EMB Agar
(Eosin Methylene Blue Agar)
Directions
Suspend 37.5 g of the dehydrated medium in 1,000
mL of distilled water, mix well and heat to dissolve the
medium. Sterilize by autoclaving at 121 °C for 15
The growth of gram-positive organisms is inhibited
by the dyes.
Formula
minutes. Distribute about 20 mL of the medium into
Petri dishes.
Determinations
Incubate at 35 ± 1°C for 22 - 26 hours. E. coli, one of
coliform organisms, forms colonies of metallic luster
with a black center, while Enterobacter aerogenes
forms larger colonies with a dark center. Salmonella
and Shigella form colorless, transparent and small
colonies.
Remarks
The medium is used for the differentiation of
coliform organisms in foods and drinks.
HyServe GmbH & Co.KG, Hechenrainerstr. 24, 82449 Uffing, Germany; www.hyserve.com; [email protected]
produced by Nissui Pharmaceuticls CO., LTD