Water quality and phytoplankton assessment in three selected waterfalls of Kuching and Serian Districts, Sarawak JASMINA BINTI MAJIT This dissertation submitted in partial fulfillment of the requirements for the degree of Bachelor Science with Honours in Aquatic Resource Science and Management Faculty of Resource Science and Technology UNIVERSITI MALAYSIA SARAWAK 2008 DECLARATION No portion of the work referred to in this dissertation has been submitted in support of an application for another degree qualification of this or any other university or institution of higher learning. _____________________ JASMINA BINTI MAJIT Aquatic Resource Science and Management Programme Department of Aquatic Science Faculty of Resource Science and Technology Universiti Malaysia Sarawak ACKNOWLEDGEMENT First and foremost, I would like to thank GOD for the continuous blessing that I have received during those challenging and wonderful times that I have gone through from the starting until the end of this project. Secondly, I would like to express my deepest gratitude and sincere appreciation to my supervisor, Dr. Samsur Mohamad, co-supervisor Dr. Ruhana Hassan, and other lecturers for their advices, guidance, supports and building comments from the very beginning until the completion of this project. Million of thanks are extended to my seniors, Mr. Nasarudin Harith and Ms. Ailen May for their kind assistances and valuable insight regarding this project. Sincere thanks also go to the laboratory staff especially, Mr. Nazri Latip, Mr. Zaidi Ibrahim and Mr. Mustafa Kamal @ Harris and other aquatic’s laboratory staff for their help and companionship throughout this project. Not to forget, special thanks to the Headman and villagers of Kampung Rayang for their willingness and precious help during the completion of this project. My heartfelt appreciation goes to all my supportive friends; especially Fam Choo Chen, Nur Amalina, Andrew Ujang, Anuar, Siaw Li Ching, Vincent Dainsi, Siti Fairuz, Jocelyn Jonip, Christharina Saurin, Nur Ezzaidah, Emmy Goh Ling Ling and Mary Teh Chee Sing for their helping hand, companionship, understanding and willingness. Last but not least, my devoted appreciation and greatest thankfulness goes to my loving and caring family, especially my parents: Mr. Majit Bin Bubat and Mrs. Ziniba Hj. Bujang, brother and sisters, relatives and Ally Basyrah for their unconditional love, supports and prayers. i TABLE OF CONTENT ACKNOWLEDGEMENT Page i TABLE OF CONTENTS ii APPENDICES iv LIST OF ABREVIATION v LIST OF TABLES vi LIST OF FIGURES vii ABSTRACT/ABSTRAK viii CHAPTER I INTRODUCTION 1 CHAPTER II 2.1 LITERATURE REVIEW Physical Parameter 2.1.1 Temperature 2.1.2 pH 2.1.3 Turbidity 2.1.4 Total Suspended Solid 2.1.5 Total Dissolved Solid 2.1.6 Dissolved Oxygen 2.1.7 Biochemical Oxygen Demand Chemical Parameter 2.2.1 Chemical Oxygen Demand 2.2.2 Ammonia-nitrogen 2.2.3 Nitrate 2.2.4 Nitrite 2.2.5 Orthophosphate Biological Parameter 2.3.1 Chlorophyll a 2.3.2 Phytoplankton Water Quality Monitoring in Sarawak 4 2.2 2.3 2.4 CHAPTER III 3.1 3.2 MATERIALS AND METHODS Sampling Sites Methods 3.2.1 Field Sampling 3.2.2 Laboratory Analysis ii 5 5 6 6 6 7 7 8 8 8 9 9 10 10 11 12 15 16 CHAPTER IV 4.1 4.2 4.3 CHAPTER V 5.1 5.2 5.3 5.4 5.5 RESULTS Water Quality Parameter 4.1.1 Physical, chemical and biological parameter during dry season 4.1.2 Physical, chemical and biological parameter during wet season Phytoplankton composition Water Quality based on DOE Water Quality Index 19 23 28 31 5.7 DISCUSSION Physical, chemical and biological parameter during dry season Physical, chemical and biological parameter during wet season Nutrient Analysis Phytoplankton composition Correlation for phytoplankton composition with water quality parameter Relation between phytoplankton composition and chlorophyll a Water Quality based on DOE Water Quality Index CHAPTER VI CONCLUSION AND RECOMMENDATION 52 CHAPTER VII REFERENCES 54 5.6 APPENDICES iii 33 37 42 44 46 49 51 APPENDICES APPENDIX I Calculation APPENDIX II Comparison of water quality parameters at 3 waterfalls and Water Quality Index APPENDIX III Method APPENDIX IV Human activities that are potentially alter the natural water quality APPENDIX V Phytoplankton identification iv LIST OF ABREVIATION pH Potential of Hydrogen TSS Total Suspended Solid TDS Total Dissolved Solid DO Dissolved Oxygen BOD Biological Oxygen Demand NO3- Nitrate NO2- Nitrite NH3-N Ammonia nitrogen PO43- Orthophosphate COD Chemical Oxygen Demand Chl a Chlorophyll a mg/L Milligram per Liter µg/L Microgram per Liter µm Micrometer GPS Global Positioning System NTU Nephelometric Turbidity Units ºC Degree Celcius km Kilometer WQI Water Quality Index v LIST OF TABLES Table No. Page 1 GPS reading for Ranchan, Giam and Rayang waterfall 13 2 Significantly different water quality parameters during dry season 19 3 Significantly different water quality parameters during wet season 23 4 List of phytoplankton identified at Ranchan, Giam and Rayang waterfall 29 5 DOE Water Quality classification based on Water Quality Index 32 6 Water Quality status at Ranchan 32 7 Water Quality status at Giam waterfall 32 8 Water Quality status at Rayang waterfall 32 9 Correlation of phytoplankton composition with water quality parameter 48 vi LIST OF FIGURES Figure no. 1 Location of Ranchan, Giam and Rayang waterfall 2 Page 14 Distinct parameter values at Ranchan, Giam and Rayang; (a) Temperature and (b) NO3- 21 Distinct parameter values at Ranchan, Giam and Rayang; (a) PO43- and (b) Chl a 22 Different parameter values at Ranchan, Giam and Rayang waterfall; (a) Turbidity and (b) BOD 25 Different parameter values at Ranchan, Giam and Rayang waterfall; (a) pH and (b) NO3- 26 Different parameter values at Ranchan, Giam and Rayang waterfall; (a) NO2- and (b) NH3-N 27 7 Relation between Chl a, PO43- and NO3- 35 8 Relation between Turbidity and TSS 39 9 Different proportion of Nutrient at Ranchan, Giam and Rayang waterfall 43 10 Phytoplankton composition identified at 3 different waterfalls 45 11 Relation between Phytoplankton composition and chl a (biomass) 50 3 4 5 6 vii Water quality and phytoplankton assessment in three selected waterfalls of Kuching and Serian Districts, Sarawak Jasmina Majit Aquatic Resource Science and Management Faculty of Resource Science and Management Universiti Malaysia Sarawak ABSTRACT The study was carried out in dry season (Apr’ 07 – Sept ’07) and wet season (Oct’07 - March’08) to assess the water quality levels and phytoplankton composition in three waterfalls located at Ranchan (Serian), Giam (Padawan) and Rayang (Borneo Height). A total of three sampling stations from each waterfall were selected as the 15 water quality parameters were analyzed based on APHA and HACH standard method. Meanwhile, phytoplankton samples were collected using plankton net (20 µm) and identified under inverted microscope. The results of physical, chemical and biological parameters were compared based on dry and wet season of each waterfall. It shows that temperature was significantly different among the waterfall during dry season, where both Giam and Rayang marked highest as 28 °C. Nitrate concentration (mg/L) was highest at Giam (0.14) and lowest at Rayang (0.01). Orthophosphate concentration (mg/L) however, marked highest level at Ranchan (0.5) and lowest at Giam (0.1). In chlorophyll a analysis, highest concentration (µg/L) was obtained in Giam (1.5), whereas, Ranchan recorded the lowest value which is 0.5. During wet season, Biological Oxygen Demand was significantly different where Ranchan recorded the highest concentration (2.2mg/L) while Rayang recorded the lowest value (0.7 mg/L). Turbidity level was recorded to be the highest at Ranchan (7.8 NTU) and lowest at Rayang (0.2 NTU). Ammonia nitrogen concentration (mg/L) however, marked highest level at Rayang (0.054) and lowest at Ranchan (0.031). For phytoplankton composition, a total of 58 genera have been identified where Rayang illustrates the highest number of genera (49 genera). Based on the Water Quality Index, Ranchan, Giam and Rayang are classified as slightly polluted due to their BOD level. Key words: Water quality, waterfall, phytoplankton, Water Quality Index ABSTRAK Kajian ini telah dijalankan pada musim panas (Apr’ 07 – Sept ’07) dan musim hujan (Okt’07 - Mac’08) untuk menilai kualiti air dan komposisi fitoplankton di tiga air terjun iaitu Ranchan (Serian), Giam (Padawan) dan Rayang (Borneo Height). Tiga stesen kajian untuk setiap air terjun telah dipilih untuk mengkaji 15 parameter kualiti air yang dianalisa berdasarkan piawai APHA dan HACH. Sampel fitoplankton diambil menggunakan jaring plankton (20 µm) dan dikenal pasti menggunakan mikroskop (inverted microscope). Keputusan parameter fizikal, kimia dan biologi air dibandingkan mengikut musim panas dan hujan. Suhu menunjukkan perbezaan yang ketara pada musim kering di mana Giam dan Rayang menunjukkan suhu tertinggi iaitu 28°C. Nitrat menunjukkan bacaan tertinggi di Giam iaitu 0.14 mg/L dan terendah di Rayang (0.01 mg/L). Orthophosphat mencatatkan nilai tertinggi di Ranchan (0.5 mg/L) dan terendah di Giam (0.1 mg/L). Untuk analisis klorofil a, nilai tertinggi dicatatkan di Giam (1.5µg/L), manakala Ranchan mencatatkan nilai terendah (0.5 µg/L). Pada musim hujan,. Ranchan mencatatkan nilai yang tertinggi untuk permintaan biologi oksigen iaitu sebanyak 2.2 mg/L dan nilai yang terendah dicatatkan di Rayang (0.7 mg/L). Kekeruhan air di Ranchan mencatatkan nilai tertinggi (7.8 NTU) manakala Rayang mencatatkan nilai terendah (0.2 NTU). Nilai Ammonia Nitrogen (mg/L) yang tertinggi dicatatkan di Rayang (0.054) dan terendah di Ranchan (0.031). Bagi komposisi fitoplankton, sebanyak 58 genera telah di kenalpasti di mana Rayang mencatatkan jumlah genera yang terbanyak iaitu 49 genera. Keputusan daripada setiap air terjun dibandingkan dengan Index Kualiti Air (DOE) di mana Ranchan, Giam dan Rayang telah dikenalpasti sebagai sedikit tercemar berdasarkan nilai BOD. Kata Kunci: Kualiti Air, air terjun, fitoplankton, Index Kualiti Air viii CHAPTER I: INTRODUCTION Water, a great gift from God is a very complicated and vitally important substance. It covers two thirds of the Earth’s surface while the human body consisting 75 percent of this substance (Chin, 2006). Without water, living organisms could not survive because water plays an important role in living organisms as it important to maintain health state, apart from oxygen and other necessities of life. Water pollution occurs when the water body is adversely affected due to the addition of large amount of materials to the water (Kumar and Bohra, 2006). The term water pollution is referred to any excessive addition to water in the form of materials (or heat) that are harmful to humans, animals, and aquatic life, that also causes significant departures from the normal activities of various living communities in or near the water bodies (Trivedi and Raj, 1992). Waterfall is one of the famous destinations for the public and tourists during leisure time. As a recreational destination, waterfall typically developed with essential services and facilities including food outlets. While most waterfalls remain more or less in their natural state, many of the more popular ones have undergone some development which may include refreshment outlets of different kind and various accommodations (Hudson, 1998). As the largest state in Malaysia, Sarawak has numerous waterfalls that are popular among tourists and some are unexplored as they are lack of publicity and isolated in rural areas. There are many waterfalls found in Serian District. Apart from Ranchan waterfall, another waterfall 1 that is well known is Simuja waterfall. It is accessible by road which is located about 7km from the town of Serian. Besides Giam waterfall, some waterfalls that can be found in Kuching District including Santubong Falls, Bobak Falls, Skedu Falls, Ban Buan Kukout and Mabi waterfalls. In terms of isolated waterfall, another waterfall that can be found besides Rayang waterfall (Borneo Height) is Ba’an Gong waterfall which is a hidden waterfall located in Penrissen area (Kuching). To gain access to this waterfall, one has to track about half an hour of cocoa gardens, paddy farms and rubber garden. Water Quality can be defined as the state of the suitability of water based on their physical, chemical or biological characteristics to sustain various uses or processes (Meybeck et al., 1996). Besides, Prakash (2005) defined water quality as the physical, chemical and biological characteristics of water that indicates the condition of water bodies whether it is healthy or polluted. Moreover, the quality of water may be described in terms of the concentration and state (dissolved or particulate) of some or all of the organic and inorganic material present in the water, together with certain physical characteristics of the water (Meybeck et al., 1996). Water quality monitoring is very important which it can give updated data of water bodies determining the health of monitored area. From the crucial need of water in our ecosystem, it is evidently clear that water is one of the prime elements responsible for life in Earth. Not much water quality study had been done previously, especially in waterfall areas, have been conducted in Sarawak. It is important to assess the water quality in waterfall particularly in the area that water utilization from waterfall by the local community’s as daily water supply are high 2 and waterfalls that are opened as recreational destination. Besides that, the waterfall area that is located near the potential water-polluting activities such as agriculture and housing area needs to be examined. Realizing the importance of waterfalls study in Sarawak, and lack of them, the main objective of this study is to assess the water quality in three different waterfalls sites in Kuching and Serian Area. Apart from that, the assessment is aimed to compare water quality levels in dry and wet seasons. Also, this study is conducted to relate the water quality data of each site based on the main activities practices at the area such as recreational, local utilization for bathing and washing, human settlement and agriculture activities. As well, the study is conducted to list and identify the phytoplankton as bioindicator for the water quality at the selected waterfalls. This study also aims to provide baseline data on water quality status and phytoplankton composition of the selected waterfalls for future study and research. 3 CHAPTER 2: LITERATURE REVIEW Water is an essential component in daily life, but nowadays the condition of water is in the unhealthy level due to pollution that is mostly cause by human activities. Water pollution can be resulted from natural runoff, dissolved chemicals in water that percolates through the soil, and human sources such as; agriculture, mining, construction, industry, homes and businesses (Trivedi and Raj, 1992). However, there are only few publications on water quality study in Sarawak, particularly in waterfalls area. In order to determine the quality of the water body, different parameter which consist of physical, chemical and biological qualities were analyzed. Water quality parameters are one of the matters which can be measured to find out the quality of the water in a water body (Prakash, 2005). Several important parameters that are commonly used in monitoring and determining water quality including temperature, pH, current, turbidity, Total Suspended Solid (TSS), Total Dissolved Solid (TDS), Dissolved Oxygen (DO), Biological Oxygen Demand (BOD), Nitrate (NO3-N), Nitrite (NO2-N), Ammonia Nitrogen (NH3-N), Orthophosphate (PO43-), Chemical Oxygen Demand (COD) and Chlorophyll a (Chl a) (Meybeck et al., 1996 and USEPA, 1997). 4 2.1 Physical Parameter 2.1.1 Temperature Temperature is one of the most important parameters for aquatic environment because almost all the physical, chemical and biological properties are governed by it (Trivedi & Raj, 1992). Nearly all aquatic life depends on water temperature for their optimal health. As only a few aquatic organisms are warm-blooded, the metabolic rate of virtually all organisms and overall system productivity are strongly influenced by water temperature (Cushing & Allan, 2001) adding that different organisms give response in a different way to temperature. Water temperature is first affected by air temperature, followed by the mixture of surface and groundwater, degree of shading and stream size (Kalff, 2001). Furthermore, the rates of biological and chemical processes depend on temperature which may affect other parameter such as Dissolved Oxygen (USEPA, 1997). 2.1.2 pH “Potential of hydrogen” or commonly known as pH is a measure of the concentration of hydrogen ions in the water. This parameter will determine the acidity or alkalinity of the water. Neutral water (distilled) has a pH of 7, acidic less than 7 and alkaline has pH more than 7. The desirable pH for freshwater aquatic life is in the range 6.5 to 9 and 6.5 to 8.5 for marine aquatic life (Chin, 2006). It is important to monitor the pH of the water body because it is closely related to the aquatic organisms. Changes in acidity can be caused by atmospheric deposition (acid rain), weathering of surrounding rock, wastewater discharges and organic matters (USEPA, 1997). 5 2.1.3 Turbidity Turbidity is a measure of water clarity or the light scattering properties of water cause by presence of particles and microscopic biota in water column which is measured in Nephelometric Turbidity Units, NTU (Chehalis River Council, 2007). Increase in the turbidity of the water decreases the amount of light that penetrates the water column, which may alter the aquatic ecosystem. As a result, reduction in photosynthetic activity of phytoplankton, algae and macrophytes will occur. Consequently, primary productivity and dissolved oxygen declines (Hauer & Hill, 2006). 2.1.4 Total suspended solid (TSS) TSS are discrete particles in water that can be trapped by a filter. TSS can include wide variety of materials such as silt, decaying plant and animal matter, as well as industrial wastes and sewage (Murphy, 2007). High concentrations of suspended solid can affect the water quality and can cause many problems in stream health and aquatic life. TSS refers to the dry weight of the material that is removed from a measured volume of water by filtration through a standard filter (Ballance, 1996). High amount of suspended solid indicate that the water bodies are contaminated with particles which causes low clarity of water (Trivedi & Raj, 1992). 2.1.5 Total Dissolved Solid (TDS) The substances remaining after evaporation and drying of a water sample are termed as ‘residue’. Non-filterable residue corresponds to the total suspended solid and the filterable residue is the Total Dissolved Solid (TDS). 6 2.1.6 Dissolved Oxygen (DO) DO refers to the amount of molecular oxygen dissolved in water and is one of the most important parameters affecting the health of aquatic ecosystems (Chin, 2006). It is an important parameter because its content shows the health and ability of the stream to purify itself through biochemical process (Trivedi & Raj, 1992). Changes in DO concentrations can be early sign of changing conditions in water body and its measurement provides a good indication of water quality (Ballance, 1996). There are many factors affecting the DO level in water such as; water current and turbulence, biological activities such as respiration and decomposition, and physical factor such as temperature (USEPA, 1997). 2.1.7 Biochemical Oxygen Demand (BOD) BOD is a measure of the oxygen consumed by micro-organism such as bacteria whilst breaking down the organic matter and shows the amount of molecular oxygen required by bacteria to reduce the carbonaceous materials (Trivedi & Raj, 1992). There are several factors that might affect the rate of oxygen consumption in a stream including; temperature, pH, type of organic and inorganic material such as leaves, woody debris, dead plants and animals in water and the presence of certain kinds of microorganisms in the water (USEPA, 1997). Waste discharges that contain significant amounts of biodegradable organic matter have high BOD levels and consume significant amounts of oxygen from the receiving waters, thereby reducing the level of DO and producing adverse impacts on aquatic life (Chin, 2006). 7 2.2 Chemical Parameter 2.2.1 Chemical oxygen demand (COD) COD is a measure of the oxygen equivalent of the organic matter content of a sample that is subject to oxidation by a strong chemical oxidant such as potassium dichromate in sulphuric acid condition (Trivedi & Raj, 1992). It provides a measure of the oxygen equivalent of that portion of the organic matter in a water sample that is susceptible to oxidation under the conditions of the test. It may be defined as the amount of oxygen required by organic matter in a sample of water for its oxidation by a strong chemical oxidant (Ballance, 1996). 2.2.2 Ammonia nitrogen (NH3-) Nitrogen is an important element in water monitoring since biological reaction can only proceed in the presence of sufficient nitrogen. NH3- is nitrogen in the form of ammonium salts which is in the form of non-colour gas that can produce weak base when react with water. Higher concentrations occur in water polluted by sewage fertilizers, agricultural wastes or industrial wastes containing organic nitrogen, free ammonia or ammonium salts (Ballance, 1996). Freshly polluted system especially by sewage contamination shows higher concentration of NH3- which changes to nitrites and nitrate in an aerobic environment (Trivedi and Raj, 1992). 2.2.3 Nitrate (NO3-) NO3-, the highly oxidized form of nitrogen compounds, is commonly present on the surface and in ground waters, because it is the end product of the aerobic decomposition of organic nitrogenous matter (Chin, 2006). Unpolluted natural waters usually contain only a minute amount of NO3-. In surface water, NO3- is the nutrient taken up by plants and assimilated into 8 cell protein (Ballance, 1996). The natural level of NH3- or NO3- in surface water is typically low (less than 1 mg/L) and in the effluent of wastewater treatment plants, it can range up to 30 mg/L (USEPA, 1997). 2.2.4 Nitrite (NO2-) Other form of nitrogen is NO2- which is in unstable form and intermediate stage in the nitrogen cycle which is formed in water either by the oxidation of NH3- or by reduction of NO3- (Chin, 2006). Consequently, biochemical processes can cause a rapid change in the NO2- concentration in water samples (Kalff, 2001). 2.2.5 Orthophosphate (PO43-) Organically combined phosphorus and all the phosphates are first converted to PO43- (Ballance, 1996). Both phosphorus and nitrogen are essential nutrients for the aquatic plants and animals (Kalff, 2001). Small increment of the phosphorus in the water, under right condition set off a whole chain of undesirable events in a stream including accelerated plant growth, algae blooms, low dissolved oxygen, and the death of certain fish, invertebrates, and other aquatic animals (USEPA, 1997). 9 2.3 Biological Parameter 2.3.1 Chlorophyll a (chl a) Analysis of the photosynthesis chlorophyll pigment present in aquatic algae is an important biological measurement which is commonly used to assess the total biomass of algae present in water samples (Ballance, 1996). The analysis of chl a concentration is much easier and provides a reasonable estimate of algal biomass. 2.3.2 Phytoplankton The response of biological communities or the individual organisms can be monitored in a variety of ways to indicate effects on the ecosystem. The changed ecosystem usually been associated with the organisms under stress or that the ecosystem has become unbalanced (Chapman & Jackson, 1996). Phytoplankton can be defined as the photosynthesizing plankton consisting primarily of microscopic algae, such as diatom and dinoflagellates (Oxford, 2006). It is an important bioindicator because it forms the basis of food chain of all other forms of aquatic life, being the primary producers that will affect the whole system if being altered. Basically, there are eight major division of algae which are; Division Cyanophyta, Prochlorophyta, Chlorophyta, Chrysophyta, Rhodophyta, Pyrrophyta, Cryptophyta and Euglenophyta (Sze, 1986). Naturally, they are important in food chain, but they are harmful to the environment if they are in the excess amount. Dense growth or blooms of algae are usually induced by human activity which will cause eutrophication of water bodies. 10 2.4 Water Quality Monitoring in Sarawak River Water Quality Monitoring has been started by Natural Resource and Environment Board (NREB), Sarawak since 1998. As rapid population growth, land development along river basin, urbanization and industrialization have subjected the rivers in Sarawak to increasing stress, giving rise to water pollution thus the common water quality parameters measured are those related to water pollution due to land clearing, sewage discharge and industrial effluents. Water quality is determined by comparing with relevant standard which is the Interim National Water Quality Standards (INWQS) for Malaysia. The State Government has directed NREB to ensure that the water qualities in all rivers of Sarawak are maintained at least to Class IIB of INWQS of Malaysia (Sumok, 2001). 11 CHAPTER III: MATERIAL AND METHODS 3.1 Sampling sites Three sampling locations were selected namely Ranchan, Giam and Rayang waterfall. The description and general observations were documented as follow: Ranchan Ranchan waterfall is located approximately 68 km from Kota Samarahan which is under Serian district. Accessible by road, it is popular recreational area (many visitors) and well known among tourists. Popular activities that can be found at this waterfall are picnic, swimming and bathing. Besides that, this waterfall is shaded heavily by vegetation along the watercourses. Giam Giam waterfall which is located at Padawan (Kuching District) is approximately 60 km from Samarahan. Accessible by road, it is also well known among the visitors especially local people. However, it is not as popular as Ranchan waterfall and the visitors are not as many as at Ranchan waterfall. This waterfall is also utilized by local people for daily washing and bathing. This waterfall offered wider swimming and picnic area. Besides that, the waterfall channel also provides transportation for the local area using boat (sampan) to go to their crop growing area. The vegetation also occurs along the channel with least shading as the channel is wider along the watercourse. 12 Rayang This isolated waterfall located in area which is approximately 70km from Samarahan. Difficult to access, this waterfall is not visited by tourist or local people for recreational purposes. This waterfall however, is only used by local people where the housing area can be found along the water channel. The agriculture activities such as small poultry farm also practiced near the watercourse. The vegetation is not heavily shading the water channel as the channel side is mostly built with houses. Three sampling stations have been assessed at each site comprising different sections of waterfall which are upper, middle and lower section (Appendix III). Table 1 shows the reading of Global Positioning System (GPS) that was used to determine the actual coordinates of three sampling site namely Ranchan, Giam and Rayang (Figure 1). Table 1 GPS reading for Ranchan, Giam and Rayang waterfall Sampling Site Ranchan Giam N 01º08.595' N 01º18.970' E 110º34.982' E 110º16.32' 13 Rayang N 01º06.937' E110º19.727' 2 Padawan 1 Ranchan 2 Giam 3 Rayang 1 Borneo Height 3 Figure 1 The location of Ranchan, Giam and Rayang waterfalls 14
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