volume 10 Number 21982
N u c l e i c A c i d s Research
CoDection of published SS and 5.8S RNA sequences and their precursors
Volker A.Erdmann
Institut fur Biochemie, Fachbereich Chemie, Freie Universitat Berlin, Thielallee 69-73, 1000 Berlin
33 (Dahlem), GFR
The 1980 collection (1) of mature 5S and 5.8S RNA
sequences as well as those of their precursors are updated. This summary does not include those earlier publications in which the oligonucleotide composition, but
not the sequences of 5S RNAs. has been reported. For
this information the reader is referred to reference 2.
The possible structures and functions of prokaryotic 5S
and 5.8S RNAs are discussed in two other reviews (3,4).
I would also like to thank those colleagues who have
sent me their pre- or reprints on small ribosomal RNA
sequences in 1981.
© IRL Press Limited. 1 Falconberg Court, London W1V 6FG, U.K.
0306-1046/82/1002-T093 82.00/0
r93
30
40
SO
60
70
80
90
100
110
120
The tingle underlined sequence! *re tentative. The double underlined nucleotides occur in l e d th«n one mole per
nole 5S RNA. Nucleotide written directly under another nucleotide in the sequence indicate! that it may also be
found in this position. For abbreviation of organitma and literature referencea tee opposite page.
pUCgCUCGCGGCCgUACCCCGCUGCUaXACCUCACCCCAUCCCGAACUWGMcilCykMCCCCGUACCGCCGAUGGUAGWlHXMCUCUCCC^
pUCCCuttXCCCJGUAGCCCCCUCCUCCCACCUCACCCCAuraCGAACUCACMGUCAA
E.C.(a)
pUjjUGCUGjjgGAUgGCGAACAGCZCACACCCCWCCCAlACCCMUCCCAACinjAACCuCmiCACCCCCGAUCGUAGUCC^^
B.Q.
E.C. (b)
pCCUACUCCW;AUACXGGACCG(^M«CCa;UUCCCAlK:CCCAACACCCMGinjAAKgCUCCAGCGCCGAUCCUACTOCGG(XCAGCMCCCUGCAAGACU
puyUCGlK^CAUAKGAACACCu(JuUCCCCUaJCCCAUgCCGAACACGC^
B. S. (b)
B. S u .
OH
C
pUGCUU(£CGACWUAGCGAuuuGGACCCACCUwi|CUlJCCAuUCCGMCUCAGAAGu!y^
pUpCCUCJgCAUgCCGAACAGCUCACACCCCUUCCCAUCCCGAACACCCAACUUAACCUCuTJCAGCCCCGAW;CUACuT«KXXCCUUCCCCCUGjJGACAGUACGACJgCCCCA^C<^H
20
B.H
B.L.
10
pUCCUGCUGUCUAUCGCGCUAUCGAACCAOTCU^CCCCAUCCCGAACUCAGuTJGUCAAAWUACCUCCWXAACCAUACCUCCCGCGuACCCGCUCCCUAAAAUACCUC GACCCCAGCUC^
A. N.
1
EUBACTERIAL 5S RNA SEQUENCES
U)
3J
<B
oi
o'
c
13 - 17
133 1
- 1 16
6
Bacillus llchenlformis S 244
Bacillus megaterlum KM
Bacillus stearothermophilus 1439 FV
Bacillus stearothermophllua (strain not given)
Bacillus stearothenpophllus 799
Bacillus subtillB 168
Bacillus £
Closteridium pasteurianum ATCC 6013
Escherichia c o l i MRE 600
Escherichia c o l i CA265
B.L.
B.M.
B.S. (a)
B.S. (b)
B.S. (b)
B.Su.
B.Q.
C.P.
E.C. (a)
E.C. (b)
12
7
7, 9
11
10
9
8
6, 7, 9
72
Beneckea harveyi 392
B.B.
5
Reference Number
Anacystis Nidulans 1405/1 Katz/Allen
(Blue-green Alga)
RNA Sourse
A.N.
Abbreviation
Eubacterial 5S RNA Sequences
cS
CD
30
O.
Q.
3>
20
20
30
30
40
40
JO
JO
90
100
110
120
( B 1 ) pUCAGAGAACACCL'CUCAAUCG
p.B.Q.
i
i t *
i
»
i
i
»
m a t u r e 5 S RNA
Mature 5S RNA
m a t u r e 5S RNA
i
I
|UH
O H
i
I
i
t
I
t
i
I
i
t
I
i
t
I
|UH
i
i
| OH
t
The Blngl^ underlined «cqucnces are tentative. The double underlined nucleotidei occur in leoa than one mole per molo
5S RNA. Nuclcotideu written directly under another nucleotide in the sequence indicate* that it nay also be found in
this position. X indicates nucleotide unknown. For abbreviation of organisms and literature reference see opposite page
AAGCUUAAACCCAGCUCAAUGAGCUGGGUUUUUUGUuTJUUUC
t
CUUUUU
i
UUUWJn,,
t
pAUU, pUU and pU h a v e b e e n found a t t h e 5 ' e n d o f E . c o l i 5S RNA
i
i
GAUACUUCACCCAGUUCAUCxxxxxjaraXMiotM,.,,
I
AAUCAUACGUCAAAGACAGAUGCUGAGCUUUUmJCnu
p.E.C.
i
m a t u r e 5S RNA
AAUCAUACGGUCCCAUGCGAUGCUAAAGGCUUinJXQj^
pUGAGAGAACACUCUCAAUUUC
t
( B 2 ) pUGAGACAACACCUCUCAAUGC
it
i
m a t u r e 5S RNA
m a t u r e 5S RNA
p.B.Su.
p.B.Q.
( A 3 ) pUGAJACAACACCUCUCAAUCG
p.B.Q.
pUCAGAGAACACCUCUCAAUGC
(A2)
p.B.Q.
EUBACTERIAL 5S RNA PRECURSORS
M. C .
pUGAGACAACACCUCUCAAUGG
pUUGGUGGUAUAGCAUAGAGGUCACACCUGUUCCCAUGCCGAACACAGAAGUUAAGCUCUAU WCGeUGAAGAUAUUACUGAUGUGAGAAAAUAGCAAGCUGCCAGUUnu
OH
I . A.
T. T.
(A1)
pAAUCCCCCKCCuTJAGCCGCCUGCMMCCCGOTCCCAuTrcCCMCACCC^GUGAMCCaK^
PWyCCCCCGUGCtCAUAGCGGCGU^CWrcCGlTOCCCT^
P. F.
p.B.q.
pUCCuTCMGACMUAMCUUAUGCACCCACCuX^UeXCUUGCCGAAaJCACUACUCAAACCUAAUAGCGCCGAUGGTJAGUGUGGGOTCUCCCCAWnJW
pUCWJCUgUCACG ACUAGUG^CAUUGCAACACajGAireCCAUCCCGAACUCACAGGU^AAACCAUGC^UCGCCGAUCCUAGUCUKMOTn)^
P.
pUClttUGCCGGCCAUACCGCAGUGGUCCCACCUCAUCCC^UGCCGAACUCAGAACUGAAACGUUGUACCGCCGAUGAUGGUGUG^
80
P . V.
70
I
.
,
.
,
•
I
I
'
,
I
I
I 0H,
pCUUCACAUCCGCCAGCACCCCKCCAUUACACCCCCUAUCCAGCCCCAACCCGCAAGCCAAAGCCCCGAACCCCXCCAUCCUAGCUCGGGU AUCCCCCGGCAACACUUACGCAUCUCAACA
P
60
M.S.
L . V . (m) pUGUUGUMUGAUGGCAUTJMGGCCACACCUiaUCCCAUACCGMUU^
10
10
EUBACTERIAL 5S RNA SEQUENCES
^
3J
O.
CD
o_
Lactobacillus virldescens ATCC 12 706
Mycobacterium smegmatis SN2
Proteus vulgaris (strain not given)
Photobacter 8265
Pseudomonas fluorescens ATCC 13430
Thermua aquaticus ATCC 25104
Thermus thermophilus HB8
Mycoplasma caprlcolum ATCC 27 343
L.V.
M.S.
P.V.
P
P.F.
T.A.
T.T.
M.C.
25, 26
27
Bacillus subtilis 168
Escherichia coli 217
p .B .Su
p .E •C.
24
Bacillus
2
75
74
23
22
21
17
20
19
73
Reference Number
p .B • Q.(Ar B 2 )
Prokaryotic 5S RNA Precursors
Lactobacillus virideacens ATCC 12 706 (minor)
RNA Sources
L.V.(m)
Abbreviation
Eubacterial 5S RNA Sequences
o_
n
3}
9
>
o
<•>'
2
H.
T.
40
50
60
70
80
90
100
110
120
10
20
.
30
40
SO
70
80
90
100
Th« doubl* undarlln*d nuclootides occur in l e s s than one mol« per mole 5S RNA. For abbreviation of organisnfl
and litarature refaranca see opposite page.
60
5 S RNA SEQUENCBS
p^cCGGGCACUACGGlKaaCGlKAMACACCCGAUCC^UC^CCU^
1
MITOCHONDRIA
110
120
PVAUUCUGGUGUCCUAGGCGUAGAGGMCCACACCMUCCAUCCCGMCUIWGUGGUUAMCUCUACUGCGOreACSAUACUGUAGGGSAGGUCCUGCG<WAAAAUAGCUCGACGCCAGGAy0H
pUAUUCUGGUGUCCUAGGCGUAWGGMCCACACCAAUCCAUCCOWCUUGGUGGUU^
JO
CHLOROPLAST 5 S RNA SEQUENCBS
3"
n
CD
CD
CD
VI
o'
o
a.
B.B.
Duckweed (Lemna minor)
Broad bean (Vicia faba)
28
28
28
Chloroplast 5S RNA Sequences
D.
Dwarf bean (Phaseolua vulgari3)
76
Reference Number
D.B.
Spinach (Spinacia oleracia)
28, 77
Abbreviation
S.
Tobacco (Nicotiana tabacum var. Bright Yellow 4)
Mitochondria 5S RNA Sequences
Wheat embryo (Triticum vulgare Vill.,
Triticum aestivum L. var. Thatcher)
29
T.
W.
o
>
o
a.
3J
CD
CD
0)
81
T.A.
H. C.
H.H.
t
i
i
i
i
•
i
i
i
i
i
i
i
i
i
I
i
i
i
i
i
i
100 ^
>
110
The doubls underlined nucleotldes occur in less than one mole per mole 5S RNA. For abbreviation of oryanlama
and literature reference see opposte page.
GCCUCGCMGAGGGGCCAAGUGUGAGCC(^G(aGCGCMUCGGUAGWCACGCGWGUCCGUCCCGa]GGMCCCGAGACCGCUCCGUUACMCGGCUC^MCUAGU
B.M. 5S RNA lequence has the following 108 nucleotide Insertion between positions 108 and 109 :
pGGCAACGGUCAUAG^AGGGAAACACCAGAUCCCAUUCCGMbcGACGGUUAAGCCUGCUGCGUAUUGCGUU^UACUGU^
•
1
10
20
30
40
50
60
70
80
90
pUUAAGGCGGCCAUAKGGUGGGGUUACUCCCGUACCCAUCCCGAACACGGMGAUAAGCCCGCCIJGCGUUCCGGUCAGUACUGGMUGC^
tpUUAAGGCGGCCACAGCGGCG^CGACUCC'C(UMCCCAUCCCGAAMC^
ARCHAEBACTERIAL 5S RNA SEQUENCES
i
i
120
, ^
r
.8
o'
>
o
a.
</>
30
w
|S
Reference Number
18
78
79
80
Halobacterium cutirubrum N.R.C. 34001
Halococcus morrhuae ATCC 17082
Sulfolobua acidocaldarlus
Thermoplasma acldophilum (122-1B2 or 122-1B3)
H.. C .
U. M.
S. .A.
T,•
A.
Abbreviation
RNA Source
ArchaabacteriaL 5S RNA Sequences
u>
JO
a
srd.
o
c
o
•
t
JO
30
t
40
t
50 _
•
•
i
•
i
i
i
i
i
i
t
i
i
°
7
,
.
i
•»
,
,
i
'°
.
i
i
!°° .
i
,
!'° .
,
Un
'
,1M
i
i
i
•
i
t
I
•
i
I
.
M
i
i
•
i
•
i
p«;U<XCAUACCAU(>GCACUAAlK^C(»GAtKXAUCAGAACUCC<XACUlJMCCCUCCUUCC^
t
i
i
i
i
i
i
i
t
i
i
t
i
i
Ry-
pACAUACGACCAUACCCACUGGAAAACUC&JGAUCCCGUCCGCUCUCCCAUAGAUAAGCCAGUGAGGGCCA^CU
PGCCUACCCCCAUAC<>CCCUGAA<>CMCC<^UCUCClK^MUClK-CAArcUAA(X-iGGGUC^
Re.
M.F.S.p(kuU/i£GGC^ACWCCCI^aCGCCC<_A^^
N. C.
•
i
•
•
•
t
•
i
•
>
i
i
i
i
i
i
t
f*f*
i
Single underlined sequences are tentative. Double underlined nucleotidts or 5 1 phosphate* occur in less than one
n o W per mole 5S RHA. ? underneath the 3' terminal U of the D.B. sequence indicates that it has not clearly been
Identified as uridine. For abbroviations of organisas and literature references see opposite page.
R.T.
i
p^a)AC(:ACMUACWUCCU(ilAUAUACC«UUCUCCUC<!x^UCACCaAACUCAACCAGCAUACGCCUCCGU-AC^
"pGUCU ACGAC W U A C WCGUUGAAAKACCOHnJCUCWCCGAUC A C C G A A G U U A A G C M C G U G G G C C J ^
i
M.F.O.pGCUUACGGCCACACCMCCUGAG(_MGCCCGAUCUCGUCUGAUCUCGGMGC(^GCAGGGUUGGGCCUGG^
v
L. A .
i
H. L . pppCTCUACCOX^UACCACanKlkACGCreCCCAUCl)COTC.GAlX:iXXGMGCUAAGCAGGCUCG-<XCUCCUUACU
L
•
i
_.
.
.
__.___.
. _
_ _ _ . _ _ _
_
_ _ _
. .
.
_ _ _ . _ _ _ _ _ _ _ _ _ & _ _ _ _ . ,____r« * « _ _ & _ « _ ^«_p« • • • * • _ A # * « • # « _ * * t 1 _ « ^ 4 ^ 4 _ _ **r*fh
ll|ipi\uw<un\^UW^UnV^bWHillJUUIUWUiV(VWlK'liWIUVnUI-^UliWjJ1AWUUi-IA1AUUWUtnFUU>fU\i\mu
. pppCCCAACCACWUACCACCCUCAAUACAUC^ljmXW_^W^CCG^
P .
D. B.
D
D.
t. .
pAUGGAUlHSCUlJAUACClAJUAlKiAAMCUCCCCAUCCCAU-AGCACUG
i
C . R . I I
•
pAUGWUCGUUCAAACCUUCAAKCCCCUCCCCAUCCCAUC^ACUGGGAAGAUAAGCCU
i
i
i
i
i
i
i
•
•
,
•
i
i
•
'
i
i
' OH
•
H
U n
CU-U- U
yo^
CC I
OH
_ « 4 _ % * t _ «%_ • —
** W /MI
i
•
PCAGUACCA(XAUACUUGACUCAAAACACCAUAUCCCCUCCGAUUIK;UCAACUUAACCACCCACACCCUUACUUACUACUGACCUCACUCAUCACUCCGGAACCCUCA(:U<;CCCUACUCM^
pGCUGACGGCCAUACCGUGUCWAUGCACCI^UCUCUUCUGA^
C.R.I
C. F.
C. C.
cuuij
,
CUUU
,
to
pGCC AACGUCCAUACCAUGUUGAAUACACCGCWCUCGUCCGAUCACCGAAGUCAAG^
i
pGCAUACGACCAUAGGGUGUGGAAAACA&_GCUUCCCGUCCGCUCAGCCGUACAU^
pGGAUACGGCWUACUGCGCAGAAAGCACCKUUCCCAUCCGMCAGCGA^
10
C ( b ) EEPCCCUACrec^^CCACCCUC_UAACCCCCWlKHJCGlJCU«UciKX;GAACCUAACCAC^UCGGGCCUGGUUAGUACUUCM
•M •
N
,
c (a) EEPf^^cc^^iK^ccccucuAAcax^uciKrcrucuciMrarcc^
B
•
A. C.
A
1
EUKARYOTIC 5S RNA SEQUENCES
Chicken, embryo fibroplast culture
C
Duckweed
Drosophlla melanogaster F6 of KC
Dwarf bean (Phaseolus vulgaris)
C.P.
D.
D.M.
D.B.
Rye (Secale cereale c.v. Lovaszpatonai)
Rainbow trout (Salmo gairdneri, RTG-2)
Neurospora crassa
R.T.
Misgurnus fossilis (Somatic)
M.F.S.
Ry.
Misgurnus fossllls (Oocyte)
M.F.O.
Reptile (Iguana iguana)
82
Lytechinus variegatus
L.V.
Re.
87
Lingula anatina
L.A.
3 7 , 38
43
30, 31
42
87
41
86
39, 40
HeLa Cells
KB cells
H.L.
30, 31
36
28
35
85
34
84
33
32
83
30, 31
82
81
Reference Number
K.B.
(sea urchin)
Chlorella pyrenoldosa 211/8b
C.R.I, and II.
(Lemna minor)
Crlthldla fasclculata
Chlamydomonas relnhardli (CW 15)
C.F.
Crypthecodinlum cohnii
C.C.
(b)
Chicken (Gallus gallus), embryo fibroplast culture
roorl
C (a)
(Vicia faba)
Broad bean
Bombyx
B'.Mi
Aspergillus nidulans (strain paba A l , bi Al)
B.B.
Acantharooeba castellanl
A.N.
Source
A.C.
Abbreviation
Eukaryotic 5S RNA Sequences
^
8>
jo
2.
O
•
.
JO
i
•
*0
.
•
50
•
.
60
>
•
70
•
•
80
•
•
90
•
•
100
'
'
110
'
|>CCUCCGAUACCAUCAGCACUAAUCiACCC(^UCCAUCAC/LACUt;CCCAGUUAACXGUGCUUCCCCCACACUACUACUAKAUCCCUCACCCCCUGCCyUkCUCCUCCUCWCCACCU
pGGGUqCGAUCAUAC(^MCUMimCCGumCCAUCAGMCUCCGCAGUUMGCGUGCW
.
)2o
I
1
I
t
1
1
1
I
t
I
'
YTU
I
1
I
I
1
I
135
I
I
I
Single underlined aequeacea are tentative. Double underlined nucleotidea or 5' phoaphatea occur in leaa than one
• o l e per Dole 5S RNA. X in U.K. ia not certain; could be occupied by one or »ore unknown nucleotidea.
For abbreviation of organiama and literature referencea see oppoaite page.
at Che 3" end with CUU^ ( 6 0 1 ) , CUUUQH (201) and CUUUU^ (20X).
3S RNA ayntheaited by i a o l a t e d HeLa c e l l nuclei in v i t r o waa found to terminate
130
I
p.H.L.
l i t
I
Droaophlla pelanoRaater 5S RNA aequence plua at 3 ' end . . . CCUCCACAACUUUUUUnH
I
p.D.M.
EUKARYOTIC 5S RNA PRECURSORS
£p<OTUCCCCCCAUAW:UACCACAAAaaCCCTnJClKCCUCCCAUCMCUGW
125
jgpCCCUACCCCCACACCACCCUCAAACUCCCCCAUCUCCUCUGAUCUCCCAACCC^CCACCCUCGCCCClKXnmGM^
YSchPPpCUCUACGGCCAUACCUAGGCGAAAACACCAG'JUCCCGUCCGAUW^
XLS
1
•
1
ggpCUCUACGCCCAUACCACCCUCAACACCCCCCAUCUCCUCUGAW;UCCCAACCUMCCACGCUCCCCCCUWUUACUACUUCMUGCCAGACCUCCUCCCAAUACUCCCUGCUGUACCCUUy0H
T.t.
pCJjU^l'll.C^CCCAUACUAACCUGAAAACACCCCAUCCCAUUCCAACu^a^ACUUAACCCCCUUAACCCUCCCUUACUACUAACGUCGGCCACCCCUWKCAACU
W F
'
'
S.
Sp.
20
•
•
tO
EUKARYOTIC 5S RNA SEQUENCES
9J
3-
9
o*
JjJ"
Yeast (KluyveromyceB lactla)
Yeast (Plchla membranaefaclens)
Yeast (Schizosaccharomyces pombe, IFO No, 0345)
Yeast (Torulopsls utllla)
Y.K.L.
Y.P.M.
Y.Sch.
Y.T.U.
Drosophila melanogaster KcO
HeLa cells
p.D.H.
p.H.L.
Eukaryotic 5S RNA Precursors
Yeast (SaccharomyceB carlsbergenals)
Yeast (Saccharomyces carlsbergenBis)
Y.S.Ca. (a)
Yeast (Saccharomyces cerevlslae)
Xenopus mullerl (oocytes)
X.M.O.
Y.S.Ce.
Xenopus mullerl (somatic)
X.M.S.
Y.S.Ca. (b)
Xenopua Laevis (somatic from kidney)
Xenopus Laevis (oocytes)
Wheat embryo
W.E.
X.L.O.
Tetrahyemena thermophila
T.t.
X.L.S.
Turtle (Terrapene Carolina, TH-I line of hear cells)
Tu.
58
57
56
89
54
54
54, 55
54
53
52
52
42-44, 49-51
42-44, 49-51
46
45
44
30, 31
30, 31
Sunflower (Helianthua anuus)
Tomato (Lycoperslcum esculentum)
S.
88
Reference Number
Spinach (Spinacla oleracea L. var. 424)
Source
To.
Sp.
Abbreviation
Eukaryotic 5S RNA Sequences
SJ
3D
en
C
10
•
»
»
•
*
•
20
t
i
30
i
•
40
»
i
SO
i
•
60
i
•
70
•
*
80
i
i
90
t
100
*
i
i
,
.
,
I
t
I
I
I
t
I
I
I
I
»
T
UUCCAACGCACUUUCCCCCCCCAGCCCUUCCUCCUGCCCCUACCCCUCUCUCACUCUCCCUUou
UUCCAACCCACUUCCCCCCCCGCCUIICCUCCCCCCCCUACCCCUCUCUCACCCUCCCUU^
t
OH
Th. double underlinod nucleotide. occur in ltl> th.n on. aole per »ole 5.8S RNA. Nucl.otlde written directly under
th
indic
£°T,l
r f r i N.c.
! l nitquence
° " " "h«i
' " " b«en
" " t h fron
" " rDNA.
» » « 1 « b e f o u n d 1" ' h i ' PO»ition. m indicate, th.t the nucleotid.
U aethylated.
derived
R. T .
T.
UUCCAACCCACUUCCCCCCCCCCCUUCCUCCCCCGGCUACCCCUCUCUCACCCUCCCUQ
i
N. H.
.
IJGAACGUAUACUGCGCCCGAGGCCCCGGUAGAGCAUGUCUGCCUUAGUGCUGGGyU0l(
injCCAACCCACUUCCCCCCCCCCr.uUCCUCCCCCCCCUACCCCUCUCUGAGCCUCCCUU.,.
UUCAACCCACAUUCCGCUCCCCACUAUUCUCUCGAGCAUCCCUCUUCGACCCUUUUQH
-R-
N. C.
c
UGAAUGCAUACUGCUCCAGCUGACUUGUCAGUUGGAUAAUCUCCCUCAGGGACUA^
ISO
C. C .
UO
UUCGAACCCACUUCCCGCCCCCGGUUCCUCCCCGCCCUACGCCUGCCUCAGCCUCCCUU^. "
130
UGAACGCAAGUUGCGCUCUCQJGGUUUAACCCCCCGGGAGCACGIJUCGCUUGAGIJGCCGCU U Q,.
120
A.C.
110
C.
101
p£AACUCmjAACCCyCCAUCAClK:CGCUCGUCCCUCCAUCAAC^kCCCACC(XUAGCUGCGAGAAUUMUGyCAAUUCCAGCACACAUUCAUCAUCCACAC
pr^cuCUjjACCMj^WUCACUCCCCUCGUGCGUCGAUCAACMCCCAGCCCUACCVCCCACAACUAAUC^CAAUUgCACCACACAUUCAUCAUUCACAC
R •T .
-
pCGACuXUlMGCGCyGGAUCACu^CGCUCCUCCCUCCAUr^UVGAACCCACCCCUACCUCCCACAAUUAAUCyCAAUtJC^MACACAUUCAUCAUCCACAC
N. H.
T
pCCACUCuTJAGCCGJpjAUCACUCCCCUCGUCCCUCCAU^GAACCCACCCCUACCVCCCACMUUAAUCVCAAUUJCACCACACAUUCAlJCAUCCACAC
pAAACUUuX^CAACCCAUCWUlMXUUCu^CCAUCCAUCAAGAACCCACCCAAAUCCOAUACflUAAUCUrAAUUCCACAAUUCACUCAAUCAllCCAAUr.ll
H.L."V_
pAACUCUCAACAACGMUAUc'uUGGCUCUCG'GAUC^UGAAGGACGCAGC^UGCGAUACGUAG'uGUGMCUGfAGAAAUACGyGMCyAUCGAAUCCC
U.C.
•R-
pACAACUUUCAGCAGUUGAUUCCUUGGUUCAGACCUCGAUGAAGGGCACUGCGAAAGUGAAUGGCAUGUGMUGCAGGCAUCCGGGAAUUGAGAGCUUCU
c
C C.
PAACtCCUAACAACgGAVAUCUUGGUUCUCGCGAGGAUGAAGA^CGCAGCGAAAUGCGAUACGUAGUGUGAAUCGCAGGGAUCAGUGAAjJCATCGAAUCUT
pCAACUCUUACCWyGCAUCACUCCCCUCCUCCCUCCAUCAAGAACCCACCCCUAGCVGCCACAAinjAAUGliCAAUUgCACCACACAUUCAUCAUCCACAC
•c •
5•8S RNA SEQUENCES
•
C
11
EUKARVOTIC
§•
*
<J>
O
QL
o
62
63
64
Crypthecodlnium cohnli
Chlamydomonas reinhardil
HeLa cells
Mouse (MPC-11 cells)
Neurospora crassa
Novikoff hepatoma ascites cells
Rainbow trout (Salmo gairdneri, RTG-2)
Turtle (heart cells CCL 50)
c.c.
C.R.,
H.L.
M.
N.C.
N.H.
R.T.
T.
61
60
59, 60
85
84
59
Chicken (embryonic cells)
C.
81
Reference Number
Acanthamoeba castellanl
RNA Source
A.C.
Abbreviation
Eukaryotic 5.8S RNA Sequences
Z
3
a
CD
33
(A
d.
o'
CD
o_
SO
60
70
80
90
100
i
130
t
>
i
U0
i
130
,
i
•L •
X
UUCGAACCCACCUUCCCCCCCCCCCUUCCUCCCCCGCCUACGCCUCUCUCACCCUCCCUJoH
i
i
i
i
i
i
t
i
t
i
*
^
y
i
i
*
t
AACCCAUAUCCCACUCCAUCCUCuunuguacuuuasuunauuuunungugcUCCUUCCACUACAUAUCCUUCACCOJUCUA^
i
UUCAACCCACAUUCCCCCCCUUGGUAUUCCACCCGCCAUCCCUCUUUCACCCUCAUUDn,.
UUCCAACCCACCUUGCGGCCCCGCCUUCCUCCCCCCCCCACCCCUC UCUGAGCCUCCCUC J ^
The double underlined nucleotides occur in l e s s than one mole per Hole S.8S RNA. Nucleotide written d i r e c t l y under
another n u c l e o t i d e in the sequence indicates that i t may a l s o be found in this p o s i t i o n , m i n d i c a t e s that the nuclaotide
i s methylated. N.C. sequence has been derived from rDNA.* Nucleotides 1-123 correspond to «5.8S and n u c l e o t i d e s 152-181
to 2S RNA; n u c l e o t i d e s 124-151 are part of the precursor and reaoved during processing. For abbreviations of organisa*
and l i t e r a t u r e references see opposite paga.
D. H. *
Y.S.Ce.
•B •
X
GUCUUUGAACGCAAGUUGCGCCCGAGCCACUCGCCGAGGGCACGCCUGCCUGGGCGUCACGCnu
i
120
W. E .
i
CUCUUUCAACCCAAGUUCCCCCGAUCCCAUUACCUUCACCCCACCUCUCCCUGCCUCUCACAU^
110
V. F.
' i i
101
pAACUCUAACCMlWCAUCAClK^CUCAUCCCUCCAUCAACAACCCACCAAACUCUCCGUCAUCCUClKIAACUCC^MACACAUCAACAUCCACAUUUUC
p/UUlCUinjCAACAACCCAUCUCUUCCUUCUCCCAUCCAU(y^
Y. S . C e .
•M • *
X.L.
D
pCCACUCUUACCCCyCCAUCACUCCCCUCCUCCCUCGAUCAAGAACMACCCCUAGCyCCCACAAUUAGUC^CAAUUgCACCACACAUUCAUCAUCCACAC
pCCACUCUUACCCCj|CCAUCACUCCCCUCCUCCCUCCAUGAACAACCCACCGCUAGCJGCWGAAUUACUCyGA>mjjCAGWCACAUUCAUCAUCCACAC
X . B.
pJACACGACUCUCGGCAACGGAf AUCUCGGWCUCdCAUCG^UGAAGAACGUAGCGAMUGCt^kcUGGUGUGAAUrgCAGAAUCCCGCGAACCAUCGA
40
w. E.
30
pACAAUCAClKIWXICCAACCCATAUCUACCCUCUUCCAUCCAlX^ACAACCUAGCCAAAUCCCAUACUUGGUCUCAAUTjJCACAAUCCCCUCAACCAUCCA
20
5 . 8 S RNA SEQUENCES
V.P.
10
EUKARYOTIC
Wheat ambryo
Xenopus borealis (somatic)
Xenopus leavis (somatic)
Yeast (Saccharomyces cerevisiae A364A gal-1 ade-1 ade-2
ura-1 his-7 lys-2 try-1 (ATCC 22 244))
Drosophila melanogaater
W.E.
X.B.
X.L.
y.S'.Ce.
D.M.*
68
67
59, 66
66
46
65
Reference Number
Vlcla faba (broad bean)
RNA Source
V.F.
Abbreviation
Eukaryotic 5.8S RNA Sequences
JO
CD
d.
u>
o_
a
o'
Three d i f f e r e n t
p.X.L.
5 ' and 3 ' n u c l e o t i d e s e q u e n c e s i n p r e c u r s o r 5 . 8 s RNA were
i
i
t
i
i
i
underlined
Yeast
Yeast
p.Y.S.Ca.
p.Y.S.Ce.
Single
Xenopus l e a v i s
p.X.L.
(somatic)
RNA Source
sequences
are tentative.
(Saccharomyces c e r e v i s i a e
S-74) .
71
70
66,69
5 9
Reference Number
S288 a mal g a l - 2 )
(Saccharomyces c a r l s b e r q e n s i s ,
HeLa c e l l s
p.H.L.
Abbreviation
EUKARYOTIC 5 . 8 S RNA PRECURSORS
p . Y . S . C c . 4>UAUUAA and pAUAUUAA have been found a t t h e 5 ' end o f t h i s y e a s t S.8S RNA.
p . Y . S . C a . The f o l l o w i n g a d d i t i o n a l sequence h a s been found a t t h e 3 ' end: CCUUCyCAAACAUUCUGp
3'end:..CACCUCCAuCCCCCCCCCCCCCUCCCUCCCCCCoH
i
CCCCCCCCCCCACCCCUCACACCGCACCCCCCCUACCCCUGCCCACACCCAAAACCAAAACCCACCCACGCCUCCGCCACACCUCC . . .
deduced a s f o l l o w s :
From DNA s e q u e n c i n g data the a d d i t i o n a l
5'end:
S.8S RNA.
5 ' n u c l e o c i d e s a r e r e p o r t e d : pUCG ( 4 0 2 ) , pCC (202) and pG (AOZ).
pUCC i n s t e a d of pCC has a l s o been found a t Che 5 ' end of HeLa c e l l
p.H.L.
EUKARYOTIC 5 . 8 S RNA PRECURSORS
Nucleic Acids Research
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