Unit conversation
1 mmol/l = 0.2816 mg/dl
mmol/24 hod = 0.0282 mg/24 hod
CHLORIDE Liquid 250 Cat. No. 10003276 (Cl L 1 x 250)
Store at (+2 to +25) °C
Liquid reagent for photometric determination of chloride in biological
material.
Principle
Chlorides ions release from the reagent with thiocyanate ions which
react with Fe3+ and form red ferric thiocyanate. The intensity of the
colour is proportional to the chloride concentration in the sample.
2 Cl– + Hg(SCN)2
SCN– + Fe3+
HgCl2 + 2 SCN–
Fe(SCN)2+
Reagents
R1 Reagent Mercury (II) thiocyanate Iron (III) nitrate Nitric Acid R2 Standard Chloride 1x250 ml
2 mmol/l
20 mmol/l
29 mmol/l
1x5 ml
100 mmol/l
Reaction mixture
Mercury (II) thiocyanate Iron (III) nitrate Nitric Acid 1.98 mmol/l
19.8 mmol/l
28.7 mmol/l
Preparation and stability of working reagents
Reagents R1 and R2 are liquid, ready to use.
If stored at (+2 to +25) °C, reagents are stable until expiry date, that
is stated on the package.
After opening, reagents are stable until expiry date at (+2 to +25) °C
if stored at appropriate conditions, in the dark place, closed carefully
and without any contamination.
Samples
Serum or plasma (heparin), urine.
Stability of chloride in the serum: 2 weeks at (+4 to +8) °C.
Calibration
For the calibration, it is recommended to use Lyonorm Calibrator,
Cat. No. 10003200 or the standard included in the set.
Quality control
For quality control, it is recommended to use:
Lyonorm HUM N, Cat. No. 10003204
Lyonorm HUM P, Cat. No.10003206
Procedure
Wavelength Cuvette Temperature Serum/reaction mixture ratio 480 nm
1 cm
+37 °C
1/101
Reagents and sample volume can be modified, by respecting reagents/sample volume ratio
Reagent
Standard
Sample
blank
(Calibrator)
Reagent 1
1.00 ml
1.00 ml
1.00 ml
Sample
–
–
0.01 ml
Standard
–
0.01 ml
–
Distilled water
0.01 ml
–
–
Mix and incubate 5 min. at 37 °C. Measure absorbance of the
sample Asam and standard (calibrator) Acal against reagent blank.
The coloration is stable during one hour.
Applications for automatic analysers will be supplied on request.
Calculation
Asam
Chloride (mmol/l) =
x ccal
Acal
Ccal = standard (calibrator) concentration
Reference values
fS, fP Chloride (mmol/l) 97 – 108
dU Chloride (mmol/24 hours) 110 – 270
The range of reference values is only approximate; it is recommended that each laboratory verify the extent of the reference interval for
their particular examined population.
Performance data
Linearity: up to 110 mmol/l
Limit of detection: 1.4 mmol/l
Low limit of quantification: 4 mmol/l
Working range: 4 – 110 mmol/l
Precision (při 37 °C)
INTRA-ASSAY
n = 20
Sample 1
Sample 2
Sample 3
INTER-ASSAY
n = 10
Sample 1
Sample 2
Sample 3
Mean
(mmol/l)
91.47
98.09
113.20
Mean
(mmol/l)
88.24
99.93
109.32
SD
(mmol/l)
0.69
0.64
1.24
SD
(mmol/l)
2.85
2.91
2.72
CV
(%)
0.76
0.65
0.88
CV
(%)
3.23
2.91
2.49
Accuracy:
Bias
– 3.8 % (90 mmol/l)
Correlation: with commercial available method. Linear regression
n = 51, r = 0.97, y = 0.94 x + 5.52 mmol/l
Interference
Following substances do not interfere:
Ascorbic acid up to 0.6 mmol/l, bilirubin up to 500 µmol/l, triglycerides
up to 7 mmol/l, hemoglobin up to 5 g/l.
Notes
1. For the determination in urine use the specimen collected during
24 hours period. To gauge urine exactly. If the chloride concentration exceeds 110 mmol/l, dilute the sample by distilled water in
ration 1 + 1 (result 2x).
2. Glassware use for chloride determination should be perfectly
clean and should not be used for other purposes. After a superficial washing by the ordinary way it is recommended to dip the
glassware overnight in chlorosulfuric acid, then to rinse thoroughly
using water and finally redistilled water and dry.
When working on automatic analyser, it is recommended to clean
often cuvettes by Extran.
Health protection
For in vitro diagnostic use.
To be handled by entitled and professionally educated person.
Reagents of the kit are not classified like dangerous.
First aid
In case of an accidental ingestion, wash up the mouth and drink
about 0.5 l of water. On eye contact rinse the eye quickly and thoroughly with the jet of tap of water. Contaminated skin should be
washed with warm water and soap. In all serious cases of health
damage consult a physician.
Waste disposal
All tested samples should be treated as potentially infectious and
with an eventual rest of reagents should be disposed in accordance
with the internal regulations for dangerous waste, in compliance
with local and national regulations relating to the safe handling of
dangerous materials.
Paper packing and others should be handed over for recycling or
discarded as sorted waste (paper, glass, plastic).
References
1. Schoenfeld, R. G., at al.: Clin. Chem., 10, p. 533, 1964.
2. Zima, T.: Laboratorní diagnostika, Galén (2002)
Date of last revision: 7. 10. 2009