Supplementary material for:
Phosphorus Binding to Nanoparticles and Colloids in Forest Stream Waters
N. Gottselig1*, V. Nischwitz2, T. Meyn3, W. Amelung1,4, R. Bol1, C. Halle3, H. Vereecken1, J.
Siemens4, 5, E. Klumpp1
1
Institute of Bio- and Geosciences, Agrosphere (IBG-3), Forschungszentrum Jülich GmbH,
Jülich, Germany
2
Central Institute for Engineering, Electronics and Analytics, Analytics (ZEA-3),
Forschungszentrum Jülich GmbH, Jülich, Germany
3
Department of Hydraulic and Environmental Engineering, Norwegian University of Science
and Technology, Trondheim, Norway
4
INRES Soil Science and Soil Ecology, University of Bonn, Bonn, Germany
5
Institute of Soil Science and Soil Conservation, Justus Liebig University Giessen, Giessen,
Germany
Pages: 5
Tables: 2
Figures: 2
1
Table S1: Stream water parameters at catchment outlet points of the sampling sites. T = stream
water temperature [°C], cond. = electrical conductivity [µS cm-1]. CON = Conventwald, LEI = Leirerva,
MIT = Mitterfels, WES = Vessertal, W = Wüstebach
2
Table S2: Organic C, Al, Si, Mn, Fe results: Total sample concentrations and percentage of elemental binding in
the nanoparticulate and fine colloidal fractions over all stream points per site. n = 29
Leirelva
Wüstebach
Vessertal
Mitterfels
Conventwald
Organic C
Total
samples
[%]
[mg/L]
Al
Si
Mn
Total
Total
Total
[%]
[%]
[%]
[µg/L]
[µg/L]
[µg/L]
Fe
Total
[%]
[µg/L]
1
1.27
22.7
240
6.7 5500 6.39
4.9
100.0
146
71.8
2
1.48
18.1
7
3.3 4900 0.07
0.1
69.9
3
55.3
3
0.85
11.1
6
1.0 5100 0.04
1.4
1.5
1
42.5
4
0.84
14.3
6
1.6 5200 0.05
0.1
52.2
1
58.5
5
0.58
17.5
4
7.7 5500 0.09
0.2
71.4
1
100.4
1
35.52
17.0
140
4.0 5000 0.24
0.9
49.7
113
53.6
2
11.51
42.8
106
5.0 5000 0.18
0.9
44.0
102
53.8
3
12.72
95.9
330
4.5 4900 0.80
2.2
90.3
380
67.6
4
14.63
27.0
200
3.7 5400 1.01
1.3
48.7
143
47.4
5
11.16
48.0
240
4.2 5600 0.85
1.9
44.0
200
46.7
6
25.30
16.7
270
3.8 5900 0.39
4.2
27.4
210
44.9
7
11.63
35.3
210
4.2 5200 0.70
1.9
23.1
140
51.1
8
2.21
100.0
139
4.6 5500 0.41
1.3
63.8
85
75.9
1
0.72
9.8
8
0.3 5900 0.03
0.3
2.1
1
12.6
2
0.72
7.6
5
0.3 6700 0.03
0.1
7.1
1
4.0
3
0.68
6.0
6
0.8 6800 0.03
0.4
3.6
1
25.3
4
1.36
4.6
12
0.9 6500 0.03
0.1
45.6
3
22.7
5
1.22
5.4
10
1.6 5900 0.04
0.1
72.0
3
68.7
1
13.16
18.4
460
0.4 1380 0.40 350.0
0.1
960
17.7
2
22.94
30.5
590
1.1
0.2
2100
28.6
3
5.71
47.0
68
4.1 2200 0.45
49.0
1.0
490
52.8
4
9.62
21.9
200
2.1 2200 0.53 190.0
0.5
480
43.7
5
13.97
45.2
117
6.9 1700 1.37
2.8
100.0
630
62.8
6
1.46
7.8
200
0.1 2800 0.00
92.0
0.0
21
7.8
7
5.55
25.3
200
1.7 2600 0.76 120.0
0.7
250
39.3
1
6.03
19.0
21
0.4
0.12
0.2
100.0
13
36.7
2
8.30
20.0
24
0.4 1640 0.28
0.4
57.8
61
16.3
3
5.76
18.3
21
0.4
0.01
0.1
99.2
15
42.3
4
5.31
17.6
16
0.4 1010 0.11
0.1
100.0
10
59.2
830
700
900
3
1.83 240.0
Figure S1: Exemplary UV signal (primary y-axis) and hydrodynamic diameter (measured by dynamic light
scattering (DLS), secondary y-axis) showing the three fractions, the peak elution times of standard reference
material (green lines, HS = Suwanee River Humic Acid II, FS = Suwanee River Fulvic Acid II, 21 nm/57 nm/60 nm
= Latex standard in the respective sizes) and the separation of the void peak (VP). The DLS signal was used to
determine the size of the 3rd fraction.
1st
3rd fraction
2nd
VP
HS/FS
4
21nm
57nm
60nm
Figure S2: Exemplary raw data of UV absorption at 254 nm for stream source (stream point 1), catchment
outlet point (largest stream point number per site, e.g. stream point 5) and an intermediate point (e.g. stream
point 3) at each of the five sampling sites. Y-axes show UV absorbance in volts (V), x-axes show method runtime
in minutes (min). At Conventwald (CON), the remaining stream points show a fractionation pattern like stream
point 3; at Mitterfels (MIT) stream points 2 and 4 to 7 show a similar pattern to stream point 1; at Vessertal
(VES) stream point 2 shows a similar pattern to stream point 1 and stream points 4 to 5; at Wüstebach (WU)
stream point 2 shows a similar pattern to stream point 1, stream points 3 and 5 to stream point 4 and stream
point 6 to stream point 7; at Leirelva (LEI) stream point 3 was like stream point 4.
5