Safety Data Sheet
Issuing Date: 12/10/2014
Revision Date: 12/10/2014
SDS PN: 100-411-200
Rev. 01
1. IDENTIFICATION OF THE SUBSTANCE/PREPARATION
AND THE COMPANY/ UNDERTAKING
Product Identifier
Product Name
Part Number(s)
DNA/Polymerase Binding Kit P6 v2
100-372-700
Relevant Identified Uses of the Substance or Mixture and Uses Advised Against
Recommended Use
For use with the Pacific Biosciences PacBio RS. FOR RESEARCH USE
ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Details of the Supplier of the Substance or Mixture
Supplier Address
Supplier email
Emergency Telephone Number
Pacific Biosciences
1380 Willow Road
Menlo Park, CA 94025
U.S.A
www.pacificbiosciences.com
[email protected]
Within USA & Canada: Call CHEMTREC 1-800-424-9300 (reference
CCN# 656805);
Outside USA and Canada: contact [email protected]
2. HAZARDS INDENTIFICATION
Classification of the Substance or Mixture
GHS / CLP Classification:
Health
Not Hazardous
Environmental
Not Hazardous
EU Classification (1999/45/EC): Not a dangerous preparation.
OSHA / WHMIS Classification: Eye, skin and respiratory irritant
GHS/CLP Label Elements: No Labeling required
Other Hazards: None
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Physical
Not Hazardous
3. COMPOSITION, INFORMATION OR INGREDIENTS
NOTE: Most ingredients are non-hazardous. The two listed components below, in the concentrations present
in this kit, are classified as irritants or are listed because they have established occupational exposure limits.
Chemical Name
CAS-No.
EC-No.
EU Classification
(67/548/EEC)
GHS/CLP
(1272/2008)
Classification
Glycerol
56-81-5
200-289-5
Not dangerous
Not hazardous
Acute Tox 4 (H302)
DL-Dithiothreitol
Xn, R22
Skin Irrit 2 (H315)
3483-12-3
222-468-7
Solution
Xi, R36/37/37
Eye Irrit 2A (H319)
STOT SE 3 (H335)
See Section 16 for the full text of abbreviations and R and H phrases in this section.
Weight %
60-70%
4-8% in water
4. FIRST AID MEASURES
Description of First Aid Measures
General Advice
Move affected individual away from the area of exposure.
Eye Contact
Rinse thoroughly with water for at least 15 minutes, holding the eyelid open to
be sure the material is washed out. Remove contact lenses if present after the
first 5 minutes of flushing if easy to do. Continue flushing. Get medical attention
if irritation persists.
Skin Contact
Wash thoroughly with soap and water. If skin irritation develops, seek medical
attention.
Inhalation
Move to area with fresh air. Seek medical attention if experiencing respiratory
effects.
Ingestion
If swallowed, seek medical attention.
Notes to Physician
This kit contains a few irritants including one sulfur compound (DTT) that smells
bad and may present some hazard to persons with underlying respiratory
problems and certain allergies.
Protection of First-Aiders
Wear gloves and take precautions to avoid direct contact with kit materials.
Most Important
Symptoms and Effects,
Both Acute and Delayed
Indication of immediate
medical attention and
Special Treatment Needed
May cause eye skin and respiratory irritation. May be harmful if swallowed
causing stomach irritation and nervous system effects.
Seek medical attention if eye or skin irritation persists after flushing. Seek
immediate medical attention if contents of tube 4 are swallowed.
5. FIRE-FIGHTING MEASURES
Suitable Extinguishing Media
Special Hazards Arising from the
Substance or Mixture
Dry chemical, carbon dioxide, foam or water.
Special Protective Actions for FireFighters
Standard gear. Use self-contained breathing apparatus if
necessary/desired.
Combustion will produce oxides of carbon and sulfur.
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6. ACCIDENTAL RELEASE MEASURES
Personal Precautions
Environmental Precautions
Wear personal protective equipment (safety glasses, gloves, etc.). Avoid
direct skin and eye contact. Avoid breathing vapors.
Avoid release to the environment. Report spills and releases as required to
appropriate authorities.
Methods for Containment
Keep containers closed when not in use. Avoid dispersal of spilled material.
Methods for Cleaning Up
Wear personal protective equipment (safety glasses, gloves, etc.), wipe up,
and contain in closed containers.
Reference to Other Sections
Refer to Section 8 for protective clothing and Section 13 for disposal.
7. HANDLING AND STORAGE
Precautions for Safe Handling
Standard good lab practices, including use in a well-ventilated area, and
PPE (lab coats, safety glasses, and gloves).
Conditions for Safe Storage,
Including any
Incompatibilities
Specific end use(s):
Keep containers tightly closed at the recommended storage temperature.
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES.
8. EXPOSURE CONTROLS / PERSONAL PROTECTION
Control Parameters (Exposure Limits/Guidelines)
Chemical Name
Exposure Limit
10 mg/m3 (as mist) TWA ACGIH TLV
15 mg/m3 (total), 5 mg/m3 (respirable) TWA US OSHA PEL
Glycerol
10 mg/m3 TWA (Belgium, France, Spain, UK)
50 mg/m3 (inhalable) TWA, 100 mg/m3 (inhalable) STEL Germany
DL-Dithiothreitol (DTT)
None established
Exposure Controls:
Ventilation:
Personal Protective Equipment (PPE):
Eye/Face Protection
Skin and Body Protection
Respiratory Protection
Hygiene Measures
No special ventilation required for normal use.
Safety glasses
Latex or nitrile gloves; lab coat /apron
None required
Standard good laboratory practices
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9. PHYSICAL AND CHEMICAL PROPERTIES
Appearance
Odor
Odor Threshold
None except tube with
DTT has unpleasant
sulfur smell
Flash Point
Clear
pH
7.5 – 8.0
Not Applicable
Autoignition
Temperature
Boiling Point/Range
Not Applicable
Water Solubility
Soluble
VOC Content
No data available
Decomposition Temp
Oxidizing Properties
Not Applicable
Flammability Limits in
Air (Upper)
Some liquids; some
lyophilized
Varies with individual
Not Applicable
Not determined
Flammability Limits in
Air (Lower)
Physical State
Melting Point/Range
Not Applicable
Partition Coefficient
(n-octanol/water)
None
No data available
Explosion Properties
Specific Gravity
Not Applicable
Solubility
None
Evaporation Rate
1.0 – 1.2 @ 20°C
Vapor Pressure
No data available
Viscosity
No data available
Vapor Density
No data available
No data available
No data available
10. STABILITY AND REACTIVITY
Reactivity
Chemical Stability
Possibility of Hazardous Reactions:
Conditions to Avoid
Incompatible Materials:
Hazardous Decomposition Products
This product is not reactive under normal conditions of storage
and use
Stable under recommended storage conditions.
No data available
Avoid excessive heat and open flames.
Avoid contact with strong acids, bases, & oxidizers
Carbon and sulfur oxides
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11. TOXICOLOGICAL INFORMATION
Information on Toxicological Effects
Principle Routes of Exposure
Ingestion, inhalation, skin, and/or eye contact
Potential Health Effects:
Eyes
Skin
Inhalation
Ingestion
Chronic Effects
Aggravated Medical Conditions
Interactions with other Chemicals
May cause irritation
May cause irritation
May cause irritation
Ingestion of the contents of tube 4 may be harmful if swallowed
causing stomach irritation and nervous system effects.
None known
None known
None known
Toxicity Data:
Product Information
The kit as a whole is not toxic, but a few components may cause irritation
upon contact.
Component Acute Toxicity Data:
Chemical Name
LD50 Oral
LD50 Dermal
LC50 Inhalation
DL-Dithiothreitol (DTT)
400 mg/kg - rat
No data available
No data available
Glycerol 12,600 mg/kg - rat
>21,900 mg/kg - rat
>570 mg/m3 – rat for 1 hr
Other Toxicity Data:
Skin Corrosion/Irritation Components may irritate the skin. Not corrosive. DTT is a skin irritant.
Glycerol in only a slight irritant.
Eye Damage/Irritation Components may irritate the eyes on direct contact. DTT is an eye irritant.
Glycerol in only a slight irritant.
Respiratory Sensitization No component is a respiratory sensitizer.
Skin Sensitization No component is a skin sensitizer.
Specific Target Organ DTT is a respiratory irritant.
Toxicity
Carcinogenicity No specific data is available for product. No component is classified as a
carcinogen.
Reproductive/ Developmental No specific data is available for product. No components known to pose a
Toxicity reproductive hazard.
Germ Cell Mutagenicity No specific data is available for product. No component is classified as a
germ cell mutagen.
12. ECOLOGICAL INFORMATION
Ecotoxicity
No data available for product. None of the components are classified as
harmful to aquatic organisms.
Persistence and Degradability
No data available for product or most components.
Bioaccumulative Potential
No data available for product or most components.
Results of PBT and vPvB
Assessment
Other Adverse Effects
None Required
No data available for product or most components.
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13. DISPOSAL CONSIDERATIONS
Waste Disposal Method(s):
CHEMICAL WASTE: The collective components of this kit and the collective waste generated in using this kit
are NOT regulated as USA RCRA hazardous waste or as California-only toxic waste. However, it is ultimately
the responsibility of the waste generator (end user) to determine the correct disposal method for all waste in
accordance with their applicable regulations.
BIOLOGICAL WASTE: Under normal use of Risk Group 1 biological materials/agents with this product, the
waste is NOT regulated as biohazardous or medical waste. However, other Risk Group materials/agents (2-4)
are regulated. International, federal, state, and local regulations vary; and, adherence to the CDC/NIH
“Guidelines for Research Involving Recombinant DNA” (or similar regulations in other countries) do (may)
require waste treatment of all recombinant DNA.
Important: Dispose all waste in accordance with local, national, and/or international regulations.
Contaminated Packaging
US EPA Waste Number
See above
None
14. TRANSPORT INFORMATION
DOT:
Proper Shipping Name
Hazard Class
Subsidiary Class
UN-No
Packing Group
Description
Not regulated
N/A
N/A
N/A
N/A
N/A
IATA/ICAO:
UN-No
Proper Shipping Name
Hazard Class
Subsidiary Class
Packing Group
ERG Code
Description
Not regulated
N/A
N/A
N/A
N/A
N/A
N/A
15. REGULATORY INFORMATION
International Inventories:
TSCA
For research use only.
DSL
For research use only.
EINECS/ELINCS
For research use only.
ENCS
For research use only.
IECSC
For research use only.
KECL
For research use only.
PICCS
For research use only.
AICS
For research use only.
U.S.A Federal Regulations:

No chemicals in the amounts present are reportable under SARA 313.
SARA 313
Chemical
Name
CAS-No
Weight %
N/A
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SARA 313-Threshold Values



SARA 311/312 Hazard Categories
Acute Health Hazard Yes
Chronic Health Hazard No
Fire Hazard No
Sudden Release of
No
Pressure Hazard
Reactive Hazard No
None of the chemicals in this product are listed as hazardous substances,
Clean Water Act
priority pollutants, or toxic pollutants under the Clean Water Act.
CERCLA
This product is not subject to CERCLA release reporting, however, many
states and local authorities have more stringent reporting requirements.
Chemical
Name
Hazardous Substance
RQs
Extremely Hazardous Substance RQs
N/A
U.S.A State Regulations:

California Proposition 65
International Regulations
Canada WHMIS
This kit does not contain any chemicals known to the State of
California to cause cancer or adverse reproductive health effects.
Class D-2-B (irritant)
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16. OTHER INFORMATION
NFPA
HMIS® III
Acute Health = 1; Flammability = 0; Instability = 0
Acute Health = 1; Flammability = 0; Physical Hazard = 0
Full text of classification abbreviations and R and H phrases for Reference (See Sections 2 and 3):
Xi – Irritant
Xn Harmful
R22 Harmful if swallowed
R36/37/38 – Irritating to eyes, respiratory system and skin.
Acute Tox 4 – Acute Oral Toxicity Category 4
Skin Irrit 2 - Skin Irritation Category 2
Eye Irrit 2A - Eye Irritation Category 2
STOT SE 3 - Specific Target Organ Toxicity Category 3
H302 Harmful if swallowed
H315 Causes skin irritation
H319 Causes serious eye irritation
H335 May cause respiratory irritation
Disclaimer: The information provided on this SDS is correct to the best of our knowledge based on information and
belief available at the date of publication. The information contained herein is designed only as a guide for safe
handling, use, processing, storage, transportation, disposal, and release. It is not a warranty or quality specification. This
information relates only to the specific material designated and may not be valid for use in combination with any other
material or in any other process (unless specified in the text).
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