SCIENCE TEST This document contains the science portion of the ACT practice test that is available for free at http://www.act.org/aap/pdf/preparing.pdf. Explanations of answers (in red print) were added by TJ Leone, and are not part of the official ACT prep material. 35 Minutes—40 Questions DIRECTIONS: There are seven passages in this test. Each passage is followed by several questions. After reading a passage, choose the best answer to each question and fill in the corresponding oval on your answer document. You may refer to the passages as often as necessary. You are NOT permitted to use a calculator on this test. Passage I Earthquakes produce seismic waves that can travel long distances through Earth. Two types of seismic waves are p-waves and s-waves. P-waves typically travel 6−13 km/sec and s-waves typically travel 3.5−7.5 km/sec. Figure 1 shows how p-waves and s-waves move and are refracted (bent) as they travel through different layers of Earth’s interior. Figure 2 shows a seismograph (an instru- ment that detects seismic waves) recording of p-waves and s-waves from an earthquake. Figure 3 shows, in general, how long it takes p-waves and s-waves to travel given dis- tances along the surface from an earthquake focus (point of origin of seismic waves). Earth’s interior. Figure 2 shows a seismograph (an instrument that detects seismic waves) recording of p-waves and s-waves from an earthquake. Figure 3 shows, in general, how long it takes p-waves and s-waves to travel given distances along the surface from an earthquake focus (point of origin of seismic waves). Earthquakes produce seismic waves that can travel long distances through Earth. Two types of seismic waves are p-waves and s-waves. P-waves typically travel 6−13 km/sec and s-waves typically travel 3.5−7.5 km/sec. Figure 1 shows how p-waves and s-waves move and are 0° earthquake focus Key p-waves s-waves both p-waves and s-waves received at seismographs both p-waves and s-waves received at seismographs core outer id qu li solid inner core 103° 103° shadow zone: neither p-waves nor s-waves received at seismographs shadow zone: neither p-waves nor s-waves received at seismographs mantle crust 142° 142° only p-waves received at seismographs 4 Note: The figure is not to scale. Figure 1 GO ON TO THE NEXT PAGE. ACT-64E-PRACTICE 42 time earthquake starts at the focus first p-waves arrive at seismograph first s-waves arrive at seismograph Figure 2 22 ch seismograph from uake focus (min) 20 18 s-waves 16 14 12 10 p-waves 4 1 minute 2. According to Figure 1, when p-waves encounter the boundary between the mantle and the core, the p-waves most likely: F. stop and do not continue into the core. G. enter the core and are refracted. H. change to s-waves. J. change to a third type of seismic wave. 3. Based on Figure 3, for a given seismograph, the time elapsed between the arrival of the first p-waves and the arrival of the first s-waves from an earthquake focus focus Figure 2 2. According to Figure 1, when p boundary between the mantle p-waves most likely: F. stop and do not continue into G. enter the core and are refract H. change to s-waves. J. change to a third type of seis 22 time to reach seismograph from earthquake focus (min) 20 18 s-waves 16 14 12 10 3. Based on Figure 3, for a given elapsed between the arrival of th arrival of the first s-waves from 10,500 km away would most like A. less than 5 min. B. between 5 min and 7 min. C. between 8 min and 10 min. D. more than 10 min. p-waves 8 6 4 2 0 0 00 00 00 00 00 00 00 00 00 00 1,0 2,0 3,0 4,0 5,0 6,0 7,0 8,0 9,0 10,0 distance along Earth’s surface from earthquake focus to seismograph (km) Figure 3 4. Based on the information prov quake starts at the focus” in Fi which of the following points on F. , 0 km, 0 min G. 2,000 km, 5 min H. 5,000 km, 12 min J. 10,000 km, 20 min 1. Figure 1 shows that a seismograph located at a point 125° around Earth from an earthquake’s focus 5. According to Figure 2, which o would receive which type(s) of seismic waves, if either, from that earthquake? ments best describes the relative A. P-waves only p-waves to arrive at the seism B. S-waves only s-waves to arrive at the seismogr the first p-waves to arrive at the C. Both p-waves and s-waves 1. nor Figure 1 shows that a seismograph located at a point D. Neither p-waves s-waves A. smaller than the amplitude 125° around Earth from an earthquake’s focus would arrive at the seismograph. receive which type(s) of seismic waves, if either, from B. larger than the amplitude o that earthquake? Answer: D. arrive at the seismograph. A. P-waves only C. nonzero, and the same as the B. S-waves only s-waves to arrive at the seism In Figure 1, 0° is shown t the p-waves spot labeled “earthquake focus”. Between 103° C. aBoth and s-waves D. and zero, as is the amplitude o 142° on either side of D.the Neither earthquake are areas labeled “shadow zone: neither p-wavesfocus nor s-waves arrive at the seismograph. p-‐waves nor s-‐waves received at seismographs”. Since 125° is between 103° and 142°, it is in the shadow zone, so it receives neither p-‐waves nor s-‐waves. ACT-64E-PRACTICE 43 2. According to Figure 1, when p-waves encounter the boundary between the mantle and the core, the pwaves most likely: F. stop and do not continue into the core. G. enter the core and are refracted. H. change to s-waves. J. change to a third type of seismic wave. Answer: G. The boundary between the mantle and the core is the circle around the area labeled “liquid outer core.” According to the key, the p-waves are solid lines with arrows to indicate direction. The s-waves are dotted lines with arrows. The lines for s-waves stop GO ON TO T when they reach the core boundary. The p-waves go through the boundary and bend. In Passage I, we are told that “Figure 1 shows how p-waves and s-waves move and are refracted (bent) as they travel through different layers of Earth’s interior.” This tells us that “refracted” means “bent”. So the p-waves are refracted when they enter the core. 3. Based on Figure 3, for a given seismograph, the time elapsed between the arrival of the first p-waves and the arrival of the first s-waves from an earthquake focus 10,500 km away would most likely be: A. less than 5 min. B. between 5 min and 7 min. C. between 8 min and 10 min. D. more than 10 min. Answer: D. In Figure 3, the horizontal axis of the graph shows “distance along Earth’s surface from earthquake focus to seismograph (km)”. The vertical axis shows “time to reach seismograph from earthquake focus (min)”. So the time elapsed between the arrival of the first p-‐waves and the first s-‐waves at a given distance is the vertical difference between the lines at that distance. For example, at 2,000 km, it takes 7 minutes for the first s-‐waves to reach the seismograph, and 4 minutes for the first p-‐waves. The time elapsed is 7-‐4 = 3 minutes. At 3,000 km, the time elapsed is 10-‐6 = 4 minutes. Since 10,500 km is not on the graph, we have to guess. We see that the distance between the s-‐wave and p-‐wave curves (time elapsed) is getting bigger. At 9,000 km, the distance between the curves is about 22-‐11 = 11 minutes. At 10,000 km, we can’t tell exactly since the s-‐wave curve is already past 23 minutes, but it looks like the distance between the curves continues to grow. We expect that at 10,500 km the distance will be even greater, so time elapsed is probably greater than 11 minutes. This makes D the best answer. 4. Based on the information provided, the “time earthquake starts at the focus” in Figure 2 corresponds to which of the following points on Figure 3? F. 0km,0min G. 2,000 km, 5 min H. 5,000 km, 12 min. J. 10,000 km, 20 min Answer: F. We are looking for the point on Figure 3 that represents the “time earthquake starts at focus”. The seismograph is at the earthquake focus when it is 0 km from the focus. The time 0 min marks the start of the earthquake. So the “time earthquake starts at focus” is represented by the point 0 km, 0 min (bottom left corner) on Figure 3. 5. According to Figure 2, which of the following statements best describes the relative amplitudes of the first p-waves to arrive at the seismograph and the first s-waves to arrive at the seismograph? The amplitude of the first p-waves to arrive at the seismograph is: A. smaller than the amplitude of the first s-waves to arrive at the seismograph. B. larger than the amplitude of the first s-waves to arrive at the seismograph. C. nonzero, and the same as the amplitude of the first s-waves to arrive at the seismograph. D. zero, as is the amplitude of the first s-waves to arrive at the seismograph. Answer: A. The amplitude of a wave is half the distance between the bottom and the crest of the wave. In the illustration below, the amplitude of the top wave is smallest, and the amplitude of the bottom wave is largest: In Figure 2, the amplitude of the first p-‐waves to arrive at the seismograph is smaller than the amplitude of the first s-‐waves to arrive. Passage II Lake Agassiz existed between 11,700 and 9,500 years ago in North America (see Figure 1). The lake was formed when a large glacier dammed several rivers. Groundwater trapped in lake and glacial sediments provides information about the climate at the time the sediments were deposited. Figure 2 shows a cross section of the sediments (lake clay and glacial till) and bedrock in the area. Figure 3 shows the δ18Ο values of groundwater taken from samples of the top 40 m of sediment at 3 sites along the same cross section. δ18Ο is calculated from a ratio of 2 oxygen isotopes (18O and 16O) in the groundwater. Smaller δ18 O values indicate cooler average temperatures. Lake Agassiz existed between 11,700 and 9,500 years ago in North America (see Figure 1). The lake was formed when a large glacier dammed several rivers. Groundwater trapped in lake and glacial sediments provides information about the climate at the time the sediments were deposited. Figure 2 shows a cross section of the sediments (lake clay and glacial till) and bedrock in the area. Figure 3 shows the δ18O values of groundwater taken from samples of the top 40 m of sediment at 3 sites along the same cross section. sted between 11,700 and 9,500 years δ18O is calculated from a ratio of 2 oxygen isotopes (18O (see Figure 1).16The lake was formed Manitoba and O) in the groundwater. Smaller δ18O values indicate dammed several rivers. Groundwater cooler average temperatures. acial sediments provides information maximum he time the sediments were deposited. extent of ss section of the sediments (lake clay Lake edrock in the area. Figure 3 shows the Agassiz dwater taken from samples of the top Site 1 • 3 sites along the same cross section. Site 3 • 18 • m a ratio of 2 oxygen isotopes ( O • • North dwater. Smaller δ18O values indicate Grand Dakota atures. Forks 4 Hudson Bay maximum extent of Lake Agassiz Site 1 Site 3 Hudson North Bay Dakota •• • • • Winnipeg Site 2 Great Lakes Grand Forks Winnipeg Site 2 Great Lakes Figure 1 Figure 1 surface surface surface 250 200 Key sediment/rock lake clay glacial till bedrock 250 Key sediment/rock lake clay glacial till bedrock 150 Figure 2 ACT-64E-PRACTICE 44 0 S 200 S 150 Site 3 Site 2 surface Grand Forks, 200 North Dakota Site 2 Winnipeg, Manitoba Site 1 N elevation (m above sea level) Site 3 250 Grand Forks, North Dakota Site 1 Winnipeg, Manitoba N 0 0 Manitoba GO ON TO THE NEXT PAGE. 150 Figure 2 44 GO ON TO THE NEXT PAGE. Site 1 Site 2 Site 3 δ 18 O δ 18 O δ 18 O smaller larger –26 –22 –18 –14 (surface) 0 10 20 lake clay 30 40 10 30 glacial till 40 glacial till – 1$ !"___________________________ O/ O of standard water sample # 18 Note: δ 18 O = lake clay 20 18 O/16O of groundwater sample 16 4 smaller larger –26 –22 –18 –14 (surface) 0 depth (m) depth (m) smaller larger –26 –22 –18 –14 (surface) 0 depth (m) 4 10 20 lake clay 30 40 × 1,000 Figure 3 Figures adapted from V. H. Remenda, J. A. Cherry, and T. W. D. Edwards, “Isotopic Composition of Old Ground Water from Lake Agassiz: Implications for Late Pleistocene Climate.” ©1994 by the American Association for the Advancement of Science. 6. According to Figure 2, the lake clay deposit is thinnest 9. According to Figure 2, which of the following graphs elevation (m above sea level) elevation (m above sea level) elevation (m above sea level) elevation (m above sea level) 6. According Figure 2, the lake of the following cities sites?sea level, of at which oftothe following cities or clay sites?deposit is thinnest at which best represents the elevations, in morabove the top of the glacial till layer at Sites 1, 2, and 3 ? F. Winnipeg F. Winnipeg G. Site A. 225 C. 225 G. Site1 1 H. Site 2 H. Site 2Forks J. Grand 200 200 J. Grand Forks 175 175 Answer: F. 150 150 1 2 3 1 2 3 Site Site According tothe Figure 3, at Sites 1, 2, and 3, the smallest In 7.Figure 2 , k ey s hows t hat l ake c lay i s i ndicated w ith l ight g ray. T he v ertical axis δ 18O value of the groundwater in the lake clay was B. 225 D. 225 of the figure s labeled recorded at a idepth between:“elevation (m above sea level). This tells us, for example, that A. innipeg 0 m and 10 m. has lake clay between 230 m and 2200 the W site 40 m above sea level. 200 In other B. 10 m and 20 m. words, the lake clay is 240-‐230 = 10 m “thick” at the W innipeg s ite. L ooking at the C. 20 m and 30 m. 175 175 D. 30 m and 40 m. figure, we can see that the lake clay (light gray area) is thinnest at the Winnipeg site. 150 150 1 2 3 1 2 3 Site 7. According to Figure 3, at Sites 1, 2, and 3, the smallest δ18Ο value of the groundwater in the lake Site clay was recorded at a depth between: 10. Precipitation that falls at Sites 1, 2, and 3 soaks into A. 0 m and 10 m. the soil until it reaches the groundwater table about 8. B. According to Figure 2, as the thickness of the lake clay 3 m below the surface. Based on Figure 3, and assum10mand20m. deposit increases from Grand Forks to Site 3, the ing no alteration of the precipitation, the δ18O value of C. 20mand30m. thickness of the glacial till beneath it: present-day precipitation in the study area is closest to: D. 30mand40m. F. increases. F. −26. remains the same. G. −23. G. H. first increases and then decreases. H. −20. J. decreases. J. −15. Answer: C. ACT-64E-PRACTICE TO v THE NEXT PAGE. In Figure 3, the smallest δ18O values are farthest to the left GO and ON have alues between 45 18 -‐26 and -‐14. At Site 1, the smallest δ O value recorded is a little less than -‐25 at a depth of around 27 m. At Site 2, the smallest δ18O value recorded is around -‐25 at a depth of around 27 m or 28 m. At Site 3, the smallest δ18O value recorded is a little less than -‐25 at a depth of around 28 m or 29 m. So the answer is C. 8. According to Figure 2, as the thickness of the lake clay deposit increases from Grand Forks to Site 3, the thickness of the glacial till beneath it: 8 lake clay 20 30 40 O= !" F. G. H. J. depth (m) depth (m) 10 10 20 lake clay 30 glacial till 40 increases. remains the same. first increases and then decreases. decreases. 18 O/16O of groundwater sample ___________________________ Answer: J. 18 O/16O of standard water sample # – 1$ × 1,000 Figure 3In Figure 2, the key shows us that the glacial till is indicated with stripes. As we move left from Grand Forks to Site 3, we see that the area of the glacial till gets ry, and T. W. D. Edwards, “Isotopic Composition of Old Ground Water from Lake Agassiz: thinner. o the thickness of the glacial till decreases. by the American Association for theSAdvancement of Science. B. C. 225 200 175 150 1 2 3 Site D. 225 200 175 150 1 2 3 Site elevation (m above sea level) elevation (m above sea level) A. elevation (m above sea level) d 3, the smallest lake clay was 9. According to Figure 2, which of the following graphs best represents the elevations, in m above sea level, of the top of the glacial till layer at Sites 1, 2, and 3 ? elevation (m above sea level) posit is thinnest ? 225 200 175 150 1 2 3 Site 1 2 3 Site 225 200 175 150 10. Precipitation that falls at Sites 1, 2, and 3 soaks into Answer: the soilC. until it reaches the groundwater table about of the lake clay 3 m below the surface. Based on Figure 3,18and assumto Site 3, the ing no alteration of the precipitation, the δ O value of Notice that the word elevation appears italics present-day precipitation in the study area isin closest to:in this question. In previous questions F. −26.about Figure 2, we’ve been look at the thickness of different layers. G. −23.wrote the question wants to make sure that we pay attention to elevation Whoever H. −20. instead of thickness. J. −15. In Figure 2, the elevation of a layer is shown with numbers along the vertical axis TOsea THE NEXT PAGE. labeled “elevation GO (m ON above level)”. So, for example, the top of the glacial till is 45 230 m at Winnipeg and 200 m at Site 1. Looking at sites 1, 2, and 3, we see that the elevation of the top of the glacial till is highest at Site 2 and lowest at Site 3, so C is the only possible answer. 10. Precipitation that falls at Sites 1, 2, and 3 soaks into the soil until it reaches the groundwater table about 3 m below the surface. Based on Figure 3, and assuming no alteration of the precipitation, the δ18Ο value of present-day precipitation in the study area is closest to: F. −26. G. −23. H. −20. J. −15. 4 Passage III Some students tested their hypothesis that the presence of bubbles in cans of various liquids would affect the roll time (the time it took a can to roll, without slipping, down an incline between 2 fixed points; see Figure 1). Experiment 2 The students added 1 L of the f an empty can. They sealed the can aside. Fifteen minutes later they fou can before and immediately after Again they set the can aside. Two h the roll time of the can before and im ing it (Trial 5). The results are shown Answer: J. We are told that present-‐day precipitation m below the present-‐day fixedsoaks points to 3incline surface. Since the data was recorded fairly recently (not thousands of years ago), we assume that we can use the data recorded in Figure 3 for δ18O values at 3m below Table 2 the surface. Notice that the values on the vertical axes start at zero on the top left Roll tim corner and get bigger as they go down. At all three sites, the δ18O value at around 3m before shaking a can below the surface Passage is about III -‐15. Experiment 2 Trial (sec) angle of The students added 1 L of the f inclination 4 Some students tested their hypothesis that the pres4 They sealed 1.86 the can an empty can. Passage III ence of bubbles in cans of various liquids would affect the 5 1.75 they fou aside. Fifteen minutes later Some students tested their of1bubbles cans of slipping, various liquids would affect roll hypothesis time (the that timethe it presence took a can to roll, inwithout Figure can before and immediately after the roll time (the time itdown took an a can to roll, without2slipping, down see an incline 2 fixed points; see incline between fixed points; Figurebetween 1). Again they set the can aside. Two h Figure 1). the roll time of the can before and im ing it (Trial 5). The results are shown Identical 1.2 L aluminum cans were used in the first two Experiment 3 fixed points ofincline experiments. The angle of inclination the incline was The students added 1 L of the f 2.3° in all three experiments. an empty 2 L clear plastic bottle a Table 2 t When they rolled the bottle down formed. They shook the bottle, cau Roll min tim and set the bottle aside. Fifteen bles were still visible, but after 2 ho before shaking a can be seen. Trial (sec) angle of inclination 4 1.86 Adapted from David Kagan, “The Shaken 5 1.75 by The American Association of Physics T Figure 1 Experiment 1 Identical 1.2 L aluminum cans were used in the first two experiments. The angle of inclination of the Identical 1.2 L aluminum used inwater the first two Experiment 3 The students added 1 Lcans of a were liquid—tap containincline was 2.3° in all three experiments. experiments. The angle of inclination was ing no bubbles—to an empty can, sealed of thethe can,incline and found The students added 1 L of the f 2.3°roll in all three experiments. its time. Next, they added 1 L of the tap water to a an empty 2 L clear plastic bottle a Experiment 1 second empty can, sealed it, shook it, and immediately When they rolled the bottle down t The students added 1 Lfound of a liquid—tap water containing nothese bubbles—to an empty the can, its roll time. They repeated procedures usingcan, sealed formed. They shook the bottle, cau water containing many bubbles, and a empty carbonated and found its roll time.soapy Next, they added 1 L of the tap water to a second can, sealed it, shook it, and and set the bottle aside. Fifteen min beverage that repeated contained noprocedures bubbles and that tasted flat, immediately found its roll time. They these using soapy water containing many bles were still visible, but after 2 ho having lost most of its carbonation. results areflat, shown bubbles, and a carbonated beverage that contained no bubbles The and that tasted having lost of its bemost seen. 11. In Experiment 3, what is the mos in are Table 1. in Table 1. carbonation. The results shown dents used the plastic bottle rat can? Compared to an aluminum Adapted from David Kagan, “The Shaken A. American rolled more rapidly the by The Association of down Physics T Table 1 B. made bubbles in the liquid e C. contained a greater quantity D. had thicker walls and was le Roll time Experiment 1 The students added 1 before L of a liquid—tap watershaking containshaking after ing no bubbles—to an empty can, sealed the can, and Trial Liquid (sec) (sec) found its roll time. Next, they added 1 L of the tap water to a second can, sealed it, shook 1 empty tap water 1.75 it, and immediately 1.75 found time. They repeated 2 its roll soapy water 1.97these procedures 2.15 using soapy containing many 1.75 bubbles, and a carbonated 3 water flat-tasting 1.96 beverage that contained no bubbles and that tasted flat, beverage having lost most of its carbonation. The results are shown in Table 1. ACT-64E-PRACTICE Table 1 Roll time 12. Based on the results of Experim of the following trials, before sh age speeds of the cans the same? F. Trials 1 and 2 G. Trials 2 and 3 H. Trials 2 and 4 J. Trials 3 and 5 11. In Experiment 3, what is the mos dents used the plastic bottle rat can? Compared to an aluminum GO ON TO T A. rolled more rapidly down the 46 B. made bubbles in the liquid e C. contained a greater quantity D. had thicker walls and was le 4 Experiment 2 The students added 1 L of the flat-tasting beverage to ested their hypothesis that the presan empty can. They sealed the can, shook it, and set it ns of various liquids would affect the aside. Fifteen minutes later they found the roll time of the Experiment 2 took a can to roll, without slipping, can before and immediately it sealed (Trialthe 4).can, shook it, and set it students of the flat-tasting beverage to anafter emptyshaking can. They een 2 fixed points; seeThe Figure 1). added 1 LAgain they set the can aside. Two hours later they found aside. Fifteen minutes later they found the roll time of the can before and immediately after shaking it the roll time of the can before and immediately after shak(Trial 4). Again they set theit can aside. hours later found the roll ing (Trial 5). Two The results are they shown in Table 2. time of the can before and immediately after shaking it (Trial 5). The results are shown in Table 2. fixed points incline Table 2 Roll time can ngle of nclination Figure 1 Trial before shaking (sec) after shaking (sec) 4 5 1.86 1.75 1.96 1.93 Experiment 3 The students added 1 L of the flat-tasting beverage to an empty 2 L clear plastic bottle and sealed the bottle. num cans were used When in the they firstrolled two the bottle Experiment 3 incline, no bubbles formed. They shook the bottle, causing bubbles to down the gle of inclination of the incline was form, and set the bottle aside. Fifteen minutes later, bubbles were still visible, to but after 2 hours, no The students added 1 Lsome of the flat-tasting beverage ments. bubbles could be seen. an empty 2 L clear plastic bottle and sealed the bottle. When they rolled the bottle down the incline, no bubbles formed. They shook the bottle, bubblesAssociation to form, of Physics Teachers. Adapted from David Kagan, “The Shaken-Soda Syndrome.” ©2001causing by The American and set the bottle aside. Fifteen minutes later, some bubbles were still visible, but after 2 hours, no bubbles could 11. In Experiment 3, what is the most likely reason the students used the plastic bottle rather than an be seen. aluminum can? Compared to an aluminum can, the plastic bottle: A. rolled more rapidly down the incline. from David B. made bubblesAdapted in the liquid easier Kagan, to see. “The Shaken-Soda Syndrome.” ©2001 by The American Association of Physics Teachers. C. contained a greater quantity of liquid. D. had thicker walls and was less likely to break. B. ed 1 L of a liquid—tapAnswer: water containempty can, sealed the can, and found hey added 1 L of theWe tap are water to that a the plastic bottle is clear. We are also told that in Experiment 3, bubbles told ealed it, shook it, and immediately are visibleusing after 15 minutes, but not after 2 hours. There is no mention of seeing any hey repeated these procedures ng many bubbles, and a carbonated bubbles in the other experiments. ned no bubbles and that tasted flat, s carbonation. The results are shown 12. Based on the results11. of Experiments 1 and 2, in is which of thelikely following trials, In Experiment 3, what the most reason the before stu- shaking, were the dents used the plastic bottle rather than an aluminum average speeds of the cans the same? can? Compared to an aluminum can, the plastic bottle: F. Trials 1 and 2 A. rolled more rapidly down the incline. G. Trials 2 and 3 Table 1 B. made bubbles in the liquid easier to see. H. Trials 2 and 4 C. contained a greater quantity of liquid. J. Trials 3 and 5 D. had thicker walls and was less likely to break. Roll time r before shaking (sec) 1.75 1.97 1.75 Answer: J. after shaking 12. Based on the results of Experiments 1 and 2, in which of the following trials, before shaking, were the average speeds of the cans the same? Trials 3 and 5 both have speeds of 1.75 seconds before shaking. 1.75 F. Trials 1 and 2 2.15 G. Trials 2 and 3 13. In1.96 Experiment 2, a result shaking the can H.ofTrials 2 and 4 of flat-tasting beverage was that the: A. number of bubblesJ.in the beverage decreased. Trials 3 andimmediately 5 B. mass of the can of beverage increased. C. roll time of the can of beverage decreased. D. roll time of the can of beverage increased. GO ON TO THE NEXT PAGE. 46 (sec) Answer: D. In Experiment 2, the roll time of the can of flat-tasting beverage increased from 1.75 seconds before shaking to 1.96 seconds after shaking. 14. In Trial 5, is it likely that bubbles were present in large numbers immediately before the can was shaken? F. Yes; based on the results of Experiment 1, the bubbles produced in Trial 4 probably lasted for less than 15 min. G. Yes; based on the results of Experiment 1, the bubbles produced in Trial 4 probably lasted for more than 2 hr. H. No; based on the results of Experiment 3, the bubbles produced in Trial 4 probably lasted for less than 2 hr. I. No; based on the results of Experiment 3, the bubbles produced in Trial 4 probably lasted for more than 3 hr. Answer: H. In Experiment 2, we are told that Trial 5 occurred 2 hours after Trial 4. In Experiment 3, we are told that 2 hours after the plastic bottle was set aside, no bubbles could be seen. From this we can conclude that the presence of bubbles immediately before shaking in Trial 5 was unlikely. 15. Suppose that in Experiment 2, two hours after the completion of Trial 5, the students had measured the roll time of the can of liquid without first shaking the can. Based on the results of Trials 4 and 5, the roll time would most likely have been: A. less than 1.86 sec. B. between 1.86 sec and 1.93 sec. C. between 1.94 sec and 1.96 sec. D. greater than 1.96 sec. Answer: A. After Trial 4, the roll time decreased from 1.96 seconds immediately after shaking 1.75 after setting the can aside for 2 hours. So 2 hours after Trial 5, one would expect the roll time to decrease, probably back down to 1.75 seconds (the roll time for both Trial 3 and Trial 5) and certainly below 1.86 seconds. 16. Based on the results of Trials 3−5 and Experiment 3, if the students had added 1 L of the flat-tasting beverage to one of the empty aluminum cans, sealed the can, and shaken it, how long would it most likely have taken for the number of bubbles in the beverage to become too few to affect the roll time? F. Less than 5 min G. Between 5 min and 14 min H. Between 15 min and 2 hr J. Over 2 hr Answer: H. From Trial 4 in Experiment 2, we saw that 15 minutes was not enough to bring the roll time down to the baseline value of 1.75 seconds in Trial 3. We also saw in Experiment 3 4 4 that bubbles were still visible after 15 minutes. However, from Experiment 3 we also learned that there were no bubbles visible after 2 hours, and in Trial 5 of Experiment 2, we saw that after the can was set aside for 2 hours, roll time went back to the baseline Passage IV Figure 2 shows the average rat speed of 1.75 seconds. rate ofrate photosynthesis (as % of at rate 670 at nm) of photosynthesis (asrate % of 670 nm) various wavelengths as a percent o The chemical reactions associated with photosynthesis photosynthesis at 670 nm. Passage IV Passage IV Figure 2 shows the average rat can be summarized with the following chemical equation: various The chemical reactions associated with photosynthesis can be summarized with the following chemical 110 wavelengths as a percent o The chemical reactions associated with photosynthesis photosynthesis at 670 nm. equation: 6,CO 2 + 12,H 2O + energy → C 6H 12O 6 + 6,O 2 + 6,H 2O can be summarized with the following chemical equation: 100 110 90 6,CO2 + 12,H2O + energy → C6H12O6 + 6,O2 + 6,H2O 100 80 Table lists wavelength for visible light and 90 Table 1 lists wavelength ranges for1visible light and the ranges color frequently associated with each range. 70 the color frequently associated with each range. 80 60 Table 1 lists wavelength ranges for visible light and 70 the color frequently associated with each range. Table 1 50 60 40 Wavelength Table 1 50 Color (nm) 30 40 Wavelength Violet 380−430 20 Color (nm) Blue 430−500 30 10 Green 500−565 Violet 380−430 20 Yellow 565−585 0 Blue 430−500 Orange 585−630 10400 440 480 520 560 Green 500−565 Red 630−750 Yellow 565−585 wavelength ( 0 Orange 585−630 400 440 480 520 560 RedA. Campbell, 630−750 Table 1 adapted from Neil Jane B. Reece, and Lawrence Figure 2 ( wavelength G. Mitchell, Biology, 5th ed. ©1999 by Benjamin/Cummings. Table 1 adapted from Neil A. Campbell, Jane B. Reece, and Lawrence G. Mitchell, Biology ed.by ©1999 by Benjamin/Cummings. relative absorption of, 5th light chlorophyll a and chlorophyll b versus relative absorption relative absorption Figure 1 shows the light from 400 nm to 750 nm. Figure 1 shows the relative absorption of light by chlorophyll a and chlorophyll b versus the wavelength of light Figure from 400 nm to 750 1 shows the nm. relative absorption of light by chlorophyll a and chlorophyll b versus the wavelength of light from 400 nm to 750 nm. Key chlorophyll a Key chlorophyll b chlorophyll a 100 chlorophyll b 90 100 80 90 70 80 60 70 50 60 40 50 30 40 20 30 10 20 0 10400 450 500 550 600 650 700 750 wavelength (nm) 0 400 450 500 550 600 650 700 750 wavelength (nm) Figure 1 Figure 1 ACT-64E-PRACTICE Figure 2 1 andof2 adapted from Peter H. theFigures wavelength Susan E. Eichhorn, Biology of Plants, 4th lishers, Inc. Figures 1 and 2 adapted from Peter H. R Susan E. Eichhorn, Biology of Plants, 4th lishers, Inc. 17. Based on Table 1 and Figure 1, associated with the wavelength the greatest absorption by chloro 17. Based on Table 1 and Figure 1, associated A. Blue with the wavelength the B. greatest Green absorption by chloro C. Yellow A. Blue D. Green Red B. C. Yellow D. Red GO ON TO T Figures 1 and 2 adapted from Peter H. Susan E. Eichhorn, Biology of Plants, 4th lishers, Inc. Figure 1 shows the relative absorption of light by chlorophyll a and chlorophyll b versus the wavelength of light from 400 nm to 750 nm. Key chlorophyll a chlorophyll b 100 90 relative absorption 80 70 60 50 40 30 20 10 0 400 450 500 550 600 650 wavelength (nm) 700 Figure 1 750 4 17. Based on Table 1 and Figure 1, associated with the wavelength the greatest absorption by chloro A. Blue B. Green C. Yellow D. Red velength ranges for visible light and ssociated with each range. Table 1 olor Wavelength (nm) olet ue een low ange d 380−430 430−500 500−565 565−585 585−630 630−750 A. Campbell, Jane B. Reece, and Lawrence d. ©1999 by Benjamin/Cummings. the relative absorption of light by orophyll b versus the wavelength of 750 nm. Key chlorophyll a chlorophyll b rate of photosynthesis (as % of rate at 670 nm) Figure 2 shows the average rate of photosynthesis at of the average rate of Figure 2 shows the average rate of photosynthesis at various wavelengths as a percent ACT-64E-PRACTICE various wavelengths as a percent of the average rate of 48 ctions associated withphotosynthesis photosynthesisat 670 nm. photosynthesis at 670 nm. th the following chemical equation: 110 energy → C6H12O6 + 6,O2 + 6,H2O 100 90 80 70 60 50 40 30 20 10 0 400 440 480 520 560 600 640 wavelength (nm) 680 720 Figure 2 Figures 1 and 2 adapted from Peter H. Raven, Ray F. Evert, and Susan E. Eichhorn, Biology of Plants, 4th ed. ©1986 by Worth Publishers, Inc. GO ON TO T 17. Based on Table 1 and Figure 1, which color of light is associated with the wavelength of light that results in the greatest absorption by chlorophyll b ? A. Blue B. Green C. Yellow D. Red Answer: A. Figure 1 shows that the greatest absorption by chlorophyll b occurs at a wavelength between 475 nm and 500 nm. Table 1 shows that this falls in the wavelength range for Blue (430 nm to 500 nm). 18. In eukaryotic organisms, the chemical reactions associated with the chemical equation shown in the passage typically occur within which of the following structures? F. Chloroplasts G. Mitochondria H. Lysosomes J. Nuclei Answer: F. Since photosynthesis involves, chlorophyll, a logical guess is Chloroplasts. 19. In Figure 2, at which of the following wavelengths does the rate of photosynthesis exceed the rate of photosynthesis at 670 nm? A. 400 nm B. 430 nm C. 630 nm D. 700 nm Answer: B. In Figure 2, the vertical axis shows the rate of photosynthesis as a percentage of the rate the rate at 670 nm. The rate is exceeded at values greater than 100%. Of the possible answers, only 430 nm has a rate of more than 100% of the rate for 670 nm. 20. In the chemical equation shown in the passage, the carbon in CO2 becomes part of which of the following types of molecules? F. Fat G. Sugar H. Protein J. Nucleic acid Answer: G. For this question, it helps to know that plants produce glucose during photosynthesis. Glucose is a sugar. You don’t need to know the chemical formula for glucose. Look at the equation shown in the passage: 6 CO2 + 12 H2O + energy → C6H12O6 + 6 O2 + 6 H2O We can see that the carbon from CO2 goes into C6H12O6. Since O2 is oxygen and H2O is water, C6H12O6 must be a glucose, or sugar molecule. 21. Which of the following conclusions is best supported by Figures 1 and 2? The wavelength that results in the highest rate of photosynthesis also results in the: A. lowest relative absorption by chlorophyll a. B. lowest relative absorption by chlorophyll b. C. highest relative absorption by chlorophyll a. D. highest relative absorption by chlorophyll b. Answer: C. 4 Passage V Experiment 3 A solid plastic bead was plac Students performed the following experiments to sample of each of Liquids 1−10 from determine the density of common plastics. the bead stayed at the bottom, From Figure 2, we see that the wavelengths that result in the highest rates of If Table 3. If the bead rose, “R” was re photosynthesis are around 440 nm and 680 nm. These values correspond with the highest procedure was repeated for various p relative absorption for chlorophyll a in Figure 1. Experiment 1 Passage V A dryexperiments 100 mL graduated cylinder was placed on an Students performed the following to determine the density of common plastics. electronic balance and tared (the balance was reset to 0.000 g). H2O was added to the graduated cylinder until a Experiment 1 certain mass was obtained. Ethanol was added to the graduPlastic A dry 100 mL graduated cylinder wasuntil placed an electronic balance ated cylinder theonvolume of liquid was and 50.0tared mL.(the Thebalance was reset to density the liquid was until then acalculated. 0.000 g). H2O was added to the of graduated cylinder certain massThe wasprocedure obtained. EthanolPolybutylene was added to VLDPE with ethanol H 2liquid O the gradu- ated cylinderwas untilrepeated the volume of different liquid wasamounts 50.0 mL.of The densityand of the wasLDPE then (seewas Table 1). with different amounts of ethanol and H O (see Table 1). calculated. The procedure repeated 2 HDPE PA-11 PA-6 Polycarbonate Table 1 PVC Mass of Mass of Total H 2O ethanol mass Density Liquid (g) (g) (g) (g/mL) 1 2 3 4 5 0 10.24 19.79 35.42 49.96 39.67 32.43 25.23 12.47 0 39.67 42.67 45.02 47.89 49.96 0.793 0.853 0.900 0.958 0.999 Table 3 Li 1 2 3 4 5 R S S S S S S S R R S S S S S S R R S S S S S S R R R S S S S S R R R R S S S S Experiment 2 A known mass of potassium iodide (KI) was dissolved in a known mass of H2O. A dry 100 mL graduated cylinder was placed on the balance and tared. The solution was added to the graduated cylinder until the volume was 50.0 mL. The density of2 the liquid was then calculated. The procedure was repeated with Experiment different amounts of KI and H2O (see Table 2). A known mass of potassium iodide (KI) was dissolved in a known mass of H2O. A dry 100 mL graduated cylinder was placed on the balance and tared. The solution was added to the graduated cylinder until the volume was 50.0 mL. The density of the liquid was then calculated. The procedure was repeated with different amounts of KI and H2O (see Table 2). Table 2 Liquid Mass of H2O in solution (g) Mass of KI in solution (g) Mass of solution in graduated cylinder (g) Density (g/mL) 6 7 97.66 95.41 7.36 15.52 52.51 55.70 1.05 1.11 22. In Experiment 1, the density of be: F. less than 0.793 g/mL. G. 0.793 g/mL. H. 0.999 g/mL. J. greater than 0.999 g/mL. 23. Based on the results of Experime PA-11 is most likely: A. less than 0.793 g/mL. in a known mass of H2O. A dry 100 mL graduated cylinder was placed on the balance and tared. The solution was added to the graduated cylinder until the volume was 50.0 mL. The density of the liquid was then calculated. The procedure was repeated with different amounts of KI and H2O (see Table 2). 22. In Experiment 1, the density of be: F. less than 0.793 g/mL. G. 0.793 g/mL. H. 0.999 g/mL. J. greater than 0.999 g/mL. Table 2 Liquid Mass of H2O in solution (g) Mass of solution in graduated cylinder (g) Mass of KI in solution (g) Density (g/mL) 4 23. Based on the results of Experime 6 97.66 7.36 52.51 1.05 PA-11 is most likely: 7 95.41 15.52 55.70 1.11 A. less than 0.793 g/mL. 8 94.38 20.68 57.53 1.15 B. between 0.853 g/mL and 0.9 9 92.18 29.08 60.63 1.21 C. between 0.999 g/mL and 1.0 10 87.77 41.31 64.64 1.29 D. greater than 1.11 g/mL. Experiment 3 A solid plastic bead was placed at the bottom of a med the following experiments Experiment 3 to sample of each of Liquids 1−10 from Experiments 1 and 2. of common plastics. A solid plastic bead was ACT-64E-PRACTICE If the bead bottom, “S”ofwas recorded in Experiments 1 and GO ON TO T placed at thestayed bottomatof the a sample of each Liquids 1−10 from 3. If the rose, “R”inwas recorded Table 3. “R” The was 2. If the bead stayed at Table the bottom, “S”bead was recorded Table 3. If theinbead rose, 50 recorded in wasfor repeated various plastics. Table 3. The procedureprocedure was repeated variousfor plastics. raduated cylinder was placed on an nd tared (the balance was reset to ded to the graduated cylinder until a ined. Ethanol was added to the gradue volume of liquid was 50.0 mL. The was then calculated. The procedure fferent amounts of ethanol and H 2O Table 1 Mass of ethanol (g) 39.67 32.43 25.23 12.47 0 Table 3 Liquid Plastic 1 2 3 4 5 6 7 8 9 10 Polybutylene VLDPE LDPE HDPE PA-11 PA-6 Polycarbonate PVC R S S S S S S S R R S S S S S S R R S S S S S S R R R S S S S S R R R R S S S S R R R R R S S S R R R R R S S S R R R R R R S S R R R R R R R S R R R R R R R S Total mass Density Experiment 1, the density of ethanol was found to be: (g) 22. In (g/mL) F. less than 0.793 g/mL. 39.67 G. 0.793 0.793 g/mL. 42.67 H. 0.853 0.999 g/mL. 45.02 0.900 greater than 0.999 g/mL. 47.89 J. 0.958 49.96 0.999 Answer: G. In Experiment 1, only Liquid 1 is pure ethanol. The density of the Liquid 1 was found to be 0.793 g/mL, so this is the measure of pure ethanol that was taken. 23. Based on the results of Experiments 1−3, the density of PA-11 is most likely: f potassium iodide (KI) was dissolved A. less than 0.793 g/mL. 2O. A dry 100 mL graduated cylinder B. between alance and tared. The solution was 0.853 g/mL and 0.958 g/mL. C. between ted cylinder until the volume was 0.999 g/mL and 1.05 g/mL. of the liquid was then calculated. Thethan 1.11 g/mL. D. greater ed with different amounts of KI and Answer: C. Table 2 Mass of KI in solution (g) Mass of solution in graduated cylinder (g) Density (g/mL) 22. In Experiment 1, the density of ethanol was found to be: F. less than 0.793 g/mL. G. 0.793 g/mL. H. 0.999 g/mL. J. greater than 0.999 g/mL. 23. Based on the results of Experiments 1−3, the density of PA-11 sank in liquids 1 through 5 and rose in liquids 6-10. The density of the liquids increased from 1 to 10. The density of PA-11 has to be greater than the density of liquid 5, since it sank in liquid 5, and less than liquid 6, since it rose in liquid 6. So the density of PA-11 is between 0.999 g/mL (the density of liquid 5) and 1.05 g/mL (the density of liquid 6) 24. Suppose that a sixth KI/H2O solution had been measured in Experiment 2 and the mass of the solution in the graduated cylinder was 67.54 g. The density of this solution would most likely have been closest to which of the following? F. 1.25 g/mL G. 1.30 g/mL H. 1.35 g/mL J. 1.40 g/mL 4 Answer: H. 4 Looking at Table we see the mass of solution for liquid is 64.64 g,1soand a mass 24. Suppose that a2,sixth KI/Hthat 26. In10Experiments 2, theof students tared the grad 2 O solution had been mea67.54sured would be an increase of 3.10 g. Between liquids 9 and 10, density goes up about in Experiment 2 and the mass of the solution in ated cylinder in each trial so they could more easi graduated cylinder was 67.54 g. The in density of this cylinder determine: 0.02 the g/mL per gram of mass of solution graduated since mass goes from 60.63 solution would most likely have been closest to which F. mass The of the substances added to the graduate g to 64.64 g (about 4 g) when density goes from 1.21 g/mL to 1.29theg/mL. of the following? cylinder. relationship mass of solution in cylinder and density G. looks F. 1.25 between g/mL thelinear, densityso of we the can graduated cylinder. 1.30 g/mLhold as we go from 64.64 g to 67.54 g. So a rise H. when thegtotal volume of the added substances w guessG.that it will of 3.10 in mass H. cause 1.35 g/mL equal to 50.0 mL. should a rise of about 0.06 g/mL in density, and 1.29+0.06=1.35. J. 1.40 g/mL J. when all of the KI was dissolved in the H2O. 25. A plastic bead was tested as in Experiment 3 using Liquids 1−4. Which of the following is NOT a plausible set of results for the plastic? Liquid A. B. C. D. 1 2 3 4 R R S S R R S S R S R S R S R S Answer: B. 27. A student claimed that polycarbonate is more dens than PA-6. Do the results of Experiments 1−3 suppo his claim? A. No, because in Liquid 8, polycarbonate stayed the bottom and PA-6 rose. B. Yes, because in Liquid 8, polycarbonate stayed the bottom and PA-6 rose. C. No, because in Liquid 8, polycarbonate rose an PA-6 stayed at the bottom. D. Yes, because in Liquid 8, polycarbonate rose an PA-6 stayed at the bottom. Since liquids get more dense as we go from 1 to 4, it is not plausible that a bead would rise in the (lower density) liquids 1 and 2 and the sink in the (higher density) liquids 3 and 4. 26. In Experiments 1 and 2, the students tared the graduated cylinder in each trial so they could more easily determine: F. the mass of the substances added to the graduated cylinder. G. the density of the graduated cylinder. H. when the total volume of the added substances was equal to 50.0 mL. J. when all of the KI was dissolved in the H2O. Answer: F. The word tared is italicized and defined in the description of Experiment 1. This is a clue that it will be used in one of the questions (we saw the same thing with the word refracted in Passage I). The definition given for tared is “the balance was reset to 0.000 g)”. Why would the students reset the balance to 0 after the graduated cylinder was placed on it? Because they are interested in the weight of the liquid added to the cylinder, not the weight of the liquid plus the weight of the cylinder. 27. A student claimed that polycarbonate is more dense than PA-6. Do the results of Experiments 1−3 support his claim? A. No, because in Liquid 8, polycarbonate stayed at the bottom and PA-6 rose. B. Yes, because in Liquid 8, polycarbonate stayed at the bottom and PA-6 rose. C. No, because in Liquid 8, polycarbonate rose and PA-6 stayed at the bottom. D. Yes, because in Liquid 8, polycarbonate rose and PA-6 stayed at the bottom. Answer: B. 4 Passage VI Experiment From Table 3, we see that polycarbonate and PA-6 are both plastics used to make beads2 for Experiment 3. If polycarbonate sinks in a particular liquid and PA-6 rises in that Synergism occurs when 2 Bacteria break down sugars by fermentation. To study together liquid, then polycarbonate is more dense that PA-6. This is exactly what happens forto ferment a sugar by using 2 fermentation pathways, researchers performed 2 experispecies can use alone. To investiga ments using broth that contained either the sugar sucrose or liquid 8. ment 1 was repeated, except that diff the sugar lactose. One of the fermentation pathways prospecies were added to each large test duces CO2 gas and increases the acidity (lowers the pH) of Passage VI the solution. The other pathway produces acid but not CO2. Bacteria break down sugars by fermentation. To study 2 fermentation pathways, researchers performed 2 experiments using broth that contained either the sugar sucrose or the sugar lactose. One of the fermentation pathways produces CO2 gas and increases the acidity (lowers the pH) of the solution. The other pathway produces acid but not CO2. Experiment 1 Species Sucrose broth was added to 5 large test tubes. Next, Experiment 1 added phenol red (a indicator that is red yellow pH < 7, that red isif yellow if pH < 7, red Sucrose broth was added to 5 large testpH tubes. Next, phenol (a pHif indicator pH ≥ 7) was added to each large test tube. A Durham tube A and B if pH ≥ 7) was added to(aeach large testtube) tube.was A Durham (a small tube) wastest placed, inverted, in small test placed,tube inverted, intest each large A and C each large test tube to collect Figure 1). tube toCO collect (see Figure 1). B and D 2 (see CO 2 C and D Table 2 Sucrose broth acid CO2 − + + + − + + + Durham tube broth (red) Figure 1 The large were capped, heated theOne of 4 bacterial The large test tubes were capped, heatedtest untiltubes the solutions were sterile, thenuntil cooled. cooled. One The of 4procedure bacterial species (Species A−D)solutions was added were to eachsterile, of 4 of then the large test tubes. was repeated using A−D) wastest added each of 4 of the large at a pH of 7) were lactose broth instead ofspecies sucrose(Species broth. The 10 large tubesto(all containing solutions tubes. then incubated at 37°Ctest for 48 hr. The procedure was repeated using lactose broth instead of sucrose broth. The 10 large test tubes (all containing solutions at a pH of 7) were then incubated at 37°C for 48 hr. 28. In Experiment 1, which of the mented lactose? The large test tubes and Durham tubes were examined. If acid was produced, the solution was yellow. If no F. Species B only acid was produced, the solution remained red. If CO2 was G. Species C only produced, a gas bubble was observed at the top of the H. Species B and Species D on Durham tube (see Table 1). J. Species C and Species D on 29. Suppose that in Experiment 2 test tubes. The procedure was repeated using lactose broth instead of sucrose broth. The 10 large test tubes (all containing solutions at a pH of 7) were then incubated at 37°C for 48 hr. 28. In Experiment 1, which of the mented lactose? The large test tubes and Durham tubes were examined. If acid was produced, the solution was yellow. If no F. Species B only acid was produced, the solution remained red. If CO2 was G. Species The large test tubes andproduced, Durham tubes were examined. acid was at produced, was yellow. If no C only a gas bubble was If observed the topthe ofsolution the H. Species B and Species D onl acid was produced, theDurham solution tube remained If 1). CO2 was produced, a gas bubble was observed at top (see red. Table J. theSpecies C and Species D onl of the Durham tube (see Table 1). 29. Suppose that in Experiment 2 Species C had been added to a l ing sucrose broth and to a larg Table 1 lactose broth. Which of the f likely depict the results? Sucrose broth Lactose broth Species Sucrose broth Lactose b added acid CO2 acid CO2 4 acid A − − − − B − − + + A. – C + + − − B. + D + − + − C. + − − − − ExperimentNone 2 D. – Synergism occurs when 2 bacterial species act own sugars by fermentation. To study together to ferment a sugar by using a pathway that neither Experiment 2 ays, researchers performed 2 experispecies can species use alone. To investigate ExperiACT-64E-PRACTICE Synergism occurs 2 bacterial act together to fermentsynergism, a sugar by using a pathway that neither contained either the sugar sucrose or whenment 1 was repeated, except that different pairs of bacterial can use investigate synergism, Experiment was repeated, different pairs of e of the fermentationspecies pathways pro-alone. To species were added to each large test1 tube (see Tableexcept 2). that52 creases the acidity (lowers the species pH) of were added to each large test tube (see Table 2). bacterial r pathway produces acid but not CO2. Table 2 as added to 5 large test tubes. Next, cator that is yellow if pH < 7, red if each large test tube. A Durham tube s placed, inverted, in each large test ee Figure 1). Durham tube broth (red) Figure 1 Species added A and B A and C B and D C and D Sucrose broth Lactose broth acid CO2 acid CO2 − + + + − + + + + − + + + − + + In these experiments, bacteria breaks down one of two sugars—sucrose or lactose—in two pathways. Both fermentation pathways increase acidity, but only one pathway produces CO2. Increasing acidity means lowering pH. The pH indicator phenol red is yellow if pH < 7 (acidity is high) or red if pH ≥ 7 (acidity is low). Solutions turn yellow if acid is produced. Otherwise, they remain red. Synergism occurs when two bacterial species work together to ferment a sugar in a way that neither can do alone. ubes were capped, heated until the 1, which of the bacterial species fermented lactose? 28. In Experiment e, then cooled. One of F. 4 bacterial Species B only ) was added to each of 4 of the large G. Species ure was repeated using lactose broth C only H. Species oth. The 10 large test tubes (all con- B and Species D only J. at Species pH of 7) were then incubated 37°C C and Species D only Answer: H. ubes and Durham tubes were examduced, the solution was yellow. If no e solution remained red. If CO2 was ble was observed at the top of the le 1). Table 1 ucrose broth Lactose broth 28. In Experiment 1, which of the bacterial species fermented lactose? F. Species B only G. Species C only H. Species B and Species D only J. Species C and Species D only 29. Suppose that in Experiment 2 both Species B and Species C had been added to a large test tube containing sucrose broth and to a large test tube containing lactose broth. Which of the following would most likely depict the results? CO2 acid – + + – + – + – C GO ON TO T of 4 of the large ing lactose broth st tubes (all conncubated at 37°C 28. In Experiment 1, which of the bacterial species fermented lactose? Species only For aF.sugar to beB fermented, it must produce acid alone, or acid and CO2. Under the G. Species C only heading “Lactose broth” in Table H. Species B and Species D only1, we see that acid and CO2 were produced by species J. acid Species C and D only B, and alone wasSpecies produced by species D. bes were examwas yellow. If no red. If CO2 was t the top of the 29.Suppose Suppose in Experiment both B Species B and 29. thatthat in Experiment 2 both 2Species and Species C had been added to a large test tube Species C had been added to a large test tube containcontaining sucrose broth and to a large test tube containing lactose ing sucrose broth and to a large test tube containing broth. Which of the following would most likely depict the results? lactose broth. Which of the following would most likely depict the results? se broth CO2 − + − − − Sucrose broth Lactose broth acid CO2 acid CO2 – + + – – + + – + – + – + – + – A. B. C. D. Answer: C. 52 GO ON TO THE NEXT PAGE. Comparing Table 1 and Table 2, we see that when two species are combined, they perform the fermentation they can do on their own plus any synergistic fermentation. For example, from Table 1 we see that species C ferments sucrose by producing both acid and CO2, and species D ferments lactose by producing both acid and CO2. In Table 2, we see that together they ferment both sucrose and lactose by producing both acid and CO2. There is a similar relationship between B and C, so we expect a similar result. 30. Suppose a scientist isolates a bacterial species that is 1 of the 4 species used in Experiment 1. She adds the species to sucrose broth and observes that neither acid nor CO2 is produced. She then adds the species to lactose broth and observes that both acid and CO2 are produced. Based on the results of Experiment 1, the species is most likely: F. Species A. G. Species B. H. Species C. J. Species D. Answer: G. From Table 1, we see that there are three species that produce neither acid nor CO2 in a sucrose broth when they are alone in the broth: species A, species B and species D. Of these, only species B produces both acid and CO2 in a lactose broth. 31. What is the evidence from Experiments 1 and 2 that Species C and Species D acted synergistically in Experiment 2? A. No acid was produced when each species was alone in the sucrose broth, but acid was produced when the 2 species were together in the sucrose broth. B. No acid was produced when each species was alone in the lactose broth, but acid was produced when the 2 species were together in the sucrose broth. C. No CO2 was produced when each species was alone in the sucrose broth, but CO2 was produced when the 2 species were together in the sucrose broth. D. No CO2 was produced when each species was alone in the lactose broth, but CO2 was produced when the 2 species were together in the lactose broth. Answer: D. Once again, we see an italicized word from a passage (synergism) being used in a question. From the description in Experiment 2, “synergism occurs when 2 bacterial species act together to ferment a sugar by using a pathway that neither species can use alone.” So, for evidence of synergism, we would need to see evidence of a pathway (production of acid or production of acid and CO2) that occurs when C and D are together (Table 2) that we don’t see when they are separate (Table 1). What we see in Table 1 is that species C does not ferment lactose and species D only produces acid in the lactose broth. However, when they are added together as shown in Table 2, they produce both acid and CO2 in the lactose broth. 4 terial species that is 32. Which 32. Which of the following best the of the following figures bestfigures illustrates theillustrates results of Experiment 1 for Species D in the sucrose ment 1. She adds the broth? results of Experiment 1 for Species D in the sucrose rves that neither acid broth? ds the species to lac- acid and CO2 are proF. H. Experiment 1, the Durham tube Durham tube broth (red) broth (yellow) gas bubble J. G. riments 1 and 2 that d synergistically in Durham tube Durham tube n each species was ut acid was produced ether in the sucrose broth (yellow) broth (red) gas bubble n each species was 33. IsG.the hypothesis that Species A and Species C acted t acid was produced Answer: synergistically supported by the results of Experiether in the sucrose ment 2 ? broth, species produces but no CO2. n each species was In the sucrose A. Yes, because bothDacid and COacid 2 were produced ut CO2 was produced no CO is produced, from sucrose. then no gas bubble is formed. 2 ether in the sucrose B. Yes, because both acid and CO 2 were produced from lactose. n each species was C. No, because only acid, not CO 2 , was produced t CO2 was produced from both sucrose and lactose. gether in the lactose D. No, because neither acid nor CO 2 was produced from lactose. Acid turns the broth yellow. If 33. Is the hypothesis that Species A and Species C acted synergistically supported by the results of Experiment 2 ? A. Yes, because both acid and CO2 were produced from sucrose. B. Yes, because both acid and CO2 were produced from lactose. C. No, because only acid, not CO2, was produced from both sucrose and lactose. D. No, because neither acid nor CO2 was produced from lactose. Answer: D. 4 Species C produces both acid and CO2 in sucrose, so we would expect both to be produced in a mix of A and C, even without synergy. For evidence of synergy, we would need to see the production of acid or both acid and CO2 when both A and C are present, since neither species alone produces acid or CO2 in lactose. Passage VII 34. Which of the following stateme with the DNA Hypothesis? The Passage VII In the 1940s, scientists thought all genetic material generally increase from cell ty In the 1940s, scientistswas thought all genetic material was contained in structures called and of: contained in structures called chromosomes and that chromosomes number had been foundofonly in (not the in nucleus of a cell that chromosomes had chromosomes been found only in the nucleus a cell the cytoplasm): F. amino acids in the nucleus in (not in the cytoplasm): to cell type. G. amino acids in the cytoplas type to cell type. H. chromosomes in the nucleu cytoplasm type to cell type. J. chromosomes in the cytopla type to cell type. nucleus 35. By referring to the observatio exclusively in the nucleus whi throughout the cell, the scientis Hypothesis implies that genes a because which of the following the nucleus? A. Amino acids Chromosomes composed proteins of 2 types molecules, pro-acid (DNA). B.Proteins Proteins Chromosomes are composed of 2 typesare of molecules, andofdeoxyribonucleic teins and deoxyribonucleic acid (DNA). Proteins are comC. Gametes are com- posed of subunits called amino acids. DNA consists of chains of subunits called nucleotides. The posed of subunits called amino acids. DNA consists of D. Chromosomes parts of chromosomes chains that areof responsible for the nucleotides. transmission The of genetic subunits called parts information of chromo- are called genes. Two scientists in the 1940s debate genes arefor made proteins or DNA. somes that whether are responsible the oftransmission of genetic information are called genes. 36. According to the passage, a sim and proteins is that both types of Protein Hypothesis Two scientists in the whether are Cells contain Genes are made only of proteins. Proteins make up 1940s 50% ordebate more of a cell’s genes dry weight. 20 found only in gametes. F. are made of proteins or DNA. G.proteins. are abundant in the cytoplasm different amino acids that can be arranged in a virtually infinite number of ways to make different H. contain 20 different amino a The number and arrangement of different amino acids within a protein form the codes that contain J. are composed of smaller sub hereditary information.Protein Hypothesis chromosomes are made only proteins. up believed 37. to According to the Protein Hypoth In contrast, only 4 different Genes nucleotides make up the of DNA found inProteins cells, andmake they are form 50% or more of a cell’s dry weight. Cells contain 20 differlowing observations provides t chains only in certain ratios. As a result, the number differentincombinations that DNA can carry is much ent amino acids that can be of arranged a virtually infinite that genes are NOT composed of smaller than the number that proteins can to carry. number of ways make different proteins. The number A. DNA is composed of only 4 and arrangement of different amino acids within a protein B. DNA is composed of smal form the codes that contain hereditary information. DNA Hypothesis proteins. Genes are made only of DNA. DNA is found exclusively in the cell’s nucleus, whereas proteins C. are found DNA is abundant in both In contrast, only 4 different nucleotides make up the throughout the nucleus and cytoplasm. Additionally, the amount of protein in a cell varies from cell type to cytoplasm. DNA found in cells, and they are believed to form chains cell type, even within the same animal. D. The concentration of DNA only in certain ratios. As a result, the number of different from cell to cell. combinations that DNA can carry is much smaller than the Though DNA is less abundant number than that proteins, proteins the canamount carry. is consistent from cell type to cell type within the same animal, except for the gametes (the reproductive cells). Gametes have half the amount38. of DNA as Mitochondria are organelles loc other cells in the body. Gametes also have half the typical number of chromosomes. Thus, the amount ofresponsible for energy tr that are DNA Hypothesis After the 1940s, it was observ contain their own genes. This o Genes are made only of DNA. DNA is found excluevidence stated in which hypothe sively in the cell’s nucleus, whereas proteins are found throughout the nucleus and cytoplasm. Additionally, the F. The DNA Hypothesis, beca amount of protein in a cell varies from cell type to cell of DNA, the observation wo type, even within the same animal. present outside the nucleus. DNA in a cell is correlated with the number of chromosomes in the cell. No such correlation is found for proteins. Chromosomes Proteins DNA amino acids nucleotides Genes are some part of chromosomes that transmit genetic information. Two scientists debate whether genes are made of proteins or DNA. 34. Which of the following statements is most consistent with the DNA Hypothesis? The amount of DNA will generally increase from cell type to cell type as the number of: F. amino acids in the nucleus increases from cell type to cell type. G. amino acids in the cytoplasm increases from cell type to cell type. H. chromosomes in the nucleus increases from cell type to cell type. J. chromosomes in the cytoplasm increases from cell type to cell type. Answer: H. DNA is made up of nucleotides, not amino acids, so we can eliminate choices F and G. At the time of this debate, chromosomes had only been found in the nucleus, not the cytoplasm. Since genes are part of chromosomes, we expect the number of genes to go up with the number of chromosomes. If the DNA Hypothesis were true, then the amount of DNA would rise with the number of genes, which rises with the number of chromosomes. 35. By referring to the observation that DNA is found exclusively in the nucleus while proteins are found throughout the cell, the scientist supporting the DNA Hypothesis implies that genes are made only of DNA because which of the following are also found only in the nucleus? A. Amino acids B. Proteins C. Gametes D. Chromosomes Answer: D. In the first sentence of the passage we are told that chromosomes had only been found in the nucleus at the time of the debate. This is relevant to the debate since the DNA argument is that DNA composes genes which are found in chromosomes. 36. According to the passage, a similarity between DNA and proteins is that both types of molecules: F. are found only in gametes. G. are abundant in the cytoplasm. H. contain 20 different amino acids. J. are composed of smaller subunits. Answer: J. DNA is composed of nucleotides and proteins are composed of amino acids. 37. According to the Protein Hypothesis, which of the following observations provides the strongest evidence that genes are NOT composed of DNA ? A. DNA is composed of only 4 types of nucleotides. B. DNA is composed of smaller subunits than are proteins. C. DNA is abundant in both the nucleus and the cytoplasm. D. The concentration of DNA is generally consistent from cell to cell. Answer: A. In the second paragraph of the Protein Hypothesis, we see “only 4 different nucleotides make up the DNA found in cells, and they are believed to form chains only in certain ratios. As a result, the number of different combinations that DNA can carry is much smaller that the number that proteins can carry.” The size of the subunits that compose DNA doesn’t support or weaken either hypothesis. Since proteins are also abundant in both the nucleus and the cytoplasm, C doesn’t help the Protein Hypothesis. Statement D supports the DNA Hypothesis. 38. Mitochondria are organelles located in the cytoplasm that are responsible for energy transformation in a cell. After the 1940s, it was observed that mitochondria contain their own genes. This observation contradicts evidence stated in which hypothesis? F. The DNA Hypothesis, because if genes are made of DNA, the observation would show that DNA is present outside the nucleus. G. The DNA Hypothesis, because if genes are made of DNA, the observation would show that DNA is present inside the nucleus. H. The Protein Hypothesis, because if genes are made of proteins, the observation would show that proteins are present outside the nucleus. J. The Protein Hypothesis, because if genes are made of proteins, the observation would show that proteins are present inside the nucleus. Answer: F. In the second sentence of the DNA Hypothesis we see the argument that “DNA is found exclusively in the cell’s nucleus.” Since mitochondria are in the cytoplasm, the presence of genes in the mitochondria means that genes are present outside the nucleus. Answer G makes no sense since the observation shows that DNA is present outside the nucleus, not inside it. Also, the observation of DNA inside the nucleus would not contradict the DNA Hypothesis. Answers H and J make no sense since the observation says nothing about the location of proteins. 39. The scientist who describes the DNA Hypothesis implies that the Protein Hypothesis is weakened by which of the following observations? A. For a given organism, the amount of protein in the gametes is half that found in other types of cells. B. For a given organism, the amount of protein in different types of cells is not the same. C. Protein molecules are composed of many subunits. D. Proteins are found only in the nucleus. Answer: B. At the end of the first paragraph in the DNA Hypothesis, the pro-DNA scientist says, “the amount of protein in a cell varies from cell type to cell type, even within the same animal.” In the second paragraph, he goes on to say that “the amount [of DNA] is consistent from cell type to cell type with the same animal.” This implies that the lack of consistency in amount of protein from cell to cell is important. Answer A gives an argument that was put forward about the amount of DNA in gametes (in the second paragraph of the DNA Hypothesis), not the amount of protein. For answer C, the fact that protein molecules are composed of many subunits was given as an observation that strengthens the Protein Hypothesis. 4 For answer D, both scientists accepted the observation that proteins are not found only in the nucleus. 40. Which of the following illustrations of a portion of a DNA molecule is consistent with the description o describes the DNA Hypothesis 40. Which of the following illustrations of a portion of a the passage? rotein Hypothesis isinweakened by DNA molecule is consistent with the description in the wing observations? passage? ganism, the amount of protein in the Key f that found in other types of cells. AA - amino acid ganism, the amount of protein in difN - nucleotide cells is not the same. ules are composed of many subunits. und only in the nucleus. F. AA AA N N G. H. J. Answer: J. N AA AA N AA AA AA AA N N N N END OF TEST 4 We are told in STOP! the passage DNA is composed of nucleotides. Amino acids compose DO that NOT RETURN TO ANY OTHER TEST. proteins. [See Note on page 56.] END OF TEST 4 STOP! DO NOT RETURN TO ANY OTHER TEST.
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