LUCIA Cytogenetics
Systems for Karyotyping and Molecular Cytogenetics
LUCIA KARYO
Karyotyping system
LUCIA KARYO is a specialized product
for automated karyotyping. In addition
to offering the basic functions, it also
offers: grabbing of widespread mitoses,
a special band contrasting procedure,
and an automated chromosome classifier. These are just a few examples of
the power LUCIA has in karyotyping.
The LUCIA KARYO system provides
many great features:
• Structured database for archived
storage of patient data and images.
• Integrated flexible reporting tool.
• Analysis of arbitrary animal or plant
species.
• Karyogram templates, ideograms,
report templates and sample database.
• Provides a standard for working with
all LUCIA Cytogenetics products.
The LUCIA karyotyping system provides an
easy-to-use tool for chromosome comparisons using an image database. Up to three
types of chromosomes can be compared
with an unlimited number of source images.
An image of a metaphase (upper picture). Result of the semiautomated chromosome classification of a female patient with
normal karyotype 46, XX (lower picture)
Author: G. Kistner,
Zytogenetisches Labor Schwerin, Germany.
LUCIA FISH
Fluorescence in situ hybridization system
LUCIA FISH offers a uniquely designed workstation used for fluorescence microscopy in
general. Its extraordinary flexibility allows capture and processing images of extremely variable intensities. A high sensitivity long time exposure monochrome camera provides superb
image quality and minimal signal fading. A
camera exposure control enables efficient
capturing, that can be enhanced by motorized
microscope usage. The user gets a multichannel image in just a few seconds. The
number of labeling probes is now limited only
by human abilities (the number of image channels are restricted to 16 for convenience).
LUCIA FISH provides a variety of automated
and manual image corrections including filtershift correction or color enhancement. The
preprint transformation defines the different
Pic 1.
basic color for each channel for
printing usage and offers the
possibility to optimize quality of
printouts.
The LUCIA FISH system defines a way to work with multicolor fluorescence images for all
LUCIA Cytogenetics systems and
shares the database and reporting tools with other members of
the LUCIA Cytogenetics family.
The specimens labelled with
Vysis MultiVysionTM PGT kit..
See the bellow table for the
labelling details.
Pic 2.
(Author: O. Scheinost, Dept. of
Medical Genetics, Hospital
České Budějovice, Czech Rep.)
Target
Labelling probe
Color channel – pic. 1
Color channel – pic. 2
13
SpectrumRedTM
red
red
cyan
cyan
green
green
TM
18
SpectrumAqua
21
SpectrumGreenTM
TM
X
SpectrumBlue
magenta
blue
Y
SpectrumGoldTM
yellow
yellow
Counterstain
DAPI
blue
magenta
LUCIA CGH
Comparative genomic hybridization system
Pic 1.
Pic 2.
Pic 3.
LUCIA CGH is an application able
to detect and map the relative
number of copies of the DNA sequence using a method called
Comparative Genomic Hybridization (CGH). Image analysis and
mathematical statistics are used
to determine the loss or amplification of DNA sequences. The
module for color karyotyping and
graphic presentation of DNA profiles is an integral part of the system. One image plane corresponds to the control DNA, the
second to the tested DNA, and
the third, usually the blue, represents the counterstaining with
DAPI.
LUCIA CGH provides full karyotyping functionality combined
with fluorescence imaging features. The images can be observed in various display modes
in each step of karyotyping procedure. Also enabled is the evaluation of the individual CGH profile
for each chromosome.
The database tools simplify
analysis to a few mouse-clicks.
CGH analysis results are displayed in the form of graphs with
a variety of customizations
(scales, thresholds, confidence
intervals etc.)
Pic. 1 and 2: Co-hybridisation of the
marked control and studied DNA in
normal metaphase chromosomes.
Metaphase spread acquired in the
three fluorescence channels and
respective karyogram.
Staining: red– SpectrumRedTM,
green –SpectrumGreenTM,
Counterstain – DAPI.
(Author: J. Sobotka, Dept. of Medical
Genetics, Children University Hospital
Brno, Czech Rep.)
Pic. 3: Computer processed ratio of
the signal intensities of both fluorochromes measured on the result chromosomes. The shifts to the red and
green area represent deletions and
amplifications respectively or vice
versa.
LUCIA MFISH
Multicolor fluorescence in situ hybridization system
Pic 1.
LUCIA MFISH is the fluorescence imaging system extending the features
of KARYO and FISH systems. It enables the full variety of the karyotyping features in the multi-color fluorescence environment. In addition, it
provides the color classifier suitable
for analysis of standard multi-color
FISH images.
The color classifier needs only a priory information in the form of the labeling scheme and is suitable for all
combinatorial labeling schemes.
LUCIA MFISH offers all common features of LUCIA Cytogenetics system
such as database, report tools, etc.
Pic. 1: Specimen of two years old girl
with diagnosis of AML (acute myeloid
leukemia), bone marrow material.
The metaphase spread captured in six
color channels and displayed in quasi-real
colors.
Pic. 2: The metaphase with segmented
chromosomes. Displayed in pseudo-colors.
Pic. 3: Karyogram in pseudo-colors. The
examination have proved the nonreciprocal translocation of the part of the
chromosome 6 to the chromosome 16.
(Author: P. Kuglík, Faculty of Science,
Masaryk University Brno, Czech Rep.)
Pic 2.
Pic 3.
Laboratory Imaging cooperates with
many research and routine laboratories. LUCIA Cytogenetics reacts to
the most recent demands and practice. We offer full support to the customers including e-mail and phone
consulting and training by our coworking labs.
Laboratory Imaging s.r.o, Za Drahou 171/17, CZ-102 00 Praha 10 - Hostivař
Tel: +420-272 081 400, e-mail: [email protected], web: www.lucia-cytogenetics.com