HaeIII - 4355 - GG'CC
HaeIII - 4321 - GG'CC
HaeIII - 4310 - GG'CC
HaeIII - 4292 - GG'CC
rbs - 27
tetP
A
TetP
pASK-F/
pASKXho-F
MT
as
e
r ia
nt
HaeIII - 3543 - GG'CC
HaeIII - 3510 - GG'CC
HaeIII - 3469 - GG'CC
HaeIII - 3369 - GG'CC
Tet
pASK HisTag MTase
4461 bp
pASK-R/
pASKXho-R
NotI - 1147 - GC'GGCC_GC
HaeIII - 1149 - GG'CC
HaeIII - 1225 - GG'CC
HaeIII - 1258 - GG'CC
R
HaeIII - 3115 - GG'CC
lE
or i
va
Co
HaeIII - 3858 - GG'CC
HisT
HaeIII - 80 - GG'CC
NcoI - 101 - C'CATG_G
HaeIII - 309 - GG'CC
HaeIII - 1585 - GG'CC
A
m pR
HaeIII - 2677 - GG'CC
HaeIII - 2597 - GG'CC
HaeIII - 2330 - GG'CC
HaeIII - 1727 - GG'CC
B
Stabilized M.HaeIII sequence
Nucleotides
ATGGGCAGCAGCCATCATCATCACCATCATAGCAGCGGCCTGGTGCCGCGCGGCAGCTCCATGGCAAATTTAATTA
GTCTTTTTTCAGGTGCAGGGGGATTAGATCTTGGCTTCCAAAAAGCAGGATTTCGTATTATTGCCGCAAATGAATAT
GATAAATCAATATGGAAAACATATGAAAGTAATCATTCAGCTAAATTGATCAAAGGCGATATATCTAAAATTTCTT
CAGATGAATTCCCTAAGTGTGATGGAATTATTGGGGGGCCGCCTTGTCAATCTTGGAGTGAGGGGGGATCTCTTAG
AGGAATTGATGATCCTCGGGGCAAACTTTTTTATGAATATATTCGGATTTTAAAACAAAAAAAACCAAAATTCTTTC
TTGCCGAAAACGTTAAAGGAATGCTGGCTCAGCGTCATAATAAGGCTGTTCAAGAATTTATCCAAGAATTTGATAA
TGCTGGATATGATGTCCATATTATTTTGCTTAATGCGAATGATTATGGTGTAGCTCAAGATAGAAAACGTGTTTTTT
ATATTGGTTTTAGAAAAGAGTTAAATATAAATTATCTTCCACCCATTCCACATTTGATAAAGCCAACATTGAAGGAC
GTCATTTGGGATCTTAAGGATAATCCAATTCCAGCTTTAGATAAAAATAAAACAAATGGTAATAAATGTATTTATCC
TAATCACGAATATTTTATAGGATCATATTCAACAATTTTTATGAGTAGAAATCGTGTTAGACAATGGAATGAACCAG
CATTCACAGTTCAAGCATCTGGACGACAATGTCAATTACACCCCCAAGCTCCTGTAATGTTAAAAGTTAGTAAAAAT
TTAAATAAATTTGTTGAGGGAAAAGAACATTTATATCGCAGACTAACGGTTCGGGAATGTGCACGAGTGCAAGGAT
TTCCAGATGATTTTATTTTTCATTACGAAAGTTTGAATGATGGTTATAAAATGATCGGTAACGCTGTGCCTGTAAATT
TAGCATATGAAATAGCTAAGACAATAAAATCTGCATTGGAAATTCGTAAAGGTAATTAG
Amino acids:
MGSSHHHHHHSSGLVPRGSSMANLISLFSGAGGLDLGFQKAGFRIIAANEYDKSIWKTYESNHSAKLIKGDISKISSDE
FPKCDGIIGGPPCQSWSEGGSLRGIDDPRGKLFYEYIRILKQKKPKFFLAENVKGMLAQRHNKAVQEFIQEFDNAG
YDVHIILLNANDYGVAQDRKRVFYIGFRKELNINYLPPIPHLIKPTLKDVIWDLKDNPIPALDKNKTNGNKCIYPNH
EYFIGSYSTIFMSRNRVRQWNEPAFTVQASGRQCQLHPQAPVMLKVSKNLNKFVEGKEHLYRRLTVRECARVQG
FPDDFIFHYESLNDGYKMIGNAVPVNLAYEIAKTIKSALEIRKGN
C
HA-tag:
CTC GAG GGA GGG TAC CCA TAC GAT GTT CCA GAT TAC GCT TAG
L
E
G
G
Y P
Y
D
V
P
D Y
A
*