Monitoring and Early Detection
of Asian Longhorned Beetles,
Anoplophora glabripennis
Maya Nehme (1)
Melody Keena (2), Aijun Zhang (3), Kelli Hoover (1)
(1)PennState University- Entomology Department- UP, PA
(2)USDA- FS- Northeastern Research Station- Hamden, CT
(3)USDA- ARS- Beltsville, MD
Survey & Detection
http://www.aphis.usda.gov/plant_health/plant_pest_info/asian_lhb
Male-produced Pheromone
Male produced pheromone is a 1:1 (v/v) blend of
dialkylethers (MP)
4-(n-heptyloxy)butan-1-ol (alcohol)
4-(n-heptyloxy)butanal (aldehyde)
ALB pheromones
♂
Laboratory
bioassays
1- Tubes
2- Y-olfactometer
Male-produced
volatile pheromone
Trapping
Experiments
1- Greenhouse
2- China
Laboratory bioassays
150 x 10 cm
glass tube
I
12 V Fan
M
NS
Cotton
wick
Fine screen
Laboratory Results
Activity
Virgin
Mated
Males
A
C
Females
B
D
Alcohol
Virgin
Mated
Males
B
C
Females
A
D
Virgin females significantly attracted to 1 μg alcohol
Li
na
eu
lo
ca
ol
l
yp
tra
tu
ns
s
-P
oi
in
l
oc
ar
Li
ve
na
ol
lo
ol
ox
id
e
3C
cis
ar
-3
en
-h
e
ex
en
-1
-o
l
Pe
nt
an
M
ol
an
uk
a
oi
l
M
yr
ce
ne
ca
m
He
ph
xe
en
ny
e
la
ce
ta
te
Ve
rb
Be
en
on
nz
tra
yl
e
ns
ac
-C
et
at
ar
e
yo
ph
yl
le
ne
Y-olfactometer bioassays for plant volatiles
•14 plant volatiles tested
90
80
70
60
50
% Males
40
% Females
30
20
10
0
Combining plant volatiles to ALB
male-produced pheromone
60
80
70
50
60
40
50
% Females
30
% Males
% Females
40
% Males
30
20
20
10
10
0
0
MP
LMP
MP
MPLHEX
Greenhouse trapping
1- Test attractiveness of the male-produced pheromone
2- Test attractiveness of plant volatiles
3- Determine the most efficient trap design
Intercept PT
Screen sleeve
Curculio
Lindgren
funnel
Total trap catches/trap design
25
20
15
10
5
0
Intercept
Screen sleeve
Curculio
Lindgren funnel
Mean trap catches/week/lure
4.5
4
3.5
3
2.5
Intercept
Screen sleeve
2
1.5
1
0.5
0
Ctrl
MP
cis-3-hexen-1--ol Cis-3-hexen-1-ol
+ MP
Linalool
Trapping ALB
in China
1
2
3
Quang Xia
Qingtong Xia city
Ningxia
China
4
5
6
7
Traps
♂
C
C
A
AB
ABC
BC
♀
B
AB
A
B
AB
B
Summary
„
„
„
„
„
The alcohol is the major attractant in ALB maleproduced pheromone for females
Adding the aldehyde to the alcohol enhances
males’ attraction
Adding plant volatiles to MP increases
attractiveness
Screen sleeve traps and Intercept traps are the
best trap designs to be used.
MP can be used for monitoring ALB in the field
Thank you!!!
USFS Lab team- Ansonia
Vic Mastro, Dave Lance
Baode Wang, Joe Francese
ALB program – Amityville
Edwin Rajotte Ann Hajek
Larry Hanks Jacob Wickham
Wickham
Baker and Tumlinson’s labs at
Penn State
Funding:
¾Horticultural Research Institute
¾Alphawood Foundation
¾USDA-APHIS