-850, includes Vacuum Pump Assembly and Carrying Case TWO STAGE VIABLE IMPACTOR OPERATING AT 28.3 lpm Stage Mic. cut-point 1 8.0 and above 2 1.0 to 8.0 TE-10-890 Single Stage (N6) Ambient Viable Sampler includes One Aluminum Orifice Stage, Silicone O-ring Gaskets, Vacuum Pump Assembly and Carrying Case complete TE-10-880 Same as TE-10—890 Except Vacuum Pump Stage Mic. Cut-Point 6 0.65 and above (nominally to 22 micrometers)
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