P r o d u c t
N o t e
Gas Chromatography
Key Benefits
• Reduced tailing for better baseline resolution
• Greater separation of analytes
• Complete resolution of all peaks
Elite-BAC
Advantage
Columns
Introduction
Blood testing is a technique used to determine the Blood Alcohol Content (BAC)
in Driving Under the Influence (DUI) and Driving While Intoxicated (DWI) cases.
In 2009 alone, there were an estimated 1.4 million DUI/DWI cases in the United
States.1 With the volume and severity of DUI/DWI cases, it is imperative to have
high throughput and quality results. That’s why we’ve developed a new line of
Elite-BAC Advantage columns to help you meet those high demands.
Our new Elite-BAC columns eliminate co-elution of key alcohols with internal
standards, and limits peak tailing while keeping run times under 2 minutes.
2
1
RT
Conc.
(min.)(µg/mL)
PEAKS
1. Methanol
0.912
100
0.967
100
2. Acetaldehyde
3. Ethanol
1.158
100
1.420
100
4. Isopropanol
5. Acetone
1.548
100
6. tert-Butanol 1.68850
7. 1-Propanol
1.969
50
RT
Conc.
(min.)(µg/mL)
PEAKS
1. Acetaldehyde
0.809
100
0.861
100
2. Methanol
3. Ethanol
1.034
100
1.119
100
4. Acetone
5. Isopropanol
1.181
100
6. tert-Butanol 1.30450
7. 1-Propanol
1.658
50
4
5
• Clear baseline resolution
4
3
1
5
• Symmetrical peaks
6
3
• Run time under 2 min
6
2
7
7
0.5
0.75
1
1.25
1.5
Figure 1. Elite-BAC 1 Advantage.
1.75
2
0.5
0.75
1
1.25
1.5
Figure 2. Elite-BAC 2 Advantage.
1.75
2
GC_CF
Results
To demonstrate the capabilities of our Elite-BAC Advantage columns we tested them against the Agilent® DB-ALC 1 and
Phenomenex® ZB- ALC 1 columns. In Figure 3, the first chromatogram DB-ALC 1 shows co-elution of acetone and t-butanol.
t-butanol is an internal standard commonly used in BAC analysis which can co-elute with isopropyl alcohol metabolite
acetone under poor chromatography conditions.
Also in Figure 3, the second chromatogram ZB-ALC 1 illustrates tailing of compounds, a common issue in BAC analysis. On the ZB-ALC 1 column, tailing occurs on all compounds when run under the same chromatographic conditions as the
DB-ALC 1 and Elite-BAC Advantage columns.
The final chromatogram in Figure 3, the Elite-BAC 1 Advantage column, exhibits neither the peak tailing nor the co-elution,
with all compounds eluting under 2 minutes.
acetaldehyde
DB-ALC 1
• Co-elution of acetone and t-butanol
• Tailing observed
t-butanol
isopropanol
ethanol
methanol
acetone
n-propanol
0.75
1
1.25
1.5
1.75
2
2.25
ZB-ALC 1
•Tailing on all alcohols and even on
acetone
acetaldehyde
ethanol
•Poor baseline resolution between
methanol and acetaldehyde peaks
acetone
ethanol
isopropanol
methanol
tert-butanol
n-propanol
0.75
1
1.25
1.5
1.75
2
Elite-BAC 1 Advantage
•No co-elution
acetaldehyde
• No tailing
acetone
methanol
ethanol
isopropanol
t-butanol
n-propanol
12
(min.)
Figure 3. Elite-BAC Advantage column results on co-elution and tailings.
2
2.25
Ordering Information
Every Elite-BAC Advantage column is quality tested with target compounds to ensure
performance. These new columns are exceptionally robust, ensuring longer column
lifetime and highly reproducible data. For fast, consistent results, try Elite-BAC Advantage
columns for analysis of blood alcohol content.
Table 1. Elite-BAC Advantage GC Columns
Description
Inner Diameter (mm i.d.)
Length
(m)
Film Thickness
(um)
Part No.
Elite-BAC 1 Advantage
0.32
30
1.80
N9315071
Elite-BAC 1 Advantage
0.53
30
3.00
N9315072
Elite-BAC 1 Advantage
0.18
10
1.00
N9315075
Elite-BAC 2 Advantage
0.32
30
0.60
N9315073
Elite-BAC 2 Advantage
0.53
30
1.00
N9315074
Elite-BAC 2 Advantage
0.18
10
0.34
N9315076
Elite-BAC 3 Advantage
0.18
10
0.30
N9316575
Reference
1.U.S. Department of Justice Federal Bureau of Investigation, 2009. Estimated Number
of Arrests United Sates, 2009. Retrieved from http://www2.fbi.gov/ucr/cius2009/data/
table_29.html
If you’re ready to experience the PerkinElmer Advantage, visit: www.perkinelmer.com/BAC
PerkinElmer, Inc.
940 Winter Street
Waltham, MA 02451 USA
P: (800) 762-4000 or
(+1) 203-925-4602
www.perkinelmer.com
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