Effects of Environment on Enzymes A Lesson on Enzymes Georgia Everett Tri-Central Community Schools Sharpsville, IN Lesson # 3 Edited by Melvin Limson, Margaret Shain, and Marsha Lakes Matyas Frontiers in Physiology is sponsored by the American Physiological Society (APS) and the National Center for Research Resources Science Education Partnership Award (NCRR SEPA, R25 RR025127) at the National Institutes of Health (NIH). Note: The activities that follow are DRAFT activities and have not yet been peer reviewed for content accuracy or pedagogy. The lesson plans and opinions in this report are those of the authors and do not necessarily reflect the opinions of any of the supporting institutions or the editors. The American Physiological Society 2011 Works in Progress © 2011 The American Physiological Society, Bethesda, MD Permission granted for workshop/classroom use with appropriate citation. ABOUT THE AMERICAN PHYSIOLOGICAL SOCIETY The American Physiological Society (APS) is a nonprofit organization devoted to fostering education, scientific research, and dissemination of information in the physiological sciences. The Society was founded in 1887 with 27 members. APS now has over 10,500 members. Most members have doctoral degrees in physiology and/or medicine (or other health professions). The APS supports a variety of educational activities, including programs and fellowships to encourage the development of young scientists at the undergraduate and graduate levels, with a particular focus on women and underrepresented minorities. ABOUT APS EDUCATION PROGRAMS The American Physiological Society (APS) has activities addressing educational and career issues at the K-12, undergraduate, and graduate/professional levels as well as continuing education for physiologists. At the K-12 level, the APS programs focus on providing resources and professional development for excellence in science education. At the undergraduate level, APS programs and fellowships encourage excellence in physiology education and the development of young scientists. Programs and activities are available for students and faculty and resources for undergraduate education and career exploration are provided. At the graduate level, APS programs and fellowships provide opportunities for student study, travel, and interaction with experienced physiologists. Educational materials offer tools to improve teaching and learning at both the graduate and professional levels. APS also provides awards, refresher courses, mentoring resources, and educational materials to promote continuing professional development for its members and excellence in physiology education for their students. Copyright © 2011 The American Physiological Society, 9650 Rockville Pike, Bethesda, MD 20814. All rights reserved. Recommended citation (book): Limson M, Shain M, and Matyas ML (Eds.). 2011 Works in Progress. Bethesda, MD: American Physiological Society, 2011. Recommended citation (lesson example): Reynolds, J. Did I Observe it or Infer it? A Lesson on Observations and Inferences. In Limson M, Shain M and Matyas ML (Eds.), 2011 Works in Progress. Bethesda, MD: American Physiological Society, 2011. Manufactured in the United States of America. This publication is protected by Copyright and permission should be obtained from the publisher prior to any prohibited reproduction, storage in a retrieval system, or transmission in any form or by any means, electronic, mechanical, photocopying, recording, or likewise. Permission is granted to duplicate for classroom or workshop use with appropriate citation. To obtain permission(s) to use material from this work outside of classroom or workshop use, please submit a written request to Director of Education Programs, American Physiological Society, [email protected], or call 301.634.7132. _____________________ References National Research Council (NRC). (1996). National Science Education Standards. Washington, DC: National Academy Press. Alignment and references to state science education standards as of 2010 are cited by the authors. The American Physiological Society APS 2011 Works in Progress © 2011 The American Physiological Society, Bethesda, MD Permission granted for workshop/classroom use with appropriate citation. Effects of Environment on Enzymes A Lesson on Enzymes Teacher Section PURPOSE The purpose of this unit is to help students understand the cellular environment needed for enzymes to work and how it relates to cell activity. OBJECTIVES Upon completion of this activity, students will be able to: explain that there is an ideal environment for enzyme activity. measure the effects of changes in temperature, pH, and enzyme concentration on reaction rates of an enzyme catalyzed reaction in a controlled experiment. use technology tools such as internet, digital thermometers and pH meters. work through the steps of the scientific method to design a lab experiment to test the effects of different environments on enzymatic activity. GRADE LEVEL 11th – 12th grade Geared towards Advanced Placement or Dual Credit Biology courses. PRIOR KNOWLEDGE Students should have an understanding of the scientific method as well as lab safety, lab techniques and proper use of lab equipment. Students should also have at least an introductory understanding of the macromolecules found in living organisms, homeostasis, pH and chemistry. TIME REQUIRED The unit should take approximately 2-3 weeks depending on the time allowed for lab completion. Labs can be broken up into sections to be completed over multiple days. INCLUDING ALL STUDENTS Students will be placed into groups based on learning styles and academic performance. Groups will also be co-ed. The lesson will give students opportunities to complete hands-on learning through designing and performing experiments. Visual learners will use the virtual labs to guide their understanding and knowledge of labs. Auditory learners will be able to listen to the presentations given to better aide in their understanding of enzyme activity. There can also be adaptations made to the lab to accommodate students with Individual Educational Plans and other learning needs. Finally, we will work with a variety of food samples from various cultures so that students from various areas can have a connection to the lab. QUESTIONS TO ASK ALONG THE WAY What are the four groups of organic macromolecules? Which group includes enzymes? How do enzymes work? Is one environment better for enzyme activity than another? What is the best environment? Why is understanding enzyme environment important for us? What is pH? How does this relate to enzymes? What items are needed for a controlled experiment? How can you test what environment is the best for enzyme activity? What would be the best way to represent your data? How can you expand on the information you have learned? Georgia Everett Kokomo, IN © 2011 The American Physiological Society, Bethesda, MD 2010 Frontiers in Physiology Online Teacher APS Works in Progress Permission granted for workshop/classroom use with appropriate citation. Effects of Environment on Enzymes A Lesson on Enzymes Teacher Section NATIONAL SCIENCE EDUCATION STANDARDS K-12 Unifying Concepts and Processes Systems, order, and organization Evidence, models, and explanation Change, constancy, and measurement Science as Inquiry Abilities necessary to do scientific inquiry Understandings about scientific inquiry Life Science The cell Matter, energy, and organization in living systems Science and Technology Abilities of technological design Understanding about science and technology History and Nature of Science Nature of scientific knowledge INDIANA STATE SCIENCE EDUCATION STANDARDS Principles of Biology B.1.4 Understand and describe that the work of the cell is carried out by the many different types of molecules it assembles, such as proteins, lipids, carbohydrates, and nucleic acids. B.1.5 Demonstrate that most cells function best within a narrow range of temperature and acidity. Note that extreme changes may harm cells, modifying the structure of their protein molecules and therefore, some possible functions. B.1.14 Recognize and explain that communication and/or interaction are required between cells to coordinate their diverse activities. B.1.15 Understand and explain that, in biological systems, structure and function must be considered together. B.1.16 Explain how higher levels of organization result from specific, complex interactions of smaller units and that their maintenance requires a constant input of energy as well as new material. B.1.17 Understand that and describe how the maintenance of a relatively stable internal environment is required for the continuation of life and explain how stability is challenged by changing physical, chemical, and environmental conditions, as well as the presence of disease agents. B.1.18 Explain that the regulatory and behavioral responses of an organism to external stimuli occur in order to maintain both short-and long-term equilibrium. B.1.19 Recognize and describe that metabolism consists of the production, modification, transport, and exchange of materials that are required for the maintenance of life. Georgia Everett Kokomo, IN © 2011 The American Physiological Society, Bethesda, MD 2010 Frontiers in Physiology Online Teacher APS Works in Progress Permission granted for workshop/classroom use with appropriate citation. Effects of Environment on Enzymes A Lesson on Enzymes Teacher Section MATERIALS Students will be either working alone or in a group of 3-4 students. Day 1: Concept mapping handout, one per person. Day 2: One egg, hot plate and pan for classroom demonstrations. Internet Treasure Hunt worksheet for each student and computer with Internet. Days 3-4: Computer with Internet connection and printer (teacher can go to the website ahead of time and print the handout for the students.) For each group: markers poster board or large construction paper Day 5-7: Enzyme Lab Worksheet for each student. Samples for testing: Materials needed for whole class: fresh chicken liver hot water bath chicken meat ice water bath apple Materials needed for each group: potato 10 mL 1M HCl solution (in dropper pita bread bottles to change the pH of the papaya testing solutions) plantain 10 mL 1M NaOH solution (in humus dropper bottles) beans 6 test tubes baking soda test tube holder plant leaf (such as 10 mL graduated cylinder Elodea) 20 mL of 3% hydrogen peroxide solution Only a small sample will straight-edge razor be used for most items so scissors you can get by with only forceps one of each item listed. With the chicken liver digital thermometers you may want to buy a stirring rod container to split up pH meter (or pH paper) among the class. Days 8-9: Computer with presentation software Day 10: Computer with projector for presentations SAFETY Some of the chemicals used in the lab are hazardous / poisonous. They are safe to use as long as proper caution and safe handling techniques are applied in the laboratory. Do not allow chemicals to enter mouth or small cuts or scratches on the hands. Do not inhale the vapors or powders or allow them to blow around. Always wash your hands carefully after an experiment and follow directions of instructor regarding safety precautions. Wear safety goggles while in the lab. Use thermal gloves or hot pads when handling hot objects. Never perform a lab experiment without your teacher’s permission. Georgia Everett Kokomo, IN © 2011 The American Physiological Society, Bethesda, MD 2010 Frontiers in Physiology Online Teacher APS Works in Progress Permission granted for workshop/classroom use with appropriate citation. Effects of Environment on Enzymes A Lesson on Enzymes Teacher Section PREPARATION Day 1 Students should create a concept map on a poster board to show their current AND understanding of enzymes. Within their concept map they should include the PROCEDURE following key terms: enzyme, macromolecules, protein, lipid, carbohydrate, nucleic acid, and cell. (Other key terms can also be included in this list.) The students will share their concept maps with the rest of the class. Day 2 The students, as a class, will be shown an egg to brainstorm what is known about the egg. (As one of the key ideas, students should be able to realize that the egg contains a protein.) Using a hot plate and pan, fry the egg and asked the students what happened. (They should come up with the idea that the heat from the hot plate changed the structure of the egg.) Pose the question, “Are there other things that can also change the egg’s molecular structure?” Also ask the students, “What are some other common proteins?” (Students should come up with the idea of enzymes by the end of the discussion.) Students can then complete the Internet Treasure Hunt to look at enzyme structure and function and what things can have an effect on enzyme activity. Days 3 and 4 Have students read the information about enzymes by completing the lab bench activity at the address listed below. In this laboratory, they will study some of the basic principles of molecular movement in solution and perform a series of activities to investigate these processes. http://www.phschool.com/science/biology_place/labbench/lab2/intro.html Students will then complete the web lab at the address listed below to access a web lab worksheet that looks at the reaction that occurs with catalase as well as examine the factors that affect the rate of reaction. http://biologycorner.com/worksheets/enzymelab_web.html Students should print out the worksheet at the website and fill it out. The teacher can decide whether to collect it or have students keep it for future reference. Finish Day 4 by dividing the students into groups to expand on the concept map from Day 1. They should include the terms from the previous activity and the following additional terms in their concept map: pH, temperature, denaturing, induced fit, catalase, hydrogen peroxide (H2O2). Have them complete the map on a poster that can then be hung up around the room. Day 5 Students are given the Enzyme Lab worksheet to complete Part A. This section of the lab will help guide the students through the process that they will be using in the following days. Once they have finished Part A they will begin designing their own lab using the materials given in Part A as well as some additional samples. Day 6 Students will design a lab following the framework on the student handouts listed in Part B to test which types of materials contain catalase. They may choose from the list of samples included on the student handout as well as have access to the same lab equipment used in Part A to complete their labs. Georgia Everett Kokomo, IN © 2011 The American Physiological Society, Bethesda, MD 2010 Frontiers in Physiology Online Teacher APS Works in Progress Permission granted for workshop/classroom use with appropriate citation. Effects of Environment on Enzymes A Lesson on Enzymes Teacher Section PREPARATION Day 7 Students will design the last lab following the framework given on the student AND handouts listed in Part C to investigate the effect cellular environment has on PROCEDURE cell activity. Days 8 and 9 Have students make a slide show of their lab results using presentation software. They should include an introduction about what they learned about enzymes before the experiment, their hypothesis, procedure and results, and they should also include a conclusion with data analysis. Day 10 Students are to present their projects to the class and assessed with the Group Presentation Rubric. Finish up the unit with a class discussion about enzymes and how they relate to proper functioning of the human body and homeostasis. WHERE TO GO Invite a researcher, physician, or science professional into the classroom to discuss the how enzyme activity and environment are important for proper FROM HERE functioning of the human body. Have students research common enzymes used in the digestive process and how the ideal environment for some enzymes are different. Students can do an experiment with digestive enzymes to find their ideal environment. A project can be completed by the students to find out which disorders are caused by enzyme imbalances and or structural differences of enzymes. SUGGESTIONS Students may be assessed according to the changes made in their concept maps to include the information gathered from the online lab experiences. FOR The maps can be graded based upon proper connections between ASSESSMENT macromolecules, enzymes, and environments. The online labs can be graded based on correct answers and interpretations on the lab data collected. Students can be assessed by their presentations and their ability to include all steps of the scientific method and their understanding of the role environment plays on enzymatic activity. Georgia Everett Kokomo, IN © 2011 The American Physiological Society, Bethesda, MD 2010 Frontiers in Physiology Online Teacher APS Works in Progress Permission granted for workshop/classroom use with appropriate citation. Effects of Environment on Enzymes A Lesson on Enzymes REFERENCES AND RESOURCES Teacher Section 1. BIO 114 Enzymes http://bioweb.wku.edu/courses/Biol114/enzyme/enzyme1.asp Describes the action of enzyme as well as the structure and how those can both be affected by pH as well as how hydrogen peroxide works. Accessed August 29, 2010. 2. Effect of Environment of Enzyme Activity http://academic.brooklyn.cuny.edu/biology/bio4fv/page/enz_and.htm Describes the effect that different environments have on enzyme activity. Accessed on August 29, 2010. 3. Enzyme Catalysis http://www.phschool.com/science/biology_place/labbench/lab2/intro.html Background introduction to enzymes and how they work within the AP Biology Enzyme Lab. Accessed August 29, 2010. 4. Enzyme Information Page http://www.anyvitamins.com/enzymes-info.htm Basic information about enzymes and their importance in the functioning of the human body. Accessed August 29, 2010. 5. Enzyme Lab http://biologycorner.com/worksheets/enzyme_lab.html Handout of one of the AP Biology required labs posted on Biology Corner. Accessed August 13, 2010. 6. Enzymes Web Lab http://biologycorner.com/worksheets/enzymelab_web.html An investigation that includes a web lab on enzyme activity posted on Biology Corner. Accessed August 13, 2010. 7. Internet Treasure Hunt Worksheet Tempate http://www.apsarchive.org/resource.cfm?submissionID=4962 Template for the Internet Treasure Hunt worksheet used for this lesson. Accessed April 7, 2011. 8. Reactions & Enzymes http://www2.estrellamountain.edu/faculty/farabee/biobk/BioBookEnzym.html Information about enzymes used in the Internet Treasure Hunt. Accessed August 29, 2010. Georgia Everett Kokomo, IN © 2011 The American Physiological Society, Bethesda, MD 2010 Frontiers in Physiology Online Teacher APS Works in Progress Permission granted for workshop/classroom use with appropriate citation. Effects of Environment on Enzymes A Lesson on Enzymes Student Section CONCEPT MAPPING ENZYMES Use the space below to create a concept map to show your understanding of enzymes. The concept map should include the following terms: Enzyme Macromolecule Protein Lipid Georgia Everett Kokomo, IN © 2011 The American Physiological Society, Bethesda, MD Carbohydrate Nucleic acid Cell Environment Cell membrane Active site Substrate Catalyst 2010 Frontiers in Physiology Online Teacher APS Works in Progress Permission granted for workshop/classroom use with appropriate citation. Effects of Environment on Enzymes A Lesson on Enzymes Internet Treasure Hunt Student Section Name: ______________________ Date: _______ This web info search will help you find information about enzymes and their function. You will be looking at pre-selected web sites to answer each question. It is important to not only find the information at the site, but also to consider who wrote the site, what their purpose is in writing it, and how credible (accurate) you think the information is. Question 1: What is an enzyme and what are the parts to an enzyme? Site 1 Enzyme Information Page http://www.anyvitamins.com/ enzymes-info.htm Website Title URL Site 2 Reactions & Enzymes http://www2.estrellamountain.edu/faculty /farabee/biobk/BioBookEnzym.html Who created this web site (organization, etc.)? Why did they create it? (check all that apply) How credible (accurate) do you think the info is? To provide factual information To influence the reader’s opinion To sell a product or service I’m not sure Very accurate Somewhat accurate Not very accurate I’m not sure To provide factual information To influence the reader’s opinion To sell a product or service I’m not sure Very accurate Somewhat accurate Not very accurate I’m not sure What did you learn? Georgia Everett Kokomo, IN © 2011 The American Physiological Society, Bethesda, MD 2010 Frontiers in Physiology Online Teacher APS Works in Progress Permission granted for workshop/classroom use with appropriate citation. Effects of Environment on Enzymes A Lesson on Enzymes Student Section Name: ______________________ Date: _______ Internet Treasure Hunt This web info search will help you find information about enzymes and their function. You will be looking at pre-selected web sites to answer each question. It is important to not only find the information at the site, but also to consider who wrote the site, what their purpose is in writing it, and how credible (accurate) you think the information is. Question 2: Website Title URL Why is environment important to enzyme activity? Site 1 Site 2 Enzyme Catalysis http://www.phschool.com/science/biolo gy_place/labbench/lab2/intro.html Effect of Environment of Enzyme Activity http://academic.brooklyn.cuny.edu/biol ogy/bio4fv/page/enz_and.htm Who created this web site (organization, etc.)? Why did they create it? (check all that apply) How credible (accurate) do you think the info is? To provide factual information To influence the reader’s opinion To sell a product or service I’m not sure Very accurate Somewhat accurate Not very accurate I’m not sure To provide factual information To influence the reader’s opinion To sell a product or service I’m not sure Very accurate Somewhat accurate Not very accurate I’m not sure What did you learn? Georgia Everett Kokomo, IN © 2011 The American Physiological Society, Bethesda, MD 2010 Frontiers in Physiology Online Teacher APS Works in Progress Permission granted for workshop/classroom use with appropriate citation. Effects of Environment on Enzymes A Lesson on Enzymes Student Section ENZYME LAB MATERIALS 1M HCl solution (in dropper bottle) 1M NaOH solution (in dropper bottle) 6 test tubes and test tube holder 10-ml graduated cylinder 20 ml 3% H2O2 solution (for each part) straight-edged razor blade scissors and forceps (tweezers) digital thermometer hot water bath ice water bath stirring rod ph paper or digital ph meter fresh chicken liver, chicken meat, apple, and potato (for Part A) PART A Procedure: Observing Normal Catalase Reaction 1. Create a data table to record the information in the following steps. (Hint: The questions with arrows should also be included in your data table.) 2. Place 2 ml of the 3% hydrogen peroxide solution into a clean test tube. 3. Using forceps and scissors cut a small piece of liver and add it to the test tube. Push it into the hydrogen peroxide with a stirring rod. Observe the bubbles. 4. What gas is being released? Throughout this investigation you will estimate the rate of the reaction (how rapidly the solution bubbles) on a scale of 0-5 (0=no reaction, 1=slow...5= very fast). Assume that the reaction in step 2 proceeded at a rate of "4." 5. Recall that a reaction that absorbs heat is endothermic; a reaction that gives off heat is exothermic. Now, feel the temperature of the test tube with your hand. Has it gotten warmer or colder? Is the reaction endothermic or exothermic? Is catalase reusable? 6. Place 2 ml of 3% hydrogen peroxide solution into a clean test tube and add a small piece of liver. What is happening in your test tube? 7. Pour off the liquid into a second test tube. Assuming the reaction is complete. What is this liquid composed of? What do you think would happen if you added more liver to this liquid? Test this and record the reaction rate. Explain your results (what is the liquid composed of?) 8. Add another 2 ml of hydrogen peroxide to the liver remaining in the first test tube. Is catalase reusable? Data: Design a data table to keep track of your results. PART B Design an Experiment #1: Using the list of materials from the previous lab as well as the food items from different cultures listed below, come up with a hypothesis, an experiment and put it in your lab notebook or on a separate sheet of paper. Question/Purpose: What items contain catalase? Materials: Same as Part A and include the following food samples: pita bread, papaya, and plantain, humus, beans, baking soda, and plant leaf (such as Elodea). Georgia Everett Kokomo, IN © 2011 The American Physiological Society, Bethesda, MD 2010 Frontiers in Physiology Online Teacher APS Works in Progress Permission granted for workshop/classroom use with appropriate citation. Effects of Environment on Enzymes A Lesson on Enzymes Student Section Hypothesis: This statement should include which items listed above you believe to contain catalase. When coming up with a hypothesis be sure to consider the information you have learned about enzymes and catalase. Procedure: Write out the steps you will use to test your hypothesis. You may use the previous lab to help guide you. Make sure that your procedure is detailed enough that someone can follow the exact steps that you followed. Teacher Approval: Get your procedure approved by your teacher before proceeding with the experiment. Data Table: Design a data table to collect your information. You will want to use one that was similar to the data table you designed in Part A. PART C Design an Experiment #2: Using the list of materials from the initial lab, come up with a hypothesis and an experiment to answer the questions below. Question: What effect do environments have on enzyme activity? Materials: You may choose from the same list of materials listed in Part A. Hypothesis: Your hypothesis should suggest which type of environment you think the enzymes will work the best. You should include what you expect to see as you change the temperature as well as the pH that will be best for catalase. Procedure: Using the materials come up with a procedure to test the effect of environment on enzymes. You are to change the environment by adjusting the pH of your hydrogen peroxide by adding HCl or NaOH to the sample or changing the temperature of your sample using the hot and cold water baths. Make sure your procedure is detailed enough that it can be reproduced by someone else. Teacher Approval: Make sure to get your procedure approved by your teacher before continuing. Data Table: Design a data table to collect your information. Analysis of Data: Look at all of the data collected in all three experiments and summarize your findings. Sources of Error: What were some sources of errors that occurred in your experiment? How these errors might affect your overall results? Conclusion: How does the data collected tie into the other information learned about enzymes? Were your hypotheses supported or rejected? What new questions can be asked based upon the information you gathered? Georgia Everett Kokomo, IN © 2011 The American Physiological Society, Bethesda, MD 2010 Frontiers in Physiology Online Teacher APS Works in Progress Permission granted for workshop/classroom use with appropriate citation. Effects of Environment on Enzymes A Lesson on Enzymes Student Section GROUP PRESENTATION Your group is to make a slide show of your lab results using presentation software. The presentation should include an introduction about what was learned about enzymes before the experiment, your hypotheses, summary of the procedures used, organized data and observations, an explanation of the data and a conclusion. The rubric located below will be used to evaluate your group’s work and understanding of the effect environment has on enzyme activity. Category Introduction Hypothesis Development Procedure / Experimental Design Data Collection Conclusion / Summary Presentation Grammar / Spelling 4 3 Summarizes all information learned in treasure hunt; information is accurate & detailed; relates to the problems stated in the lab. Independently developed a hypothesis wellsubstantiated by class information & observation of similar phenomena. Procedure was outlined by clearly identifying major components of experimental design: Independent & dependent variables, control, constants. Data was collected several times. It was summarized, independently, in a way that clearly describes what was discovered. Students provided a detailed conclusion clearly based on the data and related to previous class information & the hypothesis statement. All group members participated in the presentation. Summary of experimental design & findings was clearly presented. Summary lacks some details even though it relates to the problems in the lab; information is accurate. Summary is about topic that was study but does not include previous activities & labs. Summary lacks many details about the topic and/or is not about the topic being studied. Independently developed a hypothesis somewhat substantiated by class information & observation of similar phenomena. Procedure was outlined by identifying major components of experimental design: Independent & dependent variables, control, constants. Independently developed a hypothesis somewhat substantiated by class information or observation of similar phenomena. Procedure was outlined by identifying major components of experimental design, but some confusion about set-up exists. Needed adult assistance to develop a hypothesis. Data was collected more than one time. It was summarized, independently, in a way that clearly describes what was discovered. Students provided a somewhat detailed conclusion clearly based on the data and related to the hypothesis statement. Data was collected more than one time. Adult assistance was needed to clearly summarize what was discovered. Data was collected only once and adult assistance was needed to clearly summarize what was discovered. Students provided a conclusion with some reference to the data and the hypothesis statement. No conclusion was apparent OR important details were overlooked. All group members participated in the presentation. Summary of experimental design & findings were presented. Some group members participated in the presentation. Summary of findings was presented. Some group members participated in the presentation. Presentation was vague or confusing. There were no spelling or grammar errors throughout the presentation. There were 1 or 2 spelling or grammar errors throughout the presentation. There were 3 or 4 spelling or grammar errors throughout the presentation. There were 5 or more spelling or grammar errors throughout the presentation. Georgia Everett Kokomo, IN © 2011 The American Physiological Society, Bethesda, MD 2 1 Procedure that was outlined was seriously incomplete, even after adult feedback had been given. 2010 Frontiers in Physiology Online Teacher APS Works in Progress Permission granted for workshop/classroom use with appropriate citation.
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