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RIGAUT, G., A. SHEVCHENKO, B. RUTZ, M. WILM, M. MANN et al., 1999 A generic protein purification method for protein
complex characterization and proteome exploration. Nat Biotechnol 17: 1030-1032.
ULE, J., K. JENSEN, A. MELE and R. B. DARNELL, 2005 CLIP: a method for identifying protein-RNA interaction sites in living
cells. Methods 37: 376-386.
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