Photosynthesis Professor Andrea Garrison Biology 11 Illustrations ©2010 Pearson Education, Inc. Photosynthesis Photosynthesis 2 Photosynthesis • Converts solar energy to molecular bond energy • Occurs in chloroplasts of eukaryotes • Requires – Visible light for energy – Chlorophyll to trap the light – CO2 for carbon to build sugars – H2O for electrons to make bonds – NADPH to carry electrons Photosynthesis 3 Photosynthesis Photosynthesis 4 Photosynthesis Photosynthesis 5 Photosynthesis • Occurs in two stages – Light reactions • Occur on thylakoid membranes • Require light • Supply ATP and NADPH for Calvin Cycle Photosynthesis 6 Photosynthesis • Occurs in two stages – Light reactions • Occur on thylakoid membranes • Require light • Supply ATP and NADPH for Calvin Cycle – Calvin Cycle • Need no light, but will occur in light • Use products of light reactions • Produce carbohydrates Photosynthesis 7 Light Reactions • Use visible spectrum of sunlight Photosynthesis 8 Photosynthesis 9 Engelmann’s Experiment • What colors of light drive P/S? • O2 loving bacteria cluster around algae producing most O2 Photosynthesis 10 Light used by chlorophyll • Chlorophyll absorbs light in red/orange wavelengths and blue/violet wavelengths • Chlorophyll does not absorb green light – Plants look green Photosynthesis 11 Accessory Pigments • Carotenoids – Absorb blue-green light – Some pass the energy to chlorophyll – Some absorb excessive light energy which might damage chlorophyll Photosynthesis 12 Light Reactions • Pigments harvest light energy through excited e- s • Photon strikes e– Excited e- jumps to higher energy shell – e- falls back down • Releases energy Photosynthesis 13 Light Reactions • Sometimes an electron can get so excited it flies off the molecule and binds to an e- acceptor, then moves down an etransport chain Photosynthesis Energy released 14 Light Reactions • Two photosystems – Involve both excited e-s falling back to their original energy state and excited e-s leaving the molecule and moving down electron transport chains – Trap solar energy as excited e-s and use the energy to make ATP and NADPH for Calvin Cycle Photosynthesis 15 Photosystems •Cluster of pigment molecules •gather light energy in excited electrons •pass that energy between molecules and eventually to reaction center Photosynthesis 16 NADPH-Producing Photosystem • Photon hits photosystem pigment molecule • Energy eventually passed to reaction center • e- s leave reaction center, trapped by primary eacceptor Photosynthesis 17 NADPH-Producing Photosystem • Photon hits photosystem pigment molecule • Energy eventually passed to reaction center • e- s leave reaction center, trapped by primary eacceptor • e- s passed to NADP → NADPH Photosynthesis 18 Water-Splitting Photosystem • Photon hits photosystem pigment molecule • Energy eventually passed to reaction center • e- s leave reaction center, trapped by primary eacceptor • Water split to release e-s to replace those lost from reaction center Photosynthesis 19 Water-Splitting Photosystem • Photon hits photosystem pigment molecule • Energy eventually passed to reaction center • e- s leave reaction center, trapped by primary eacceptor • Water split to release e-s to replace those lost from reaction center • e- s passed down etransport to make ATP Photosynthesis 20 Summary of Light Reactions Photosynthesis 21 Calvin Cycle • Uses ATP for energy and NADPH for e-s to make bonds of sugar • Uses CO2 for atoms of sugar Photosynthesis 22 Calvin Cycle Photosynthesis 23 Calvin Cycle Photosynthesis 24 Calvin Cycle Photosynthesis 25 Calvin Cycle Photosynthesis 26 Photosynthesis 27
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