Botany and Medicinal Plants (201) First year Students Seventh Practical Session Pharmacognosy Department 2016/2017 Digitalis Leaf 11/12/2016 2 Digitalis leaves Digitalis purpurea Digitalis lanata (Due to the purple color of the flower) The official drug 11/12/2016 non-official drug 3 Digitalis lanata I-Morphology • Name : Digitalis Leaf. • Origin: The dried leaves of Digitalis lanata, family Scrophulariaceae, collected and rapidly dried at 55-60°C. Why?!! The main active constituents are Cardiac glycosides which are sensitive to: high temperature and moisture , so it should be rapidly and at temperature not exceed 55-60 ̊C to avoid breakdown of the glycosides and loss of activity. • Colour : Green. • Condition: dried, broken pieces and moistened. • Insertion: ?? 11/12/2016 Radical Insertion 4 I-Morphology Acute • Leaf petiole: apex 1. Occurrence: sessile Broadly 2. Characters: ------dentate 3. Pulvinous : ------margin Lamina: 1. Composition :simple. 2. Incision: ------3. Shape : oblanceolate, linear, lanceolate. Symmetric base 4. Apex : acute. 5. Margin : broadly dentate margin. 6. Base : symmetric. 11/12/2016 Entire ,ciliate at the base. 5 7. Venation: pinnate reticulate venation, veins leave midrib at narrow acute angle. (appears as parallel venation) 8. Size: as shown (length in cm. x width in cm.) 9. Surface: • upper: green, glabrous to the naked eye. • lower: pale green, glabrous to the midrib naked eye. -midrib is prominent to the lower surface Pinnate reticulate and depressed on the upper. venation • Texture : papery. • Odor : slight tea-like odor. • Taste : persistent bitter taste. 11/12/2016 6 II-Surface Preparation. Upper surface Lower surface Beaded wall Anomocytic stomata 1- Shape 2-Anticlinal walls 3- Cuticle 4- Stomata 11/12/2016 Anisocytic stomata polygonal isodiametric to axially elongated nealry Straight ,beaded smooth Straight to slightly wavy , beaded smooth anomocytic , few anisocytic 7 Upper surface 5-Hairs Lower surface -Glandular hair with unicellular stalk and unicellular or bicellular head. -Glandular hair with multicellular -Non glandular multicellular uniseriate stalk and unicellular unbranched uniseriate hair. head. 6-Content 11/12/2016 ------8 III-T.S. Lamina region Midrib region 11/12/2016 9 Upper epidermis Lamina Region mesophyll Palisade mesophyll Spongy tissue Differentiated (palisade and spongy tissue) undifferentiated (spongy tissue) lower epidermis Type of mesophyll ?!!! Undifferentiated (homogenous) mesophyll 11/12/2016 Chlorenchyma (parenchyma cells containing chloroplast) 10 Midrib Region Vascular bundle Pericycle Cortex 1- Vascular bundle Xylem Vascular bundle phloem One crescent shape and collateral vascular bundle. 11 Midrib Region 2- Pericycle Collenchymatous pericycle 11/12/2016 12 Midrib Region 3- Cortex Parenchyma Lower Collenchyma The cortex is parenchymatous with lower collenchyma. 11/12/2016 13 III-T.S. Undifferentiated Lamina (homogenous) Crescent Collateral Vascular Bundle Collenchymatous Pericycle Parenchymatous Cortex Lower Collenchyma 11/12/2016 14 III-T.S. Description: • Leaf lamina is undifferentiated (homogenous). • it may be differentiated into upper palisade and spongy tissue. • If differentiated, the leaf is dorsiventral. • The palisade (if differentiated )is discontinuous on the upper surface. • The midrib is strongly prominent to the lower surface and depressed on the upper surface. • The midrib region: -Vascular bundle: crescent in shape, collateral in type. -Pericycle: collenchymatous. • The cortex is parenchymatous with lower collenchyma. 11/12/2016 15 IV-Powder of Digitalis Leaf Color: green. Odor: Slight tea-like odour. Taste: Persistant bitter taste. Microscopically characterized by : 1.Fragment of Upper surface Beaded Anticlinal Wall 16 11/12/2016 2.Fragment of Lower surface 3-Fragments of Glandular hair with short multicellular or unicellular stalk and unicellular or bicellular head. 4.Xylem vessels N.B.: No Ca-oxalate No fibers 11/12/2016 17
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