BJP 5, BJP 10 & BJP 20
15X
30X
10X
20X
to achieve.
First,
locate
10X
20X the row with the
ad the Graduation Mark value at the top of
Operating Tips: Improving
Operating
speed of
Tips: Improving speed of
OPERATING
OPERATING INSTRUCTIONS
volume of the sample you are concentrating
Operating
Tips: Improving
Operating
speed of
Tips: Improving
speed of INSTRUCTIONS
concentration:
concentration:
Page
3
OPERATING
INSTRUCTIONS
OPERATING INSTRUCTIONS
Operating
Tips: Improving
Operating
speed of
Tips: Improving
speed
of
concentration:
concentration:
A. Determine
approximate
A. Determine
sample INSTRUCTIONS
approximate
D. sample
Once the desired volume
D. Once
is achieved,
the desired vol
ntil you
OPERATING
INSTRUCTIONS
OPERATING
Page
3
concentration:
concentration:
A.
Determine
approximate
Determine
samplecontent
approximate
D. sample
Once the desired
volume
D. concentrate
Once
is achieved,
the
desired
vol
microsolute
content
(forA.
microsolute
electrophoresis
or (for electrophoresis
oris withdrawn
the
concentrate
the
using
Pasteur,
is withdra
Speed of filtration is affected
Speed by
of several
filtration is affected
by
several
A.
Determine
Determine
sample
approximate
D.
Once
the
volume
D.
Once
is achieved,
the
desired
volt
microsolute
(forA.
microsolute
electrophoresis
content
or (forprotein
electrophoresis
oris loader
will be
immunoelectrophoresis
immunoelectrophoresis
protein
concentration
concentration
the
concentrate
withdrawn
the
concentrate
using
Pasteur,
is loader
withdra
Speed
of filtration
is affected
Speed
by
of several
filtration
by
several
Page
3including
thinsample
plastic
or desired
gel
thin
type
plastic
pipettes.
or
gel
The
parameters
temperature,
parameters
pH
including
and is affected
temperature,
pHcontent
andapproximate
BJP
BJP
BJP
BJP
CLINICAL
CLINICAL
CLINICAL
CONCENTRATORS
CONCENTRATOR
CLINICAL
CLINICAL
microsolute
content
microsolute
electrophoresis
content
or (forprotein
electrophoresis
oris loader
immunoelectrophoresis
immunoelectrophoresis
protein
concentration
concentration
the
concentrate
withdrawn
the
concentrate
using
Pasteur,
is loader
withdrat
Speed
of filtration
is affected
Speed
by
of several
filtration
by be
several
should
approximately
25 mg/mL)
be
approximately
25
thinmg/mL)
plastic
or gel
thin
type
plastic
pipettes.
or
gel
The
parameters
including
temperature,
parameters
pH
including
and is affected
temperature,
pH
and (forshould
sample
is
now
ready
for
sample
further
is
analysis.
now
ready
for f
protein
concentration.
While
protein
BJP
concentration.
While
BJP
concentrators will provide
concentrators
rapid filtration
will in
provideshould
rapid filtration
in
be approximately
should
be approximately
25
mg/mL)
sample
is now
furtherisanalysis.
now ready for f
protein
concentration.
protein
BJP
concentration.
BJP
B.rapid
Pipette
sample
B.25 mg/mL)
Pipette
aperture
sample
at thethrough
aperture
at ready
the forsample
concentrators
will provide
concentrators
rapid
filtration
will in
provide
filtration
in through
most
environments,
theWhile
most
following
environments,
theWhile
following
Operating Tips: Improving speed
of
B.
Pipette
sample
through
B.
aperture
sample
at the
through
concentrators
willbeprovide
concentrators
rapid
filtration
willbe
in
provide
rapid
filtration
inDevice
top
the
device.
top
canofPipette
be
theleft
device.
Device
can aperture
be left at the
most environments,
theOPERATING
most
following
the following
suggestions
may
helpful
suggestions
inenvironments,
optimizing
may
helpful
inofPipette
optimizing
INSTRUCTIONS
B.
sample
through
B.
Pipette
aperture
sample
atisthe
through
aperture
Operation:
Concentrating
macromolecules
Operating
Tips: Improving
speed
of
top
of
the
device.
Device
top
can
of
be
the
left
device.
Device
can
be left atisthe
concentration:
most
environments,
the
most
following
environments,
the
following
unattended
until
desired
unattended
concentration
until
desired
concentration
TECHNICAL
INFORMATION
TECHNICAL
INFORMATION
&
OPERATING
&
INSTRUCTIONS
OPERATING
INSTRUCTIONS
suggestions
mayspeed
be helpful
suggestions
in Filtration
optimizing
mayINSTRUCTIONS
be helpful
in optimizing
speed. Filtration
will
speed.
increase
speed
willtop
increase
OPERATING
of
the
device.
Device
top
can
of
be
the
left
device.
Device
can
be left is
Operating
Tips: Improving speed
of
unattended
until
desired
unattended
concentration
until
is
desired
concentration
concentration:
suggestions
may
be
helpful
suggestions
inDetermine
optimizing
may
be
helpful
in optimizing
achieved. D. Once the desired
A.
approximate
sample
volume is achieved,
speed.
Filtration
will
speed.
increase
Filtration
speed
willachieved.
increase
Operation:
Concentrating
macromolecules
proportionally
tospeed
ambient
proportionally
temperature.
to
ambient
temperature.
OPERATING
unattended
until desired
unattended
concentration
untilisOnce
desired
concentration
is
BJP 5, BJP
10 &
BJP 20
BJP
5, BJP
10
&
BJP 20INSTRUCTIONS
achieved.
achieved.
A.
Measure
sample
total
protein
to
D.
the
desired
volume
concentration:
speed.
Filtration
speed
will
speed.
increase
Filtration
speed
will
increase
proportionally
to
ambient
proportionally
temperature.
to
ambient
temperature.
A.
Determine
approximate
sample
D.
Once
the
desired
volumeisusing
isachieved,
achieved,
microsolute
content
BJPelectrophoresis
Applications orBJP Applications
Features
Features
Should
you require faster
Should
concentration,
you require
faster(for
concentration,
the
concentrate
is
withdrawn
Pasteur,
Speed of filtration is affected by
several
achieved.
achieved.
BJP concentrators
offer
BJP
fast
concentrators
and
convenient
offer
means
fast
to
and
convenient
means
to
proportionally
to
ambient
proportionally
temperature.
to
ambient
temperature.
determine
the
desired
concentration
factor.
C.
Solvent
and
micromolecules
C.–prior
Solvent
are
and
pulled
micromolecules
are
pulled
the
concentrate
is
withdrawn
using
Pasteur,
Should
you
require faster
Should
concentration,
you
require
faster
concentration,
A.
Determine
approximate
sample
D.
Once
the
desired
volume
is
achieved,
microsolute
content
(for
electrophoresis
or
place
the
concentrator
near
place
a
the
source
concentrator
of
heat.
near
a
source
of
heat.
immunoelectrophoresis
protein
concentration
the
concentrate
is
withdrawn
using
Pasteur,
Speed
of
filtration
is
affected
by
several
–
Concentration
of
urine
Concentration
to
electrophoresis
of
urine
prior
to
Integrated
electrophoresis
dead
stop
uses
Integrated
plastic
A.
Measure
sample
total
protein
to
D.
Once
the
desired
volume
is
achieved,
thin
plastic
or
gel
loader
type
pipettes.
The dead sto
parameters including temperature,
pH
and
prepare multiple
clinical
prepare
samples
multiple
forconcentration,
analysis
clinical
byof
samples
for
analysis
by
C.
Solvent
and
micromolecules
C.
Solvent
are
and
pulled
micromolecules
are
pulled
Should
you
require
Should
you
require
faster
concentration,
through
the
membrane
through
by afor
high
the
capacity
membrane
by
agel
highloader
capacity
(For
urine
samples
see
below
“Suggested
place
the
concentrator
near
place
aless
the
source
concentrator
heat.
near
aprotein
source
of
heat.
for
diagnosis
of
multiple
myeloma
diagnosis
and
of
multiple
myeloma
instead
and
of
glasspipettes.
pasteurPasteur,
pipettes.
instead
microsolute
content
(for
electrophoresis
or
thin
plastic
or
type
The
An acid
sample
withfaster
a pH
An
of
acid
sample
than
5with
will
a pH
of
less
than
5
will
immunoelectrophoresis
concentration
the
concentrate
is
withdrawn
using
Speed
of
filtration
is
affected
by
several
should
be
approximately
25
mg/mL)
determine
the
desired
concentration
factor.
thin
plastic
or
gel
loader
type
pipettes.
The of glass pas
parameters
including
temperature,
pH
and
the
concentrate
ishigh
withdrawn
usinganalysis.
Pasteur,
C. isa amyloidosis.
Solvent
and
micromolecules
C.
Solvent
are
and
pulled
micromolecules
are
pulled
or immunoassay.
electrophoresis
ThisConcentration
isathe
or
done
immunoassay.
without
This
done
without
sample
is now
for
further
protein concentration. electrophoresis
While BJP
through
the
membrane
through
by aamyloidosis.
high
the
capacity
by a ready
capacity
place
the
concentrator
near
place
source
concentrator
ofconcentrate
heat.
near
source
heat.
Black
print
Black print for grad
absorbent,
aof
dead
stop feature
absorbent,
prevents
a membrane
dead
the
stop
feature
prevents
thefor graduations.
Factors”)
An
acid
sample
with a pH
An
of
acid
less
sample
than
5
with
will
a
pH
of
less
than
5
will
take
longer
to
concentrate
take
than
longer
a
neutral
to
than
a
neutral
immunoelectrophoresis
protein
concentration
should
be approximately
25
mg/mL)
sample
is now
ready
for
further
analysis.
(For
urine
samples
see
below
“Suggested
thin
plastic
orgel
gel
loader
type
pipettes.
parameters
including
temperature,
pHsample
and
aWhile
centrifuge,
pressure
oravaccum
centrifuge,
source.
pressure
or
source.
Absorbent
through
the
through
by atohigh
the
capacity
by
ato
high
capacity
Variety
of
volumes
to 20The
Variety
ml.The of volumes t
thin
plastic
or
loader
pipettes.
sample
is
now
ready
fortype
further
analysis.
protein
concentration.
BJP
absorbent,
a membrane
dead
stop feature
absorbent,
prevents
a membrane
dead
the
stop
feature
prevents
the
BJP 5, BJP 10 &
BJP 20
An
acid
with
a pH
An
of
acid
lessAbsorbent
sample
5vaccum
with
will
a pH
of–than
less
than
5 will
sample
concentrating
sample
dryness.
from
concentrating
dryness.
concentrators
will provide
rapid
filtration
take
longer
to in
concentrate
take
than
longer
athan
neutral
to
concentrate
neutral
Concentrate
spinal
fluid
– prior
Concentrate
to electrophoresis
spinal fluid prior
to electrophoresis
sample.
to
ashould
sample.
physiological
pH
will
to
a physiological
pH
will
be
approximately
25afrom
mg/mL)
pulls
solvent
and Adjustment
microsolute
pulls solvent
through
and
theAdjustment
microsolute
ultrafilter
through
the
ultrafilter
Concentration
Factors”)
absorbent,
a dead
stop
feature
absorbent,
prevents
a dead
the
stop
feature
prevents
the analysis.
sample
is
now
ready
for
further
analysis.
protein
concentration.
While
BJP
sample
from
concentrating
sample
to
dryness.
from
concentrating
to
dryness.
B.
Pipette
sample
through
aperture
at
the
sample
is
now
ready
for
further
take
longer
to
concentrate
take
than
longer
a
neutral
to
concentrate
than
a
neutral
concentrators
will
provide
rapid
filtration
in
for
diagnosis
of meningitis
forand
diagnosis
multiple
of meningitis and
Available
units
Available
sample.
asample.
physiological
Adjustment
pH
will
to
a physiological
pHthe
will
most environments, theconcentrating
following
resultthe
inAdjustment
faster
filtration.
result
inpocket
faster
filtration.
Bmultipleas individual
BDor as as individu
sample.
concentrating
An to
dead
stop
sample.
at
the
An
dead
stop
pocket
at
B. the
Pipette
sample
through
aperture
at theto
C
C
sample
from
concentrating
dryness.
from concentrating to dryness.
BJP concentrators
offer
fast and
convenient
means
sclerosis.
8 test blocks.
8 test
sample
aperture
thesclerosis.
sample.
Adjustment
to of
aB.
sample.
physiological
Adjustment
pH
will
toDevice
athrough
physiological
concentrators
will
rapid
filtration
in
top
ofPipette
the
device.
bepH
leftwill at sample
incell
faster
filtration.
result
incell
faster
filtration.
B
BD blocks. C
most
environments,
thebottom
following
C
each
prevents
bottom
filtration
each
to
dryness.
prevents
filtration
tocan
dryness.
suggestions
may
beprovide
helpful
inofresult
optimizing
top
of
the
device.
Device
can
be
left
B.
Pipette
sample
through
aperture
at
the
to prepare multiple
clinical
samples
for
analysis
by
B.
Pipette
sample
through
aperture
at
the
result
in
faster
filtration.
result
in
faster
filtration.
D
B
device.
Device
can
C
C
– Concentrate
bacterial
– Concentrate
in urinebacterial antigens B
in urine
most
environments,
thewill
following
unattended
until
desired
concentration
isisantigens
particles willtop
Suspended
tendoftothe
foul
particles
the
will
tend
to
foulbetheleft
suggestions
mayspeed
be helpful
in Suspended
optimizing
speed.
Filtration
unattended
until
desired
concentration
(Legionella,
Pneumonia,
Streptococcus
(Legionella,
Pneumonia,
B)
Streptococcus
B)
electrophoresis or
immunoassay.
This
isImproving
doneincrease
without
aofparticles
top
of
the
device.
Device
left
of
device.
Device
can
be
left
Speed
Concentration
Improving
Speed
ofparticles
Concentration
Suspended
willtop
Suspended
tend
tothe
foul
the
will
tend
to
foul
the
If you
are
not
filling
your
unit
are
to the
notfull
filling
level,
your
these
unit
tables
toBenefits
thewill
fullhelp
level,
determine
these tables
the
will help determ
filter
element
and
slow
filter
filtration
element
speed.
and
filtration
speed.
unattended
untilslow
desired
concentration
isIf you
Benefits
suggestions
may
be
helpful
in
optimizing
achieved.
speed.
Filtration
speed
will
increase
prior
tonot
immunoassay.
prior
tonot
immunoassay.
proportionally
to
ambient
temperature.
achieved.
Suspended
particles
willunattended
Suspended
tend
to foul
particles
the
will
tend
to
foul
the
If
you
are
filling
you
unit
are
to
full
filling
level,
your
these
unittables
to
the
will
full
help
level,
these
tables
the
willthe
help
element
and
filter
filtration
element
speed.
and
filtration
speed.
centrifuge,
or &
vacuum
source.
Absorbent
pads
concentration
factor your
you
want
tothe
achieve.
factor
For
you
instance,
want
toNo
using
achieve.
the
BJP
Fordetermine
10,
instance,
if the
using
starting
BJPdeterm
10,
unattended
until
desired
concentration
isisIfconcentration
Prefiltration
with
aslow
syringe
Prefiltration
filter
will
with
clarify
aslow
syringe
filter
will
clarify
until
desired
concentration
BJP pressure
5, BJP
10
BJP 20
Speed
of filter
filtration
is affected
Speed
by
ofachieved.
several
filtration
parameters
is affected
by
several
parameters
risk
of
over
concentration.
No
risk
of
over
conci
speed.
Filtration
willconcentration,
increase
. full
. will
If
you
are
not
filling
If
you
unit
are
to
the
not
filling
level,
your
these
unit
tables
to
the
full
help
level,
determine
these
tables
the
will
help
proportionally
tospeed
ambient
temperature.
filter
element
and
filter
filtration
element
speed.
and
slow
filtration
speed.
BJP
Applications
Features
concentration
factor
you
concentration
to
achieve.
factor
For
you
instance,
want
to
using
achieve.
the
BJP
For
10,
instance,
if
the
using
starting
the
BJPdeterm
–filter
Concentration
of your
proteins
–want
Concentration
and
antigens
of
in
other
proteins
and
antigens
in
other
you require
faster
Prefiltration
with
aslow
syringe
Prefiltration
filter
will
with
clarify
a
syringe
will
clarify
volume
of
the
sample
you
volume
are
concentrating
of
the
sample
is
you
2.0
are
mL
concentrating
and
you
need
is
to
2.0
concentrate
mL
and
you
to
20X,
need
to10,
con
including
temperature
pH
including
and
protein
temperature
concentration.
pH
and
protein
concentration.
pull solvent and Should
microsolute
through
the
ultrafilter
the
sample
and
result
inachieved.
the
faster
sample
filtration
and
result
in
faster
filtration
No glass breakage duringNo
sample
glass breakage
di
achieved.
BJP concentrators
offer
fastambient
and
convenient
means
to
clinical
or
research
samples.
clinical
or research
concentration
factor
you
concentration
want
to
factor
For
you
instance,
want
to
using
achieve.
the
BJP
For
instance,
ifmL
the
using
starting
BJPto
proportionally
to
temperature.
Solvent
and
micromolecules
are
Prefiltration
with
a syringe
Prefiltration
will
with
clarify
aand
syringe
filter
will
clarify
volume
of the
you
volume
are
concentrating
of
the
sample
issamples.
you
2.0
are
mL concentrating
and
you
need
is
to
2.0
concentrate
and
you
tothe
20X,
need
con
Should
you
faster
concentration,
the
sample
and
result
inC.
the
faster
sample
filtration
and
result
inmicromolecules
faster
filtration
While
BJP
concentrators
While
will
provide
BJP
concentrators
rapid
filtration
will
in
provide
rapid
filtration
in pulled
removal.
Easy
to10,
read.
Flexible
removal.
Easy
to10,
reai
match
the
2mL
starting
match
volume
the
atachieve.
2mL
left
and
starting
look
volume
across
until
at
left
you
and
reach
look
the
across
concentration
until
you
reach
the
Solvent
are
pulled
speed
andofimproved
analytical
speed
and
results
improved
analytical
results
place
the
concentrator
near
a at
source
heat.
concentrating the
sample.
A require
dead stop
pocket
the
–C.filter
Concentration
of
urine
prior
tosample
electrophoresis
Integrated
dead
stop
uses
plastic
volume
of
the
you
volume
are you
concentrating
of
sample
isyou
2.0
are
mL
concentrating
and
you
need
is
tothe
2.0
concentrate
mL
and
you
to
20X,
need
tothe
con
prepare multiple
clinical
samples
forconcentration,
analysis
byofconcentration.
the
sample
and
result
inC.
the
faster
sample
filtration
andmembrane
result
in will
faster
filtration
Solvent
and
micromolecules
are
pulled
sample
volumes
with
sample
wit
most
environments,
some
most
factors
environments,
will
slow
filtration.
some
factors
slow
filtration.
match
2mL
starting
match
volume
the
at the
2mL
left
and
starting
look
volume
across
until
at left
you
and
reach
look
across
concentration
until
you
reach
Should
you
require
speed
and
speed
and
results
improved
analytical
results
through
the
byby
athe
capacity
factor
you
needsample
to
reach
factor
(20X)
and
need
look
to
atinstead
reach
the
top
(20X)
of
and
column.
look
at
the
Your
top
sample
ofhigher
that
will
column.
bevolumes
Your
sa
following
following
Initial
protein
concentration.
Initial
protein
place
the
concentrator
near
source
heat.
for
diagnosis
of
multiple
myeloma
and
of that
glass
pasteur
pipettes.
through
theand
membrane
ahigh
high
capacity
bottom of each cell
prevents
filtration
dryness.
An acid
sample
withfaster
ato
pH
ofaless
than
5improved
will analytical
C.
Solvent
micromolecules
are
pulled
concentration
factors.
concentration
facto
match
the
2mL
starting
match
volume
the
at
2mL
left
and
starting
look
volume
across
until
at
left
you
and
reach
look
the
across
concentration
until
you
reach
the
speed
and
improved
analytical
speed
and
results
improved
analytical
results
C.
Solvent
and
micromolecules
are
pulled
electrophoresis
or
immunoassay.
This
is
done
without
factor
you
need
to
reach
factor
(20X)
you
and
need
look
to
at
reach
the
top
(20X)
of
that
and
column.
look
at
the
Your
top
sample
of
that
will
column.
be
Your
sa
following
concentration.
following
Initial
protein
concentration.
Initial
protein
the
membrane
by
a
high
capacity
concentrated
to
20X
when
concentrated
it
reaches
to
the
20X
100X
when
graduation
it
reaches
mark
the
on
100X
the
graduation
unit.
mark
on
the
un
concentration
levels
willthrough
concentration
have
a
significant
levels
will
have
a
significant
place
the
concentrator
near
a
source
of
heat.
amyloidosis.
Black
print
for
graduations.
absorbent,
a
dead
stop
feature
prevents
the
A.
Filtration
speed
will
A.
increase
Filtration
proportionally
speed
will
increase
proportionally
An
acid
sample
with
a
pH
of
less
than
5
will
absorbent,
amembrane
dead stop
feature
prevents
the
takepressure
longer toorconcentrate
than
a neutral
factor
you
need
to
reach
factor
(20X)
you
andneed
look
to
at100X
reach
the
top
(20X)
ofreaches
that
and
column.
look
at
the
Your
top
sample
ofbethat
will
column.
be onunits
Your
sa
through
the
by
a high
high
capacity
following
concentration.
following
Initialon
protein
concentration.
Initial
protein
concentrated
to
20X
when
concentrated
it
reaches
to
the
20X
when
graduation
it
mark
the
on
100X
the
graduation
unit.
mark
the
un
Individual
units
may
disposed
Individual
may
concentration
levels
willthrough
concentration
have
a
significant
levels
will
have
a
significant
a centrifuge,
vaccum
source.
Absorbent
the
membrane
by
a
capacity
effect
on
concentration
effect
speed.
While
concentration
a
highly
speed.
While
a
highly
Variety
of
volumes
to
20
ml.
to
ambient
temperature.
to
Should
ambient
you
temperature.
require
faster
Should
you
require
faster
absorbent,
a dead
stop feature
prevents
the
An
acid
sample
withto
a pH
ofthan
less
5 will
sample
from
concentrating
totodryness.
sample
from
concentrating
dryness.
take
longer
to concentrate
athan
neutral
after
use.
Blocks
may have
after
tomark
be
use.on
Blocks
ma
concentrated
to
20X
when
concentrated
it
reaches
to
the
20X
100X
when
graduation
it
reaches
mark
the
on
100X
the
graduation
unit.
the
un
–
Concentrate
spinal
fluid
prior
to
electrophoresis
concentration
levels
will
concentration
have
a
significant
levels
will
have
a
significant
sample.
Adjustment
a
physiological
pH
will
absorbent,
a
dead
stop
feature
prevents
the
effect
on
concentration
effect
speed.
on
While
concentration
a
highly
speed.
While
a
highly
pulls solvent and microsolute through
the
ultrafilter
concentration,
place
thewill
concentration,
concentrator
place
a the
source
concentrator
near
a source
dilute
sample
concentrate
dilutenear
sample
rapidly,
once
concentrate
rapidly,
once
absorbent,
awill
dead
stop feature
prevents
the .
.
sample
from
concentrating
to
dryness.
with sample
stored with sample
take
longer
to concentrate
than
a neutral
BJP Applications
for
diagnosis
of meningitis
and
multiple
Available
asstored
individual
unitsDresidue.
effect
on concentration
effect
speed.
on
While
concentration
awill
highly
speed.
While
aonce
highly
sample.
a heat.
physiological
pHthe
will
dilute
sample
will
concentrate
dilute
sample
rapidly,
once
concentrate
rapidly,
of
of
heat.
result
inAdjustment
faster
filtration.
sample
from
toConcentration
dryness.
Dor as
concentration
macromolecular
exceeds
2concentrating
concentration
%
exceeds
2%
TABLE
1:
TABLE
1:
concentrating
the
sample.
An to
dead
stopmacromolecular
pocket
at
B C Factors
C
D Concentration
D C
B
B
C Factors
sample
from
concentrating
to
dryness.
sclerosis.
8
test
blocks.
dilute
sample
will
concentrate
dilute
sample
rapidly,
will
once
concentrate
rapidly,
once
sample.
Adjustment
to
a
physiological
pH
will
macromolecular
concentration
macromolecular
exceeds
2
concentration
%
exceeds
2
%
TABLE
1:
Concentration
TABLE
Factors
1:
Concentration
Factors
result
in
faster
filtration.
D
thedryness.
speed
of filtration
the
rapidly
speed
decrease.
rapidly
B
C
bottom of each
cellprior
prevents
filtration
B. An to
acidic
sample
with
B. a An
pHwill
acidic
of less
sample
thanof
5filtration
with
will a pHwill
of less
5 will
BJPthan
20decrease.
unit
BJP 20 unit
— Concentration
of urine
to electrophoresis
for
D
B
C
macromolecular
concentration
macromolecular
exceeds
% arewill
exceeds
2%
TABLE
1:decrease.
TABLE
Factors
1:1:Concentration
Factors
TABLE
1:Concentration
Concentration
TABLE
Factors
Concentration
Factors
the
speed
of filtration
rapidly
speed
of
decrease.
filtration
rapidly
result
in faster
filtration.
Static
concentrators
arewill
Static
not
practical
concentrators
for2concentration
take
longer
to
concentrate
takethan
longer
athe
neutral
to
concentrate
sample.
than
a not
neutral
sample.
–
Concentrate
bacterial
SBJP
tpractical
aantigens
rt20
Vounit
l. for in urine SBJP
tart20Vounit
l.
GraB
duation MarkC GraduatioD
n Mark
Suspended
particles
will
tend
to
foul
the
Concentration
Factors
diagnosis of multiple myeloma and amyloidosis.
the
speed
of filtration
the
rapidly
speed
of
decrease.
filtration
rapidly
Static
Static
not
practical
for
are
not
for 5X
unit
concentrations
aboveare
5will
concentrations
%.
above
5will
%.
tpractical
art20Streptococcus
Vdecrease.
oin
l. faster
SBJP
ta10X
rt20Vounit
l.
G
raduation10X
Mark
G
raduation 50X
M
ark
Adjustment
to
aconcentrators
physiological
pH
willto(Legionella,
result
aconcentrators
physiological
in faster
pH
willSBJP
result
5X
25X
50X
25X
100X
200X
100X
Pneumonia,
B)
Improving
Speed
ofparticles
Concentration
Suspended
will
tend to
foul
the Adjustment
If
you
are
not
filling
your
unit
to
the
full
level,
these
tables
will
help
determine
the
filter
element
and slow
filtration
speed.
Static
concentrators
are
Static
not
practical
concentrators
for
are
not
practical
for
Benefits
above 5 concentrations
%. prior to immunoassay.
above 5 %. S20tamL
rt Vol.
S20tamL
rt Vol.
G
raduation10X
Mark
G
raduation 100X
M
ark
5X
10X
5X
25X
50X
25X
100X
50X
200X
100X
filtration.concentrations
filtration.
10X
20X
10X
50X
20X
100X
50X
200X
400X
200X
If
you
are
not
filling
your
unit
to
the
full
level,
Table
1
will
help
determine
the
concentration
— Concentrate spinal
fluid
prior
to
electrophoresis
for
Suspended
particles
will
tend
to
foul
the
you
are
not
filling
your
unit
to
the
full
level,
these
tables
will
help
determine
the
filter
element
and
slow
filtration
speed.
concentrations
above
5 concentration
concentrations
%.
above
5 %.you
factor
want to achieve.
instance,
using
the
BJP 10,
if37.5X
the starting
5X
10X
5X
25X
10X
50X
25X
100X
50X
200X
100X
with a syringe
filter
will clarify
20
10X
20
mL
20X
10X
50X
20X
100X
50X
200X
100X
400X
200X
Speed of Prefiltration
filtration is affected
by several
parameters
15 mL
7.5X
15For
15X
7.5X
37.5X
15X
75X
150X
75X
300X
150X
Recovering
dry
samples
Recovering
dry
samples
No
risk
of
over
concentration.
C.
Suspended
particles
C.
will
Suspended
lend
to
foul
particles
the
filter
will
lend
to
foul
the
filter
factor
you
want
toofachieve.
First,
the
row
with the
sample
volume
for
yourthe
BJP75X
diagnosis of meningitis
and
multiple
sclerosis.
. full
If
are
not
filling
your
unit
to
the
level,
these
tables
willthe
help
element
and
filtration
speed.
20
10X
20
20X
10X
50X
20X
100X
50X
200X
100X
400X
200X
concentration
factor
you
want
tolocate
achieve.
instance,
using
BJPdetermine
10,
if37.5X
the
starting
–you
Concentration
proteins
and
antigens
inFor
other
15
7.5X
15
15X
7.5X
37.5X
15X
75X
150X
300X
150X
Prefiltration
with
aslow
syringe
filter
will
clarify
10
mL
5X
10
mL
10X
5X
25X
10X
50X
25X
100X
50X
200X
100X
Recovering
dry samples
Recovering
dry
samples
volume
of
the
sample
you
are
concentrating
is
2.0
mL
and
you
need
to
concentrate
to
20X,
includingfilter
temperature
pH
and
protein
concentration.
the
sample
and
result
in
faster
filtration
No
glass
breakage
during
sample
element and slow filtration
element
speed.
and
Prefiltration
slowThen,
filtration
with
speed.your
Prefiltration
with
model.
locate
desired
concentration
factor
in
that
row.
Finally,
read
thestarting
15
mL
7.5X
15
mL
15X
7.5X
37.5X
15X
75X
37.5X
150X
75X
300X
150X
10
5X
10
10X
5X
25X
10X
50X
25X
100X
50X
200X
100X
Recovering
dry
samples
Recovering
dry
samples
clinical
or
research
samples.
5
mL
2.5X
5
mL
5X
2.5X
12.5X
5X
25X
12.5X
50X
25X
100X
50X
concentration
factor
you
want
to
achieve.
For
instance,
using
the
BJP
10,
if
the
Prefiltration
with
a
syringe
filter
will
clarify
volume
of
the
sample
you
are
concentrating
is
2.0
mL
and
you
need
to
concentrate
to
20X,
BJP
concentrators
have
BJP
an
impermeable
concentrators
have
an
impermeable
the
sample
and
result
in
faster
filtration
While BJPbacterial
concentrators
willinprovide
rapid
filtration
removal.
Easy
tothe
read.
Flexible 50X
match
2mL
volume
at 5X
left and10
look
across5X
until
you10X
reach
concentration
a syringe
filter
will clarify
ain
syringe
the sample
filterand
willthe
result
clarify
inthestarting
sample10
and
result in
— Concentrate
urine
(Legionella,
speed
andantigens
improved
analytical
results
mL
mL
10X
25X
50X sample
25X
100X
200X
100X
52impermeable
52 mL
5X
2.5X
12.5X
5X
25X
12.5X
50X
100X
1Xthat column.
2X
1X
5X
2X
10X
5X
20X
10X
40X
20X
Graduation
Mark
value
atmL
the
of2.5X
This
isand
where
your
should
be25X
BJP
concentrators
have
BJP
anstop)
impermeable
concentrators
have
anstop)
volume
of pocket
the
sample
you
aretop
concentrating
is top
2.0
mL
you
need
tothe
concentrate
to
20X, 50X
concentrate
(dead
concentrate
which
(dead
which
the
sample
andB)result
in
faster
filtration
sample
with
higher
faster
filtration
speed pocket
and
faster
improved
filtration
analytical
speed
and
results
improved
analytical
results
most environments,
some
factors
will
slow
filtration.
match
the
2mL
starting
volume
at 2.5X
left
and
look
across
until
youvolumes
reach
concentration
speed
andconcentration.
improved
analytical
results
Pneumonia,
Streptococcus
prior
to
immunoassay.
factor
you
need
to
reach
(20X)
and
look
at
the
of
that
column.
Your
sample
will
be
5
mL
5
mL
5X
2.5X
12.5X
5X
25X
12.5X
50X
25X
100X
50X
following
Initial
protein
2
1X
2
2X
1X
5X
2X
10X
5X
20X
10X
40X
20X
BJP
concentrators
have
BJP
an
impermeable
concentrators
have
anstop)
impermeable
removed.
For
instance,
using
the
BJP
10,
if
the
starting
volume
of
the
sample
you
are
concentrate
pocket
(dead
concentrate
stop)
which
pocket
(dead
which
following
concentration.
following
concentration.
concentration
factors.
impedes
concentration
to
impedes
dryness.
concentration
However,
to
dryness.
However,
match
the 2mL
starting
volume
at 1X
left
and
look
across
untilmark
you2X
reach
concentration
speed
andconcentration.
improved
analytical
results
2BJP
mL
2BJP
mL
2X
5X
10XYour
5X
20X will 10X
40X
20X
factor
you
need
to
(20X)
and
look
at100X
the
top
that
column.
sample
be
10
unit
10graduation
unitof 1X
following
Initialconcentrate
protein
concentrated
to
20Xreach
when
ityou
reaches
the
on
thethe
concentration
levels
will
have
significant
pocketinadvertently
(dead
concentrate
stop)
which
pocket
(dead
stop)
which
A. Filtration
speed
will
increase
proportionally
impedes
concentration
to
impedes
dryness.
concentration
However,
to
dryness.
However,
concentrating
is
2.0
mL
and
need
to concentrate
toG20X,
match
the
2unit.
mL
starting
ifa the
concentrate
iflevels
the
concentrate
remains
too
inadvertently
remains
— Concentration
of proteins
and
antigens
in
other
clinical
unit
SBJP
tar(20X)
t10
VoHowever,
l. and
SBJP
tthe
art10Vgraduation
ounit
l. of that
radmark
u
ationon
M
aYour
rk may
Grbe
adudisposed
awill
tion be
Mark
D. have
Initial
protein
concentration
D. Initial
protein
will
concentration
have
ato
levels
will
have
atoolook
factor
you
need
to
reach
at100X
top
column.
sample
following
concentration.
Initial
protein
concentrated
20X
when
it
reaches
the
the
unit.
Individual
units
concentration
levels
will
a
significant
impedes
concentration
to
impedes
dryness.
concentration
However,
to
dryness.
if
theinconcentrate
inadvertently
if
theinremaining
concentrate
remains
inadvertently
remains
tooyou reach
effect
on concentration
speed.
While
a highly
to ambient
temperature.
Shouldsignificant
you
require
faster
long
the
concentrator,
long
the
concentrator,
the
volume
atconcentration
lefta too
and
look
the
factor
need
SBJP
tacross
aremaining
rt10
Vounit
l.until
SBJP
ta10X
rt10
Vounit
l.concentration
G
raduation10X
Mark youG
raduatto
ionreach
M
ark
or research
samples.
5X
25X
50X
25X
100X
200X
100X
effect
on concentration
significant
effect
speed.the
on
While
speed.
While
a 5X
after
use.
Blocks
may
have
ton 50X
be
concentrated
to
20X
when
it
reaches
graduation
mark
on
the unit.
if
the
inadvertently
if
theinremaining
concentrate
remains
too
inadvertently
too the 100X
concentration
levels
will speed.
havenear
arapidly,
significant
long
the
concentrator,
long
the
the
concentrator,
the
effect
on concentration
While
aonce
highly
S10rapidly.
taremaining
rof
t remains
Vand
olOnce
. the
S10tamL
rt sample
Vol.
G
radbe
uatconcentrated
ion10X
Mark
G
rad20X
uatiowhen
M
arit
k
solvent
will
eventually
evaporate
solvent
will
and
eventually
the
evaporate
concentration,
thewill
concentrator
ainconcentrate
source
10X
25X
10X
50X
25X
100X
50X
200X
100X
dilute place
sample
concentrate
mL
5X
10X
5X
25X
50X
25X
100X
50X
200X
100X
(20X)
and
look
at
the
top
that
column.
Your
will
to
highly dilute
sample
will
highly
concentrate
dilute
sample
rapidly.
will
Once
concentrate
. the concentrator, the remaining
stored
with
sample
residue.
long
in
the
concentrator,
long
the
in
remaining
solvent
will
eventually
evaporate
solvent
will
and
eventually
the
evaporate
5X
10X
5X
25X
10X
50X
25X
100X
50X
200X
100X
effect
on concentration
speed.
While
aonce
highly
mL and
mL
10X
25X
10X
50X
25X
100X
50X
200X
100X
510
mL
2.5Xthe unit.
510mL
5X
2.5X
12.5X
5X
25X
12.5X
50X
25X
100X
50X
sample
may
go
to dryness.
sample
Should
may
this
go
occur,
to dryness.
Should
this
occur,
protein
exceeds
protein
2%
concentration
the
speed
of
exceeds
2%
the
speed
ofthe
dilute sample
will
concentrate
rapidly,
of heat. macromolecular
reaches
the
100X
mark
concentration
exceeds
2eventually
%
TABLE
1:
Concentration
Factors
Features
Benefits
D on
• concentration
Use
plastic
instead
•Static
Use
plastic
of
glass
instead
pipettes
glass
pipettes
Bgraduation
Cof
solvent
will
evaporate
solvent
will
and
eventually
the
evaporate
the
mL
5X
10
mL
10X
5X
25X
10X
50X
25X
100X
50X
200X
100X
5Should
mL
2.5X
5 mL
5X
2.5X
12.5X
5X
25X
12.5X
50X
25X
100X
50X
may
go
toretrieved
dryness.
sample
may
this
go
occur,
toretrieved
dryness.
thisby
occur,
2.5
mLand
1.3X
2.5
2.5X
mL
1.3X
6.3X
2.5X
12.5X
6.3X
25X
12.5X
50X
25X
filtration sample
will
rapidly
decrease.
filtration
will
concentrators
rapidly
decrease.
Static
concentrators
proteins
may
be
proteins
toShould
solution
may
be
by
to10
solution
dilute
sample
will
concentrate
rapidly,
once
macromolecular
concentration
exceeds
2
%
TABLE
1:
Concentration
Factors
the speed
of filtration
will
rapidly
mLmL
2.5X
5X
2.5X
12.5X
5X
25X
12.5X
50X
25X
100X
50X
sample
go
toretrieved
dryness.
sample
Should
may
this
go
toretrieved
Should
thisbyoccur,
1.3X
2.5X
mL
1.3X
6.3X
2.5X
12.5X
6.3X
25X
12.5X
50X
25X
252.5
252.5mL
2X
-5X
2X
10X
5X
20X
10X
40X
20X
B. dead
An acidic
sample
with
a pHto
ofover
less
thandecrease.
5may
will
proteins
may
be
proteins
to
solution
may
be
byoccur,
to
solution
are
not
practical
for
concentrations
are
not
practical
above
for
5%.
concentrations
above
5%.
BJP
20
unit
pipetting
approximately
pipetting
100
µl
of1:
approximately
buffer
indryness.
100
µl
of
buffer
in-Integrated
stopspeed
Noconcentration
risk
macromolecular
exceeds
2 be
%retrieved
TABLE
Concentration
Factors
TABLE
1:
Concentration
Factors
the
of filtration
rapidly
decrease.
2.5
1.3X
2.5
2.5X
1.3X
6.3X
2.5X
12.5X
6.3X
25X
12.5X
50X
25X
2
mL
2
mL
2X
5X
2X
10X
5X
20X
10X
40X
20X
proteins
may
proteins
to
solution
may
be
by
retrieved
to
solution
by
•
Easy
to
read
•
concentration
Easy
to
read
factors
concentration
factors
1.5
mL
-1.5
1.5X
mL
-3.8X
1.5X
7.5X
3.8X
15X
7.5X
30X
15X
Static
concentrators
arewill
not
practical
for
pipetting
approximately
pipetting
100
µl
of
approximately
buffer
in
100
µl
of
buffer
in
BJP
20
unit
take longer
to concentrate
than
a
neutral
sample.
Stpocket
aout
rt of
Vothe
l. concentrate pocket several
Graduation Mark
and out of the concentrate
and
several
concentration.
2X
2X
10X
5X
20X
10X
40X
20X
1.5X
mL
3.8X
1.5X
7.5X
3.8X
15X
7.5X
30X
15X
the
speed of filtration
rapidly
decrease.
121.5
mLmL
121.5mL
---5X
2.5X
-5X
2.5X
10X
5X
20X
10X
Recovering
Samples
pipetting
approximately
pipetting
100
µl
of
buffer
in
µl
of buffer
Static
not
for
and Dry
out
of
the Recovering
concentrate
and
pocket
of
several
pocket
severalin-unit
concentrations
aboveare
5will
%.
SBJP
tDry
aout
rt20
Vapproximately
othe
l. concentrate
G
r
a
d
u
a
t
i
o
n
M
a
r
k
times.
times.
Adjustment
to aconcentrators
physiological
pH
will practical
result
in Samples
faster
5X 1001.5
10X
25X
50X
100X
200X
1.5
1.5X
mL
--3.8X
1.5X
3.8X
15X
7.5X
30X
15X
1pocket
mLmL several-1 mL
-2.5X
--7.5X
5X
2.5X
10X
5X
20X
10X
andduring
out stop
ofhave
the BJP
concentrate
and
of
several
concentrate
Static
are
not
for
times.
times.
concentrators
an
impermeable
concentrators
concentrate
impermeable
concentrate
Uses plastic
instead
of concentrators
No
glass
breakage
• practical
Dead
prevents
• Dead
stop
concentration
prevents
concentrations
above
5BJP
%.
S20tpocket
aout
rtover
Vhave
othe
l. an
G
d
ation 100X
M
ark
5X BJP
10X
25X
50X
100X
200X 5X20X
filtration.
mL
10X
20Xconcentration
50X
200X 2.5X
1 mLover
-1ra
mL
--5uunit
--2.5X
--5X
10X400X
10X
5 unit
BJP
times.
times.
pocket
(dead
stop)
which
pocket
impedes
(dead
concentration
stop)
which
to
impedes
concentration
to
concentrations
above
5removal.
%. Following are the concentration
Following
are thefor
concentration
glass Pasteur pipettes
sample
5X SBJP
10X
25X
50X
100X
200X
20
mLfactors
10X
20X
50X
100X
200X
400X
15inadvertently
7.5X
37.5X
150X
tart5factors
Vunit
o15X
l. for
SBJP
tart5 Vunit
ol. 75X
Graduation M
ark
Grad300X
uation Mark
Recovering
dry
samples
dryness.
However,
ifbiological
thethe
dryness.
concentrate
However,
ifbiological
thethe
concentrate
inadvertently
C. Suspended
particles
will
lend
to•foul
the filter
Following
are
concentration
Following
factors
are
the
for
concentration
common
solutions.
common
Locate
solutions.
SBJP
tmL
aLocate
rt5factors
Vunit
o15X
l.thefor5X
SBJP
ta10X
rt5 Vunit
ol. 75X
G
raduation10X
M
ark
G
rad300X
uation 50X
Mark
Volumes
up
to
•
Volumes
20
mL
up
to
20
20
10X
20X
50X
100X
200X
400X
5X
25X
50X
25X
100X
100X
15
7.5X
37.5X
150X
10
mL
5X
10X
25X
50X
100X
200X
Recovering
dry
samples
remains
too
long
in
the
remains
concentrator,
too
long
the
in
remaining
the
concentrator,
the
remaining
Following
are
the
concentration
Following
factors
are
the
for
concentration
factors
for
Black print
for
graduations
Easy
to
read.
common
biological
solutions.
common
Locate
biological
thevolume
solutions.
element and slow filtration speed. Prefiltration
with
aLocate
rt graduation
Vo15X
l.the 5X
S5tmL
a10X
rt Vol. 75X
G
raduation10X
M
ark
G
rad300X
uation 50X
Mark
starting sample
volume
starting
and
the
sample
graduation
andS5tthe
5X
25X
50X
25X
100X
100X
mL
10X
25X
10X
50X
25X
100X
50X
100X
15
7.5X
37.5X
150X
10
mL
5X
10X
25X
50X
100X
200X
Recovering
dry
samples
5
mL
2.5X
5X
12.5X
25X
50X
100X
solvent
will
eventually
evaporate
solvent
will
and
eventually
the
sample
evaporate
may
and
the
sample
may
common
biological
solutions.
common
Locate
biological
thevolume
solutions.
Locate
the 5X
have
an impermeable
starting
volume
starting
and
the
sample
graduation
and
graduation
10X
25X
10X
50X
25X
100X
50X
100X
54 the
mL
54 mL
10X
5X
25X
10X
50X
25X
100X
50X
100X
4X
8X
4X
20X
8X
40X
20X
80X
40X
80X
mark
forsample
the
liquid
level;
mark
then
for
read
the
the
liquid
level;
then
read
the
a syringe BJP
filterconcentrators
will clarify the
sample
and
result
inthis
10
mL
5X
10X
25X
50X
100X
200X
go
to
dryness.
Should
go
occur,
to
dryness.
proteins
Should
may
this
be
occur,
proteins
may
be
52then
mL
2.5X
5X
12.5X
25X
50X
100X
Variety of volumes
20 mL Flexible
sample
volumes
1Xin the
2X
5X
10X
20X
40X 40X
starting
volume
and
the
sample
graduation
volume
and
the
graduation
543box
mL
543 mL
10X
5X
25X
10X
50X
25X
100X
50X
100X
4X
8X
4X
20X
8X
40X
20X
80X
80X
mark
forsample
the liquid
level;
mark
for
read
the
the
liquid
level;
then
read
therow5X
BJPtoconcentrators
have
anstop)
impermeable
3X
6X
3X
15X
6X
30X
15X
60X
30X
60X
concentration
factor
in starting
concentration
the
box
at
the
factor
row
at
the
concentrate
(dead
faster filtration
speed pocket
and improved
analytical
results
retrieved
towhich
solution
retrieved
pipetting
to
approximately
solution
by
pipetting
approximately
52then
mL
2.5X
5X
25X
50X
1Xin the
2X
5X
10X
20X
40X 30X
withhave
higher
concentration
mL
8X
4X
20X
8X
40X
20X
80X
40X
80X
mark
for theby
liquid
level;
mark
for
read
the
the
liquid
level;
then
read
therow4X
342.5
3X
342.5mL
6X
3X
15X
6X
30X
15X
60X
60X
mL
2.5X 12.5X
5X
mL
2.5X
12.5X
5X
25X
12.5X
50X100X
25X
50X
concentration
factor
in
concentration
the
box
at
the
factor
row
box
at
the
BJP
concentrators
an
impermeable
and
column
intersection.
and
To
column
calculate
intersection.
To
calculate
concentrate
pocket
(dead
stop)
which
of bufferHowever,
in and out
100ofµlthe
of buffer
concentrate
in and pocket
out of the concentrate
pocket
followingimpedes
concentration.
concentration100
to µl
dryness.
32box
mLmL
3X
32 mL
6X
3X
15X
6X
30X
15X
60X
30X
60X
2BJP
1Xin the
5X
10X
20X
2.5
5X
mL
2.5X
12.5X
5X
25X
12.5X
50X
50X
concentration
factor
in concentration
the
box
at
factor
row
atby2X
the
2X
4X
2X
10X
4X
20X
10X
40X40X 25X
20X
40X
factors.
10
unit
and
column
intersection.
and
TomL
column
calculate
intersection.
To2.5
calculate
volume,
divide
the initial
volume,
volume
divide
bythe
the
the initial
volume
therow2.5X
concentrate
pocket
(dead
which
several
times.
several
times.
impedes
concentration
to stop)
dryness.
However,
2.5
mL
5X
mL
2.5X
12.5X
5X
25X
12.5X
50X
25X
50X
if
the
concentrate
inadvertently
remains
too
2
mL
2X
mL
4X
2X
10X
4X
20X
10X
40X
20X
40X
1.5
1.5X G22.5
1.5
3X
1.5X
7.5X
3X
15X
7.5X
30X
15X
30X
and
column
intersection.
and
To
column
calculate
intersection.
To
calculate
BJP
10
unit
volume,
divide
the initialconcentration
volume,
volume
divide
by
the
the initial volume by the 2.5X
S
t
a
r
t
V
o
l
.
r
a
d
u
a
t
i
o
n
M
a
r
k
D. Initial
protein
concentration
levels
will
have
a
concentration
factor.
factor.
impedes
concentration
tounits
dryness.
However,
21 mLmLby the 2X
21 mL
4X
2X
10X
4X
20X
10X
40X
20X
40X
1.5
1.5X
1.5
3X
mL
1.5X
7.5X
3X
15X
7.5X
30X
15X
30X
Available as individual
units
Individual
may
be
-2X
-5Xark
2X
10X
5X
20X
10X
20X
if
theinconcentrate
inadvertently
remains
too
volume,
divide
the initialvolume,
volume
divide
the initial
long
the
concentrator,
the
remaining
Concentration
Factors
Concentration
Factors
concentration
concentration
SBJP
tart10
Vbyounit
lthe
. factor.
G
rad
uation 50X
M
5X volume
10X 1.5X
25X
100X 7.5X
200X 10X
significant
effect
on concentration
speed.
While
a factor.
3X
mL
1.5X
7.5X
3X
15X
30X
15X
30X
11.5mLmL
-11.5mL
2X
-5X
2X
10X
5X
20X
20X
if
the
concentrate
inadvertently
remains
too
or as 8 test blocks
disposed
after
use.
Blocks
concentration
factor.
concentration
factor.
long
in
the
concentrator,
the
remaining
S10tlevel,
amL
rtyour
VTable
olunit
. 1 to
d
uatithe
oyou
n concentration
M
solvent
will will
eventually
andOnce
theunit
10X
25X
50X
100X
200X
10X
25X
50X
100X
ifevaporate
you are
filling
your
if you
to the
arefull
filling
will5X
the
help
fulldetermine
the1 concentration
will
determine
factor
want
factor
First,
youlocate
want
the
to achieve.
row10X
withFirst,
the locate
highly dilute
sample
concentrate
rapidly.
1level,
mL Table
-- help G
1ra
mL
2X
-5Xarkto achieve.
2X
10X
5X
20X200X
20X the ro
may
have
to
bevolume
stored
with
long
in may
the
the
remaining
solvent
will concentrator,
eventually
evaporate
and
sample
for
your
sample
BP model.
volume
for
locate
youryour
BP model.
desiredThen,
concentration,
locate
desired
in12.5X
concentration,
that row. Finally,
factor
readinthe
that
Graduation
row. Finally,
Mark
read
value
the at
Graduation
the top ofMark value
5X
10Xyourfactor
25X
50X
100X
200X
10
mL
10X
25X
50X
100X
200X
5Then,
mL
2.5X
5X
25X
50X
100X
sample
go
to dryness.
Should
this
occur,
protein concentration
exceeds
2%
the
speed
ofthe
sample
residue.
that
column. and
This
where
thatyour
column.
sample
This
should
is where
be removed.
your
sample
For instance,
should
removed.
the 12.5X
BJP
For 10,
instance,
if the starting
using
BJP 10,of
ifthe
thesample
startingyou
volume
are concentrating
of the sample
you
solvent
will
eventually
evaporate
the
10mL
mL
5X
10Xbeusing
25X
50X thevolume
100X
200X
Page
3 ar
52.5
2.5X
5X
25X
50X
100X
may
go
dryness.
thisisby
occur,
mL
1.3X
2.5X
6.3X
12.5X
25X
50X
filtration sample
will rapidly
Static
concentrators
proteins
maydecrease.
betoretrieved
toShould
solution
is 2.0 mL and you need to
is 2.0
concentrate
mL and you
to 20%,
need match
to concentrate
the 2 mLtostarting
20%, match
volume
theat2left
mLand
starting
look volume
across until
at left
youand look across until you
Page 3
5
2.5X
5X
12.5X
25X
50X
100X
sample
may
go
to
dryness.
Should
this
occur,
2.5
mL
1.3X
2.5X
6.3X
12.5X
25X
50X
2
mL
-2X
5X
10X
20X
40X
proteins
may
be
retrieved
to
solution
by
are not practical
concentrations
above
pipettingforapproximately
100
µlconcentration
of5%.
buffer inreach
reach
the
factorthe
youconcentration
need to reachfactor
(20X)and
you need
look at
tothe
reach
top(20X)and
of that column.
look at the
Yourtop
sample
of that
willcolumn.
be
Your sample will be
Page 3
2.5mL
1.3X
2.5X
6.3Xon the unit.
12.5X
25X
50X
21.5
2X
5X
10X
20XADVANCED P
40X
proteins
may
retrieved
topocket
solution
bywhen
1.5X
3.8X
7.5X
15X
30X
concentrated
20X
it reaches
the
tomL
20X
100X
when
graduation
it -reaches
mark
theon
100X
the
graduation
unit.
mark
ROTEIN SEPARATIONS
ADVANCED PROT
pipetting
approximately
100
µl
of to
buffer
inconcentrated
and out of
thebeconcentrate
several
sample is now ready forsample
furtherisanalysis.
now ready for f
protein concentration. While
protein
BJPconcentration. While BJP
ADVANCED PROTEIN SEPARATIONS
immunoelectrophoresis
immunoelectrophoresis
protein
concentration
concentration
should
bepH
approximately
should
25 mg/mL)
be
approximatelyprotein
25
thinmg/mL)
plastic
or gel loaderthin
typeplastic
pipettes.
or gel
Theloader t
parameters including temperature,
parameters pH
including
and temperature,
and
BJP
BJP
TECHNICAL INFORMATION
& OPERATING INSTRUCTIONS
CONCENTRATORS
CONCENTRATORS
CLINICAL
CONCENTRATORS
TECHNICAL INFORMATION & OPERATING INSTRUCTIONS
CONCENTRATORS
Technical Information
Technical &Information
Operating Instructions
& Operating Ins
BJP 5, BJP 10 &BJP
BJP5, 20
BJP 10
• Use plastic instead of glass pipettes
• Easy to read concentration factors
Recovering
Drypipetting
Samples
-2X
5X
10X
20X
40X
1.5
1.5X
3.8X
7.5X
15X
30X
12 mLmL
-2.5X
5X
10X
20X
Recovering
100pocket
µl of buffer
and Dry
out Samples
ofapproximately
the concentrate
severalin
times.
1.5
mL
1.5X
3.8X
7.5X
15X
30X
1
mL
--2.5X
5X
10X
20X
and
outan
ofhave
the an
concentrate
pocket
several
times.
BJP concentrators
have
impermeable
concentrate
BJP concentrators
impermeable
concentrate
• Dead
stop
prevents
over
concentration
1 mL5 unit --2.5X
5X
10X
20X
BJP
times.
(dead
stop)
which
impedes
concentration
pocket pocket
(dead stop)
which
impedes
concentration
to
Following are the concentration factors to
for
SBJP
tart5 Vunit
ol.
Graduation Mark
However,
ifbiological
thethe
concentrate
dryness.dryness.
However,
if the concentrate
inadvertently
remains
Following
are
concentration
factors
common
solutions.
Locate
thefor
SBJP
tart5 Vunit
ol.
G
raduation 50X
Mark
• Volumes
up
to
20 inadvertently
mL
5X
10X
25X
100X
too
long inbiological
thethe
concentrator,
the
remaining
too longremains
in the concentrator,
remaining
solvent
will
Following
are
the
concentration
factors
common
solutions.
Locate
thefor
S5tmL
art Vol.
G
raduation 50X
Mark
starting sample volume and the graduation
5X
10X
25X
100X
10X
25X
50X
100X
solvent
will
eventually
evaporate
and
the
sample
may
eventually evaporate
and biological
the sample
may
gothe
to
dryness.
common
solutions.
Locate
the
starting
volume
and
graduation
10X
25X
50X
100X
5X
10X
25X
50X
100X
45 mL
4X
8X
20X
40X
80X
mark forsample
the liquid
level;
then
read
the
dryness.
Should
thisbe
occur,
proteins
may
beby
Should go
thistooccur,
proteins
may
retrieved
to
solution
starting
sample
volume
and
the
graduation
5X
10X
25X
50X
100X
4X
8X
20X
40X
80X
mark
for
the
liquid
level;
then
read
the
345 mL
3X
6X
15X
30X
60X
concentration
factor
in
the
box
at
the
row
retrieved
to solution
by
pipetting
approximately
pipetting
approximately
100
µlliquid
of buffer
inthen
andread
out of
the
4
4X
8X
20X
40X
80X
mark
for
the
level;
the
3
mL
3X
6X
15X
30X
60X
2.5
mL
2.5X
5X
12.5X
25X
50X
concentration
factor
in
the
box
at
the
row
column
intersection.
To calculatepocket
100 µlpocket
of and
buffer
in and
out of the concentrate
concentrate
several
times.
3 mLmL
3X
6X
15X
30X
60X
2.5X
5X
12.5X
25X
50X
concentration
factor
in the
atbythe
22.5
2X
4X
10X
20X
40X
and
column
intersection.
To box
calculate
volume,
divide
the initial
volume
therow
several times.
2.5X
5X
12.5X
25X
50X
22.5mLmL
2X
4X
10X
20X
40X
1.5
1.5X
3X
7.5X
15X
30X
and
column
intersection.
To
calculate
volume,
divide
the
initial
volume
by
the
concentration factor.
2 mLmL
2X
4X
10X
20X
40X
1.5X
3X
7.5X
15X
30X
11.5
-2X
5X
10X
20X
volume,
divide factor.
the initial volume by the
Concentration
Factors
concentration
1.5mLmL
1.5X
3X
7.5X
15X
30X
1
-2X
5X
10X
20X
concentration factor.
if you are filling your unit to the full level, Table 1 will help determine
the concentration
factor you want
1 mL
-2X
5X to achieve.
10X First, locate
20X the row with the
sample volume for your BP model. Then, locate your desired concentration, factor in that row. Finally, read the Graduation Mark value at the top of
that column. This is where your sample should be removed. For instance, using the BJP 10, if the starting volume of the sample you are concentrating
Technical Information & Operating Instructions
BJP 5, BJP 10 & BJP 20
Improving Speed of Concentration
Speed of filtration is affected by several parameters including temperature, pH and protein concentration. While BJP concentrators will provide rapid filtration
in most environments, some factors will slow filtration.
— Filtration speed will increase proportionally to ambient temperature. Should you require faster concentration, place the concentrator near a source of heat.
— An acidic sample with a pH of less than 5 will take longer to concentrate than a neutral sample. Adjustment to a physiological pH will result in faster
filtration.
— Suspended particles will tend to foul the filter element and slow filtration speed. Prefiltration with a syringe filter or centrifugation will clarify the sample
and result in faster filtration speed and improved analytical results following concentration.
— Initial protein concentration levels will have a significant effect on concentration speed. A highly dilute sample will concentrate rapidly. Once protein
concentration exceeds 2 G/dL the speed of filtration will rapidly decrease. Static concentrators are not practical for concentrations above 5 G/dL.
Suggested Concentration Factors for Urine Samples
For a BJP Concentration Factor Calculator please visit: www.vivaproducts.com/calculator.html
Suggested CAP Validation Procedures
For Recommended Procedures please visit: www.vivaproducts.com/downloads/lab-procedure-performing-the-test.pdf
To download the CAP Calculation Table please visit: www.vivaproducts.com/downloads/cap-recovery-table.xls
TECHNICAL SPECIFICATIONS
Concentration Capacity
Normal Volume
With optional expansion reservoir (BJPA-ER20)
Dimensions
For BJP-5/30 and 5/100, BJP-10/30 and 10/100, BJP-20/30 and 20/100
Width
Height
Depth
For BJP-5/40, 10/40
Width
Height
Depth
Active membrane area
Dead stop volume
Materials of Construction
Membrane
Reservoir
Typical Performance
Time to concentrate 10x min. 200 C
7,500 MWCO PES
BJP-5
BJP-10
BJP-20
Start volume
5 ml
10 ml
10 ml
Albumin (66,000 MW) (0.25 mg/ml)
30
60
55
lgG (160,000 MW) (0.25 mg/ml)
35
70
65
Time to concentrate 50x min. 200 C
Albumin (66,000 MW) (0.25 mg/ml)
40
80
75
lgG (160,000 MW) (0.25 mg/ml)
45
90
85
** All 20 ml samples start at 0.10 mg/ml
ORDERING INFORMATION
Type of Device
Individual device (no pipettes)
Individual device (with pipettes)
Individual device (no pipettes)
Individual device (with pipettes)
8 test block (no pipettes)
8 test block (with pipettes)
No. Tests
30
30
100
100
40
40
ACCESSORIES
Plastic disposable pipettes (qty of 250)
Plastic disposable pipettes (qty of 100)
Plastic disposable pipettes (qty of 40)
Plastic disposable pipettes (qty of 30)
Expansion reservoirs for use with BJP-20 devices (qty of 10)
Acrylic stand for BJP individual units
BJP-5
5 ml
NA
BJP-10
10 ml
NA
BJP-20
10 ml
20 ml
38 mm
100 mm
24 mm
38 mm
100 mm
24 mm
45 mm
100 mm
27 mm
147 mm
94 mm
70 mm
25 cm2
50 µl
147 mm
94 mm
70 mm
25 cm2
50 µl
NA
NA
NA
28 cm2
50 µl
Polyethersulfone (7,500 MWCO)
Acrylonitrile Butadiene Styrene Polymer
Concentrate recovery %
BJP-20
BJP-5
BJP-10
20 ml
5 ml
10 ml
110**
92%
92%
130**
65%
68%
Concentrate recovery %
150**
90%
90%
170**
56%
60%
BJP-5
BJP-5/30
BJP-5/30P
BJP-5/100
BJP-5/100P
BJP-5/40
BJP-5/40P
BJP-10
BJP-10/30
BJP-10/30P
BJP-10/100
BJP-10/100P
BJP-10/40
BJP-10/40P
BJP-20
10 ml
92%
68%
90%
60%
BJP-20
BJP-20/30
BJP-20/30P
BJP-20/100
BJP-20/100P
BJPA-P250
BJPA-P100
BJPA-P40
BJPA-P30
BJPA-ER20
BJPA-AS
ProChem • 521 Great Road • Littleton, MA 01460 • Toll-free phone (U.S. & Canada) 866-635-3738 • Fax 978-952-6143 • Ouside U.S. & Canada: 978-952-6185