293 CAT# INDEX Price( ) FBS-SA Origin / Classical Liquid Media (MDEM) 292 Classical Liquid Media (RPMI-1640 & MEM) 293 Classical Liquid Media (Ham’s F-12 IMDM, McCoy’s 5A & Other) 294 Buffers / Salt Solutions / Cell Culture Supplements 295 Cell Dissoclation Reagents / Speciality Water / Antibiotics 296 Mycoplasma Prevention, Detection & Elimination Kits 297 rHSA, Trehalose & Sucrose 298 CELLclone – Introduction CELLclone’s Complete line of Cell Culture Media, Sera, Antibiotics, etc. Product Description CELL CULTURE Innovation for Life Sciences Serum Classical Media Buffers & Supplements Salt Solutions & Dissociation Reagents Mycoplasma Prevention, Detection & Elimination Kits 2015-16 E-mail : [email protected] Call +91-11-45027000 ~ Fax: +91-11-25419631 294 FBS-SA Origin / Classical Liquid Media (DMEM) CAT# Product Description Price( ) CAT# Product Description Price( ) Enhance the growth rate for your cells through our premium quality FBS Product Code : CCS-500-SA-U Features 3 BSE/TSE free 3 Filtered through triple 0.1 micron filters 3 Tested on cell lines such as HeLa, L929, Sp2/0-Ag14, MRC-5 Country of Origin: South America CAT# best& pricaelity qu Product Description Price( ) Serums - South American Origin CCS-500-SA-U 500ml serum, South American Origin (*Price subject to availability) 7,875 Dulbecco’s MEM Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins, non-essential amino acids and certain essential trace elements were added, the bicarbonate concentration increased. The standard formula is with 1000 mg/L glucose, the “high glucose” variation with 4500 mg/L. Dulbecco’s MEM was originally developed for the culture of mice embryonic cells. Today it finds a broad range of applications for serum-free culture of normal and transformed mouse and chicken cells, eg. 1:1 with Ham’s F-12. CAT# Product Description Price( ) 2016-17 Animal Cell Culture, Classical Liquid Medias (DMEM) Call +91-11-45027000 ~ Fax: +91-11-25419631 CC3008.1L DMEM (HG), With high-glucose, and L-glutamine No sodium pyruvate, 1000ml 1,621 CC3008.05L DMEM (HG), With high-glucose, and L-glutamine No sodium pyruvate, 500ml 822 CC3004.1L DMEM (HG), With high-glucose, L-glutamine, and sodium pyruvate , 1000ml 1,552 CC3004.05L DMEM (HG), With high-glucose, L-glutamine, and sodium pyruvate , 500ml 827 E-mail : [email protected] 295 CAT# Product Description CC3009.1L DMEM (LG), With low-glucose, sodium pyruvate, and L-glutamine, 1000ml CC3009.05L DMEM (LG), With low-glucose, sodium pyruvate, and L-glutamine, 500ml CC3001.05L CAT# Product Description CC3096.05L DMEM with 4.5 g/L Glucose, Sodium Phosphate without L-glutamine, Phenol Red, 500ml 1,097 CC3022.1L DMEM/F12 1:1, with L-Glutamine, without HEPES, 1000ml 1,968 CC3022.05L DMEM/F12 1:1, with L-Glutamine, without HEPES, 500ml CC3021.1L DMEM/F-12, With L-glutamine and 15 mM HEPES, 1000ml CC3021.05L DMEM/F-12, With L-glutamine and 15 mM HEPES, 500ml 1,672 866 DMEM with High Glucose, without L-Glutamine, with Sodium Pyruvate, 1000ml 1,671 DMEM with High Glucose, without L-Glutamine, with Sodium Pyruvate, 500ml 856 RPMI-1640 RPMI 1640, With L-glutamine and 25 mM HEPES, 500ml RPMI 1640, With L-glutamine, 1000ml CC3013.05L RPMI 1640, With L-glutamine, 500ml CC3011.1L RPMI 1640, Without L-glutamine, 1000ml CC3011.05L RPMI 1640, Without L-glutamine, 500ml E-mail : [email protected] 922 1,697 896 1,556 827 1,654 888 AMEM, With L-glutamine, deoxyribonucleosides, and ribonucleosides, 500ML 1,036 CC3018.1L EMEM, With Earle’s salts and L-glutamine, 1000ml 1,568 CC3018.05L EMEM, With Earle’s salts and L-glutamine, 500ml 824 CC3016.1L EMEM, With Earle’s salts No L-glutamine, 1000ml 1,641 CC3016.05L EMEM, With Earle’s salts No L-glutamine, 500ml 1,037 CC3040.05L EMEM, With Earle’s salts, L-glutamine, and nonessential | amino acids, 500ml 1,941 CC3020.05L MEM/Alpha Modification without L-Glutamine, Ribo- and Deoxyribonucleosides, 500ml 1,014 Call +91-11-45027000 ~ Fax: +91-11-25419631 2016-17 CC3014.05L CC3013.1L 2,072 Minimum Essential Medium (MEM) CC3019.05L RPMI 1640, With L-glutamine and 25 mM HEPES, 1000ml 924 MEM is one of the most widely used of all synthetic cell culture media. MEM Alpha is a highly complex formulation, enriched with additional amino acids, vitamins, lipoic acid and pyruvate. It is highly valuable if supplemented with ribo- and deoxyribonucleosides. Originally used for growth of hamster kidney cells. MEM Alpha supports the growth and proliferation of bone marrow and amniotic cells, both in monolayer- and suspension-culture. Supplementation with serum or certain protein supports the culture. RPMI 1640 was developed as a modification of McCoy’s 5A medium and is based on RPMI 1630. RPMI 1640 uses a bi-carbonate buffering system and differs from the most mammalian cell culture media in its typical pH 8 formulation. Without or supplemented with serum or other certain proteins RPMI 1640 today represents the most widely applied culture medium for short as well as long term cultivation of many cell types, especially human T/B-lymphocytes (eg.72h PHA stimulation assay), bone marrow cells, hybridoma cells. CC3014.1L Price( ) Classical Liquid Media (RPMI-1640 & MEM) CC3001.1L Price( ) 296 CAT# Product Description Price( ) CAT# Product Description Price( ) Ham’s F-12 Medium is a modification of the complex Ham’s F-10 formula, containing amino acids, vitamins and trace elements at optimum concentrations. Originally it was developed for serum free culture of CHO-cells (Chinese Hamster Ovary), primary Rat Hepatocytes and Prostate Epithelial Cells. Ham’s F-12 is today the medium of choice for the clonal toxicity assay with CHO-cells (CHD-3 and CHL-1) and supports the growth of a variety of normal, fastidious and transformed cells with or without serum. CC3073.05L Ham’s F-12 (Kaighn’s Modification), With L-glutamine, 500ml CC3027.05L Ham’s F-12, With L-glutamine, 500ml CC3027.1L Ham’s F-12, With L-glutamine, 1000ml 1,074 947 1,938 IMDM McCoy’s 5A Iscove’s Modified DM is the most known and widely used modification of Dulbecco’s MEM. It is a completely defined, serum free medium, supplemented with albumin, transferin and lecithin, selenium, amino acids, vitamins and HEPES buffer, pyruvate and KNO3 instead of Fe(NO3)3. Iscove’s MEM was originally developed for rapid propagation of erythropoietic and hemopoietic bone marrow precursor cells in serum free media. It is ideal also for the hybridoma and the T-/B-Lymphocytes cell culture of both human and mouse samples. McCoy’s 5A Medium was created using Basal Medium 5A and is a nutritive substance-rich complete medium. McCoy’s 5A Medium was originally developed to investigate the amino acid requirements of Novikoff Hepatoma cells. Today this optimal developed medium gains a broad applications to support the growth of the most demanding primary and continuous cell lines, and also from biopsy tissues, eg. bone marrow, skin, kidney, omentum, adrenal glands, lung, spleen. CC3025.1L IMDM with L-Glutamine, HEPES, without Alpha-Thioglycerol, 1000ml 1,749 CC3026.05L CC3025.05L IMDM with L-Glutamine, HEPES, without Alpha-Thioglycerol, 500ml 1,001 McCoy’s 5A, With L-glutamine, 500ml 928 Others CC3094.05L L-15, With L-glutamine, 500ml 1,089 2016-17 Classical Liquid Media (Ham’s F-12 IMDM, McCoy’s 5A & Others) Ham’s F-12 Call +91-11-45027000 ~ Fax: +91-11-25419631 E-mail : [email protected] 297 CAT# Product Description Price( ) Dulbecco’s Phosphate Buffered Saline (DPBS), 1X, with Calcium, Magnesium, without Phenol Red, 1000ml 1,569 Dulbecco’s Phosphate Buffered Saline (DPBS), 1X, with Calcium, Magnesium, without Phenol Red, 500ml 831 CC3089.05L Dulbecco’s PBS (10x), 10X D-PBS without calcium and magnesium, 500ml CC3034.1L Dulbecco’s PBS, Without calcium and magnesium Sterile filtered, 1000ml CC3034.05L Dulbecco’s PBS, Without calcium and magnesium Sterile filtered, 500ml Price( ) 1,242 1,526 827 CC4029.025L DMSO (Dimethyl Sulfoxide), 250ml GX-FM-050 Cell Freezing Medium, 50ml 16,292 GX-FM-100 Cell Freezing Medium, 100ml 29,791 CC4020.010L HEPES Solution, 1M solution (2283 g/L HEPES) in purified water Sterile filtered, 100ml 944 CC4023.010L L-Glutamine Solution, 200 mM solution (292 mg/ml) in purified water Sterile filtered, 100ml 758 CC4017.010L Non-Essential Amino Acids Solution, 100X solution Sterile filtered, 100ml 780 CC4034.010L MEM Vitamins 100x Solution, 100ml 971 CC4035.010L Pluronic® F-68 Solution, 10% (100X), 10% solution (100X) , sterile filtered Insect Cell Culture Tested, 100ml CC4019.010L Sodium Bicarbonate Solution, 75% solution (75 mg/ml) in purified water Sterile filtered, 100ml 859 C4016.010L Sodium Pyruvate Solution, 100 mM solution (11 mg/ml) in purified water Sterile filtered, 100ml 859 CC4021.010L Trypan Blue Solution, 04% in Phosphate Buffered Saline, 100ml 8,376 1,226 1,939 With sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, cell culture tested. CC3030.1L Hank’s Balanced Salt Solution (HBSS), 1X, without Calcium, Magnesium, with Phenol Red, 1000ml 2,469 CC3030.05L Hank’s Balanced Salt Solution (HBSS), 1X, without Calcium, Magnesium, with Phenol Red, 500ml 901 CC3045.05L Hank’s Balanced Salt Solution (HBSS), 10X, w/Ca, Mg, w/0 Phenol Red & Sodium Bicarbonate, 500ml 1,624 CC3046.05L Hank’s Balanced Salt Solution (HBSS), 10X, w/o Ca, Mg, Sodium Bicarbonate, 500ml 1,588 CC3054.1L Hank’s Balanced Salt Solution (HBSS), 1X, w/ Ca & Mg, 1000ml 1,618 CC3054.05L Hank’s Balanced Salt Solution (HBSS), 1X, w/ Ca & Mg, 500ml CC3041.1L Phosphate Buffer Saline, 10X, w/o Calcium & Magnesium, 1000ml 1,841 CC3029.1L Phosphate Buffered Saline (PBS), 1X, 0067M PO4, without Calcium, Magnesium, Phenol Red, 1000ml 1,544 CC3029.05L Phosphate Buffered Saline (PBS), 1X, 0067M PO4, without Calcium, Magnesium, Phenol Red, 500ml 818 957 2016-17 E-mail : [email protected] Buffers / Salt Solutions / Cell Culture Supplements DPBS is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to irrigate, wash and dilute mammalian cells. Phosphate buffering maintains the pH in the physiological range. Calcium and Magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells CC3032.05L Product Description Supplements Buffers and Salt Solutions CC3032.1L CAT# Call +91-11-45027000 ~ Fax: +91-11-25419631 298 CAT# Product Description Price( ) CC5028.010L Trypsin 10X, 0.25% Trypsin in HBSS without calcium, magnesium, and phenol red Porcine Parvovirus Tested, 100ml 1,282 CC5025.010L Trypsin 1X, 0.25% Trypsin in HBSS without calcium and magnesium Porcine Parvovirus tested, 100ml 742 CC5026.010L Trypsin-EDTA Solution, 005% (1X), Contains 005% trypsin and 1mM EDTA in Hank’s Balanced Salt Solution without calcium and magnesium Sterile filtered Porcine parvovirus and mycoplasma tested, 100ml 805 CC5027.010L Trypsin-EDTA Solution, 025% (1X), Contains 025% trypsin and 1mM EDTA in Hank’s Balanced Salt Solution without calcium and magnesium Sterile filtered Porcine parvovirus and mycoplasma tested, 100ml 782 Price( ) Antibiotics CC3038.1L Purified Water, Cell culture tested Sterile Filtered, 1000ml CC3038.05L Purified Water, Cell culture tested Sterile Filtered, 500ml 909 CC3036.01L Water, MB Grade, DNase, RNase, Protease free Deionized, Distilled, 01 µm Sterile Filtered, 100ml 669 Call +91-11-45027000 ~ Fax: +91-11-25419631 Product Description Prevent microbial attack on your cells by using our antibiotic / antimycotic solutions Speciality Water 2016-17 Cell Dissociation Reagents / Speciality Water / Antibiotics Cell Dissociation Reagents CAT# CC4007.010L Penicillin-Streptomycin Solution (100X), Contains 10,000 units of penicillin and 10,000µg streptomycin in a 0.85% saline solution. Sterile solution, 100ml CC4003.010L Antibiotic-Antimycotic Solution (100X), Contains 10,000 units of penicillin, 10,000µg streptomycin, and 25µg amphotericin B in a 0.85% saline solution. Effective against bacteria, fungi, and yeasts. Sterile solution, 100ml 1,693 917 1,008 CC4006.002L G418 Sulfate Solution, 50 mg/mL, 20ml 6,107 CC4006.001L G418 Sulfate Solution, 50 mg/mL, 100ml 21,833 CC4009.050P G418 Sulfate, > 90% Purity, Powdered, 5g 13,740 CC4009.001P G418 Sulfate, > 90% Purity, Powdered, 1g 4,580 CC4002.001L Gentamycin Solution, 50 mg/mL, Contains 50mg/ml gentamicin. Sterile solution. 10x10ml 8,550 CC4002.001LS Gentamycin Solution, 50 mg/mL, Contains 50mg/ml gentamicin. Sterile solution. 10ml CC4005.002L Hygromycin B Solution, 50 mg/mL, 20ml CC4008.010P Puromycin 2HCl, Ultra-Pure Grade, Powdered, 100mg CC4004.005L Amphotericin B Solution, Contains 250µg/ml amphotericin B and 250µg/ml sodium deoxycholate. Effective against fungi and yeasts. Sterile solution, 50ml 1,008 CC4010.050L Kanamycin Sulfate, 50X, 50ml 1,115 945 5,343 13,374 E-mail : [email protected] 299 CAT# Product Description Price( ) Product Description Price( ) Product Overview DE-MycoX Mycoplasma Elimination Kit DE-MycoX Mycoplasma PCR Detection Kit Biological reagent that Detect 50 species integrates into the of Mycoplasma Mycoplasma membrane and compromises its integrity. Thereby killing it. DE-MycoX Mycoplasma Prevention Spray Easy Solution for Mycoplasma Free Lab equipments & lab surfaces. Selective inhibition Provide validity of test Broad Spectrum-Virucidal, of Mycoplasma results by internal control Bactericidal & Fungicidal activity. No cytotoxicity Ready-to-use, optimized PCR premix type 70% alcohol based disinfectant Effective – Mycoplasma eliminated in only one treatment. Used for the elimination of Mycoplasma and Acholeplasma in cell and virus cultures, and other biologicals Easy to use User friendly & safe. APPLICATIONS Eliminates Mycoplasma & Detect Mycoplasma in Acholeplasma in cell & various samples virus cultures, & other biologicals Prevents precious culture samples from getting contaminated with Mycoplasma Mycoplasma Detection, Elimination, Prevention Kits & Spray GX-E-100 DE-MycoX Mycoplasma Elimination Kit, 1 Kits 18,471 GX-E-200 DE-MycoX Mycoplasma Elimination Kit, 3 Kits 46,175 GX-E-400 DE-MycoX Mycoplasma Elimination Kit, 5 Kits 76,560 GX-E-0250 DE-MycoX Mycoplasma PCR Detection Kit, 25 Tests 27,407 GX-E-0500 DE-MycoX Mycoplasma PCR Detection Kit, 50 Tests 52,133 GX-P-1L DE-MycoX Mycoplasma Prevention Spray, 1L Mycoplasma Prevention, Detection & Elimination Kits Prevent, Detect & eliminate Mycoplasma contamination using our DE-MycoX sprays and kits CAT# 9,833 2016-17 E-mail : [email protected] Call +91-11-45027000 ~ Fax: +91-11-25419631 300 CAT# Recombinant Albumin (rHSA) Product Description Price( ) CAT# Product Description Price( ) rHSA / Trehalose & Sucrose Maintains colloid oncotic pressure and increases the circulating plasma volume Features Applications 3 3 3 3 3 3 3 3 3 Animal component free Virus / prions and other adventitious possibilities free Cost-effective Consistent performance Reduces usage of FBS or other animal derived albumins CAT# CC4081.01P Product Description Price( ) Recombinant Albumin (rHSA) Manufactured in a cGMP-compliant facility POR High Purity Pharmaceutical Excipients manufactured in cGMP-compliant facilities Trehalose Sucrose A non-reducing disaccharide consisting of two glucose molecules linked by an a, a–1,1 bond A non-reducing crystalline disaccharide made up of glucose and fructose High Purity Low Endotoxin (HPLE) Trehalose High Purity Low Endotoxin (HPLE) Used in the biopharmaceutical industry to stabilize proteins, lipids, and carbohydrates throughout the formulation and freeze/thaw life cycle of therapeutics Used in the biopharmaceutical industry to stabilize proteins, lipids, and carbohydrates throughout the formulation and freeze/thaw life cycle of therapeutics Widely used as a key component in cryopreservatives/freezing media to stabilize cell membranes and improve the recovery and robustness of cells used for bioproduction and cell therapy Widely used as a cryopreservative and media supplement in a variety of cell-based bioprocesses The stabilizing benefits of trehalose include but are not limited to: • Monoclonal Antibodies (mAbs) • Fusion Proteins • Antibody Fragments (fAbs) • Peptides • Stem cells • Vaccines Trehalose is available as USP-NF (United States Pharmacopoeia—National Formulary), EP (European Pharmacopoeia) and JP (Japanese Pharmacopoeia) CAT# CC-104-4 2016-17 Development of mammalian cell cultures Supplementation of serum-reduced / serum-free culture media Cell Storage and cryopreservation Formulation, vaccine, antibody and regenerative medicine research & development Product Description Price( ) a,a – Trehalose Dihydrate, High Purity POR (Low Endotoxin), USP/NF, EP, JP Available Pack Sizes : 1Kg, 5Kg Enquire about our Bulk Packs too Call +91-11-45027000 ~ Fax: +91-11-25419631 It is preferred to use trehalose in applications where solubility and/or viscosity issues are paramount The benefits of sucrose include but are not limited to: • Monoclonal Antibody(mAbs) stabilization • Media Supplementation • Antibody Drug Conjugate (ADC) stabilization • Peptide stabilization • Stem cell preservation • Vaccines stabilization Sucrose is available as USP-NF (United States Pharmacopoeia—National Formulary), EP (European Pharmacopoeia) and JP (Japanese Pharmacopoeia) CAT# CC-124-2 Product Description Price( ) Sucrose, High Purity (Low Endoxin), USP/NF, EP, JP CC-124-2 POR Available Pack Sizes : 1Kg, 5Kg Enquire about our Bulk Packs too E-mail : [email protected]
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