Respiration Lesson Description The following would be covered: Define cellular respiration Discuss the role of glycolysis, Kreb’s cycle and Oxidative phosphorylation in aerobic respiration Define anaerobic respiration Explain Alcoholic fermentation and Lactic acid fermentation in muscles Look at the role of anaerobic respiration in industry Compare aerobic respiration and anaerobic respiration Respiration – the process Why do organisms need to respire? All living things need energy Energy in the form of… Food=chemical energy Cell use energy in the form of ATP Cells recycle ATP Where does all this take place? Mitochondria Explain this organelle structurally suited for respiration. Double membrane which is permeable to gases and water Inner membrane folded to increase surface area for attachment of respiratory enzymes What is respiration? Cellular Respiration is the process that releases energy by breaking down food molecules in the presence of oxygen. 6O2 + C6H12O6 6CO2 + 6H2O + Energy Oxygen + Glucose Carbon Dioxide + Water + Energy There are three stages in aerobic respiration – 1. Glycolysis 2. Kreb’s Cycle (Citric Acid Cycle) 3. Electron Transport Chain Glycolysis: This occurs in the cytoplasm of the cell. Glucose is broken down into pyruvic acid and energy-rich rich hydrogens are given off. The hydrogens move into the mitochondria to be used in oxidative phosphorylation. Two ATP molecules are produced during glycolysis. The Krebs cycle breaks down the pyruvic acid completely into energy-rich hydrogen and carbon dioxide. The hydrogen will be used in oxidative phosphorylation and the carbon dioxide will be released eventually from the lungs. Oxidative phosphorylation: takes the energy from the energy-rich hydrogens to make ATP The energy depleted hydrogens combine with oxygen to make water. This is either released as water vapour or excreted with the urine. A Summary of the process: Anaerobic Respiration What happens if oxygen does not reach the mitochondria? Anaerobic respiration occurs when glucose is broken down and there is no oxygen present. Very little energy is produced. There are two types of anaerobic respiration :- Question One Why is respiration important to organisms? Maintains the oxygen balance in the atmosphere. Maintains the carbon dioxide balance in the atmosphere. Providing energy to heterotrophs. 2. Draw a labelled diagram to illustrate the general structure of the mitochodria. 3. Study the diagram below and then answer the questions that follow. (Nated SG p1 2010) 1. State ONE function of the rubber stopper in tube A. (1) 2. Name the (i) biochemical process that is taking place in tube A. (ii) (iii) 3. 4. 5. 6. gas bubbles in tube B. liquid X in tube B. Name THREE products formed in the biochemical process that is taking place in tube A. (3) Describe how you would set up a control for this investigation. (2) Give ONE reason why the process illustrated in the diagram is economically important. (1) State ONE reason why it would have been better to use a thermos flask instead of a test tube. (2) 1 - Prevents atmospheric air from entering/escape the test tube/make test tube airtight 2 (i) anaerobic respiration/fermentation (ii) carbon dioxide (iii) lime water /bromothymol blue solution 3 - alcohol - carbon dioxide - ATP /energy 4 Set up the apparatus as shown but do not put in yeast in test tube A 5 - beer making/wine making/produce alcohol - baking/form CO2 (mark first ONE only) 6 - to keep temperature constant (Nated HG p1 2012) 4. A group of learners investigated whether all organisms release carbon dioxide. They set up the experiment as shown in the diagram below. All the test tubes were kept in light and they used an indicator to show the different concentrations of carbon dioxide. The following colour changes of the indicator revealed the levels of carbon dioxide present: Normal amount of carbon dioxide: Indicator turns orange Low amount of carbon dioxide: Indicator turns purple High amount of carbon dioxide: Indicator turns yellow The table below shows the results obtained in each test-tube. 1. 2. 3. 4. 5. 6. What is the purpose of test tube C in the investigation? Give ONE reason why it was necessary to seal each test tube with a rubber stopper. What gas do you think would increase in concentration in test tube A? Explain your answer. Explain the results of each test tube after a few hours of the investigation. Predict what colour the indicator would be if the learners set up a fourth test tube with worms and leaves. Explain your answer to 5 above. Anaerobic Respiration in Industry Yeast is used widely in Biotechnology. It is used in the fermentation of alcohols and the production of bread. 5. Define cellular respiration Process whereby organic compounds are broken down using oxygen to release energy in the form of ATP 6. List the reactants and the products of cellular respiration in both chemical equation and words. 6O2 + C6H12O6 6CO2 + 6H2O + Energy Oxygen + Glucose Carbon Dioxide + Water + Energy 7. Tabulate the differences between respiration and photosynthesis. Respiration Occurs in cytoplasm and mitochondria Oxygen consumed Catabolic –uses energy Energy, CO2,H2O produced Light not essential Photosynthesis Occurs in chloroplast Carbon dioxide confused Anabolic – makes energy Organic molecules produced Light essential 8. Compare aerobic and anaerobic respiration. Aerobic respiration occurs in the presence oxygen and creates a maximum of 38 ATP, while anaerobic respiration occurs in the absence of oxygen and creates a maximum of 2 ATP. The products of aerobic respiration are CO2, energy and water vapour while the products of anaerobic respiration are CO2, little energy and alcohol al or lactic acid June Exam 2013 Ahmed Timol Secondary 9. The following diagram shows the apparatus used in an experiment. Seeds of the pea plant were soaked in water and placed into 3 identical glass bottles. A fourth empty bottle was also used. A Carbon dioxide sensor was placed into each of the bottles. One bottle with seeds was place into a freezer, room and oven. Answer the questions that follow. 1 What process is being investigated in this experiment? (1) 2 Write down an aim for this experiment (2) 3 Is light necessary for this process? (1) 4 Why was one bottle left empty (without seeds?) (1) 5. Why was it necessary to soak the seeds in water? (1) 6. The table below indicates the results of the experiment. Use this data to draw a graph. CONDITION AMOUNT OF CO2 IN AIR (%) No seeds 0.03 Freezer 0.04 Room temperature 0.1 Oven 0.03 (10) 1 Cellular respiration 1 2 To show/determine the effect of temperature on respiration 2 3 No 1 4 As a control /to know the normal amount of CO2 in air 1 5 So that the seeds start to germinate/grow/respire 1 6 Amount of CO2 in bottles put under different conditions. AMOUNT OF CO2 IN AIR (%) 0.12 0.1 0.08 0.06 0.04 0.02 0 No seeds Freezer Room temperature CONDITION CRITERION MARKS Axes labels 2 y-axis scale 1 Labelling of bars/key 1 Heading 1 Oven Plotting 4 Type of graph 1 (TOTAL =10) .
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