Certificate of Calibration
NIST Size Traceability from Thermo Scientific Corp.
Concentration Data from Kanomax FMT, Inc.
20 nm PSL Spheres
DESCRIPTION. The particle size standard detailed below, provides an accurate and traceable
size calibration for particle size analysis. They are part of a series of monodisperse polymer
microspheres with calibrated mean diameters traceable to the Standard Meter through the
National Institute of Standards and Technology (NIST). Diameters from a nominal 20
nanometers (nm) to a nominal 500 nanometers (nm) are available as calibrated by photon
correlation spectroscopy (PCS), transmission electron microscopy (TEM). The aqueous
medium has been prepared to promote dispersion and reduce clumping of the particles. The
certified mean diameter is traceable to NIST. The concentration is determined using an
UltraFine Atomization/Scanning Mobility Particle Sizing (UFA/SMPS) system.
Thermo Catalog Number: 3020A, Nominal 20 nm
Thermo Packaging Lot # 44438
KFMT Lot #1304, KFMT Expiration Date: May 24, 2016.
Size Data.
Certified Mean Diameter:
Standard Deviation:
Coefficient of Variation:
Hydrodynamic Diameter:
23 ± 2 nm
Not determined or specified
Not determined or specified
Not determined or specified
Concentration Data
Concentration
5 x 107 PSL spheres per mL at 20 nm and greater
Accuracy
± 10%
Lot to lot variability
± 5%
Concentration data valid through May 24, 2016.
Physical Data
Microsphere Composition:
Microsphere Density:
Index of Refraction:
Approximate Concentration:
No additional surfactant added
Store in a refrigerator
Avoid exposure to direct sunlight
Polystyrene
1.05 g/cm3
1.59 @ 589 nm
2.32 x 10-8 % as solids
David Blackford (President KFMT)