BD Pharmingen™
Technical Data Sheet
PE Mouse anti-Human PI-16
Product Information
Material Number:
Alternate Name:
Size:
Vol. per Test:
Clone:
Immunogen:
Isotype:
Reactivity:
Storage Buffer:
563520
PI16; Peptidase inhibitor 16; CRISP-9; PSPBP; PSP94-binding protein; MSMBBP
50 tests
5 µl
CRCBT-02-001
Human PI16 Transfected Cell Line
Mouse IgG1, κ
QC Testing: Human
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Description
The CRCBT-02-001 monoclonal antibody specifically binds to human Peptidase inhibitor 16 (PI-16), also known as Protease inhibitor 16.
PI-16 is a glycophosphatidylinositol-linked, 436 amino acid surface glycoprotein. It is a member of the cysteine-rich secretory protein
(CRISP) family and is likewise known as CRISP9. PI-16 is a putative serine protease inhibitor and can serve as a binding protein for PSP94
(prostate secretory protein of 94 amino acids) termed PSPBP. Serum levels of PSP94 and PI-16 can reportedly serve as key targets for prostate
cancer research. PI-16 is highly expressed by the activated/memory, FoxP3-bright subsets of regulatory T cells. PI-16-positive regulatory T
cells expressed uniformly high levels of the chemokine receptors, CCR4 and CCR6, when compared with PI-16-negative T regulatory cells.
The results suggest that the CCR4+CCR6+ regulatory T cells are capable of homing to inflammatory sites and regulating effector T cells such
as CCR4+CCR6+ Th17-like cells. PI-16 is also expressed by subsets of memory CD4+ and CD8-bright T cells. It is not expressed by B cells
or monocytes.
Multicolor flow cytometric analysis of PI-16 expression on human peripheral blood CD4+CD25+CD127low T cells. Whole human peripheral blood
was stained with the BD Pharmingen™ Human Regulatory T Cell Cocktail (Cat. No. 560249) containing FITC Mouse Anti-Human CD4, PE-Cy™7 Mouse
Anti-Human CD25, and Alexa Fluor® 647 Mouse Anti-Human CD127 antibodies, and either PE Mouse IgG1, κ Isotype Control (Cat. No. 349043; Middle
Panel) or PE Mouse Anti-Human PI-16 antibody (Cat. No. 563520; Right Panel). Erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No.
555899). Two-color flow cytometric contour plots show the correlated expression patterns of PI-16 (or Ig Isotype control staining) versus CD25 for
CD4+CD25+CD127low-gated events (Left Panel) with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was
performed using a BD™ LSR II Flow Cytometer System.
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Application Notes
Application
Flow cytometry
563520 Rev. 3
Routinely Tested
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Suggested Companion Products
Catalog Number
554656
555899
560249
349043
Name
Stain Buffer (FBS)
Lysing Buffer
Human Regulatory T Cell Cocktail
PE Mouse IgG1 Isotype Control
Size
500 ml
100 ml
50 tests
100 µg
Clone
(none)
(none)
(none)
X40
Product Notices
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This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental
sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
Cy is a trademark of Amersham Biosciences Limited.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at
www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
References
Grose RH, Millard DJ, Mavrangelos C, Barry SC, Zola H, Nicholson IC, Cham WT, Boros CA, Krumbiegel D. Comparison of blood and synovial fluid th17 and
novel peptidase inhibitor 16 Treg cell subsets in juvenile idiopathic arthritis. J Rheumatol. 2012; 39(10):2021-2031. (Clone-specific: Flow cytometry)
Nicholson IC, Mavrangelos C, Bird DR, Bresatz-Atkins S, Eastaff-Leung NG, Grose RH, Gundsambuu B, Hill D, Millard DJ, Sadlon TJ, To S, Zola H, Barry S. PI16
is expressed by a subset of human memory Treg with enhanced migration to CCL17 and CCL20. Cell Immunol. 2012; 275(1-2):12-18. (Immunogen: Flow
cytometry)
Reeves JR, Dulude H, Panchal C, Daigneault L, Ramnani DM. Prognostic value of prostate secretory protein of 94 amino acids and its binding protein after radical
prostatectomy. Clin Cancer Res. 2006; 12(20 Pt 1):6018-6022. (Clone-specific)
Sadlon TJ, Wilkinson BG, Pederson S, Brown CY, Bresatz S, Gargett T, Melville EL, Peng K, D'Andrea RJ, Glonek GG, Goodall GJ, Zola H, Shannon MF, Barr.
Genome-wide identification of human FOXP3 target genes in natural regulatory T cells. J Immunol. 2010; 185(2):1071-1081. (Biology)
Whitaker HC, Warren AY, Eeles R, Kote-Jarai Z, Neal DE. The potential value of microseminoprotein-beta as a prostate cancer biomarker and therapeutic target.
Prostate. 2012; 70(3):333-340. (Biology)
563520 Rev. 3
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