Agenda
Overview
Introduction to OpenLAB ELN
– Benefits of an ELN platform
Case Study
– MircoRNA Detection
Demonstration
– Example workflow
Two main paper-based laboratory workflows
Discovery Workflow
• Scientific collaboration drives new
discoveries
• Need for flexible work processes
• Intellectual property protection is critical
Process Workflow
• Standard operating procedures
• Mainly routine testing
• GxP required
Test
Test
Challenges in the Laboratory
• Data Overload from disparate data sources
• Record Keeping Lab Notebooks/ Original Data
• Data Integrity / Validation
• Access to Information for Accelerated Publication
• Need for Increase Collaboration
• Intellectual Property Protection
• Experienced and Inexperienced Researchers
• Downsizing and Turn-over of Employees,
Advancements in Instrumentation Create a Data Deluge
More results…
• What was the best method used?
• Has anyone done this before?
• How did I do this?
• Where are my results?
Taking Advantage of Laboratory Informatics
• Search across the organization
• Share methods and results
• Report experiment details
• Secure IP and protect results
Spend less time documenting in your paper
notebook and more time in the lab…
How Informatics can help Laboratories
manage their information
• Productivity gains through paperless solutions
• Making data and information available when, where, and how you need it
• Streamline and optimize laboratory workflow
• Managing and protecting your intellectual property
• Working smarter -- let the technology work for you
Medicinal
Chemistry
Synthetic
Chemistry
Drug Discovery
Agilent OpenLAB Informatics Portfolio
OpenLAB ECM
OpenLAB CDS
OpenLAB ELN
Data Management
Chromatography Data System
Electronic Lab Notebook
Scientific Data Management
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Data Archiving
Agilent WorkStation Aware!
Instrument Vendor Neutral
Time Stamp/Audit Trail
Report Capture
Viewers, Filters, Converters
E-Signatures
Regulatory Compliance
TNF Conversion (LTDA)
Workstations, Client-Server
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ChemStation, EZChrom Elite
Multi-Vendor
Workstation – Client/Server - Web
OpenLAB ECM Intelligent Reporter
Regulated and Non-Regulated
Capture, Organize, Collaborate
• Smart, high productivity alternative
to paper notebooks
• IP Protection and Compliance
• Simplifies and accelerates lab work
• Smart Import & Dynamic Forms
• Simple Web Based Client
• Analytical Request Module
Agilent OpenLAB ELN
• Streamlines data capture through an easy-to-use web interface
• Simplifies and accelerates data searching and retrieval
• Eliminates need to repeat experiments unnecessarily
• Facilitates rich collaboration across the lab and organization
• Integrates seamlessly with existing processes and procedures
• Safeguards your intellectual property
Integrates with Agilent OpenLAB ECM to manage your information end-to-end
An ELN Tailored to Your Working Environment
• Quickly setup an experiment and
capture data.
• Mirror your workflow with experiment
templates, forms and form fields
• Ability to search - review experimental
data across multiple researchers
• Complete and transparent auditing
activities
Focus on Analytical Chemistry
• Generate quickly reports across
multiple samples and techniques
• Reduce cycle time from request to
result
– Check status, queue requests
– Easy tracking of procedures and details
– Reduce time for routing document
approval
• Import directly from instruments
– ChemStation, Empower
• Capture images, chromatograms,
spectra; fully searchable annotations
• Setup experiments quickly with
standard sentences and templates
Focus on Biology
– Cell biology
– Genomics
– Proteomics
– Toxicology
– Molecular biology
• Rich text environment for free-form data
entry
• Insert images, searchable annotations
• Re-use protocols and methods
• Clone previous experiments
• Link to external sources (e.g. websites,
documents)
• Import files, data and results from other
applications
– GeneSpring, MassHunter,
VectorNTI…
Focus on Synthetic Chemistry
• Search and re-use synthesis knowledge
from across the lab or organization
• Plan, search and design reactions
• Access commercial sourcing and
registration systems
• Create favorites for commonly used
reagents and solvents
• Manage batches, split samples and
submit for analysis directly from the
experiment
Agenda
Overview
Introduction to OpenLAB ELN
– Benefits of an ELN platform
Case Study
– MircoRNA Detection
Demonstration
– Example workflow
Case Study – MicroRNA Analysis
• Measure miRNA expression in
circulating neutrophils before and
after brief exercise
• Techniques used:
- Neutrophil isolation
- RNA extraction
- miRNA microarrays
• Document sample preparation,
method details, assay kits and
reagents used
• Collect results, analyze with
GeneSpring, compare analysis
• Make conclusions
**Evidence for microRNA Involvement in Exercise-Associated Neutrophil Gene Expression Changes
Shlomit Radom-Aizik, Frank Zaldivar, Jr., Stacy Oliver, Pietro Galassetti, and Dan M. Cooper
Articles in PresS. J Appl Physiol (January 28, 2010). doi:10.1152/japplphysiol.01291.2009
Case Study – MicroRNA Analysis
Quickly enter protocol details with Experiment Templates
Prompt for required
values
Fast data entry with
pick lists
Quickly add one or
more Forms to an
experiment
Case Study – MicroRNA Analysis
Experiment results in OpenLAB ELN
Create tables or embed
MS Excel spreadsheets
Add free text, urls and links
to external applications
Link to the original
data files
Insert and
annotate images
Summary
Increase productivity
– Easily document and manage experiments
– Share work in a collaborative effort
– Support multiple disciplines with a single
solution
– Integrate with existing Information Systems
Protect Intellectual Property
– Internally within the system
– Externally using Surety AbsoluteProof
OpenLAB DEMO