Peak Scientific Gas Generators OEM Application List 2014 Copyright Peak Scientific - Rev. 5 Peak Scientific Applications List Contents AB Sciex 3 Agilent5 Biotage7 Bruker8 Dionex9 Jasco10 Jeol 11 Leco 12 Perkin Elmer 13 Shimadzu14 Thermo Scientific 16 Varian18 Waters19 All other applications 21 Sample Evaporation/ Concentration/ SPE 25 Multiple MC/MS selection guide 27 Gas Chromatography general application guide 28 Page 2 peakscientific.com Peak Scientific Applications List Application AB SCIEX API Q-STAR Requirements Curtain + Vent gas 4 L/min; 60 psi max Source 12L/min, 105 psi max Exhaust 8L/min 50 psi CQ-Star w/o MALDI: CAD gas; 1 L/min 60 psi Solution Infinity 1031 Genius ABN2ZA Genius AB- 3G AB SCIEX API Q-TRAP Curtain 0.5 – 3 L/min; 50 psi max Source 12L/min, 90 to 100 psig Exhaust 8L/min 90 to 100 psig Infinity 1031 Genius ABN2ZA Genius AB- 3G AB SCIEX API 100/ 150/ 165 Curtain 2 L/min; Source 13L/min, Exhaust 4L/min Infinity 1031 Genius ABN2ZA Genius AB- 3G AB SCIEX API 300/ 365 Curtain 2 L/min; Source 15L/min Exhaust 4L/min Infinity 1031 Genius ABN2ZA Genius AB- 3G AB SCIEX API 2000 LC/MS/MS Curtain 0.5 - 3 L/min; 50 psig Source 12L/min, 90 to 100 psig Exhaust 8L/min 50 psig Infinity 1031 Genius ABN2ZA Genius AB- 3G AB SCIEX API 3000 LC/MS/MS Curtain 3.5 L/min; 60 psi Source 12 L/min, 90 psi Exhaust 8 L/min 50 psi Infinity 1031 Genius ABN2ZA Genius AB- 3G AB SCIEX API 3200 LC/MS/MS 3200 QTRAP Curtain 5 L/min; 60 psi (max.) Source 22 L/min, 100–105 psi Exhaust 6.4 L/min 55 (min.)–60 psi (max.) Infinity 1031 Genius ABN2ZA Genius AB- 3G AB SCIEX API 4000 LC/MS/MS 4000 QTRAP Curtain 5 L/min; 60 psi max Source 17L/min, 90–105 psi Exhaust 6L/min 55 max Infinity 1031 Genius ABN2ZA Genius AB- 3G AB SCIEX API 4500 LC/MS/MS 4500 QTRAP Curtain 10 L/min; 60 psi max Source 22L/min, 100 psi max Exhaust 8L/min 55 max Infinity 1031 Genius ABN2ZA Genius AB- 3G AB SCIEX API 5000 LC/MS/MS Curtain 10 L/min; 60 psi Source 22.5 L/min, 55–60 psi (max.) Exhaust 6 L/min 55–60 psi (max.) Infinity 1031 Genius ABN2ZA Genius AB- 3G Curtain 10 L/min; 60 psi max Source 22 L/min, 100 psi max Exhaust 8 L/min 55 psi minimum Infinity 1031 Genius ABN2ZA Genius AB-3G Curtain 13 L/min; 60 psi max Source 22 L/min, 100 psi max Exhaust 17 L/min 55 psi minimum Infinity 1031 Genius 3031 AB SCIEX API 5500 LC/MS/MS API 5500 QTRAP *Without SELEXION AB SCIEX API 5500 LC/MS/MS API 5500 QTRAP *With SELEXION AB SCIEX Triple TOF 5600 Triple TOF 5600 + Curtain 10 L/min; 60 psi Source 22.5 L/min, 105 psi Exhaust 8 L/min 55 psi to 105 psi Infinity 1031 Genius ABN2ZA Genius AB-3G CAD gas uhp N2@ 1ml/min, 60psi TBA (No info from AB on this yet) Infinity 1031 Genius ABN2ZA, Genius AB-3G? Genius 3031? Curtain 18 L/min; 80 psi max Source 26 L/min, 100 psi max Exhaust 25 L/min 70 psi minimum Infinity 1030 Genius 3031 AB SCIEX Triple TOF 5600 + *With SELEXION AB SCIEX API 6500 LC/MS/MS API 6500 QTRAP *With or without SELEXION Page 3 peakscientific.com Peak Scientific Applications List AB Sciex continued Application Requirements Solution API w/ Photospray Application Same flow rates and pressures as above equivalent models. Infinity 1031 Genius ABN2ZA Genius AB-3G Genius 3031? Note: For previous AB Sciex models up to and including API5000, AB would state in their manuals that Zero-Air OR Nitrogen can be used for SOURCE GAS. This meant that you could operate an API LC/MS/MS with a single nitrogen-only gas source. For the newest AB Sciex models API5500, API4500 and the Triple TOF 5600, AB SCIEX recommend only Zero Grade Air to be used for SOURCE GAS (Gas 1/Gas 2) when operating a Nanospray Ion Source. When using UHP N2 with this ion source, there is an increased risk of corona discharge, which can damage the emitter tip. However; if customer is using the DuoSpray™ or Turbo V™ or PhotoSpray ion source, they can still use UHP N2 for Gas 1 and Gas 2. Page 4 peakscientific.com Peak Scientific Applications List Application Requirements Solution Agilent 1100 Series LC/MSD Nitrogen up to 15L/min, 80 - 100 psi ( At least 3L/min is required at all times to prevent air entering the instrument) Infinity NM18L Infinity NM32L Genius 1050 Genius NM32LA, Genius NM3G Agilent 1200 Series HPLC with ELS Detector Nitrogen up to 4L/min, 50 psi ( for G4218A ELSD) Infinity NM18L Genius N118LA NG4000A Agilent 385 ELSD Nitrogen 0.9 - 3.25 L/min @ 60 - 100 psi Infinity NM18L Genius N118LA NG3000A Agilent 6100 Series Nitrogen up to 15L/min, 80 – 100 psi ( At least 3L/min is required at all times to prevent air entering the instrument) Infinity NM18L Infinity NM32L Genius 1050 Genius NM32LA, Genius NM3G Agilent 6200 Series Nitrogen up to 18L/min, 80 – 100 psi Infinity NM32L Genius NM32LA Genius 1050 Genius NM3G Agilent 6300 Series Ion Trap LCMS Nitrogen up to 18L/min, 80 – 100 psi Infinity NM32L Genius NM32LA Genius 1050 Genius NM3G Agilent 6400 Series 6410 / 6420/ 6430 Nitrogen up to 18L/min, 80 – 100 psi ( At least 3L/min is required at all times to prevent air entering the instrument) Infinity NM32L Genius NM32LA Genius 1050 Genius NM3G Agilent 6460 Nitrogen up to 30L/min, 80 – 100 psi ( At least 3L/min is required at all times to prevent air entering the instrument) Infinity NM32L Genius NM32LA Genius 1050 Genius NM3G Agilent 6490 LCMS QQQ iFunnel Nitrogen up to 50L/min, 80 – 100 psi ( At least 9L/min is required at all times to prevent air entering the instrument) Genius 3010 Infinity 5010 Agilent CHIP CUBE Up to 30 L/min of nitrogen standard + Requires 4 L/min of air to be mixed in with the nitrogen supply to achieve 10% oxygen content for Chip Cube operation -------------------------------------------------------Up to 60 L/min of nitrogen standard. + Requires 10 L/min of air to be mixed in with the nitrogen supply to achieve 10% oxygen content for Chip Cube operation Chip Cube is available on all 6000 series LC/MS models. ------------------------------------CHIP CUBE on iFunnel models 6490 and 6550 Generator models with Switch for Chip-Cube operation: Genius 1023 Genius 3023 (2 x) ---------------------------------Genius 3013 Agilent 6500 Series QTOF LC/MS Nitrogen up to 20 L/min for 6510, 6520, 6538 Nitrogen up to 30 L/min for 6530 and 6540 ( At least 3L/min is required at all times to prevent air entering the instrument) Infinity NM32L Genius NM32LA Genius 1050 Genius NM3G 6550 iFunnel Q-TOF LC/MS Nitrogen up to 60 L/min for 6550 ( At least 12L/min is required at all times to prevent air entering the instrument) Infinity 5010 Genius 3010 Agilent models available with Jet Stream technology: Nitrogen 30 L/min @ 100 psi Infinity NM32L Genius NM32LA Genius 1050 Genius NM3G Page 5 peakscientific.com Peak Scientific Applications List Agilent continued Application Requirements Nitrogen for Plasma Operation: 22.5 L/min at 72.5 psi ( 99.5%+ ) Agilent 4100 MP-AES Microwave Plasma-Atomic Emission Spectrometer Nitrogen for Optics purge: 10 L/min at 65 psi (99.95%+) Clean, Dry, Oil-free Compressed Air for: Preoptics protection: 25 L/min at 72.5 psi Optional ECGM: 1.5 L/min at 72.5 psi Agilent 70 series ICP-OES High Purity Nitrogen 0.45 L/min in standby mode Up to 2.1 L/min in operational mode Solution To be advised. (Agilent offer own-brand 4107 PSA Nitrogen generator at the moment) NG Range Argon Gas Supply Minimum Purity: 99.99% Maximum Flow Rate: 20 L/min Supply Pressure: 700 kPa Agilent 7500 ICP-MS Reaction Gas Supply (Agilent 7500cs, 7500cx) Gas: Helium, Hydrogen (optional for 7500cx) Minimum Purity: 99.999% MaxFlow Rate: He 10 mL/min, H2 7 mL/min Typical Pressure: 40 kPa Precision Hydrogen 100cc Agilent Cary Eclipse Fluorescence Spectrophotometer 99.999% pure nitrogen at 10 psi Flow rate 0 – 30 L/min High Flow PSA system i-Flow 6010 Agilent Cary 600 Series FTIR spectrometer 10 L/min of clean, dry air or nitrogen < 60 psi Dewpoint: - 70C / -94F Should be CO2 free PG14L Agilent Cary 610/620 FTIR Microscope 10 L/min of clean, dry air or nitrogen <60 psi Dewpoint: - 70C / -94F Should be CO2 free PG14L Agilent MP-AES 10L/min of Nitrogen 99.5% Purity 36.5L.min of Dry Air Genius 3055 Page 6 peakscientific.com Peak Scientific Applications List Application Biotage TURBOVAP LV (CALIPER / ZYMARK) Requirements At 10 psi: 1.7 L/min/ per nozzle At 15/20 psi: 2.83 L/min/ per nozzle Max gas usage for total of 50 nozzles: 140 L/min Inlet gas pressure min. 60 psi/max 100 psi Solution Infinity 90* Infinity 50** At 11 psi: 2.3 L/min/nozzle Inlet gas pressure should be min. 30 psi/max 80 psi Infinity 90** Biotage TURBOVAP 96 Up to 45 L/min at 70psi min Infinity 90* Infinity 50** Range Infinity NM45L Genius 3010 Biotage TURBOVAP 500 No Gas required N/A Biotage ISOLERA ELSD-1080 0.9 L/min to 3.25 L/min @ 60 – 100 psi Infinity NM18L Genius N118LA NG range Zephyr SPE Workstation Clean dry oil-free air or nitrogen at up to 65 psi Infinity 90* Infinity 50** Range Zymark RapidTrace SPE Clean, dry, regulated air or nitrogen Min 10 psi / Max 40 psi Infinity 90* Infinity 50** Range Evaporex /Biotage / Argonaut SPE Dry 96 Dual Microplate Evaporator Concentrator Inlet Gas Pressure: Min 15psi, Max 50psi Gas Flow Min: 25 L/min - Max: 60 L/min Clean, dry, oil-free nitrogen Genius 3010 Infinity 90* Infinity 50** Range Biotage TURBOVAP II (CALIPER / ZYMARK) Page 7 peakscientific.com Peak Scientific Applications List Application Requirements Solution Bruker- MICROTOF Q MICROTOF II (Focus) MICROTOF MAXIS Nitrogen between 5 to 12L/min at 80 -90 psi Aprox 3 to 4L/min is required during standbymode Infinity NM18L Infinity NM32L Genius NM32LA Genius NM3G Bruker Solarix hybrid Qq-FTMS Nitrogen between 5 to 12L/min at 90 psi Aprox 3 to 4L/min is required during standbymode Infinity NM18L Infinity NM32L Genius NM32LA Genius NM3G Bruker MAXIS QQ-TOF MS Nitrogen between 5 to 12L/min at 90 psi Aprox 3 to 4L/min is required during standbymode Infinity NM18L Infinity NM32L Genius NM32LA Genius NM3G Nitrogen between 5 to 15 L/min at 90 psi Aprox 3 to 4L/min is required during standbymode Infinity NM18L Infinity NM32L Genius NM32LA Genius NM3G Bruker AmaZon ion trap MS Nirogen between 5 to 12 L/min at 90 psi Aprox 3 to 4L/min is required during standbymode Infinity NM18L Infinity NM32L Genius NM32LA, Genius NM3G Bruker Fourier Transform Ion Cyclotron Resonance(FT-ICR) mass spectrometer APEX III Nitrogen for both nebulizer gas and dry gas for ESI (Up to 12L/min?) Infinity NM18L Infinity NM32L Genius NM32LA, Genius NM3G Nitrogen up to 32L/min @ 80 psi Clean, Dry Air up to 50 L/min @ 80 psi Genius 3045 Bruker Esquire Ion Trap MS HCT Ion Trap MS Bruker EVOQ TQ LC/MS ELITE + CUBE Bruker APEX IV FTMS Spectrometer Bruker Ultraflex II TOF/TOF Bruker Aurora ICP/MS Varian 820 MS N2 for electrospray ion source: 57 L/min @ 60-100 psi Infinity 5010 Genius-3010 Dry (-40F DP), clean and oil-free air or N2 @ 60-80 psi for pneumatic valve operation Very small quantity, not continuous. Same source as above. (Note: Not Tested UHP Zero Nitrogen at 20 cc/min @ 72 psi Precision Nitrogen Trace 250cc Hydrogen for CRI gas (Collision Reaction Interface) Up to 200 cc/min at 60 psi Typical flow rate: 60 – 90 cc/min Hydrogen for Initialization purge cycle: 2.2 L/min No Peak solution available. Requires hydrogen generator with Buffer tank. Bruker TENSOR series FT-IR Moisture and CO2-free purge gas or nitrogen at 2.5 L/min PG Range Bruker EMX EPR Spectrometer Moisture and O2-free purge gas UHP nitrogen at max 30 L/min i-Flow 6010 NOTE: There are some models of Bruker LCMS that require less than 18 L/min of Nitrogen. Peak Scientific has tested these options and will NOT recommend to connect an N118LA to these applications. The N118LA is not designed to operate continuously at full capacity. Page 8 peakscientific.com Peak Scientific Applications List Application Requirements Nitrogen 99.99% at 150 – 200 psi DIONEX ASE-350 Accelerated Solvent Extractor A source of 99.9% pure nitrogen gas, regulated to between 140 and 200 psi; 150 psi is recommended. UHP gas may be required for apps using a very clean baseline electron capture detector (ECD). Solution Previously: ASE-HP-220 Do not offer this anymore! Requires inert N2 pressure booster Nitrogen 99.99% at 150 – 200 psi DIONEX ASE-350 Accelerated Solvent Extractor A source of 99.9% pure nitrogen gas, regulated to between 140 and 200 psi; 150 psi is recommended. UHP gas may be required for apps using a very clean baseline electron capture detector (ECD). Previously: ASE-HP-220 Do not offer this anymore! Requires inert N2 pressure booster Nitrogen 99.99% at 150 – 200 psi DIONEX ASE-200 Accelerated Solvent Extractor A source of 99.9% pure nitrogen gas, regulated to between 140 and 200 psi; 150 psi is recommended. UHP gas may be required for apps using a very clean baseline electron capture detector (ECD). Previously: ASE-HP-220 Do not offer this anymore! Requires inert N2 pressure booster Nitrogen 99.99% at 150 – 200 psi DIONEX ASE-150 Accelerated Solvent Extractor A source of 99.9% pure nitrogen gas, regulated to between 140 and 200 psi; 150 psi is recommended. UHP gas may be required for apps using a very clean baseline electron capture detector (ECD). Previously: ASE-HP-220 Do not offer this anymore! Requires inert N2 pressure booster DIONEX ESA CORONA CAD Charged Aerosol Detector 99% Nitrogen up to 5 L/min @35 psi Infinity NM18L Genius N118LA NG5000 NG5000A Dionex CORONA ULTRA RS Charged Aerosol Detector Clean, Dry and oil-free air or nitrogen at 60 psi, 4-5 L/min Infinity NM18L Genius N118LA NG5000 NG5000A Nitrogen supply pressure up to 100 psi Operation pressure up to 20 psi Dionex Autotrace 280 SPE Dionex SE400 / SE500 Solvent Evaporators Dionex ICS series Ion Chromatography System 900, 1000, 2000, 3000, 5000 series Only inert gases such as nitrogen, argon, or compressed air (free from oil) should be used for the Dry with Gas step. Nitrogen at 20 psi UHP nitrogen or Helium at 25 – 50 psi for eluent blanketing. Should be CO2-free Infinity 90** Infinity 50** Infinity 90** Infinity 50** Note: PSA nitrogen generators not suitable because they do not remove CO2! Page 9 peakscientific.com Peak Scientific Applications List Application Requirements Solution JASCO J-815 CD Spectrometer Circular Dichroism UHP, Dry Nitrogen up to 3 L/min NG Range JASCO FT/IR-6000 series Purge gas CO2 and H2O-free air Flow rate unknown PG Range Page 10 peakscientific.com Peak Scientific Applications List Application Requirements Solution JEOL LCMS Nitrogen up to 20 L/min Infinity NM32L Genius 1050 Genius NM32LA Genius NM3G Nitrogen up to 10L/min at 100 psi Infinity NM18L Infinity NM32L Genius 1050 Genius NM32LA Genius NM3G AccuTOF™ LC JMS T1000 LC/TOF/MS Page 11 peakscientific.com Peak Scientific Applications List Application Requirements Solution LECO Pegasus 4D GC X GC LN2 Modulators 2 x Cold Jets : N2 at 30 psi with Dewpoint -50°. 6 L/min for each Jet – Total 12 LPM Genius 1052 2 x Hot Jets: clean dry air or N2 at 30 psi with Dewpoint -50°. 20 L/min for each Jet – Total 40 LPM Secondary oven cooling gas: 100 L/min Clean, dry air or N2 at 30 psi (used for 5 mins after each run) N/A* *NOTE: Above LECO Pegasus Modulation gas requirements are as stated in LECO’s manual. However; actual testing carried out by Peak at LECO’s Test-Lab in South Africa confirmed that: 1. 2. Hot Jets actually do not consume more than 10 LPM each Additional 100 L/min of compressed air for oven cooling is not required for operation. It only speeds up the system cool-down time between runs, but has no effect on instrument performance. The system automatically uses the Hot-Jet air/N2 flow for oven cooling when the Hotjets are not in use. The 20 LPM as supplied by the Genius 1052 will suffice for this purpose. Page 12 peakscientific.com Peak Scientific Applications List Application Requirements Solution Perkin Elmer –LCMS range Nitrogen up to 30L/min Infinity NM32L Genius 1050 Genius NM32LA Genius NM3G Perkin Elmer ICP OES Optima 2000 series Optima 2100 DV Optima 3000 series Optima 4000 series Optima 5000 series Optima 7000 DV Optima 7300 V Optima 8000 Optima 8300 Plasma Gas: Argon at 9 L/min Perkin Elmer NexION 300 ICP-MS Purge gas: N2 or Argon Nitrogen purge gas up to 5L/min, 50 psi 99.99% – 99.999% purity (Continuous flow of 1 L/min required) NG5000* NG5000A* NG10L-HP House Air or small oil-free compressor with dryer and filtration. Infinity NM32L Shear gas: Clean, dry and oil-free compressed air or Genius 1050 N2 at 25 L/min , 80 psi Genius NM32LA 14 – 20L min Argon Gas only N/A Perkin Elmer Flexar SQ 300 MS Nitrogen 98% @ 30 L/min 100 psi Infinity NM32L Genius 1050 Genius NM32LA Genius NM3G Perkin Elmer GC Carrier Gas: 60 ml/min @ 90 psi (N2 for packed columns) ECD makeup gas: N2 > 30 ml/min @ 30 psi FID flame gas: H2 > 50 ml/min @ 30 psi FID zero air:: ZA > 500 ml/min @ 30 psi NPD: H2 > 3 ml/min @ 30 psi NPD: ZA > 90 ml/min @ 30 psi Precision Hydrogen Trace Precision Nitrogen 250cc Precision Zero Air 1.5L Fusion 1010 Perkin Elmer Clarus GC 400-480 Perkin Elmer Clarus GC 500 Carrier Gas: H2 or N2 30 ml/min 90 psi FID Air: 450 ml/min @ 30 psi FID H2: 45 ml/min @ 30 psi ECD make up gas: 60 – 100 mL/min The ECD is shipped with charcoal traps (P/N N6600037) installed in the make-up and injector pneumatics lines to remove contamination from the needle valve, flow controller, or pressure regulator. The traps should be replaced periodically Carrier Gas: H2 or N2 30 ml/min 90 psi FID Air: 450 ml/min @ 30 psi FID H2: 45 ml/min @ 30 psi ECD make up gas: 60 – 100 mL/min The ECD is shipped with charcoal traps (P/N N6600037) installed in the make-up and injector pneumatics lines to remove contamination from the needle valve, flow controller, or pressure regulator. The traps should be replaced periodically Precision Hydrogen Trace Precision Nitrogen 250cc Precision Zero Air 1.5L Fusion 1010 Precision Hydrogen Trace Precision Nitrogen 250cc Precision Zero Air 1.5L Fusion 1010 Perkin Elmer Clarus GC 680 Carrier Gas: H2 or N2 30 ml/min 40 psi FID Air: 450 ml/min @ 30 psi FID H2: 45 ml/min @ 30 psi Precision Hydrogen Trace Precision Nitrogen 250cc Precision Zero Air 1.5L Fusion 1010 Perkin Elmer Spectrum 100 Series FT-IR & FT-NIR N2 purge gas flow: 28 L/min at max 100 psi PG28L Perkin Elmer PYRIS DSC & TGA instruments Purge Gas 99.9% + nitrogen up to 65 psi Precision Nitrogen 250cc Page 13 peakscientific.com Peak Scientific Applications List Application Requirements Solution Shimadzu LCMS2010EV Nitrogen at 12 L/min standard rate Nitrogen up to 22.5L/min, 110 psi Infinity NM32L Infinity 5010 Genius NM32LA Genius 1050 Genius 3020 Please note: some Shimadzu LCMS Instruments such as the LCMS2010EV require 110 psi input pressure for optimum operation of the instruments gas flow controllers. 100 – 116 psi range is recommended by Shimadzu as acceptable nitrogen operating pressure, but at the lower end of the scale (100 psi) the flow controls will be slower and less effective, hence the preference for 110 psi. The Genius1022 or the NM32L can be offered for Shimadzu LC/MS; however note that NM32L will need to be connected to an external compressed air supply with a minimum outlet pressure of 125psi in order to increase nitrogen output pressure up to 110psi. Shimadzu LCMS IT TOF Nitrogen up to 21.5L/min Pressure between 100 - 116 psi Shimadzu LCMS-2010A Nitrogen up to 30L/min Pressure between 100 - 116 psi Shimadzu LCMS-2020 Nitrogen up to 21.5 L/min, Pressure between 100 - 116 psi Shimadzu LCMS-8030 Nitrogen up to 25L/min Shimadzu LCMS-8040 Nitrogen up to 25L/min Shimadzu LCMS-8080 Nitrogen at 15 L/min (Curtin & Collision gas) Dry Air (nebuliser gas) at 34 L/min Dry Air (heating gas) 31l L/min Carrier Gas: Shimadzu GC2010 N/A Helium only FID Fuel Gas: Hydrogen FID Air: Zero Air Infinity NM32L Infinity 5010 Genius NM32LA Genius 3020 40ml/ min 400ml/ min Precision Hydrogen 100cc Precision Zero Air 1.5L ECD – nitrogen for Make-up gas Precision Nitrogen Trace 250cc FUSION 1010 Shimadzu GCMS-QP2010 Carrier Gas: Helium only N/A Shimadzu GCMS-TQ8030 Carrier Gas: Helium only N/A Carrier Gas: Helium only N/A Shimadzu GC2014 Shimadzu TOC Vcs/Vcp/Vw/VE Shimadzu TOC-L TOC-L H Type High Sensitivity Total Organic Carbon Analyzer FID Fuel Gas: Hydrogen FID Air: Zero Air 40ml/ min 400ml/ min Precision Hydrogen 100cc Precision Zero Air 1.5L ECD – nitrogen for Make-up gas Precision Nitrogen Trace 250cc FUSION 1010 TOC Gas up to 500cc/min SCTOCA TOC1500HP Ultra Pure Air air at 28-31 psig •CO, CO2, HC content: Each 1 ppm max. •dew point: -50 °C max. SCTOCA / TOC1500HP Gas consumption: (Variable flow rate) 150 mL/min (230 to 250 mL/min during sparging) Page 14 peakscientific.com Peak Scientific Applications List Application Shimadzu TOC-L TOC-L N-Type Regular Sensitivity Total Organic Carbon Analyzer Shimadzu TNM-L Total Nitrogen Analyzer Requirements Solution Ultra Pure Air air at 28-31 psig •CO, CO2, HC content: Each 1 ppm max. •dew point: -50 °C max. Gas consumption: (Variable flow rate) 230 mL/min (A separate 100 mL/min is required for sparging with ASI-L) For Ozone Source Gas: Compressed air /or house air between 28-31 psig and flow at 500mL/min * Can also be ran w/ Ultra Pure Air for TOC-L s SCTOCA / TOC1500HP House air TOC1500 TOC1500HP SCTOCA Shimadzu SSM-5000A Solid Sample Combustion Unit 99.9 % O2 at 500 mL/min High-purity O2 gas is required for high-sensitivity measurement. N/A Shimadzu ELSD LT-II N2 or Air at 3 L/min, max 65 psi Infinity NM18L Genius N118LA NG3000A Shimadzu UV VIS NIR 99.999% nitrogen 50 – 100 L/min High Flow PSA system i-Flow 60** Shimadzu 7510 ICPS Sequential Plasma Emission Spectrometer Argon gas of >99.95% purity at 50 psi Coolant gas 2 to 20 L/min Plasma gas 0.2 to 1.4 L/min Carrier gas 0.1 to 1.5 L/min Purge gas 3.5 L/min N/A Shimadzu IR prestige 21 FTIR Ultra dry CO2-free Air for puge gas PG Range Page 15 peakscientific.com Peak Scientific Applications List Application Requirements Solution Thermo** LTQ Range Nitrogen up to 21 L/min at 100 psi Infinity NM32L Genius NM32LA Genius 1050 Genius NM3G Thermo Quest LCQ DECA XP + Nitrogen up to 30 L/min at 100 psi Infinity NM32L Genius NM32LA Genius 1050 Genius NM3G THERMO LTQ VELOS ETD VELOS PRO ETD LTQ XL ETD Nitrogen up to 15 L/min 100 psi Infinity NM32L Genius NM32LA Genius 1050 Genius NM3G ETD option: 1cc/min 99.999% N2 @ 40 psi (separate bottled gas supply) Nitrogen for the API source and C-Trap: 99.5% pure, flow rate 15 L /min at 100 ± 20 psi THERMO LTQ ORBITRAP VELOS With ETD Module (Electron Transfer Dissociation) Nitrogen supply for ETD option: UHP 99.999% w/less than 1 ppm H2O & O2. Pressure at:40 ±5 psi ETD module consumes less than 1 ATM cc/m of reagent carrier gas, so a standard large bottle (245 ft3) of gas should last approximately two years. Thermo LTQ Series LTQ XL / LTQ Orbitrap Velos Infinity NM32L Genius NM32LA Genius 1050 Genius NM3G (separate bottled gas supply) NG Range is only a cost- effective option if UHP N2 is used for other apps, besides just ETD. Nitrogen up to 21 L/min 100 psi Infinity NM32L Genius NM32LA Genius 1050 Genius NM3G Thermo Finnigan TSQ Quantum TSQ Quantum Ultra TSQ Quantum Discovery Nitrogen up to 21 L/min at 100 psi Infinity NM32L Genius NM32LA Genius 1050 Genius NM3G Thermo Exactive Nitrogen up to 30 L/min at 100 psi +- 10 psi NM32L, NM32LA, NM3G Thermo Q- Exactive Thermo Q- Exactive Plus Nitrogen up to 30 L/min at 116 psi Genius 1022 Thermo LXQ Linear Nitrogen up to 15 L/min, up to 100psi Infinity NM32L Genius NM32LA Genius 1050 Genius NM3G Thermo LCMS with HESI or HESI-II probe attachment. Typical settings, as recommended by Thermo do not exceed 25 L/min, however, at max gas settings with HESI probe, nitrogen flow requirement was measured up to 40L/min, 100 psi. Thermo/ Dionex*** MSQ+ Nitrogen up to 15L/min, 100 psi Infinty NM18L Genius N118LA Genius N418LA Argon Torch Gas at 15 L/min N/A Nitrogen Purge gas at 10 L/min, 80 psi 99.995% purity NG10L-HP Thermo iCAP 6500 Please note; Thermo state that these settings are unlikely to be used for real analysis as they are not optimum and would degrade the signal for any given sample. Verify with customer. Infinity NM32L Infinity NM45L Infinity 5010 Genius NM32LA Genius 1050 Genius NM3G Page 16 peakscientific.com Peak Scientific Applications List Thermo Nicolet 380 FTIR 4700 FTIR 5700 FTIR 6700 FTIR 8700 FTIR Standard: Purge gas 14 L/min at 40 psi Thermo Nicolet NEXUS 870 FTIR Thermo Nicolet NEXUS 670 FTIR Standard: Purge gas 14 L/min at 40 psi Instrument with Smart Purge option: Purge gas up to 50L/min at 80 psi Instrument with Smart Purge option: Purge gas up to 50L/min at 80 psi PG Range PG Range Standard: Purge gas 7 L/min at 20 psi Thermo Nicolet AVATAR FTIR Thermo Nicolet 560 Magna FTIR Thermo Nicolet 760 Magna FTIR Thermo Nicolet iS50 FT-IR Spectrometer Thermo 51i Total HydroCarbon Analyzer with FID Thermo 55C Hydrocarbon Analyzer Instrument with Smart Purge option: Purge gas up to 50L/min at 80 psi PG Range Standard: Purge gas 12 L/min at 40 psi Instrument with Smart Purge option: Purge gas up to 50L/min at 80 psi PG Range Standard: Purge gas 14L/min at 40 psi Instrument with Smart Purge option: Purge gas up to 50L/min at 80psi Makeup Air Flow Rate: 150 - 300 cc/min Hydrocarbon free air PG Range Precision Zero Air 1.5L Fuel Flow Rate: 10 to 35 cc/min Hydrogen Support Gas Pressures Zero Air: 20 - 30 PSI Hydrogen: 20 - 30 PSI (hydrogen) UHP Zero Nitrogen: 25 - 35 PSI HC Free Air (200 - 300 cc/min) N2 carrier (35 cc/min) H2 Fuel (25 cc/min) Fusion 1010 Precision Nitrogen Trace 250cc Precision Zero Air 1.5L Note: additional pressure regulator required! Carrier gas:He, H2 or N2 Capillary Column: Typically 2ml/min Packed Column: 40ml.min Thermo Trace GC/FID Make up gas:N2 or He 10-60ml.min / Typically 30ml.min (only used with capillary columns) Detector gas: H2 for fuel gas 30-50ml/min ZA for flame support 300-600ml/min Precision Hydrogen Trace Precision Nitrogen Trace 600cc Precision Zero Air 1.5L *** Thermo/ Dionex MSQ: • N118LA model number is used for general sales • N418LA model number is ONLY used for direct sales to Thermo/ Dionex. • N418LA is rebadged by Dionex to MSQ18LA Page 17 peakscientific.com Peak Scientific Applications List Application Requirements Solution Varian 300 MS (GC/MS/MS) Helium only for carrier gas N/A Varian 320 MS (GC/MS) Helium only for carrier gas N/A Varian 310 MS (LC/MS) Nitrogen 12 L/min 80 psi Air 2 L/min 80 psi Genius 1053 Precision Zero Air 3.5L Varian 320 MS (LC/MS) Nitrogen 12 L/min 80 psi Air 2 L/min 80 psi Genius 1053 Precision Zero Air 3.5L Varian 325 MS (LC/MS) Nitrogen 12 L/min 80 psi Air 2 L/min 80 psi Genius 1053 Precision Zero Air 3.5L Varian 500 MS (LC Ion Trap) Nitrogen 12 L/min 80 psi Air 2 L/min 80 psi (APCI requires additional 5 L/min of nitrogen) Genius 1053 Precision Zero Air 3.5L Varian NMR 60 – 500L/min of clean dry air or nitrogen Minimum Dewpoint: -40C / - 40 F AD range Infinity 50** FID Detector: Zero Air up to 500 cc/min Hydrogen up to 50 cc/min Make up gas up to 50 cc/min Precision Zero AIr 1.5L Varian GC 3900 TCD Detector: Make up gas and reference gas up to 50 cc/min (Helium or Nitrogen) Precision Nitrogen 250CC Precision Nitrogen Trace 250CC Page 18 peakscientific.com Peak Scientific Applications List Application Requirements Solution Waters LCT Premier Range Nitrogen up to 30L/min, 100psi Infinty NM32L Genius NM32LA Genius 1050 Genius NM3G Waters ZQ /EMD Nitrogen up to 30 L/min, 100 psi Infinty NM32L Genius NM32LA Genius 1050 Genius NM3G Nitrogen up to 30L/min, 100psi Infinty NM32L Genius NM32LA Genius 1050 Genius NM3G Waters 3100 Mass Detector Nitrogen up to 30L/min, 100psi Infinty NM32L Genius NM32LA Genius 1050 Genius NM3G Waters LCT Premier Range Nitrogen up to 30L/min, 100psi Infinty NM32L Genius NM32LA Genius 1050 Genius NM3G Nitrogen up to 30 L/min, 100 psi Infinty NM32L Genius NM32LA Genius 1050 Genius NM3G Waters Acquity Range Nitrogen up to 30L/min, 100psi Infinty NM32L Genius NM32LA Genius 1050 Genius NM3G Waters 3100 Mass Detector Nitrogen up to 30L/min, 100psi Infinty NM32L Genius NM32LA Genius 1050 Genius NM3G Waters Acquity Range Waters ZQ /EMD API gas: Nitrogen up to 20L/min, 100psi Waters Synapt Range Synapt G2 HDMS also requires IMS gas: >99.5% N2 @ 14 psi, 10 – 100 ml/min *Common gas supply for API & IMS is ok if higher purity is used. Infinty NM32L Genius NM32LA Genius 1050 Genius NM3G Recommendation TBA* Waters XEVO TQS Nitrogen at 23.5 L/min, 100psi Infinty NM32L Genius NM32LA Genius 1050 Genius NM3G Waters G2 QTOF Nitrogen at 23.5 L/min, 100 psi Infinity NM32L Genius 1050 Genius NM32lA Genius NM3G Waters QTOF API-US API-LC/MS/MS Desolcation Gas: N2 @ 3 L/min - 12.5 L/min Cone Gas: N2 @ 1 L/min - 5 L/min Nebulising Gas: 0.3 L/min Max Total N2 18 L/min at 90-100 psi Infinity NM32L Genius 1050 Genius NM32LA Genius NM3G Page 19 peakscientific.com Peak Scientific Applications List Application Requirements Solution Waters QTOF API-US API-LC/MS/MS Desolvation Gas: N2 @ 3 L/min - 12.5 L/min Cone gas: N2 @ 1 L/min – 5 L/min Nebulising gas: 0.3 L/min Max Total N2: 18 L/min at 90 – 100 psi Infinty NM32L Genius NM32LA Genius 1050 Genius NM3G Nitrogen up to 20L/min, 100psi Infinty NM32L Genius NM32LA Genius 1050 Genius NM3G Waters Quattro Premier Nitrogen up to 25L/min, 100psi Infinty NM32L Genius NM32LA Genius 1050 Genius NM3G Waters Quattro Micro GC/MS/MS Nitrogen up to 20L/min, 100psi Infinty NM32L Genius NM32LA Genius 1050 Genius NM3G Waters ELSD 2420 Nitrogen up to 4 L/min @ min 65 psi Infinity NM18L NG4000A Genius N118LA Waters ELSD 2424 Nitrogen up to 4 L/min @ min 65 psi Infinity NM18L NG4000A Genius N118LA Waters QTOF Micro Nitrogen between 10 – 20 L/min, 100psi Infinty NM32L Genius NM32LA Genius 1050 Genius NM3G Waters APGC/XEVO TQ MSD Atmospheric Pressure GC/ MS/MS Carrier Gas: He 2.0 mL/min Make-up Gas: N2 350 mL/min N/A Precision Nitrogen 600cc Waters APGC/XEVO QTOF Atmospheric Pressure GC/ MS-TOF Carrier Gas: He 1.2 mL/min Make-up Gas: N2 250 mL/min N/A Prcision Nitrogen 250cc Nitrogen up to 20 L/min @ min 98 psi Infinity NM32L Genius 1050 Genius NM32LA Genius NM3G Waters Quattro Micro LC/MS/MS Waters QDa Page 20 peakscientific.com Peak Scientific Applications List Other Applications Application Requirements DESI- OmniSpray ion Source Desorption Electrospray Ionization (DESI) DESI ion Source requires high velocity nitrogen spray at 150 psi for optimum results. (Prosolia.com) This pressure is currently not obtainable with gas generator solution. Solution N/A FT-IR Fourier Transform – Infra Red Spectroscopy CO2-free Dry Air or N2 10 – 100 L/min PG Range NDIR CO2-free Air or N2 10 – 100 L/min PG Range ICP UHP N2 for Purge up to 10 L/min NG Range NG10L-HP, NG5000A* Teledyne Leeman labs Prodigy H ICP Spectrometer UHP N2, up to 2 L/min @ 20 psi NG Range Teledyne Leeman labs Profile ICP Spectrometer UHP N2 for Optics purge 10 L/min @ 60 – 90 psi NG Range NG10L-HP NG5000A* Dry Air or N2 for lifting/spinning /Ejecting N2 for magnet cooling NMR 200 – 600 MHz or Solid State AA Atomic Absorption Spectroscopy Minimum Dewpoint: -40C / - 40 F Flow rates vary: 45 – 300 L/min Dry Air for oxidant gas 20 – 200 L/min AD Range Infinity 50** AD Range Dry compressed air or nitrogen, 80-100 psi Eksigent 1D Nano LC (Pressure in the system is generated by connecting laboratory air or nitrogen to a pneumatic pressure amplifier. The MFC controller regulates this pressure to generate the required flow rate.) TBA UV Fluo UHP N2 for purge gas 1 – 5 L/min NG Range TOC Analyzer TOC Gas up to 1200 cc/min TOC1500 TOC1500HP SCTOCA Sedere SEDEX LT ELSD LT- ELSD 65, 75 & 85 Up to 3.5 L/min at 50 psi (N2 or AIR) Infinity NM18L Genius N118LA NG range, Precision Zero Air 3.5L Polymer Laboratories ELSD PL-ELS 2100 Ice PL-ELS 1000 PL-EMD 960 Nitrogen (98% purity or better) Up to 3.5L/min at 60 psi - max 100 psi Infinity NM18L Genius N118LA Precision Zero AIr 3.5L Microsaic 3500 MiD Pressure 2- 6 bar (29 – 87 psi) Purity (99.5%) Flow rate TBA Teledyne Fusion TOC TOC gas up to 500cc/min at 65 to 100 psi Hydrocarbon and Carbon Dioxide (CO2 ) free air with TOC content <1ppm TOC1500 TOC1500HP SCTOCA Teledyne Torch TOC TOC gas up to 500cc/min at 65 to 100 psi Hydrocarbon and Carbon Dioxide (CO2 ) free air with TOC content <1ppm TOC1500 TOC1500HP SCTOCA OI Analytical Aurora TOC Range TOC gas up to 500cc/min at 65 to 100 psi Hydrocarbon and Carbon Dioxide (CO2 ) free air with TOC content <1ppm TOC1500 TOC1500HP SCTOCA OI Analytical Aurora TOC Range TOC gas up to 700 cc/min at 50 -60 psi TOC1500 TOC1500HP Page 21 peakscientific.com Peak Scientific Applications List Other Applications Application Requirements Solution OI Analytical TOC 9210 Range TOC gas up to 200 cc/min at 50 -60 psi TOC1500 TOC1500HP SCTOCA OI Analytical 5380 PFPD Carrier Gas: N2, or H2 up to 10ml/min 80 psi FPD Zero Air : 20 – 30 ml/min at 60 psi FPD Hydrogen up to 15 ml/min at 60 psi Precision Nitrogen Trace 250cc Precision Hydrogen Trace Precision Zero Air 1.5L OI Analytical 4450 Tandem PID/FID PID Sweep Gas H2 up to 40 ml/min PID Make-up gas N2 up to 25 ml/min FID Air ZA up to 200 ml/min Precision Nitrogen Trace 250cc Precision Hydrogen Trace Precision Zero Air 1.5L Fusion 1010 ALPHA MOS / FOX Electronic Nose TOC gas at minimum 150 ml/min @ 30 psi SC-TOC-A TOC1500HP IonSense DART ion source Helium, or 99.998% nitrogen at 80 psi. Flow rate: 2.5 L/min up to 7 L/min NG Range NG10L-HP Pulsed Flame Photometric Detector Total UHP nitrogen flow: 5 L/min. 3 separate flow controlled inlets: Chirascan™ Circular Dichroism Spectrometer • • • lamp housing : 1 l/min monochromator : 3 l/min light path sample chamber : 1 l/min NG5000 NG5000A, NG10LHP UHP dry nitrogen at 20 - 35 psi Flow rate between 10 – 20 L/min AVIV Circular Dichroism Spectrometer Model 62 DS Model 202 Model 420 Note: Oxygen will damage the lamp and optics of the CD Spectrometer. If nitrogen supply runs out during experiment, it will permanently damage the instrument. NG10L-HP with N2 buffer tank iFlow 6010 A buffer/storage tank downstream of the generator, or a back-up bottled gas supply with automatic switch-over is recommended. Nitrogen supply should be fitted with an additional 5 ppb O2 scrubber and indicating trap. (99.9999995% pure nitrogen) SRI Instruments GC FPD H2: 60mL/min (30 mL/min for each of the two hydrogen flows) Precision Hydrogen Trace FPD air: 100mL/min Precision Zero Air 1.5L Total Hydrocarbon Analyzer (THA) with FID detector Hydrogen for Fuel Gas (up to 50cc/min) Zero Air for flame support (up to 500 cc/min) Precision Hydrogen 100cc Precision Zero Air 1.5L ESA CORONA CAD detector 99% Nitrogen up to 5 L/min @35 psi (or clean dry oil-free air) Infinity NM18L NG5000 NM500A ALLTECH ELSD 800 Up to 3L/min 98-99% pure Nitrogen at 15-90 psi Infinty N0M18L Genius N118LA NG3000A ALLTECH ELSD 3300 Up to 4L/min 98-99% pure Nitrogen at 60-80 psi Infinty N0M18L Genius N118LA NG4000A CO² Analyzer Calibration Gas up to 1000 cc/min CO²-free /Hydrocarbon-free gas CG15L TOD (Total Oxygen Demand Analyzer) UHP Zero grade nitrogen up to 300cc/min Fusion 1010 Entech Canister Cleaner Zero Air at 50 psi CG range Page 22 peakscientific.com Peak Scientific Applications List Other Applications Application Buck Sci. 210/211 VGP Atomic Absorption Spectrophotometer Requirements Solution Acetelyne or Hydrogen at 15 L/min N/A Clean Dry, Oil Free Air: 20L/min @ 50 psi Nitrogen UHP. Flow rate varies depending on application TBA Cyclic Voltammetry (CV) UHP Nitrogen for cell purge and blanketing 5 – 10 cc/min Precision Nitrogen Trace 250cc Rheometry Clean, dry, oil-free compressed air or nitrogen at 50 -60 psi. Flow rate: 30 to 60 L/min Genius 3010 Infinity 9010 Infinity 5010 TA Instruments Rheology Q series UHP nitrogen up to 100 cc/min at 10-20 psi Precision Nitrogen Trace 250cc TA Instruments Q5000 SA Dynamic Vapor Sorption TA Instruments Autosampler Q5000IR TGA TA Instruments Q50 / Q500 TGA Thermogravimetric Analyzer With Standard Furnace TA Instruments Q50 / Q500 TGA Thermogravimetric Analyzer With EGA Furnace TA Instruments Q600 SDT Slimultaneous TGA/DSC Analyser TA Instruments Qseries DSC Differential Scanning Calorimeter Titration Oven (Coulometric Karl Fisher) GEA NIRO SD Micro Spray Dryer GEA NIRO PSD-1 Spray Dryer Purge Gas (Dry UHP N2) for humidity chamber: 200 ml/min at 10-20 psi Precision Nitrogen Trace 250cc Purge Gas for furnace/sample area: 10 ml/min at 10-20 psi Purge Gas (UHP N2) for balance area: 10 ml/min at 10-20 psi Purge Gas for furnace/sample area: 25 ml/min at 10-20 psi Precision Nitrogen Trace 250cc Purge Gas (UHP N2) for balance area: 40 ml/min at 10-20 psi Purge Gas for furnace/sample area: 60 ml/min at 10-20 psi Precision Nitrogen Trace 250cc Purge Gas (UHP N2) for balance area: 10 ml/min at 10-20 psi Purge Gas for furnace/sample area: 90 ml/min at 10-20 psi Purge Gas (UHP N2) flow: 100ml/min Precision Nitrogen Trace 250cc Precision Nitrogen Trace 250cc Purge Gas (UHP N2) for balance area: 10 ml/min at 10-20 psi Purge Gas for furnace/sample area: 90 ml/min at 10-20 psi 0.1 – 0.5 L/min clean, dry N2 Liquid N2 at 30 Kg/hour Or Gaseous N2 at 15 – 20 cfm / 280 – 560 LPM Pressure: 80 to 90 psi Liquid N2 at 80 Kg/hour Or Gaseous N2 at 35 – 40 cfm / 990 – 1100 LPM Pressure: 80 to 90 psi Precision Nitrogen Trace 250cc Precision Nitrogen Trace 250cc Infinity 50** Infinity 90** Infinity 50** Infinity 90** Page 23 peakscientific.com Peak Scientific Applications List Other Applications Application Requirements Solution Spray Gas Compressed Air or Nitroigen 200-1000 L/hr, 5-8 bar (4-17 L/min at 70-116 psi) BUCHI Mini Spray Dryer B-290 Note: The Inert Loop B-295 attachment used for organic solvents has an 02 analyzer that shuts down the system when it detects 6% Oxygen in the Nitrogen. Infinity NM32L Infinity NM45L Genius NM32LA Genius 1050 Ensure membrane Nitrogen system is used that can achieve 98%-99% consistently to avoid shut-downs during runs Alcor Micro Carbon Residue Tester Clean Dry CO2-free UHP Nitrogen 20-145 psig); cylinder preferred, consumption approx 37L 91.3ft3 per 95 minute test Note: No available unit with CO2 removal CEM Microwave Peptide Synthesizer Requires UHP Nitrogen at 20 L/min at 25 psi i-flow 6010 Applied Biosystems 433A peptide Synthesizer Requires UHP Nitrogen at 65 psi NG Range i-Flow 6010 Perseptive Biosystems / Applied Biosystems MAriner Biospectrometry Workstation UHP (4.8 grade purity) Nitrogen at 80-0100 psi Nebulizer gas flow: 0.2-1 L/min Curtain gas flow: 0.5 -2 L/min Auxiliary gas flow: 3 -8 L/min NG Range Page 24 peakscientific.com Peak Scientific Applications List Sample Evaporation /Concentration/ SPE Application Requirements Solution Sample Prep Solvent Evaporat Nitrogen 95% - 99% up to 140 L/min Infinity 90** Infinity 50** Organomation Aquavap Nitrogen evaporator Nitrogen flow rate up to 4 L/min 6 positions X 660 ml/position/minute Infinity NM18L Genius N118LA Infinity 90** Organomation N-EVAP Max Nitrogen consumption at 30 psi: 6 -12 positions = up to 5 LPM 24 positions = up to 10 LPM 34 – 45 positions = up to 20 LPM Infinity 90** Infinity 50** Organomation MULTIVAP Max Nitrogen consumption at 30 psi: 64 -100 positions = up to 30 LPM 96 positions = up to 15 LPM 30 positions = up to 15 LPM 48 positions = up to 20 LPM Genius NM32LA Genius 1050 Infinity 90** Infinity NM32L Organomation MICROVAP Max Nitrogen consumption at 30 psi: up to 30 LPM Genius NM32LA Genius 1050 Infinity 90** Infinity NM32L SPEware System 96 Positive Pressure Manifold Cerex SPEware 48 port Processor & Evaporator Moisture, Hydrocarbon and particulate-free gas at 40 – 50 psi ( N2 or CDA) Average Flow rate: 3 L/min Adjustable up to 10 L/min Infinity 90** Infinity NM18L Genius N118LA Processor: N2 at 2 cfh (1 L/min) 70 psi Evaporator: N2 at 25 cfh (12 L/min) 70 psi Infinity 90** Infinity NM18L Genius N118LA SPEware Cerex 96 port Processor & Evaporator Processor: N2 at 2 cfh (1 L/min) 70 psi Evaporator: N2 at 25 cfh (12 L/min) 70 psi Infinity 90** Infinity NM18L Genius N118LA Evaporex / Biotage / Argonaut SPE Dry 96 Dual Microplate Evaporator Concentrator 8 place RapidVap N2: 17 L/min 20psi max RapidVap N2/48: 99 L/min 20psi max Genius 3010 Infinity 90** Infinity50** 8 place RapidVap N2: 17 L /min 20 psi max RapidVap N2/48: 99 L /min 20 psi max Infinity 90** Infinity 50** LABCONCO RapidVap VACUUM N2 & N2/48 LABCONCO RapidVap VERTEX 73200 Series Max N2 demand for all 50 nozzles: 6.5 cfm / 185 LPM nitrogen at 50 psi 5 cfm / 142 LPM nitrogen at 24 psi 4.5 cfm / 127 LPM nitrogen at 20 psi N2 consumption = 1 cfm (28.3 L/min) per row of 10 nozzles at 24 PSI. Infinity 90** Infinity 50** N2 consumption = 0.74 cfm (21 L/min) per row of 10 nozzles at 15 PSI. Gilson SPE 215 Nitrogen up to 20 L/min Genius NM32LA Genius 1050 Infinity 90** Infinity 50** TECHNE Sample Concentrator Nitrogen 99% 7 - 15 L/min at max 2 psi Infinity NM18L Genius NM32LA Apricot Evaporex EVX96 Nitrogen up to 60 L/min at 15 – 50 psi Infinity 90** Infinity 50** Genius 3010 Porvair Sciences UltraVap RC + Minivap Nitrogen min 28 L/min - MAx 80 L/min @ max 6 bar / 87 psi Infinity 90** Infinity 50** Genius 3010 Page 25 peakscientific.com Peak Scientific Applications List Sample Evaporation /Concentration/ SPE cont. Zymark RapidTrace SPE Clean, dry, regulated air or nitrogen Min 10 psi / Max 40 psi UCT Positive Pressure Manifold SPE N2 or Compressed Air at 80 – 120 psi Biotage TURBOVAP LV (CALIPER / ZYMARK) Infinity 90** Infinity 50** Infinity 90** Infinity 50** At 10 psi: 1.7 L/min/ per nozzle At 15/20 psi: 2.83 L/min/ per nozzle Max gas usage for total 50 nozzles: 140 L/min Inlet gas pressure min. 60 psi/max 100 psi Infinity 90** Infinity 50** Biotage TURBOVAP II (CALIPER / ZYMARK) At 11 psi: 2.3 L/min/nozzle Infinity 90** Infinity NM32LA Genius 1050 Biotage TURBOVAP 96 Up to 45 L/min at 70psi min Infinity 90** Infinity NM45L Genius 3010 Biotage TURBOVAP 500 No Gas required N/A Biotage ISOLERA ELSD-1080 0.9 L/min to 3.25 L/min @ 60 – 100 psi Infinity NM18L Genius N118LA NG Range Zephyr SPE Workstation Clean dry oil-free air or nitrogen up to 65 psi Infinity 90** Infinity 50** Zymark RapidTrace SPE Clean, dry, regulated air or nitrogen Min 10 psi / Max 40 psi Infinity 90** Infinity 50** Nitrogen up to 30 L/min at 15 – 20 psi Genius NM32LA Genius 1050 Infinity 90** Infinity 50** Genevac EZ-2 Evaporator Inlet gas pressure should be min. 30 psi/max 80 psi NOTE: When sizing a nitrogen generator to supply both Mass spec instruments as well as Evaporation equipment, please keep in mind that in some cases, with high N2 demand evaporators, it may be more efficient and cost effective to offer a separate NM**L and CNMG**L generator. If you are adding the evaporator flow rate to the NM**L capacity, also consider the added purge loss through the membranes. (Purge loss = 120 L/min per membrane) In the majority of labs, Evaporation equipment is only used intermittently, not continuous. With a separate CNMG**L generator, the unit will only have a demand for compressed air when the evaporator is in operation, therewith preventing waste of compressed air. Furthermore, as the CNMG unit has fewer membranes than the NM**L equivalent, it has a significantly lower purge loss, and therefore places less demand on compressed air supply. Ultimately this will result in more efficient and cost effective operation of the existing compressed air system, or it may enable you to offer a smaller capacity compressor for labs with no house-air supply. Page 26 peakscientific.com Peak Scientific Applications List MULTIPLE LCMS INSTRUMENTS LCMS Manufacturer Number of Instruments Solution AB SCIEX up to 2 Infinity 1032, Genius 3030 AB SCIEX up to 3 Infinity 1033 AB SCIEX up to 4 Infinity1034 AB SCIEX + OTHER 1 x standard API + 1 x standard LCMS Genius 3040 AB SCIEX 6500 + 550 w/Selexion Up to 2 Up to 3 Up to 4 Infinity 1032 Infinity 1033 Infinity 1034 THERMO Q Exactive Up to 2 ( 116 psi) Infinity 5010 / Genius 3022 Shimadzu LCMS2010 Up to 2 (110 psi Infinity 5010 / Genius 3022 AGILENT CHIP CUBE Up to 2 (Not for iFunnel models!) Genius 3023 Low Flow LC/MS 2 x @max 15 L/min each Infinity NM32L Low Flow LC/MS 2 x @max 20 L/min each Infinity NM45L ALL OTHERS * up to 2 @ max 30 Lpm each Infinity 5010 Genius 3020 ALL OTHERS * up to 4 @ max Lpm each Infinity 5020 ALL OTHERS * up to 6 @ max 30 Lpm each Infinity 5030 ALL OTHERS * up to 8 @ max 30 Lpm each Infinity 5040 ALL OTHERS * up to 10 @ max 30 Lpm each Infinity 5050 ALL OTHERS * up to 12 @ max 30 Lpm each Infinity 5060 ALL OTHERS * up to 14 @ max 30 Lpm each Infinity 5070 ALL OTHERS * up to 16 @ max 30 Lpm each Infinity 5080 • For the Infinity 1034, it may be possible to supply more than 4 x API instruments at the same time, as long as the total curtain, source and exhaust gas requirements do not exceed the total capacity of the generator. • Standard LCMS (ALL OTHERS) are calculated on a maximum requirement of 30L/ min for each instrument. When sizing an Infinity 50** system for a variety of instruments with different gas requirements, please add up the total gas requirement for each of the instruments. It is recommended to leave sufficient margin when sizing the generator to make sure the generator does not continuously run at its maximum flow capacity and leave some margin for potential future additions. • For multi-instrument set-ups including Evaporator equipment, please see comments on previous page. Page 27 peakscientific.com Peak Scientific Applications List GAS CHROMATOGRAPHY GENERAL APPLICATIONS NOTES: • Capillary and packed column carrier gas flow rates are variable for all GC’s, mainly depending on the column size, the type of gas and the type of injector. • The standard flow rates are shown below, but please note that for SPLIT/SPLITLESS INJECTORS carrier gas flow rates have to be individually calculated based on the operators’ maximum Split Flow Rate. Split Vent flow rates up to 500 cc/min are possible, therefore the hydrogen generator should be appropriately sized. PLEASE ASK CUSTOMERS FOR MAX SPLIT FLOW RATE AND ADD 20CC TO THAT FLOW TO GET TOTAL CARRIER GAS USE! • When calculating total required gas flows, take into account that clients may be operating dual columns in the GC-oven, and each GC instrument can have up to three detectors. • Hydrogen can often be used as an alternative to Helium as carrier gas in most GC’s, depending on the detector and the specimen to be analyzed. For Carrier Gas, hydrogen has to be very dry, therefore the Precision Hydrogen Trace is the preferred generator model. PH range generators should only be used with an additional moisture trap in the gas stream. • Hydrogen may react with compounds in the specimen that are in the same thermal conductivity range as hydrogen. ‘False peaks’ a.k.a. ‘ghost peaks’ will occur on the Chromatogram. Also, a chromatographer has to select the correct column stationary phase for use with hydrogen, as the gas may react with components in certain types of stationary phase, which will ‘look like’ hydrocarbon contamination. • • Trace levels of moisture and oxygen in the carrier gas decompose the stationary phase inside the GC-column, therefore; all GC manufacturers recommend installing Moisture, Oxygen and Hydrocarbon traps on any GC gas line, even if the source gas is already very pure. In the event of a leak occurring on the gas supply line, the traps will ensure that ambient contaminants do not enter the system. • • > A hydrocarbon-trap does NOT remove methane from nitrogen carrier/make-up gas. For total hydrocarbonfree nitrogen you need a catalyst to remove CH4 (Methane). The Peak Zero-Air and Zero-Nitrogen generators feature a catalyst. • Stainless steel or copper tubing is recommended for GC gases. Do not use nylon or Teflon/PTFE tubing as they are permeable and allow impurities to enter the gas stream. • For combination N2/Air generators; only offer ANG-range generators if clean, dry air is sufficient. For GC applications that require Zero Air (Hydrocarbon-Free Air) in combination with Zero Nitrogen, please offer the Fusion 1010 model, or separate ZA + NG units, as the ANG-range does not produce zero air • Gas purity requirements for GC vary depending on the ‘limit of detection’ the chromatographer is looking for. In the case of Trace-detection (0-1 ppm) only Ultra pure/ Research grade gases should be used (99.9995% 99.9999%) Page 28 peakscientific.com Peak Scientific Applications List GAS CHROMATOGRAPHY GENERAL APPLICATION cont. INSTRUMENT GAS REQUIREMENT GENERATOR GC/FID Hydrogen for fuel gas (up to 50 cc/min per FID) Zero Air for flame support gas (up to 600cc/min per FID) Hydrogen for capillary column carrier gas (up to 10cc/min) Nitrogen for packed column carrier gas (up to 60 cc/min) Precision Hydrogen Trace Precision Nitrogen Trace 250cc Precision Zero Air 1.5L TOTAL FID Hydrogen for fuel gas (up to 50cc/min) Zero Air for flame support gas (up to 500cc/min per FID) Precision Zero Air 1.5L GC/FPD Hydrogen for capillary column carrier gas (up to 50cc/min) Nitrogen for packed column carrier gas (up to 50 cc/min) Hydrogen for fuel gas (up to 90 cc/min) Zero Air for flame support gas (up to 200cc/min) Precision Hydrogen Trace Precision Nitrogen Trace 250cc Precision Zero AIr 1.5L Fusion 1010 GC/NPD Hydrogen for capillary gas (up to 50 cc/min) Nitrogen for make up gas (up to 30 cc/min) Zero Air for flame support gas (up to 200cc/min) Precision Hydrogen Trace Precision Nitrogen 250cc Precision Zero AIr 1.5L Fusion 1010 GC/ECD UHP Hydrogen, Zero Nitrogen or Argon / CH4 for Carrier Gas: Capillary Column flow: up to 20 cc /min Packed Colimn flow: up to 60 cc/ min Precision Hydrogen Trace Precision Nitrogen Trace 250cc GC/TCD H2 or N2 as carrier/reference gas (up to 50 cc/min) Precision Hydrogen Trace Precision Nitrogen Trace 250cc GC/ATD Dry air purge PG Range GC/AED UHP Zero Grade Nitrogen for carrier gas (up to 1L/min) GC/ELCD Hydrogen as reaction gas (up to 200 cc/min) GC/HALL Hydrogen as reaction gas (up to 200 cc/min) GC/PID Hydrogen for Carrier Gas (up to 60 cc/min) GC/MS Carrier Gases: Capillary column ; Hydrogen up to 10 cc/min Packed column: Nitrogen up to 40 cc/min Thermo 55C Hydrocarbon Analyzer (DP -70°C ) ( up to 2 L/min) Support Gas Pressures Zero Air: 20 - 30 PSI Hydrogen: 20 - 30 PSI (hydrogen) UHP Nitrogen: 25 - 35 PSI HC Free Air (200 - 300 cc/min) Zero N2 carrier (35 cc/min) H2 Fuel (25 cc/min) Note: additional pressure regulator required! Perkin Elmer Clarus SQ8 GCMS Carrier Gas 5 ml/min Helium or Hydrogen Oi Analytical Tandem PID/FID Detector for GC PID Sweep gas - Hydrogen 99.999% up to 40 cc/min PID Make up gas – N2 99.999% or Helium at 25 cc/min FID Air – Zero Air up to 200 cc/min SIS TD 5 Short Path Thermal Desorption Carrier Gas Helium or Nitrogen Max 100 c/min at 40 – 60 psi Compressed Air for gas valve operation, 100 cc/min at 60 psi N/A Precision Hydrogen Trace Precision Nitrogen Trace 250cc Precision Hydrogen Trace Precision Nitrogen Trace 250cc Precision Zero AIr 1.5L Precision Hydrogen Trace Precision Nitrogen 250cc Precision Zero AIr 1.5L Precision Nitrogen Trace 250cc Precision Zero AIr 1.5L Fusion 1010 Page 29 peakscientific.com Peak Scientific UK Fountain Crescent Inchinnan Business Park Inchinnan PA4 9RE Scotland, UK Peak Scientific North America 19 Sterling Road Suite #1 Billerica, MA 01862 USA Peak Scientific India 202, Amsri Shamira Old Lancer Line Opp. St. Mary’s Degree College S.D. 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