Art. No. S6119
Version 1.0 E
Background
Specificity
According to the European Commission Directive 2002/86/EC, food
ingredients have to be declared. In case of meat, the specification
of the animal species has to be disclosed on the label.
In Germany the necessity of identifying meat components to
prevent potential misleading of consumers is based on §11 of the
German Food and Feed Law. If material from animal species, other
than the declared ones, are used as components, or if components
are used, whose absence is stated in the labeling, the respective
food should be rejected.
Tab. 1 specificity results of the ANIMAL ID Horse & Donkey IAAC
real-time PCR detection method (examples)
In order to enable the authorized food control agencies or quality
assurance of the food industry to supervise compliance with the
labeling requirements suitable detection methods must be available.
Method
®
After DNA extraction with SureFood PREP Basic the animal species
horse (Equus caballus) and donkey (Equus asinus) will be fast and
sure detected with the real-time PCR technology. Horse-DNA is
detected in the FAM channel and donkey-DNA is detected in the
Cy5 channel. In the VIC/HEX channel it is possible to detect animal
DNA in the sample as well as the amplification control in a sample
with no animal DNA inside (internal inhibition control).
Through the prepared limit of detection it is possible to distinguish
intentional
admixture
from
technological
unavoidable
contaminations.
Horse
(FAM
channel)
Donkey
(Cy5
channel)
IAAC
(VIC/HEX
channel)
Horse (Equus caballus)
2 % Horse in animal material
positive
negative
positive
positive
negative
positive
Donkey (Equus asinus)
negative
positive
positive
Beef (Bos taurus)
negative
negative
positive
Pork (Sus scrofa)
negative
negative
positive
Sheep (Ovis aries)
negative
negative
positive
Water buffalo (Bubalus arnee)
negative
negative
positive
Goat(Capra hircus)
negative
negative
positive
Fallow deer (Dama dama)
negative
negative
positive
Muscovy duck (Cairina sp.)
negative
negative
positive
Red deer(Cervus elaphus)
negative
negative
positive
Chicken (Gallus gallus)
negative
negative
positive
Turkey (Meleagris gallopavo)
negative
negative
positive
Atlantic Salmon (Salmo salar)
negative
negative
positive
sample
(reference sample from BfEL) see fig. 1
Real-time PCR Data
Horse detection
Standards/Regulations
Commission Directive 2002/86/EC
Commission regulation (EC) 999/2001
Commission regulation (EC) 1234/2003
German Food and Feed Law (LFGB)
German Food Labeling Regulation (LMKV)
Matrices
IAAC
(internal animal and
amplification control)
Horse sausage | meat balls | Chili con Carne | Kebab | Salami |
Gelatin | animal feed
Detection limit
The ANIMAL ID Horse & Donkey IAAC real-time PCR is developed
for the detection of horse- and donkey-DNA in meat mixture at a
relative amount of ≥ 0.1 %. The assay limit of detection depends
on sample matrix, processing grade, DNA-preparation and DNAcontent.
Donkey detection
Specificity
For verifying the specificity of ANIMAL ID Horse & Donkey IAAC
real-time PCR, DNA of reference material from BfEL (former
Bundesforschungsanstalt für Ernährung und Lebensmittel) and
potentially horse and donkey free food components were analysed.
For the horse and donkey real-time PCR detection method no cross
reactivity could be detected (see table 1).
Figure 1 ABI7500 real-time PCR assay with the SureFood ® ANIMAL ID
Horse & Donkey IAAC kit
Distribution and ordering
R-Biopharm AG
An der neuen Bergstr. 17
64297 Darmstadt, Germany
Phone: +49 (0) 61 51 - 81 02-0
Fax:
+49 (0) 61 51 - 81 02-20
E-Mail: [email protected]
www.r-biopharm.com
CONGEN Biotechnologie GmbH | Robert-Roessle-Straße 10 | 13125 Berlin
Tel: +49 30 9489-3500 | Fax +49 30 9489-3510 | e-mail: [email protected] | www.congen.de
Validation
Detection of Horse- and Donkey DNA with
SureFood® ANIMAL ID Horse & Donkey IAAC