Influenza Reagent
Influenza anti A/Victoria/3/3/75 HA serum
NIBSC code: 76/547
Instructions for use
(Version 6.0, Dated 31/03/2008)
8. STABILITY
Reference materials are held at NIBSC within assured, temperaturecontrolled storage facilities. Reference Materials should be stored on
receipt as indicated on the label.
NIBSC follows the policy of WHO with respect to its reference materials.
1. INTENDED USE
Influenza antiserum reagent 76/547 is prepared in a goat for the single
radial diffusion assay of A/Victoria/3/75 antigens
2. CAUTION
This preparation is not for administration to humans.
The material is not of human or bovine origin. As with all materials of
biological origin, this preparation should be regarded as potentially
hazardous to health. It should be used and discarded according to
your own laboratory's safety procedures. Such safety procedures
should include the wearing of protective gloves and avoiding the
generation of aerosols.
Care should be exercised in opening
ampoules or vials, to avoid cuts.
3. UNITAGE
There is no unitage assigned to this material.
4. CONTENTS
Country of origin of biological material: United Kingdom.
The antiserum is prepared in goat and diluted in PBS before freeze drying
and processed for freeze-drying in 1ml volumes as described by
Campbell, P.J., Journal of Biological Standardisation, 1974, 2, 249-267.
5. STORAGE
-20°C
Please note: because of the inherent stability of lyophilized
material, NIBSC may ship these materials at ambient temperature.
6. DIRECTIONS FOR OPENING
Tap the ampoule gently to collect the material at the bottom (labelled)
end. Ensure ampoule is scored all round at the narrow part of the
neck, with a diamond or tungsten carbide tipped glass knife file or
other suitable implement before attempting to open. Place the
ampoule in the ampoule opener, positioning the score at position 'A';
shown in the diagram below. Surround the ampoule with cloth or
layers of tissue paper. Grip the ampoule and holder in the hand and
squeeze at point 'B'. The ampoule will snap open. Take care to avoid
cuts and projectile glass fragments that enter eyes. Take care that no
material is lost from the ampoule and that no glass falls into the
ampoule.
9. REFERENCES
None
10. ACKNOWLEDGEMENTS
None
11. FURTHER INFORMATION
Further information can be obtained as follows;
This material: [email protected]
WHO Biological Standards: Http://www.who.int/biologicals/en/
Derivation of International Units:
Http://www.nibsc.ac.uk/products/faq.asp
Ordering standards from NIBSC:
Http://www.nibsc.ac.uk/products/faq.asp
NIBSC Terms & Conditions: Http://www.nibsc.ac.uk/terms.html
12. CUSTOMER FEEDBACK
Customers are encouraged to provide feedback on the suitability or use
of the material provided or other aspects of our service. Please send any
comments to [email protected]
13. CITATION
In all publications, including data sheets, in which this material is
referenced, it is important that the preparation's title, its status, the NIBSC
code number, and the name and address of NIBSC are cited and cited
correctly.
14.
MATERIAL SAFETY SHEET
Physical and Chemical properties
Physical
Corrosive:
No
appearance: White
powder
Stable:
Oxidising:
No
Yes
Hygroscopic:
Irritant:
No
No
Flammable:
Handling:
See caution, Section 2
No
Other (specify):
Contains goat serum and sodium azide (0.05%)
Toxicological properties
Effects of inhalation:
Avoid inhalation
Effects of ingestion: Avoid ingestion
Effects of skin absorption:
Avoid contact with skin
Suggested First Aid
Side view of ampoule opening device containing an ampoule
positioned ready to open. 'A' is the score mark and 'B' the point of
applied pressure.
7. USE OF MATERIAL
No attempt should be made to weigh out any portion of the
material
Reconstitute the total contents of one amp with 1ml of distilled water.
Allow to stand for 5 minutes. For convential vaccines the reagent should
be used at 0.25ml/12ml agarose.
Inhalation:
Seek medical advice
Ingestion: Seek medical advice
Contact with eyes: Wash with copious amounts of water. Seek
medical advice
Contact with skin: Wash thoroughly with water.
Action on Spillage and Method of Disposal
Spillage of ampoule contents should be taken up with absorbent
material wetted with an appropriate disinfectant. Rinse area with an
appropriate disinfectant followed by water.
Absorbent materials used to treat spillage should be treated as
biological waste.
National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, EN6 3QG
WHO International Laboratory for Biological Standards, UK Official Medicines Control Laboratory
T +44 (0)1707 641000
nibsc.org
Page 1 of 2
15. LIABILITY AND LOSS
Information provided by the Institute is given after the exercise of all
reasonable care and skill in its compilation, preparation and issue, but
it is provided without liability to the Recipient in its application and use.
It is the responsibility of the Recipient to determine the appropriateness
of the standards or reference materials supplied by the Institute to the
Recipient (“the Goods”) for the proposed application and ensure that it
has the necessary technical skills to determine that they are
appropriate. Results obtained from the Goods are likely to be
dependant on conditions of use by the Recipient and the variability of
materials beyond the control of the Institute.
All warranties are excluded to the fullest extent permitted by law,
including without limitation that the Goods are free from infectious
agents or that the supply of Goods will not infringe any rights of any
third party.
The Institute shall not be liable to the Recipient for any economic loss
whether direct or indirect, which arise in connection with this
agreement.
The total liability of the Institute in connection with this agreement,
whether for negligence or breach of contract or otherwise, shall in no
event exceed 120% of any price paid or payable by the Recipient for
the supply of the Goods.
If any of the Goods supplied by the Institute should prove not to meet
their specification when stored and used correctly (and provided that
the Recipient has returned the Goods to the Institute together with
written notification of such alleged defect within seven days of the time
when the Recipient discovers or ought to have discovered the defect),
the Institute shall either replace the Goods or, at its sole option, refund
the handling charge provided that performance of either one of the
above options shall constitute an entire discharge of the Institute’s
liability under this Condition.
16. INFORMATION FOR CUSTOMS USE ONLY
Country of origin for customs purposes*: United Kingdom
* Defined as the country where the goods have been produced and/or
sufficiently processed to be classed as originating from the country of
supply, for example a change of state such as freeze-drying.
Net weight: 1g
Toxicity Statement: Non toxic
Veterinary certificate or other statement if applicable.
Attached: No
National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, EN6 3QG
WHO International Laboratory for Biological Standards, UK Official Medicines Control Laboratory
T +44 (0)1707 641000
nibsc.org
Page 2 of 2