GE Healthcare
Membrane chromatography
using ReadyToProcess™ Adsorber
Selection guide
ReadyToProcess Adsorber is a range of chromatography membranes primarily used for polishing
of monoclonal antibodies (MAbs) and other biomolecules. For convenient and reliable scale-up,
the ReadyToProcess Adsorber product line is available from 96-well screening formats for highthroughput process development (HTPD), all the way to sizes for large-scale production.
This document provides an overview of which chromatography system is suitable for operating
a certain ReadyToProcess Adsorber capsule.
Recommended systems
Other systems and pumps
System choice considerations
ReadyToProcess Adsorber is primarily
recommended to be operated by ÄKTA™
chromatography systems to enable
scalability from process design to large-scale
manufacturing. The ÄKTA platform provides
process control and data logging for batch
documentation. It is also fully scalable and
covers all major chromatographic and cross
flow filtration techniques.
Inline Conditioning systems
ReadyToProcess Adsorber membrane
capsules can be operated by Inline
Conditioning systems—automated liquid
handling systems built for large-scale
biopharmaceutical manufacturing. Inline
Conditioning systems act as a buffer
preparation unit and enable automatic inline buffer formulation using concentrated
stock solutions. For increased flexibility,
the Inline Conditioning system can have
integrated chromatography functionality.
Flow capability vs required flow rate
When selecting a system for running
membrane chromatography, the first aspect
to consider is the flow capability of the system
versus the required flow rate for running the
membrane. Recommended flow rates are
30 and 5 membrane volumes (MV)/min for
ReadyToProcess Adsorber membranes with
4 and 8 mm bed height, respectively. However,
the chromatographic performance of
membranes is often maintained even at lower
flow rates and a system with a lower flow rate
than the recommended can therefore be used.
ÄKTA lab-scale systems
ÄKTA start, ÄKTA pure, and ÄKTA avant
systems are recommended for operating
small-scale ReadyToProcess Adsorber
membrane capsules.
ÄKTA pilot and production systems
ÄKTA ready, ÄKTApilot™, and ÄKTAprocess™
systems are recommended for
manufacturing purposes. They offer
high process control, extensive batch
documentation, and more.
Peristaltic pumps
ReadyToProcess Adsorber membrane
capsules can also be operated by simple
peristaltic pumps, like the Watson-Marlow™
520 series. However, peristaltic pumps do
not provide the same process control and
data logging functionality as the ÄKTA
systems.
Possible flow rates span between 1 and
30 MV/min for membranes with 4 mm bed
height. For membranes with 8 mm bed height
flow rates between 1 and 5 MV/­min can be
used. The possibility to run the membranes at
lower flow rates greatly simplifies the system
selection.
Dilution effect considerations
Dilution effects should always be
considered when selecting a system
to run ReadyToProcess Adsorber
membranes. The dilution effect is
usually minor for flow-through (FT)
applications, but more significant for
for bind-elute (B/E) operation, especially
when preparing scale-up.
Chromatography membranes can be
run at high flow rates and large systems
can therefore be used to run a wide
variety of membrane sizes. A larger
system and void volume will, however,
result in an increased dilution effect.
For further assistance, please contact
a GE Healthcare chromatography
application specialist.
Bed height (mm)
Adsorber Q Jumbo 8 mm
Adsorber S Jumbo 8 mm
Adsorber Phen Jumbo 8 mm
Adsorber Q 1.2 8 mm
Adsorber S 1.2 8 mm
Adsorber Phen 1.2 L 8 mm
Adsorber Q 800 8 mm
Adsorber S 800 8 mm
Adsorber Phen 800 8 mm
Adsorber Q 400 8 mm
Adsorber S 400 8 mm
Adsorber Phen 400 8 mm
Adsorber Q 150 8 mm
Adsorber S 150 8 mm
Adsorber Phen 150 8 mm
Bind-elute
4 (recommended flow rate 30 MV/min)
8 (recommended flow rate 5 MV/min)
0.08 mL
1 mL
75 mL
200 mL
400 mL
600 mL
2.5 L
0.08 mL
3 mL
150 mL
400 mL
800 mL
1.2 L
5L
Recommended flow rate (mL/min)
2.4
30
2250
6000
12 000
18 000
60 000
2.4
15
750
2000
4000
6000
25 000
Recommended flow rate (L/h)
0.14
1.8
135
360
720
1080
3600
0.14
0.9
45
120
240
360
1500
Volume
Maximum pressure (bar*)
4
System
Flow rate
Detectors
ÄKTA start
0.5–5 mL/min
Flow, pressure, UV, conductivity
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ÄKTA pure 25
0.001–25 mL/min
Flow, pressure, UV, conductivity, pH
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ÄKTA pure 150
0.01–150 mL/min
Flow, pressure, UV, conductivity, pH
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ÄKTA avant 25
0.001–25 mL/min
Flow, pressure, UV, conductivity, pH
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ÄKTA avant 150
0.01–150 mL/min
Flow, pressure, UV, conductivity, pH
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ÄKTApilot
4–800 mL/min
Flow, pressure, UV, conductivity, pH
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ÄKTA ready, with Low Flow Kit
3–175 L/h
Flow, pressure, UV, conductivity, pH
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ÄKTA ready, with High Flow Kit
7.5–510 L/h
Flow, pressure, UV, conductivity, pH
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4–180 L/h
Flow, pressure, UV, conductivity,
pH electrode (optional)
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ÄKTAprocess, 10 mm polypropylene
or 1/2 in stainless steel
Adsorber Q Nano 8 mm
Adsorber S Nano 8 mm
Adsorber Phen Nano 8 mm
Flow-through
Recommended application
ÄKTAprocess, 6 mm polypropylene
or 3/8 in stainless steel
Adsorber Phen Pico 4 mm
Adsorber Q 2.5 L 4 mm
Adsorber Q 600 4 mm
Adsorber S 600 4 mm
Adsorber Q 400 4 mm
Adsorber S 400 4 mm
Adsorber Q 200 4 mm
Adsorber S 200 4 mm
Adsorber Q 75 4 mm
Adsorber S 75 4 mm
Adsorber Q Nano 4 mm
Adsorber S Nano 4 mm
Adsorber Q Pico 4 mm
Adsorber S Pico 4 mm
ReadyToProcess membrane
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15–600 L/h
Flow, pressure, UV, conductivity,
pH electrode (optional)
ÄKTAprocess, 1 in polypropylene
or stainless steel
45–1800 L/h
Flow, pressure, UV, conductivity,
pH electrode (optional)
Inline Conditioning, 6 mm polypropylene
or 3/8 in stainless steel
4–180 L/h
Flow, pressure, UV, conductivity, pH
Inline Conditioning, 10 mm polypropylene
or 1/2 in stainless steel
15–600 L/h
Flow, pressure, UV, conductivity, pH
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Inline Conditioning, 1 in polypropylene
or stainless steel
45–1800 L/h
Flow, pressure, UV, conductivity, pH
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Inline Conditioning, 1 1/2 in stainless steel
500–5000 L/h
Flow, pressure, UV, conductivity, pH
Inline Conditioning, 2 in stainless steel
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1000–10 000 L/h
Flow, pressure, UV, conductivity, pH
Pressure, conductivity, temperature
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Watson-Marlow 520 series, pumpscil 3/8 in,
1.6–3500 mL/min
with ReadyCircuit assemblies
Pressure, conductivity, temperature
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Watson-Marlow 620 series, pumpscil 3/8 in,
0.003–7.2 L/min
with ReadyCircuit assemblies
Pressure, conductivity, temperature
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Watson-Marlow 620 series, pumpscil 1/2 in,
0.004–11 L/min
with ReadyCircuit assemblies
Pressure, conductivity, temperature
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Watson-Marlow 720 series, pumpscil 1/2 in,
up to 760 L/h
with ReadyCircuit assemblies, 2 bar
Pressure, conductivity, temperature
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Watson-Marlow 720 series, pumpscil 3/4 in,
up to 1390 L/h
with ReadyCircuit assemblies, 1 bar
Pressure, conductivity, temperature
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www.gelifesciences.com/readytoprocess
Connector on membrane capsule:
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Watson-Marlow 520 series, pumpscil 1/4 in,
0.7–1500 mL/min
with ReadyCircuit™ assemblies
* 1 bar = 14.5 psi = 0.1 MPa
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 Luer female connector
 3/4 in sanitary clamp or hose barb
 3/4 in sanitary clamp
 1 1/2 in sanitary clamp
GE and GE monogram are trademarks of General Electric Company. ÄKTA, ÄKTApilot, ÄKTAprocess, ReadyCircuit, and ReadyToProcess are trademarks of General Electric
Company or one of its subsidiaries. Watson-Marlow is a trademark of Watson-Marlow Ltd. All other third party trademarks are the property of their respective owner.
© 2015 General Electric Company—All rights reserved. First published Jun. 2015
All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them.
A copy of these terms and conditions is available on request. Contact your local GE Healthcare representative for the most current information.
GE Healthcare Bio-Sciences AB, Björkgatan 30, SE-751 84 Uppsala, Sweden.
29156202 AA 06/2015