Thermo Scientific TSQ 8000 Evo
Triple Quadrupole GC-MS/MS
Expect more productivity
with the evolution of ultimate performance GC-MS/MS
Expect
More
Productivity
Evolving with the changing laboratory
environment
The pressure on analytical laboratories has never been greater. Managers face a dynamic
environment of changing regulatory requirements, lower detection levels, more compounds,
less time, lower budgets and increased competition. Laboratories must evolve to stay
ahead of the game, and their analytical systems need to evolve, too.
The Thermo Scientific™ TSQ™ 8000 Evo Triple Quadrupole GC-MS/MS is for labs
looking for the next step up in triple quadrupole GC-MS/MS productivity. It is the latest evolution of the
highly successful TSQ 8000 GC-MS/MS system and the evolution of unstoppable productivity, MS/MS
simplicity, and ultimate performance SRM.
The TSQ 8000 Evo triple quadrupole GC-MS/MS has been designed for and developed with high
throughput analytical laboratories. It is a unique system that offers a harmonious combination
of hardware and software features that help laboratories adapt to their changing
environment and deliver quality results on time, every time.
2
3
Expect
More
Capacity
Fast EvoCell technology and intelligent
instrument control
More capacity is offered through the acquisition of more information, more compounds, and more results per unit time in your laboratory.
This enables higher levels of productivity and efficiency in analytical workflows. Innovative EvoCell collision cell technology, in combination
with the high efficiency timed-SRM capability of the TSQ 8000 Evo GC-MS/MS, opens the door to new possibilities for high-complexity
methods in routine laboratories.
ƒ
Enhanced velocity optics
driving EvoCell collision
cell technology provide
high SRM transition
speeds, precision, and
sensitivity for even the
most complex methods
8
3
TSQ 8000 Evo GC-MS/MS Timed SRM
• Complicated set-up
• Automated set-up
0
• Wasted dwell time
• Optimized dwell time
• Reduced sensitivity
• Maximized sensitivity
• Reduced tolerance to RT shifts
• Increased resistance to RT shifts
lpe
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He
xa
Fo
dio
ha
op
Cy
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900
pr
800
lor
300 400 500 600 700
Transition Speed (MRM/Second)
Ch
200
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Traditional Segmented SRM
1
nil
2
m
EvoCell Technology
Older Cell Technology
100
Multiple single quadrupole pesticides methods consolidated into one 40-minute, 350 pesticides (700 transitions) run
4
50
0

5
Flu
100
Simply enter the retention time and the time
required to capture the peak, and t-SRM takes
care of the rest. This ensures that compound
detection is optimized for maximum sensitivity,
and more compounds can be added to the
method without compromising the excellent
sensitivity of each individual analyte. The
mass spectrometer is not consuming valuable
resources scanning for compounds at the
times when those compounds are not eluting.
6
ini
l
DD
E,p
150
As more compounds are added to a single
run, managing acquisition windows becomes
increasingly complex. The TSQ 8000 Evo GC-MS/
MS system dramatically reduces this complexity
by automatically optimizing the targeting of a
particular compound.
7
rod
200
3.5x sensitivity at faster
acquisition speeds
Bifenthrin Detection Limit
(fg On Colum)
Similar sensitivity at 4x transition speed
0
%RSD
MDL (pg/ul)
Calibration linearity (r2) 5-500ppb
9
250
Truly powerful methods use speed efficiently

Increased sensitivity at fast transition speeds allows the use of up to
4x transition speeds of standard collision cell technology

Analysis of pesticides in rice (10 µg/kg) with the EvoCell collision cell
at 500 µs dwell time, acquiring 800 transitions per second
EvoCell Collision Cell Technology
Features
Benefits
Impact
Increased number of
transitions per compound
More points of confirmation
More resistance to matrix interference
Higher confidence
More on-time results
Increased number of compounds
Higher capacity methods
More method consolidation
More efficiency in result production
Fast GC compatible
Faster run times
Faster turnaround
More on-time results
Wider SRM windows
4
More resistance to the effects of RT
shift caused by matrix
Faster turnaround
More on-time results

Traditional Segmented SRM

TSQ 8000 Evo GC-MS/MS Timed SRM
5
Expect
More
Performance
Ultimate sensitivity SRM
Ultimate sensitivity for contaminants regulated
at the lowest levels
Laboratories performing analyses in food safety, environmental monitoring, and sports doping can all benefit from high selectivity and ultimate
sensitivity selected reaction monitoring (SRM) enabled by the TSQ 8000 Evo GC-MS/MS, which is the highest performing triple quadrupole
GC-MS/MS offered on the market.
The confirmatory determination of polychlorinated
dibenzodioxins and furans (PCDD/Fs) is one of the most
challenging of the ultra-trace analytical methods in
contaminant analysis. This challenge is due to the
extremely low regulated levels and stringent analytical
criteria in force. Meeting that challenge requires
extremely powerful SRM that the TSQ 8000 Evo
GC-MS/MS delivers routinely.
TSQ 8000 Evo pesticide detection limits (1µL injection)
1
0.9
„
0.8
2,3,7,8-TCDD
319.89 > 256.93
ƒ
Fish meal containing low
levels of 2,3,7,8-TCDD
2,3,7,8-TCDD
321.89 > 258.93
13C 2,3,7,8-TCDD
331.94 > 267.97
0.7
parts-per-billion
Detection limit (99% confidence level)
of 166 pesticides based upon 0.5
and 1 ppb peak area precision
(no internal standard correction)
using 1 µL PTV injection (n=10)
Fish meal 0.03 ng/kg 88% dw
0.6
13C 2,3,7,8-TCDD
333.93 > 269.97
0.5
0.4
0.3
0.2
Triallate
Triticonazole
Tolclofos-methyl
Quintozene
Tebufenpyrad
Tetraconazole
Propham
Pyrifenox-Z
Pirimicarb
Oxadiazon
Phosalone
Pentachloroaniline
Napropamide
Linuron
Mevinphos
Isocarbamid
Methoxychlor
Furalaxyl
Hexazinone
Flusilazole
Fenvalerate
Flucythrinate
Fenarimol
Fenhexamid
Etofenprox
Etaconazole
Diniconazole
Endosulfan ssulfate
Difenoconazole
DDE o,p
Dichlobenil
Deltamethrin
Cyfluthrin
Cypermethrin
Chlorothalonil
Chlorthal-monomethyl
Cadusafos
Chlorfenson
Atrazine
ƒ
0.07
Instrument detection limit (IDL) for PCDD/Fs
when satisfying all confirmatory criteria CSL x 5
dilution (n=10) injected using splitless mode
0.06
0.05
0.04
0.03
0.02
DDE p,p’ 5 µg/kg
in green tea
0.01
Oxyfluorfen
Calibration Curve
5 - 500 µg/kg in
green tea

High selectivity of low concentrations in complex tea matrix
23
DDE p,p’ Calibration
Curve 5 - 500 µg/kg
in green tea
78
-T
CD
23
78 F
12 -TCD
37
D
8Pe
23
CD
47
F
812 PeC
D
37
8- F
Pe
12
34 CDD
78
12 -Hx
C
36
78 DF
23 -Hx
C
46
78 DF
12 -HxC
34
78 DF
12 -Hx
C
36
78 DD
12 -HxC
37
DD
89
H
12
x
37 CDD
8
12 9-H
x
34
67 CDF
12 8-H
p
34
67 CDF
12 8-Hp
34
CD
78
D
9Hp
CD
F
OC
DD
OC
DF
0
Oxyfluorfen 5 µg/kg
in green tea
6
0.08
Concentration [pg/µL]
The TSQ 8000 Evo GC-MS/MS
is a perfect fit for laboratories in
need of high efficiency pesticide
methods. Enhanced selectivity,
speed, and sensitivity translate
into real routine efficiency and
productivity. Expect performance
above and beyond former triple
quadrupole instruments.
Bifenthrin
0
BHC, Alpha
0.1
0.09
2,3,5,6-Tetrachloroaniline
0.1
European Commission GC-MS/MS
Confirmatory Performance Criteria
TSQ 8000 Evo GC-MS/MS
Capabilities
Compliance
Confirmed
Two specific precursor ions with two specific
production ions
All recommended methods developed
as defined in criteria
Fully optimized by AutoSRM
Yes
Tolerance of ion ratios within ± 15%
< 10% measured at EPA 1613 CSL level (n=14)
Yes
Resolution of each quadrupole equal to or better
than unit mass resolution
All recommended methods developed
Q1 and Q3 at 0.7 Da
Yes
The % RSD of the five (or more) Relative
Response Factors (RRFs) for each unlabeled
PCDD/PCDF and labelled internal standards
must not exceed 20%
6 point curve EPA 1613 CSL-CS4
2%
Yes
7
Expect
More
Expect
Robustness
Flexibility
Unstoppable productivity
Flexibility to best solve your analytical challenge
The Thermo Scientific™ ISQ™ Series single quadrupole GC-MS and TSQ 8000 triple quadrupole GC-MS/MS have a strong reputation in allowing
laboratories to tackle the demands of producing results on time, every time. The TSQ 8000 Evo triple quadrupole GC-MS/MS continues that
legacy by enabling routine-grade robustness for ultimate performance applications in some of the most challenging matrices.
The TSQ 8000 Evo GC-MS/MS addresses many laboratory mass spectrometry needs through various scan modes along with source and probe options.
Excellent performance is enabled for single quadrupole-type scan modes which, in turn, expand system versatility in the modern laboratory.
100
Biphenyl (3.6% RSD)
Ortho-phenylphenol (3.7% RSD)
Butylated hydroxyanisole (4.2% RSD)
Phorate (3.1% RSD)
Tetrahydrophthalimide (4.7% RSD)
75
25
0
1
6 11 16 21 26 31 36 41 46 51 56 66 61 71 76 81 86 91 96
Injection #
Robustness of 1:20 diluted orange oil spiked with pesticides and injected
directly (n=100)
14
12
10
10
8
8
6
% RSD
12
Q2 (EvoCell)
Q3
2
2
0
0
Application
Selected ion monitoring (SIM)
Transmission only
Transmission only
Selected m/z
Target quantitation
Full scan
Transmission only
Transmission only
Scanning
Unknown ID, library search
Selected reaction monitoring (SRM)
Selected m/z
CID
Selected m/z
Target quantitation
Product ion scan
Selected m/z
CID
Scanning
SRM method development and
compound elucidation, build and
search libraries
Precursor ion scan
Scanning
CID
Selected m/z
Searching for unknown compounds
giving characteristic fragments
Constant neutral loss
Scanning
CID
Scanning (- neutral loss offset)
Searching for unknown compounds
with common structures, e.g. acids
SRM/Full Scan
Alternating selecting m/z and
transmission only
Alternating CID/ Transmission only
Alternating selecting m/z and
scanning
Target quantitation and unknown ID
by library search
Ion sources and probes are removable without requiring a break in the vacuum, and they enable you to perform
MS and MS/MS experiments on solids in real time while effortlessly switching between electron ionization (EI)
and chemical ionization (CI).
Direct Insertion Probe (DIP)
Slower volatilization with heated capillary
tube for solid samples. The DIP can be
utilized for rapid analysis of solids or trace
components in solid matrices such as
forensic samples, tissue, etc.
Direct Exposure Probe (DEP)
Rapid heating filament for liquids or solutions.
The DEP is ideal for rapid molecular weight
confirmation of solids dissolved or suspended
in a suitable solvent.
Chemical Ionization
Chemical ionization is a softer form of
ionization than electron ionization (EI).
It offers a greater opportunity to generate
more abundant high mass and molecular
ions and a higher degree of selectivity or
sensitivity in the source ionization process,
especially with negative chemical ionization
(NCI), favoring electronegative compounds,
such as halogenated species. Chemical
ionization is ideally used for structure
elucidation also in MS/MS mode.
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Ipr n
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ion
Az
inp
ho EPN
sMe
Pe thyl
m
De ethr
lta in
me
thr
in
4
%RSD pesticides (10 ppb) before and after 1,400 vegetable matrix injections
After
6
4
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Tr hob
ibr
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om nil
oa
nis
o
Su l
lfo
tep
H
Pa CB
rat
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Ipr n
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ion
Az
inp
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sMe
Pe thyl
m
De ethr
lta in
me
thr
in
% RSD
Before
Di

Q1
50

14
Mode
Robustness Cold Pressed Orange Oil (external standard calibration)
pg/µL
The patented Thermo Scientific™
ExtractaBrite™ ion source is heated throughout
and includes the first RF region (RF lens),
commonly the first place susceptible to ion
burn. This design ensures high
matrix tolerance for high
throughput applications. Crucially, it is
fully removable, even when hot, under
vacuum for necessary cleaning or
swapping with a spare source. There
is never a need to vent to clean
your instrument or to heat or
clean quadrupoles. Enjoy
constant productivity.
8
More
9
brilliant
Design
for brilliant
Industry-Leading Detector Linearity
Results
The Thermo Scientific DynaMax detection system standard on the TSQ 8000 Evo system offers industry-best linearity.
Combined with the low detection limits attainable by SRM, this detector makes the mass spectrometer
the ultimate quantitative instrument.
EvoCell Collision Cell Technology
High performance SRM at high velocity
transition speeds.
Remove the Source of your Downtime
Allows full source removal without venting
the MS including ion guide surface. No wire
connections are necessary, and venting is
never necessary to clean your instrument.
Never Clean or Replace Quads
Heated ion guide protects the
main quadrupole sets so
they never require cleaning
or replacement.
ExtractaBrite wireless
ion source cartridge
Switch Between Full Scan,
SIM and MRM in Milliseconds
Effortless switching is made
possible with the fast scanning
capabilities of the TSQ 8000 Evo
GC-MS/MS.
Dual Heated Zones on Source Maximize Ruggedness
Dual independently-heated zones on the TSQ 8000 Evo
system allow for thousands of injections of the dirtiest
matrices before source cleaning is required.
No Helium Required
Choose between nitrogen
or argon for collision gas.
No expensive helium
is required.
Fast Scanning Allows Fast Chromatography
Dynamic ion expulsion in the collision cell allows
hundreds of MRM transitions per second.
Combined with t-SRM for efficiently managed
transition scheduling, the TSQ 8000 Evo system
can analyze hundreds of compounds with multiple
transitions each over short chromatographic runs.
On-Axis (in-line) Optics
10
Dual Filaments for Prolonged Uptime
Intelligent dual filament design keeps
you running longer between
maintenance intervals.
No Noise is Good Noise
Off-axis optics provided by the curved ion
guide effectively eliminate neutral noise,
resulting in less background and lower
limits of detection.
noise reduced
to ~ 2 ions/sec
11
Expect
More
Simplicity
AutoSRM Workflow
Easy MS/MS
Your Starting Point
The advantages of selected reaction monitoring (SRM) and multiple reaction monitoring
(MRM) analysis often come with challenges, especially with respect to adoption of
new technology in the laboratory. These challenges are due to the method set-up and
optimization and management of complex analytical methods. Laboratories that strive
to realize the benefits of MS/MS must overcome some barriers in the tuning, set-up
and optimization to reduce the time required to achieve a smooth operating routine.
• AutoSRM: A purpose-built software for automated SRM method creation
and optimization from full scan to the complete analytical method setup
• TSQ 8000 Evo GC-MS/MS Instrument Method: Offers true
timed-SRM operation, allowing for high sensitivity and ease of use for the
most complex SRM methods
Unknown SRM transitions
• Start from the very beginning
• Start with existing SIM method
AutoSRM
SRM Optimization
The TSQ 8000 Evo GC-MS/MS was built to guarantee an easy start-up. Whether
you are managing retention times, starting a completely new analysis, transferring
a method from a single quadrupole system, or porting a known MRM method from
another instrument, the TSQ 8000 Evo system ensures the fastest route to routine
high performance results through its integrated software tools.
From a Previous MS/MS Method
If you have already performed previous AutoSRM steps or are simply moving from another
MS/MS system, then Step 3 can take you to the final fully optimized SRM transitions for
your compounds. Fully automated collision energy optimization is performed to ensure
that transitions are as sensitive as they can be. These optimized SRM transitions are then
exported into a finished MRM method ready for analyzing real samples in routine.
12
TSQ 8000 Evo
Instrument
Method
• From an existing method
• From SRM transition list
Method Sync
From an Existing SIM Method
If you are moving from a single quadrupole GC-MS method using SIM analysis, or you
already know which ions you want to select for your precursor ions, you can start with
Step 2. The AutoSRM function performs product ion scans for each selected precursor
and presents the results in a single window. Simply select (or ask AutoSRM to select) the
best product ion for that compound.
Step 2: Selects your product ions from product ion scans
Known SRM transitions
• Thermo Scientific™ TraceFinder™ Software: State of the art,
multi-platform user-friendly chromatography analysis software
From the Start
For new compounds, choose to begin the AutoSRM function, ask for a precursor ion study
(Step 1), and place a vial with the compound mix in the autosampler. The AutoSRM function
reports a full scan chromatogram of the injection, and you simply identify your peaks and
choose the most appropriate precursors that AutoSRM presents. In the case of coeluting
compounds, AutoSRM automatically performs one injection per compound.
Step 1: S elects your precursor ions from full scan
Step 3: O
ptimizes the collision energy for selected
transitions
Optimized SRM transitions
• From Compound Data Store
• From existing GC-MS/MS method
TraceFinder
Software
13
Be
More
Productive
Fast, flexible, easy-to-use
mass spectrometry software
The challenge
The constant pressure to increase productivity challenges laboratory
staff at every level, from the technician to management. Streamlined,
workflow-driven software solutions are needed to address these
challenges. At the same time, the growing list of compounds that
laboratories must quantify and screen, combined with increasingly
complex regulatory requirements, necessitate flexible method
development and powerful reporting tools.
Powerful method development
TraceFinder software provides powerful tools for method
developers, including:
TraceFinder software is the solution
TraceFinder software makes routine quantitation simple, fast
and productive. It offers a complete solution that can be used
to develop methods, acquire and process data, and generate
standard and customized reports with the full portfolio of
Thermo Scientific mass spectrometers.
• Automated retention time and ion ratio adjustment
•C
ompound databases (CDB) for rapid selected
reaction monitoring (SRM) method development
• User-defined flags for nearly every parameter
Leading the way in regulatory POPs quantitation
Thermo Scientific TargetQuan software is uniquely tailored for a
workflow-orientated POPs quantitation, like PCDD/Fs, PCBs,
PBDEs and others. This software package is designed specifically
to serve laboratories charged with performing routine quantitation
of POPs in a regulated environment. With TargetQuan 3 software,
comprehensive processing of MS, MS/MS and HRMS data is
possible on a single software platform, compliant with the
US EPA 1613 requirements.

Compound Database contains hundreds of compounds and parameters for SRM work flows, allowing rapid method development
14
Simple, full-featured data review
TraceFinder software offers simple, yet exceptionally feature-rich
data review capabilities. The intuitive, workflow-driven arrangement
of information simplifies the technician’s role in reviewing data.
The technician can quickly and easily observe the data processing
status, view compound-related flags to locate compounds which
need attention, and take appropriate steps to address any problems.
Powerful, automated reporting
The new TraceFinder software report designer offers enhanced
laboratory productivity with customizable, automated reporting
with numerous standard report templates.
15
More
Experience
innovation
„
Example of plug-in module
installation by user
Tackle future challenges through
GC modularity
Enable Quick Customization with GC Modularity
Tailor the Thermo Scientific™ TRACE™ 1300 Series GC to your needs with its proprietary user-exchangeable Instant Connect
injector and detector modules. Swapping modules is easily done by the removal and replacement of three screws, accessible
from the top of the GC. The entire process takes less than five minutes without requiring specialized service assistance. This
enables budget-conscious laboratories to purchase a base-configured GC today, then easily expand their capabilities
to accommodate new application and throughput demands.
Ensure Uninterrupted Analyses and Save Budget
by Conserving Helium
The helium shortage continues to inflict price pressure and supply
uncertainty on laboratories that require these gas supplies to
perform their research and analyses.
Estimated Cylinder Lifetime
GC and GC-MS
Operating Conditions*
Conventional Operation
With the Instant Connect
Helium Saver Module
Around the Clock Analysis
24 hours a day, 7 days a week,
365 days a year
5 months
3.5 years
Daily Operations
Leaving the GC idle at the end
of workdays and on weekends
15 months
Realizing the importance of finding a safe, cost-effective solution, the
proprietary Instant Connect Helium Saver Module has been introduced
to drastically reduce helium carrier gas consumption and extend
helium cylinder lifetime up to 14 years per instrument, without any
GC or GC-MS method modifications. Helium is continuously saved,
both while the GC is in operation and while it is idle. Previously
acquired retention times remain unchanged, and no method
revalidation is required.
14.6 years
* Conditions: Operating a TRACE 1300 Series GC with helium at 4 mL/min (sccm) using a typical
helium cylinder of 48 L volume at 2250 psig.
RT: 2.01 - 13.60
10.32
100
90
11.25
6.86
80
70
4.58
60
50
3.51
40
3.56
3.26
3.85
4.69
5.20 5.30
4.28
5.05
3.95
30
5.36
5.47
6.11
9.03
7.88 7.93
6.29
5.68
12.71
7.00
6.48
7.40
7.47
7.04
7.67
5.97
8.45
8.09
9.81
9.88
10.86
13.05
11.56
20
2.58
7.12
10
2.52
0
100
4.97
2.78 3.22
8.18
8.53
6.81
10.77
9.62
12.01 12.18
80
8.98
4.55
70
60
50
3.48
3.54
4.49
3.66
4.25
3.82
40
3.37
30
5.63
4.66
6.06
6.44
6.95
5.32
5.01
5.46
5.93
8.05
12.63
11.45
12.95
8.41
7.62
9.77
9.84
9.33
10.81
11.50
„
10.72
3.0
8.11
4.94
2.77 3.19
2.5
11.17
Backflush and Large Volume Capabilities
The capabilities of the Instant Connect injector modules are further
enhanced by the available concurrent backflush options. These
solutions enable the user to reverse the flow inside the injector,
eliminating heavy or “undesired” compounds concurrently during
the analysis run, protecting the column and detector while cutting
down non-productive times, thus increasing throughput.
7.08
2.57
2.15
9.19
7.00
20
10
7.42
7.35
6.57
4.15
3.24
11.19
7.84 7.89
6.25
5.16 5.26
13.19
10.28
10.25
90
0
11.52
11.22
9.23
3.5
4.0
4.5
5.0
5.5
6.0
6.5
7.0
7.5
8.0
Time (min)
8.48
8.5
10.39
9.58
9.0
9.5
10.0
10.5
11.94 12.10
11.0
11.5
12.0
12.5
13.0

Instant Connect-SSL concurrent
backflush. The T-piece for column
connections and its carrier gas control
are integrated within the module
Using the Instant Connect Helium Saver Module, results from US EPA Method 8270C
(semi-volatiles) remain unchanged
www.thermoscientific.com/tsq8000evo
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