Laboratory safety and management
Department of Molecular Biosciences,
Wenner-Gren Institute (MBW)
2013
I.
Welcome to MBW
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II.
Responsible for safety
General regulations
In case of emergency
Important telephone numbers
Aspects of safety and protection
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10
General
KLARA
Guidelines for laboratory work with chemicals
Health effects and exposure
Toxics chemicals
Regulations for work with combustible goods and organic solvents
Waste
IV. Working with radioactive substances
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7
General considerations
Points of order
Working alone in the lab
Equipment to be used for personal protection
o Eyes
o Hands and skin
Air hygiene
III. Handling of chemicals
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Radioisotope practice
General and legal guidelines for working with radioactive isotopes
Code of practice for isotope work
Radioactive waste disposal
Handling/storage
Liquid radioactive waste and scintillation liquids
Liquid waste
Dry waste
Radioactive substances in the form of sealed radiation sources
Plan your waste handling according to SSMFS 2010:2
Hand-over/transport
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V.
Working with bacteria, virus and cell cultures
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VI. Working with animals
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VII. Instruments
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General
Centrifuges and rotors.
VIII. Waste disposal guide
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Laboratory glassware.
Contaminated laboratory glass.
Radioactive waste
Hazardous waste
IX. Information concerning accidents
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X.
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General
Eye damage
Skin damage
Inhalation of toxic gases and chemicals
Ingested poisons
Injury caused by electricity
Shock
How to induce vomiting
Automated external defibrillator (AED)
Report all accidents/incidents to SAMIR
Useful legislations and lists
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Provisions and general recommendations from the Swedish work
environment authority (AFSar från Arbetsmiljöverket)
Swedish radioactive safety authority
The Swedish board of agriculture (Jordbruksverket)
Webportal för genetikmyndigheter
Medical products agency (Läkemedelsverket)
Swedish poisons information centre (Giftinformationscentralen)
Swedish civil contingencies agency (Myndigheten för
samhällsskydd och beredskap)
Regulations that apply to Stockholm University
o SAMIR
o Avfall och avfallsrutiner/ waste management procedures
o KLARA Inventory system for chemical products
Swedish chemical inspectorate (Kemikalieinspektionen)
Incase of emergency (Om något händer)
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XI. Information på svenska om gifter och olyckor
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XII. Form to assert that you will work according to MBW
regulations
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2013
I.
Welcome to the Department of
Molecular Biosciences, Wenner-Gren Institute
(MBW)
This instruction is intended to serve as guidance for the introduction of newcomers to
the department. The aim is to help you to rapidly learn about the general rules and
working routines in our department. The text outlines some rules but cannot be
regarded as complete. More detailed descriptions, rules and instructions can be found
in separate folders for instance regulations for radioactive work and working in the
animal house (see further in SOP ”Standard Operating Procedures”). It is the
responsibility of every individual to plan and conduct his/her work in a safe and clean
way without causing danger or disturbing other people or environment within or
outside the lab. Do not hesitate to ask other people in the lab, primarily your group
leader/supervisor if you need to discuss any details. This must be done BEFORE you
start your experiment or even before ordering new chemicals and other reagents.
Note: During the first week the newcomer has to study this document carefully and
get additional information from his/her group leader and the safety representative
(skyddsombud). Thereafter the newcomer has to sign a form that the introductory
information has been received and hand it in to the secretariat. The form is attached
this document.
Responsible for safety
The head of the department, Per Ljungdahl, has final responsibility for internal
control of the work environment at the department. It is possible, however, for the
head of department to delegate employer responsibility. If you have questions
regarding safety when you work the first person to ask is your group leader. You can
also ask one of the safety representatives for advice, see list of key persons below.
General regulations
Everyone working at MBW must ensure that they can distinguish the sounds of the
fire alarms, ventilation alarms and card-reader alarm. You must also know the
locations of fire extinguishers, first-aid equipments, emergency eye washes,
emergency showers, emergency escape routes, main switches for electrical power and
main stop-cocks for gas and water. Make sure you know where to find of a list of
telephone numbers to be used in case of an accident or emergency. A FIRST AID
AND CRISIS PLAN is posted beside the emergency exit plan and by all telephones.
Those who work in laboratories at MBW must have basic knowledge in laboratory
procedures. It is the responsibility of the supervisor to provide support and
information such that work can be carried out safely. It is the responsibility of the
supervisor to make new students, employees and guest researchers familiar with the
safety regulations.
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The supervisor is also responsible that instructions are supplied such that machines
and other equipments are used in the correct manner.
IN CASE OF EMERGENCY
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First-aid kits are available in the corridors and in many labs. Be sure you
know were to find it.
Emergency showers and eye-flush devises are situated in most
laboratories.
Fire. An evacuation plan is posted in all corridors. Here, information
concerning evacuation routes, fire posts, hand-held fire extinguishers and
carbon dioxide extinguishers, blankets, is available.
Memorandum: Rescue, warn/ alarm, extinguish
Evacuation: Gathering on the field at the front of the Arrhenius
laboratories, outside G-salen.
Electricity. The main switch for the electricity located in the corridor for
each laboratory, must be turned off in case of fire.
Gas. The main regulator for the gas supporting each laboratory is located
in the pulp cabinet in the corridor. This must be turned off in case of fire.
IMPORTANT TELEPHONE NUMBERS
- Ambulance; 112
- Fire brigade; 112
- Police; 112
- Swedish Poisons Information Centre (emergency); 112
- Swedish Poisons Information Centre (no emergency); 33 12 31
- Health care contacts (Vårdguiden); 32 01 00
- Stockholm University alarm-phone; 16 42 00
- Major safety delegate at SU; 16 42 68
- Work Environment Engineer (skyddsingenjör); 16 22 51,
- Section for Public Safety at SU; 16 22 16 (daytime)
- Section for Public Safety at SU; 16 42 00 (after working hours)
Key persons
A group with representatives from each section of the department handles the safety
and security aspects. You will find the names of these persons on a separate sheet
titled “Key persons at MBW”.
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II.
Aspect of safety and protection
GENERAL CONSIDERATIONS
It is the responsibility of each individual to plan and conduct his/her works in a safe way
without causing danger or disturbing other people or environment within or outside the
laboratories. When introducing a new laboratory technique, the safety aspects must be
considered. This must be done BEFORE starting experiments or ordering new chemicals
or reagents that are labeled as hazardous for humans. Consult your group-leader and/ or
other lab-personnel already when planning the experiments.
Every person in the lab is responsible for:
- that reasonable order is obtained at the working-areas,
- that equipment is handled in a proper way,
- that the workspaces are kept free from unnecessary equipments and consumables,
- that the safety regulations are followed.
The floors in the laboratories must be kept free from all objects. Electric adaptor devices
are NOT allowed on the floors.
In order to assure good ventilation and the function of fume hoods, all windows of the
laboratory should be kept closed, also during summer.
In the absence of magnetic holder device, doors connecting fire cells must be closed.
It is not allowed to eat, drink or bringing foods into the laboratories.
POINTS OF ORDER
A.
Children
Children must not be taken into the lab area.
B.
Pets
Pets are not allowed at the university since many people are allergic.
C.
Rules of conduct
Discuss the local rules in the laboratory. If accidents occur or incidents that could have been
an accident report to the safety representative and to the incident/accident reporting link
SAMIR at the SU homepage.
D.
Working hours
Official working hours are 8-1640 in the wintertime, 8-16 in the summer (1st of may -15th
September). This is exclusive lunch; hence you work 8 hours and 10 minutes per day in the
winter and 7 hours and 30 min in the summer.
When planning your work it is important that you are not performing dangerous steps in an
experiment when you are alone in the lab. Always make sure that someone knows where you
are if you work odd hours.
E.
Lunch rooms
In order to keep the lunchroom nice and tidy everyone is assigned one week responsibility,
together with another person, for kitchen duty on rotating bases. This includes cleaning the
tables, running the dish washing machine and keeping the coffee machine working. Lab coats
are not allowed in the lunchroom.
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F.
G.
Smoking is not allowed indoors at the university
Food and drinks are not allowed in the laboratories
H.
Last person to leave the lab
After working hours the corridor doors are locked and alarmed. You have to make sure that
windows are closed, and that doors to the rooms are locked. Switch off the light and
instruments that unintentionally have been left on.
WORKING ALONE IN THE LAB
Undergraduate students, including those doing a Degree Project (“examensarbete”) are
not allowed to work in the laboratories unless someone else (teacher, PhD-student) in the
same research group is present. He/she can be in the lab or in the office.
When working alone in the lab after working hours and during weekends, you should
make sure that someone else in the Department knows that you are there. See
”Ensamarbete” (Working alone) AFS 1982:3
Hazardous work is not allowed when you are alone in the lab.
EQUIPMENTS TO BE USED FOR PERSONAL PROTECTION
Each person working in the laboratory shall find out where to get a laboratory coat (which
should be used when working in the lab), and where to place dirty laboratory coats. The
laboratory coats must NOT be used in common areas outside the laboratory.
Unless obviously unnecessary, the need for other equipment for individual protection,
e.g., gloves, full-face visor, and respiratory protection device, should be considered in
every situation.
EYES
Protective glasses are obligatory when there is a risk for explosion or splashes of poisons,
corrosives, genotoxic compounds. Use specific glasses adapted for work with an
unprotected UV-source when needed. (An Eye burn by UV light happens easily if you do
not protect you eyes at the UV-table AND IT HURTS!)
Contact lenses should not be worn when chemicals are handled, since gases and vapors
can become concentrated under the lenses. If a chemical splash occurs, the lenses make it
almost impossible to immediately irrigate the eyes thoroughly. Soft lenses are particularly
dangerous as they may gradually absorb vapors.
Locate the eye-showers, emergency showers and make sure you understand how to use
them.
HANDS AND SKIN
1. If chemicals come in contact with the skin, the most appropriate
action is nearly always to rinse with water.
2. Never handle chemicals directly. Use a spatula or similar tool.
3. Wash your hands thoroughly with soap and water after finishing work
and after every contact with chemicals.
4. Rubber gloves protect only against substances that cannot pass
through rubber for example aqueous salt solutions. Non-polar
substances can easily pass through rubber gloves, in which case there
is no protection. Solvent-resistant gloves can be purchased from SU-Butiken.
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Only use the gloves where you work. If you have contaminated gloves when you
touch door handles and this like will contaminate the next person that touch it with
bare hands. The opposite applies when gloves are used to protect from RNAse, the
gloves pick it up from the door handles and other things you touch.
AIR HYGIENE
1. All strongly smelling, volatile or poisonous chemicals must be handled in
a fume hood.
2. Rinse contaminated glassware in the hood before taking it out.
3. Take particular care when performing chromatography, the equipment
which often does not fit in a fume hood.
4. Check that the traps in the drains are filled with water. The drains
are ventilated outwards, and the negative pressure in the labs causes
unused traps to dry out, allowing labs to cross-contaminate one
another. Drains not regularly in use can be filled with a layer of
glycerol.
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III.
HANDLING OF CHEMICALS
GENERAL
A general strategy in the laboratory is that all chemicals are to be regarded as being
potentially harmful to health and should therefore be used with the proper precautions.
It falls on the individual investigator to get accurate knowledge about possible health
effects of the specific chemicals that he/she is handling. You shall be instructed by your
mentor how to handle chemicals in a correct manner. Aim for proper routines in the balance
area, in the laboratory and during transport.
For chemicals that are classified and labeled owing to their properties of being dangerous
to health or the environment, such information is available in the safety data sheets that
are enclosed with the chemicals on delivery. Folders with relevant safety data sheets are
available for the laboratories in each corridor. Information on a majority of the chemicals
used in the laboratory is available at www.miljo.su.se. Enter the database KLARA and
search the chemical. Information can also be obtained from several databases
(Classification List, Riskline, N-Class, Restricted Substances Database) at the website of
the Swedish Chemicals Inspectorate (www.kemi.se).
It is important to realize that lack of classification and labeling with respect to health
effects does not imply that a specific chemical is harmless. It might implicate that existing
toxicological data are not sufficient to meet current criteria for classification and labeling
used by the authorities.
KLARA (Inventory system for chemical products)
https://secure.port.se/alphaquest/app_su/pcmain.cfm
KLARAs main focus is to present information to users about chemical
hazards and to gather information about where chemicals are being used.
Additional to this you can also perform risk assessments in the system.
You can also use KLARA to search for chemicals at the department. You can also
find out if a chemical you would like to test before you buy your own is available
somewhere at SU.
Example: Ask your local KLARA person
Logon for everyone at MBW:
Username: mbw
Password: you receive the password on paper from xx at the secreterist once you have
signed the form where you assure that you will work according to the MBW
regulations.
Click on the English flag if you need an English version of KLARA and then
“Chemicals”.
From this window you may reach the chemical database, click on “Chemical
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Products”. In this database you are able to find safety information (MSDS)
about approximately 31 000 different chemical products. In order to get back to
the main menu, use the function “Back” in the left upper corner.
There is a separate search function for chemicals at our department.
Search by selecting “Inventories” from the main menu and then “Search
products”. Select group or in this case click on "Sök här" for "Inst. för
molekylär biovetenskap, Wenner-Grens institut" With this function you are able to
find the storage and amount for each registered chemical at your department.
To search for a product, it is most convenient to use the CAS number for
the product. The CAS number you will for instance find on Sigma's website:
http://www.sigmaaldrich.com
GUIDELINES FOR LABORATORY WORK WITH CHEMICALS
• Be aware of the increased risk for accidents and mistakes when routine work gradually
builds up a composite feeling of skillfulness, monotony and tediousness. A person
may become so familiar with the daily work that the possible hazards with handling
chemicals might be forgotten. Dangerous chemicals should be stored in fire resistant
lockable cabinets with continuous ventilation. Flammable compounds, oxidizing
compounds, toxic compounds, acids and basic compounds are stored separately.
• Do not store chemicals above head height, especially not corrosives.
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When handling of chemicals could result in contamination of the air by healthdamaging or explosive dusts, vapors, fumes or gases, the work should be carried out
in a fume hood.
Fume hoods cannot be used for permanent storage of chemicals, since the containers
obstruct the airflow.
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All bottles, flasks, tubes, beakers, etc. containing chemicals or preparations should be
properly and clearly labeled with content, name of user and date.
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Never return used chemicals to their original containers.
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Exsiccator under vacuum should only be opened in the fume hood.
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Avoid exposing chemicals and solvents to direct sunlight.
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Pipetting solutions by mouth is not allowed.
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Keep your hands away from mouth and face when handling chemicals.
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Wash your hands after handling chemicals or even better every time you leave the lab
to avoid contaminations in the offices and the lunch­rooms.
HEALTH EFFECTS AND EXPOSURE
Read the MSDS for your chemical before start using it to get information of its effects on
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you.
Chemicals causing health effects are assigned to one or several of seven categories depending
on which of the inherent health-damaging properties listed below they show:
* General toxicity after a single short exposure
* General toxicity after repeated or prolonged exposure
* Corrosive and irritant properties
* Allergenic or sensitizing properties
* Carcinogenic properties
* Mutagenic properties
* Properties toxic to reproduction
The strength of a health-damaging effect varies between chemicals. A person working in a
laboratory may be exposed to a chemical by:
* Ingestion
* Absorption through the skin
* Inhalation
* Injection
In order to prevent such exposures, laboratory work should be performed in compliance with
the guidelines below and, last but not least, with common sense.
TOXIC CHEMICALS
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Toxic chemicals should be stored in a locked cupboard.
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Wear gloves when you handle toxic chemicals. Note that ordinary rubber
gloves protect against polar substances, e.g. water solutions of salts, but
they do not protect against apolar substances that can penetrate rubber.
Special, solvent-resistant gloves can be obtained at SU-butiken.
•
Toxic chemicals should be handled in a fume hood. This is important not
only for volatile substances, but also for chemical powders that produce
fine dust particles.
•
Do not expose other people to toxic chemicals! If you spill something,
clean up after yourself immediately!
•
Acrylamide and bisacrylamide are very toxic and may cause allergy. Avoid
handling acrylamide as a powder; aqueous stock solutions or preweighed aliquots are
available from many manufacturers. Acrylamidewaste should be polymerized.
•
Ethidium bromide is a powerful mutagen. Protective gloves must be used and
investigator must be extra careful to avoid contamination when working with it.
Solutions or gels containing more than 0.1μg/ml should be disposed in specially
marked containers and handled as hazardous waste.
•
Phenol. Always use protective gloves and work in the fume hood. Phenol solutions
and contaminated tubes, tips, etc are disposed in specially marked wasted bottles
that are sent for destruction.
A list of carcinogenic chemicals and other health hazardous chemicals is found in AFS
2011:19 pp 24-26. Chemicals in group A must not be handled. For group B chemicals special
permission is required. Whenever handling a chemical in group C (carcinogens which have
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threshold limits) make sure you know how to work with them and that there is a riskevaluation for your usage of the chemical.
Waste containing toxic chemicals (e.g., heavy metals, phenol, acrylamide,
ethidium bromide) must be delivered to the store on floor 2, (KÖL M212) as hazardous
chemical waste (Miljöfarligt avfall). For details see the Waste section.
REGULATIONS FOR WORK WITH COMBUSTIBLE GOODS AND
ORGANIC SOLVENTS
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Combustible goods are labeled with a flame symbol.
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Exercise great care.
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Handling combustible goods should be carried out in a fume hood if possible.
Ensure that no sources of ignition are present (electric equipment that may give rise
to sparking or gas that may explode if ignited by a spark).
•
When pouring combustible liquids (such as ethanol) outside a fume hood, make
sure that no sources of ignition are present within a radius of 0.5 meter.
•
The maximum quantity of combustible goods that may be stored in each laboratory
is 10 lfor everyday use. Try to minimize the volume as much as possible.
•
Combustible goods that must be stored in a refrigerator or freezer are to be stored
in aspark-protected refrigerator/freezer. There is one for all at MBW located in
F343 flera?.
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The maximum volume of combustible goods that may be freely handled in a fume
hood is 100 ml.
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Gas piping used with the coal gas supply must have clamps fitted at both ends.
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Gas cylinders must be anchored.
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Do not use spray bottles for 70% ethanol (due to the risk of aerosol formation).
Use instead paper with a wash bottle.
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The amount of organic solvents on the lab benches (e.g. for daily
consumption) should be kept as low as possible. 50 l flammable solvents per
“fire cell” (brandcell) are allowed to be stored in the specially designated,
ventilated cupboards.
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WASTE
Stockholm Water allows the following chemicals to be
flushed down in the fume hood at a rate of 1 liter per lab and day.
Acetone
Acetonitrile Acetic acid Ammonia
Formaldehyde Glycerol
Rinse carefully with (plentiful) water afterwards!
More information of other liquids that can be flushed down the drain can be found in
“Utsläppslistan, updated 120207”. You find it in the binder “Lab Routines” found in
every laboratory.
The following chemicals have to be turned in for destruction:
Iodopax.
Ether or petroleum ether. Furthermore all ether waste is to be tested for
peroxides before delivery to the waste store due to the explosive risk and
Phenol and chloroform.
Other solvents should be delivered to the waste store room in glass
bottles or metallic waste bins labeled with an appropriate, explanatory
label.
How to handle organic solvents:
All flammable solvents should be handled in the fume hood.
Most solvents penetrate ordinary rubber gloves so use special protective
gloves that can be bought from SU-butiken.
For more detailed information read the information in the chemical data base KLARA
and/or ask the safety representatives at the Department.
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IV.
WORKING WITH RADIOACTIVE SUBSTANCES
Working with ionizing radiation (SSI FS 2000:7,8,9) and radioactive isotopes, such as 32P,
14
C, 35S and tritium (3H), requires strict precautions and all work is regulated by
“Strålskyddslagen (SFD 1988 nr 220” www.ssi.se). “Strålsäkerhetsmyndigheten, SSM” is
responsible authority. The applicant for permit for working with radioactive substances is
Stockholm University; the radiation safety expert is Mats Jonsson, [email protected] 087909123. However, yearly reports have to be handed in for each department concerning
the amounts and isotopes used at each department. At MBW the contact person is Robert
Csikasz.
All personnel working with radioactive materials or sources of such must take a
compulsory course in Radiation Safety administrated by Security Department. Before
permit to work with radioactive material is given you also have to pass a test. The
responsible person at SU for radioactivity courses is Mats Jonsson. When the next course
is given can be found on the SU home page. You must also attend one seminar per every
two years to update knowledge and keep the permit to work with radioactivity.
Anyone organizing laboratory work involving isotopes at the MBW department are
responsible for providing adequate handling information. Personnel that work with
isotopes must be informed and having taken instruction from the specific documents
regulating the experimental conditions under which experiments with isotopes can be
performed.
RADIOISOTOPE PRACTICE
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Safe use of radioisotopes is largely a matter of common sense and good
laboratory practice.
•
The rules for waste disposal are open to a limited degree of interpretation as,
for instance, whether something should be left to decay before disposal. It is
up to you to implement these rules intelligently.
•
It is your responsibility to make sure that you are aware of all regulations and
guidelines before working with radioisotopes.
•
The major stipulation of these rules is that radioisotope work is to be
contained within designated, and clearly marked, areas.
GENERAL AND LEGAL GUIDELINES FOR WORKING WITH
RADIOACTIVE ISOTOPES
Radioactive work is divided into four categories of toxicity based on
the nuclide involved (A-D), and three categories depending upon the
physical risks associated with the experiment (categories I-III). A full
list of nuclides is appended and can be found in the “Lab routines” binder.
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The common isotopes that we use fall into the following categories:
B – 125I
C – 35S, 14C, 32P, 33P
D – 3H
The physical categories can be briefly summarized as follows:
I
II
III
Work with dusts, gases or very high levels of specific activity
Work with liquids (solutions, analyses, animal experiments)
Simple liquid operations (e.g., withdrawal of an aliquot from a stock
solution) and closed systems.
As a general rule, no isotopes in category A are used (or allowed) in
the Department and no work in category I takes place; if either are
being considered then advice (and permission) must be sought. Consult Mats Jonsson
and/or xxx at MBW.
1. The following amounts of radioactivity (in mCi) may be handled at any
one time:
I
II
III
A
0.00135
0.0135
0.135
B
0.0135
0.135
1.35
C
0.135
1.35
13.5
D
0.135
1.35
13.5
For example, it is permissible to store 13.5 mCi of a category C isotope in
a single tube, since storage and simple withdrawal are class III
manipulations. The maximum amount that can be withdrawn and used in a
given class II experiment is then 1.35 mCi.
2. As much work as possible (in principle all) should take place in the
isotope labs. However, limitations of space makes this impractical, not
least because of the central role of isotopes in biochemical research.
Therefore, work in categories II and III and with nuclide types C and D
(shaded above) may take place in “normal” labs, but the following rules
must be obeyed:
i. The amount concerned must be small (in the μCi range).
ii. All withdrawals from stock solutions must take place in the isotope
labs.
iii. Work with radioisotopes in the “normal” lab must be contained in an
area which may not be used for other purposes at the same time, and
which must be clearly marked (“radioactivity warning symbol” tape
or signs).
iv. All the other rules regarding isotope usage must also be adhered to.
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CODE OF PRACTICE FOR ISOTOPE WORK
1. The Department has three? labs designed and designated for use
with radioactive isotopes – F441, F3xx , and F219.
2. Accidents and other incidents concerning isotope work must be
reported through SAMIR (se section SAMIR) as soon as possible.
3. Personal dosimeters must be worn at all times, even if you are not
personally using high-energy sources. Did that apply at the old departments?
4. Physical shielding (lead metal or, where appropriate, Plexiglas) must be used with
all high-energy beta sources and with all gamma/X-ray sources. This includes
adequate (sideways and upwards) shielding when transporting material.
5. Working areas must be monitored during and at the end of experiments. If no
appropriate monitor is available (e.g. for 3H work), the area should be wiped with
swabs and these checked in a scintillation counter. Contamination must be dealt with
immediately.
6. Good laboratory practice, including the use of automatic pipettes, lab coats, gloves,
safety glasses and masks where appropriate, should be followed at all times. Gloves,
safety glasses and decontamination equipment must be available in the isotope labs at
all times. Hands must be washed and your person and clothing monitored for
contamination at the end of a session. Gloves must be discarded in the isotope labs.
7. Never work directly on the bench, but use a protective layer of plastic-coated
absorbent paper. This should be disposed of as radioactive waste, and changed
regularly or whenever necessary.
8. Work as much as possible in a fume hood. Always work in a fume hood when
using large amounts of radioactıve material of any type or where a significant risk of
dust or aerosol formation exists. All work with category B isotopes (such as 125I) must
be carried out in the isotope lab fume hoods.
9. All materials must be labeled with name, group, nuclide, chemical, activity, date
and a “radioactivity warning symbol”. All stock solutions must be stored in the
isotope labs.
10. Logs must be kept of all radioactive materials used. This should include recording
of withdrawal from stock and log sheets (see example attached) and should be kept in
the same isotope lab as the stock, in the folder provided.
11. Dry radioactive waste is to be divided into combustible and noncombustible
waste, and separated by isotope type. Radioactive waste must be correctly labeled
and be kept separate from other waste in appropriately shielded containers.
Scintillation waste is to be kept separate from other radioactive waste.
12. Spillages and contamination must be contained and dealt with immediately, using
appropriate decontamination solutions – for instance a strong phosphate solution for
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decontaminating 32P. All potentially contaminated surfaces, including the floor, must
be checked. Contaminated equipment (such as pipettes) must be decontaminated
immediately and thoroughly, and not left to decay.
Contaminated glassware is to be soaked overnight in decon solution,
rinsed and checked before return to normal circulation.
13. The cleaners do not clean the isotope labs – you do!
RADIOACTIVE WASTE DISPOSAL
Consult the new SU Waste Management Manual, pp51-54.
http://www.su.se/polopoly_fs/1.100054.1347006074!/menu/standard/file/waste_management
_procedures_120726.pdf
HANDLING/STORAGE
Radioactive substances must be stored under lock and key so that they are not
accessible to unauthorized persons. The storage must be satisfactory in terms of fire
protection. The storage site must screen off so that the dosage rate does not exceed 20
μSv/h in areas where people circulate or no more than 2 μSv/h in areas used by
someone on a permanent basis.
LIQUID RADIOACTIVE WASTE AND SCINTILLATION LIQUIDS
For example, organic and environmentally hazardous/harmful solvents such as liquid
scintillation solutions must not be poured out in the slop sink for liquid radioactive
waste and must be packed in plastic or glass bottles placed in a waste container
together with a sufficient quantity of absorbent to prevent leaks.
The total activity of the waste washed out in drains from a laboratory during a
calendar month must not exceed ten times the activity stated in SSMFS 2010:2.
The waste washed out at one and the same time must not exceed the activity stated in
SSM FS 2010:2.
The same summation rule as for waste for combustion applies to releases to drains.
Each discharge must be washed down with plenty of water. At every discharge point
there must be a clearly visible sign saying that liquid radioactive waste may be
washed down the drain.
NOTE: Solvents containing NPE (nonyl phenol ethoxylate), such as scintillation
liquids like Optiphase ’Hisafe’ 2 are listed in Council Directive 2003/53/EC and must
not be discharged into any water system in the European Community and must always
be sent for final disposal by combustion.
Flammable scintillation solutions must always be packed in small plastic and glass
bottles with an aggregate volume of no more than 1 liter per waste container.
Absorbent must be added to the vessel; the quantity must correspond to a capacity to
absorb at least double the quantity (2 liters) of liquid.
For non-flammable liquid scintillation solutions, no more than 5 liters may be packed
in a waste container with a sufficient quantity of absorbent.
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Scintillation waste
Waste scintillation fluid is to be disposed of as described below.
The same rules apply as for dry radioactive waste. Consider separating this waste into
different categories for ease of disposal. Remember that one Bq/ml (the legal
definition) is 600 dpm for a vial with 10 ml of scintillant.
1. Aqueous scintillant with a radioactive content of < 1 Bq/ml of any
isotope or < 10 Bq/ml of 3H or 14C may be disposed of down the drains.
2. Aqueous scintillant with a radioactive content of > 1 Bq/ml of any
isotope or >10 Bq/ml of 3H or 14C is to be disposed of as radioactive
waste.
3. Organic scintillant with radioactive content of <1 Bq/ml of any isotope
or <10 Bq/ml of 3H or 14C is to be disposed of as scintillant waste.
4. Organic scintillant with radioactive content of >1 Bq/ml of any isotope
or >10 Bq/ml of 3H or 14C is to be disposed of as radioactive waste.
5. Scintillation waste must be kept separately from dry waste. Glass and
plastic vials should not be mixed.
LIQUID WASTE
In principle all liquid waste can be disposed of down the drains,
accompanied by a large volume of water, unless it should be treated as
chemical waste (or environmentally dangerous waste), in which case it
should be disposed of through the stores, as for solid waste. See SSMFS 2010:2 to
find out how much you can flush out per occasion and month.
Storage of short-half-life isotopes (e.g. 32P) for local decay before
disposal is permitted. It is possible to store these in lead chambers in the
radioactive waste store – talk to the store person for details.
Contact the person responsible for radioactivity at the department (currently xxx) for
more details on any of these matters.
DRY WASTE
1. Waste is to be sorted into combustible and noncombustible waste and
ideally also by isotope category (as above). Remember, however, that
although 32P and 14C (for instance) are in the same regulatory category
it may be more practical to separate them.
2. Waste must be properly labeled. Each container should have a single
radioactive waste label that indicates name, institution, phone number
and signature, and a single hazardous waste label, also correctly filled in.
All waste is disposed of via the stores on floor, room A205, (opposite SU-butiken).
Access to the radioactive waste storage area can only be obtained during working
hours and all deliveries must be recorded to store personnel. Sealable
bins for this purpose can be obtained from the stores; waste may be
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also be stored in cardboard hazardous waste boxes. Both must be
lined with a plastic bag.
3. Medium-activity waste is defined as that which contains more than 10
mCi of 3H or 14C, or which contains more than 1 mCi of an isotope with
t1/2 < 2 years, or which contains more than 0.1 mCi of an isotope with
t1/2 > 2 years (not counting 3H or 14C), and must be labeled as such.
RADIOACTIVE SUBSTANCES IN THE FORM OF SEALED RADIATION
SOURCES
Under the regulations (SSM FS 2010:2) sealed radiation sources with an activity
quantity of no more than 50 kBq may be sent for waste combustion. If there is the
slightest uncertainty about the activity of the sealed radiation source to be disposed of,
contact the University's radiation protection expert Mats Jonsson (08-790 9123,
[email protected]) or Mats Hansson at the Section for Safety [Sektionen för Säkerhet]
(08-16 2251, [email protected]).
PLAN YOUR WASTE HANDLING ACCORDING TO SSMFS 2010:2
You can find a general description of how waste shall be treated in the Radioation
Safety Manual available at every department.
It is important to document all handling of radioactive substances all the way to how
you pack your waste that in turned in or disposed of in other ways.
• Release to air- Should not give more than 10µSv/y to representive person.
• Release to sewar- Activity per month may not be more than 10* the value
given in the regulation. Activity per disposal may not be higher than the value
in the regulation.
• Waste for incineration – every package must not exceed the listed value.
• Packing and storage- The surface dose rate has to be given as well as an ID
number, name of sender, date and nuclide must be clearly labeled.
• Waste that is stored for decay should also be documented.
HAND-OVER/TRANSPORT
Radioactive waste in accordance with these restrictions can be deposited on
Thursdays at 10.30–11.00 in room A205 (opposite the SU Shop). The waste
contractor then transports the waste for destruction.
Radioactive waste that does not meet the restrictions according to these instructions
and SSMFS 2010:2 must be taken care of by Studsvik Nuclear AB. In such cases the
University’s radiation protection expert, and/or Ragnsells’ radiation protection expert
Erik Gustafsson (070-927 2539), must always be contacted since other requirements
under the ARD-S transport regulations and requirements concerning reporting to the
permit authority must also be complied with.
Läs ”Strålsäkerhetsmyndighetens föreskrifter om hantering av radioaktivt
avfall och utsläpp från verksamhet med öppna strålkällor SSMFS
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2010:2” (Aktivitetsgränser för utsläpp i avlopp och avfall till
förbränning). Detta för att kunna beräkna exakt vad som gäller för just din
radioaktiva isotops handhavande.
http://www.stralsakerhetsmyndigheten.se/Global/Publikationer/Forfattning/SSMFS/2
010/SSMFS-2010-2.pdf
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V.
WORKING WITH BACTERIA, VIRUSES AND
CELL CULTURES
Work with biological agents are regulated by the following “AFS:ar”
AFS 2005:01 – Microbiological risks, toxins and oversensitivity
AFS 2011:02/2000:5 – Use of genetically modified microorganisms
AFS 1986: - Handling of blood and blood products (only in Swedish)
These are directions from the Occupational health law (arbetsmiljölagen) and the
Environmental law (Miljöbalken) also governs how we are allowed to handle
biomaterial.
Most important is sterile technique that you have to be introduced to by your group
leader. Depending on the classification of the biological agent handling will decide
the stringency of the rules. But this applies to all:
Always use lab coat.
Agar plates and plastic tubes, plastic cuvettes and other disposable material shall be
autoclaved in special plastic bags (autoklavpåsar) and then left in the dishwashing
room on floor E5 or F5 in a special container labeled "Avfall/Waste". Thereafter they
can be treated as normal waste unless they contain a chemical or species that is not
destroyed by heat.
Sharp objects must be autoclaved separately.
Bacterial solutions and contaminated glassware should be autoclaved before
discarding or sent for washing.
Bacteriophages need treatement with iodopax (jodopax). Iodopax may not be poured
out in the waste but has to be turned in for destruction to M212 labeled appropriately.
The following antibiotics are not destroyed by autoclave treatment:
Blasticidin
Ciprofloxin
Enrofloxacin
Naldixin
Zeomycin
Zeozin
Has to be turned in for destruction and then burnt.
Vankomycin should not be used at all since it is the last resource against
mulitiresistent bacteria.
The above recommendations are from Karolinska Institute and the full list can be
found in the binder “Lab routines”.
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WORKING WITH VIRUSES
Special precautions must be taken when working with viruses. The rules vary
dependent on the type of virus and the type of experiment performed with the viruses.
Consult the instructions in Arbetarskyddstyrelsens Författningssamling AFS 1997:12
Biologiska ämnen. At the department Anders Nilsson 08-164549 is working with
bacteriophages and may help you on questions regarding viruses.
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VI.
WORKING WITH ANIMALS
License:
You are not allowed to conduct animal experiments without permission from the Ethical
Committee for Experimentation with Animals (djuretiska nämnden).
Such a license is applied for by the project leader and signed by an approved director for
the animal house (presently Dr Anders Jacobsson at The Department of Molecular
Biosciences, The Wenner-Gren Institute (MBW)
This license must be renewed every 3 years.
TO ENTER THE ANIMAL HOUSE YOU NEED A LABORATORY ANIMAL
TRAINING COURSE AND GAIN THE FELASA CERTIFICATE.
Ordering:
Order forms are available in the animal house. Fill in the form and give it to the staff
there.
All animals used in the animal house should be ordered in this manner.
Animals used in experimental research should be ordered from an approved breeder.
Labeling cages:
The cages should be marked with
a) The number of your approved license
b) Your name
c) The name of your department
In the animal house:
• Use protective clothing: lab coat, shoe covers and hair protection. This will
prevent allergenic substances (hair, etc.) from leaving the animal house.
• Furry animals should not to be taken to laboratories.
• Put dead animals in the freezer.
• Put radioactive dead animals in a special compartment in the freezer. Note on the
freezer: isotope, amount, date and name.
• Always clear up benches and cupboards after yourself.
• Always contact the animal house staff if you have any questions.
• Accidents:
• If you work with animals, you should be vaccinated against tetanus (stelkramp).
• One vaccination gives you protection for about 10 years.
• If an animal bites you and you do not know if your protection against tetanus is
satisfactory, contact the nearest health care center (Vårdcentral).
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VII.
INSTRUMENTS
For security reasons and in order to avoid damage to expensive equipment it is necessary that
the new collaborator contacts the person responsible for the particular instrument to get
proper instruction before first use. The responsibility list gives information about the
responsible person. The supervisor is also responsible that instructions are supplied such that
the machines and other equipment are used in a correct manner. Some instruments may
requires that you get a “drivers licence” before you are allowed to use it yourself.
Broken equipment, etc, that only needs minor repairs are to be reported directly to the
person responsible for the particular equipment.
CENTRIFUGATION AND ROTORS
Running costs for rotors and centrifuges are very high – better care means longer
lifetime and lower costs.
• Clean the rotors with mild detergents, rinse with distilled water and air dry after each
session of use.
• Keep the rotors in the cold-room, or the refrigerator in the centrifuge room.
• Never exceed the maximum speed of the rotor. For optimal life expectancy, to not
exceed 90% of maximum. Moreover, the permitted speed may be reduced when using
heavy solutions such as CsCl or if the rotor is old.
• Always fill in the log book. Note any fault in the log book and report it to responsible
• person.
• If a rotor is stuck or requires other attention, obtain help from responsible person. Do
not use force.
• If you are uncertain, then find out what to do BEFORE you do it.
• Never operate a swing-out rotor without all the buckets in place.
• NEVER switch buckets or lids between rotors, even apparently identical ones.
• Never operate a screw-on rotor (e.g., JA-14, JA-20) without a lid.
• Check over-speed disks before use.
• Never run without whole and lubricated O-rings.
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VIII.
WASTE DISPOSAL GUIDE
Consult the new SU Waste Management Manual:
http://www.su.se/polopoly_fs/1.100054.1347006074!/menu/standard/file/waste_management
_procedures_120726.pdf
Hazardous waste (riskavfall) should to be collected into the special cardboard boxes or
into sealable metal/plastic containers, obtainable from SU-butiken. Containers should be
lined with a heavy plastic bag. The container must be marked with the appropriate label,
correctly filled in. The labels can be found at M212 (from Ragnsells in 2013). You are
advised to have a small stock in each lab group. In addition, radioactive waste must have
an extra “radioactive waste” label. Delivery of waste to the stores can only be done
during working hours.
Chemical waste room (floor 2/KÖL M212):
Radioactivity waste room (floor 2/A205):
Wed/Fridays 10.30-11.00
Thursday 10.30-11.00
Contact:
Nina Hovberg, telephone 0761-01 79 19,
e-mail: [email protected]
Anders Lindholm, telephone 0709-27 45 22
e-mail: [email protected]
Hazardous waste includes the following:
Infectious material
Sharp objects (e.g., syringe needles)
Pharmaceuticals
Biological waste (see separate section)
Radioactive waste (see separate section)
Environmentally dangerous waste (miljöfarligt avfall) should to be collected and marked
as for hazardous waste, with the special label. The following are considered
environmentally dangerous:
Antibiotics (new rules; see SU Waste Manual)
Oil
Solvents (see separate section)
Paint/varnish
Glue
Strong acids and alkalis
Heavy metal waste (which includes cadmium, mercury, antimony, arsenic, barium,
beryllium, lead, cobalt, copper, chromium, nickel, selenium, silver, thallium, tin,
vanadium)
Cyanides
PCBs
Biocides
Laboratory waste
Laboratory glassware (deposit in room M212/KÖL)
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Green containers for non-contaminated laboratory glassware are marked as “Laboratory
glass”. There are also containers in F3, F4, F5 and E3, E5 corridors which are emptied on
are emptied on a regular basis. Of course the glass you throw in these containers have
been rinsed before deposition.
Contaminated laboratory glass is collected into cardboard boxes and must be marked
on the basis of the contamination concerned.
Batteries. There is a collection point outside the SU-butiken in the room where you also
can deposite fluorescent tubes.
Avfallshantering
Avfallsrutiner för Stockholms Universitet (REKOMMENDERAS!)
http://www.su.se/polopoly_fs/1.96199.1343374981!/menu/standard/file/avfallsrutin_120726.
pdf
För riskavfall i riskavfallsskartong märkt med riskavfallsetikett och dessutom, när det
gäller radioaktivt avfall, märkt med radioaktivitetsetikett ska följande anges:
Smittförande
Skärande, stickande
Kasserade läkemedel
Biologiskt avfall
Radioaktivt avfall
Till Miljöfarligt avfall (som skall lämnas i kartonger märkta med etiketten
miljöfarligt avfall) räknas följande gäller för följande kategorier:
Antibiotika (nya regler; se SU Avfallshantering)
Oljeavfall
Lösningsmedelsavfall
Färg- och lackavfall
Limavfall
Starkt surt eller alkaliskt avfall
Avfall som innehåller kadmium, kvicksilver, antimon, arsenik, barium, beryllium, bly,
cobolt, koppar, krom, nickel, selen, silver, tallium, tenn, vanadin eller zink.
Avfall som innehåller cyanid
Avfall som innehåller PCB Bekämpningsmmedelsavfall
Laboratorieavfall
Kontaminerat laboratorieglas lämnas i speciella kartonger till lab.glas-rummet (KÖL
M210b). Märk avfallsetiketten med kontaminerat labglas samt om det är biologiskt eller
kemikaliekontaminerat.
Tunnor för övrigt laboratorieglas finns uppställda på varje våningsplan och hämtas av
tekniska byrån när hämting beställs. Det får inte vara för smutsigt och illaluktande.Kan vi
fixa en sådan rutin precis som för returpapper?
Avlämnande av avfall
Miljöfarligt avfall
Lämnas i riskavfallsrummet (KÖL, rum M212). Avfallet får under inga omständigheter
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slängas i korridoren utanför förråden och måste vara korrekt uppmärkta.
Radioaktivt avfall
Lämnas på samma sätt som ovan i förråd för radioaktivt material.
Riskavfalls-rummet (vån. 2/KÖL M212):
Ons/Fredagar 10.30-11.00
Radioaktivitets-rummet (vån. 2/A205):
Torsdag 10.30-11.00
Kontakt: Nina Hovberg, telefon 0761-01 79 19,
e-mail: [email protected]
Anders Lindholm, telefon 0709-27 45 22
e-mail: [email protected]
Lysrör
Begagnade lysrör samlas i container mitt emot SU-butiken.
RISKAVFALL
Service som SU-butiken tillhandahåller för avfallet
Kartong för glasavfall med svart säck 6343-038
Kartong för miljöfarligt avfall med svart säck 6344-038
Dunk, PE, med lock 5l, för miljöfarligt avfall 6305-005
Dunk, PE, med lock 10l, för miljöfarligt avfall 6305-010
Dunk, PE, med lock 25l, för miljöfarligt avfall 6305-025
Riskafallshinken ersatt av denna:
Plastemballage för farligt avfall (gul), 50 lit 6342-050
Kanylburk, 0,5 liter 3900-001
Kanylburk, 1,5 liter 3900-002
Kanylburk, 2 liter 3900-003
Vi ska använda kartonger med plastsäck, som finns i SU-butiken. (Vissa kemikalier
skickas via Ragnsells till förbränning hos SAKAB)
Speciella etiketter som MÅSTE klistras på avfallsförpackningar ifyllda med innehåll,
institution och namn. Rullar med etiketter finns på sekretariatet, paketrummet, plan 4.
Riskavfall
Miljöfarligt avfall
Radioaktivt avfall
Glaskross (då ej glasavfallskartong används)
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IX.
INFORMATION CONCERNING ACCIDENTS
GENERAL
Information concerning all chemicals used in the Department is available in the
secretariat room, and in the KLARA register – ask your group representative How to use
KLARA see info at LEGISLATION AND LINKS. All employees must to know where
this information is kept. A general Classification List is also available at the Swedish
Chemicals Inspectorate (“KemikalieInspektionen”) but only in Swedish, see
http://www.kemi.se/Documents/Forfattningar/KIFS/K05_7.pdf
All employees must report all accidents and narrow escapes that occur in the department
to the SU-system SAMIR- Säkerhet - Arbetsmiljö - Miljö – Inrapportering (Safety-Work
environment-Environment-Reporting) (Man behöver ej logga in om man skall göra en
anmälan)
https://secure.port.se/alphaquest/app_su/pcmain.cfm?verkid="%23Z%24 Broken equipment, etc, that only needs minor repairs are to be reported directly to the
person responsible for the particular equipment.
IMPORTANT TELEPHONE NUMBERS:
In emergency situations, call: 112
For poison information (not emergency) call Swedish Poision Information Centre
(“Giftinformationscentralen”): 08-331 231 (office hours, otherwise 112 as above), see
also http://www.giftinformation.se/
Danderyd Hospital (in Mörby, closest hospital):
08-123 550 00
See also FIRST AID AND CRISIS PLAN, MBW (persons at MBW who have medical
training) at LEGISLATION AND LINKS
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HOW TO TREAT A PARTICULAR
ACCIDENT/INCIDENT
EYE DAMAGE
All chemicals should be regarded as potentially harmful. Always rinse with water if you
get anything in your eye.
The eye must be open when rinsing with water!!
Carefully remove contact lenses.
Acids and bases (not hydrogen fluoride)
Rinse eyes immediately with water for 15 minutes
Call the doctor
Hydrogen fluoride, bromide
Rinse eyes immediately with water for 15 minutes
Get immediate transport to a hospital (emergency: St Eriks Hospital is
the one for eye damage). During transport, the eye should be continuously rinsed using a
water bottle.
Always call first Emergency Department: 08-672 31 00 (advice given by Vårdguiden)
Blinding light (for example, UV)
Cover the eyes
Go to the hospital (emergency)
SKIN DAMAGE
All chemicals should be regarded as potentially harmful. Always rinse with
water if your skin has been exposed to any chemical or solvent.
Acids or bases (not hydrogen fluoride)
Rinse the skin with water and remove all contaminated clothing.
Call the doctor
Hydrogen fluoride
Rinse the skin immediately with water and remove contaminated clothing.
Rub the contaminated skin area with 2% calcium gluconate gel and
continue to rub until the pain diminishes or the doctor attends.
Bromide
Rinse the skin immediately with water and remove contaminated clothing.
Wash the wounded area carefully with 5% sodium thiosulfide.
Call for transport to a hospital.
Cyanide
If the affected person can breathe, administer oxygen. Remove all
clothing and shoes. If the affected individual cannot breathe, give
artificial respiration, with a respiration mask to protect the helper.
Transport the injured person immediately to a hospital. Forewarn the hospital!
Acrylamide
Rinse the skin immediately with water and remove contaminated clothing.
If necessary, call the doctor.
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Phenol
Rinse the skin immediately with water and remove contaminated clothing
Rub the contaminated skin area repeatedly with polyethylene glycol 400 or glycerol.
Call the doctor.
Burns
Minor burns are rinsed with water until the pain decreases. Do not puncture blisters.
Major burns are covered with sterile compresses and the victim is transported to the
hospital. Risk for shock exists (see shock).
INHALATION OF TOXIC GASES AND CHEMICALS
Note that some gases have a retarded effect!!! Always keep a person who has been
exposed to any toxic substances under observation.
Hydrochloric acid (HCl), Sulphur dioxide (SO2), Hydrogen sulfide (H2S), Nitrogen
dioxide (NO2), Chloride (Cl2)
Give oxygen and artificial respiration when needed.
Keep the exposed person warm and quiet, even if he/she does not show any symptoms of
injury at the time
All persons that have or have had any symptoms of injury should be transported to a
hospital.
Hydrogen fluoride (HF)
Make the exposed person blow his nose
Keep the injured person warm and quiet even if he/she does not show any symptoms of
injury at the time.
Call the doctor.
Bromide (Br2)
Keep the exposed person warm and quiet even if he/she does not show any symptoms of
injury at the time.
All persons that have or have had any symptoms of injury should be transported to a
hospital.
Carbon monoxide (CO)
Administer oxygen and artificial respiration when needed.
Call the doctor immediately or transport the exposed person to a hospital.
Cyanide
Give oxygen if the exposed person can breathe and remove all clothing and shoes. If the
exposed person cannot breathe give artificial respiration using a respiration mask to
protect the helper.
Transport the exposed person to a hospital immediately! Alert the hospital beforehand!
Acryl amide
Keep the exposed person warm and quiet.
Call the doctor.
Phenol
Keep the exposed person warm and quiet.
Call the doctor.
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INGESTED POISONS
All chemicals should be regarded as potentially toxic and the poison center
(Giftinformationscentralen) should always be contacted (112) if a chemical has been
consumed. In non-emergency situations, call
08-331 231 (during office hours) for information, see also:
http://www.giftinformation.se/
Larmvägar vid akuta förgiftningar
Vid förgiftningstillbud – ring 112 och begär Giftinformationen – dygnet runt.
Sjukvården har tillgång till speciella telefonnummer.
I mindre brådskande fall och för övriga frågor om förgiftningar – ring dagtid: 08-33 12
31, Giftinformationscentralen http://www.giftinformation.se/
Cyanide
Give oxygen if the exposed person can breathe and remove all clothing and shoes. If the
exposed person cannot breathe, give artificial respiration using a respiration mask to
protect the helper.
If the exposed person is fully conscious, induce vomiting.
Transport immediately to a hospital. Alert the hospital beforehand!
Bases, acids and ammonia
Do not induce vomiting!
Give milk or water (ca 500 ml) immediately to drink.
Transport the exposed person to a hospital immediately.
Salts of heavy metals
(e.g., arsenic, barium, lead, mercury)
Induce vomiting.
Transport the exposed person to a hospital immediately.
Phenol
If the person is fully conscious, give water to drink or a tablespoon of e.g. olive oil
immediately.
Call the doctor.
Acryl amide
If the person is fully conscious, rinse his/her mouth with water immediately and then give
a glass of water to drink.
INJURIES CAUSED BY ELECTRICITY
Electrical injury
Disconnect the electricity immediately by turning off the main switch or by causing a
short circuit.
Keep the injured person warm and quiet. Give artificial respiration if needed.
Transport the injured person to a hospital.
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SHOCK
Shock can arise from several causes, e.g., from burns, poisoning or major bleeding. The
condition is serious and implies that there has been a strong effect on the blood
circulation.
Symptoms
Paleness, cold sweat on the forehead and in the palms, ache and indisposition.
Treatment
Place the person in shock with his/her feet high and in a warm room free from drafts.
Keep the respiratory pathways free by placing the person in shock in a recovery position.
Keep the person in shock warm with blankets, etc.
Never give the person in shock anything to drink!!
Call the ambulance immediately.
Never leave the person in shock alone.
HOW TO INDUCE VOMITING
Vomiting must never be induced in an unconscious person or when acids, bases or
petroleum products have been consumed.
Vomiting is induced by giving the individual a glass of milk or water and then putting
two fingers in his/her throat as deep as possible and keeping them there until vomiting is
induced. Do not stop even if the person starts to choke.
AUTOMATED EXTERNAL DEFIBRILLATOR (AED)
With simple audio (in Swedish) and visual commands, AEDs are designed to be simple to
use for the layman, and the use of AEDs is taught in first-aid, CPR and AED training.
Application to [email protected] , 08-16 35 45
Here you will find your closest Defibrillator (Hjärtstartare):
House E, entré floor 3
Map where you can find other defibrillators Campus Frescati:
http://www.su.se/polopoly_fs/1.116727.1357127459!/menu/standard/file/Karta%20Campus%
20Frescati.pdf
When you remove the defibrillator from the cupboard the alarm goes to the guards and to
the Security section at SU. When you start D-HLR call 112 and send out a person to meet
the ambulance.
REPORT ALL ACCIDENTS/INCIDENTS TO SAMIR
SAMIR- Säkerhet - Arbetsmiljö - Miljö – Inrapportering
(Safety-Work environment-Environment-Reporting)
Log in is not necessary to write a report in SAMIR
https://secure.port.se/alphaquest/app_su/pcmain.cfm?verkid="%23Z%24 33
2013
X.
USEFUL LEGISLATIONS AND LINKS
Much of our rules are determined by, or connected to legislation as stated in a number of
”rule collections” from governmental departments and institutes. Below is listed a
number of the most important ones, and their web links (and most often with both English
and Swedish language variants).
Swedish Work Environment Authority (”Arbetsmiljöverket”)
http://www.av.se/
AFSar listade i nummerordning
http://www.av.se/lagochratt/afs/nummerordning.aspx
Provisions and General Recommendations in English (some AFSes in English)
http://www.av.se/inenglish/lawandjustice/provisions/
Kemiska arbetsmiljörisker AFS 2011:19
http://www.av.se/dokument/afs/afs2011_19.pdf
Chemical Hazards in the Working Environment AFS 2011:19
http://www.av.se/dokument/inenglish/legislations/eng1119.pdf
Artificiell optisk strålning AFS 2009:07
http://www.av.se/dokument/afs/afs2009_07.pdf
Kemiska arbetsmiljörisker
Checklista för riskbedömning
http://www.av.se/dokument/checklistor/checklista_riskbedomning.pdf
Hygienska gränsvärden AFS 2011:18
http://www.av.se/dokument/afs/afs2011_18.pdf
Occupational Exposure Limit Values AFS 2011:18
http://www.av.se/dokument/inenglish/legislations/eng1118.pdf
Innesluten användning av genetiskt modifierade mikroorganismer AFS 2011:02
http://www.av.se/dokument/afs/afs2011_02.pdf
Contained Use of Genetically modified Micro-organisms AFS 2011:02
http://www.av.se/dokument/inenglish/legislations/eng1102.pdf
Mikrobiologiska arbetsmiljörisker – smitta, toxinpåverkan, överkänslighet AFS
2005:01
http://www.av.se/dokument/afs/afs2005_01.pdf
Microbiological Work Environment Risks – Infection, Toxigenic Effect,
Hypersensitivity AFS 2005:01
http://www.av.se/dokument/inenglish/legislations/eng0501.pdf
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Gravida och ammande arbetstagare AFS 2007:05
http://www.av.se/dokument/afs/afs2007_05.pdf
Skyltar och signaler AFS 2008:13
http://www.av.se/dokument/afs/afs2008_13.pdf
Ensamarbete AFS 1982:03
http://www.av.se/dokument/afs/AFS1982_03.pdf
Belastningsergonomi AFS 2012:02
http://www.av.se/dokument/afs/afs2012_02.pdf
Arbetsplatsens utformning AFS 2009:02
http://www.av.se/dokument/afs/afs2009_02.pdf
Workplace design AFS 2009:02
http://www.av.se/dokument/inenglish/legislations/eng0902.pdf
Systematiskt arbetsmiljöarbete AFS 2001:01
http://www.av.se/dokument/afs/AFS2001_01.pdf
Systematic Work Environment Management AFS 2001:01
http://www.av.se/dokument/inenglish/legislations/eng0101.pdf
Första hjälpen och krisstöd AFS 1999:07
http://www.av.se/dokument/afs/AFS1999_07.pdf
First aid and crisis support AFS 1999:07
http://www.av.se/dokument/inenglish/legislations/eng9907.pdf
Webportal för Genetikmyndigheter (Regulation of Activities
involving GMO’s)
http://www.gmo.nu/
Universitetets rutin för hantering av GMO
http://www.su.se/medarbetare/service/krishantering/sakerhet/biosakerhet
Swedish Radiation Safety Authority (Strålsäkerhetsmyndigheten)
http://www.stralsakerhetsmyndigheten.se
Statens strålskyddsinstitut (SSI) var en svensk statlig myndighet som sorterade under
Miljödepartementet. 1 juli 2008 upphörde SSI efter att ha sammanlagts med Statens
kärnkraftinspektion (SKI) till den nya myndigheten Strålsäkerhetsmyndigheten.
SSMFS 2008:28; föreskrifter om laboratorieverksamhet med radioaktiva ämnen i
form av öppna strålkällor:
http://www.stralsakerhetsmyndigheten.se/Global/Publikationer/Forfattning/SSMFS/200
8/SSMFS2008-28.pdf
Strålsäkerhetsmyndighetens föreskrifter om grundläggande bestämmelser för skydd
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2013
av arbetstagare och allmänhet vid verksamhet med joniserande strålning SSMFS
2008:51
http://www.stralsakerhetsmyndigheten.se/Global/Publikationer/Forfattning/SSMFS/200
8/SSMFS2008-51.pdf
Strålsäkerhetsmyndighetens föreskrifter om hantering av radioaktivt avfall och
utsläpp från verksamhet med öppna strålkällor SSMFS 2010:2
(Aktivitetsgränser för utsläpp i avlopp och avfall till förbränning)
http://www.stralsakerhetsmyndigheten.se/Global/Publikationer/Forfattning/SSMFS/201
0/SSMFS-2010-2.pdf
Strålsäkerhetsmyndighetens allmänna råd om tillämpningen av föreskrifterna
(SSMFS 2012:4) om laser och intensivt pulserat ljus
http://www.stralsakerhetsmyndigheten.se/Global/Publikationer/Forfattning/SSMFS/201
2/SSMFS-2012-4.pdf
The Swedish Board of Agriculture (Jordbruksverket)
http://www.sjv.se/
Statens Jordbruksverks föreskrifter om befattning med animaliska biprodukter och
införsel av andra produkter, utom livsmedel, som kan sprida smittsamma
sjukdomar till djur och människor SJVFS 2009:6
Alla produkter från djurriket som inte är avsedda som livsmedel definieras som
animaliska bipodukter, d v s även proteiner >10 kD (exkl. Antikroppar, cellkulturer eller
andra proteiner som är affinitetsrenade och ligger i saltvattenlösning) och serum av animaliskt
ursprung.
http://www.jordbruksverket.se/download/18.26424bf71212ecc74b08000964/2009-006.pdf
Statens Jordbruksverks föreskrifter om innesluten användning av genetiskt
modifierade växter SJVFS 2007:29
http://www.jordbruksverket.se/download/18.26424bf71212ecc74b080001120/2007-029.pdf
Medical Products Agency (Läkemedelsverket)
http://www.lakemedelsverket.se/
Läkemedelsverkets författningssamling, föreskrifter om förteckning över narkotika:
http://www.lakemedelsverket.se/upload/lvfs/LVFS_2011-10.pdf
http://www.lakemedelsverket.se/upload/lvfs/konsoliderade/LVFS_2011_10_konsoliderad_to
m_2013_1.pdf
Läkemedelsverkets föreskrifter om kontroll av narkotika
http://www.lakemedelsverket.se/upload/lvfs/LVFS_2011-9.pdf
Swedish Poisons Information Centre (Giftinformationscentralen)
http://www.giftinformation.se/
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2013
Swedish Civil Contingencies Agency (Myndigheten för
samhällskydd och beredskap, tidigare Räddningsverket)
formellt Statens räddningsverk (SRV), var en 1986 inrättad svensk statlig
myndighet, som från 1 januari 2009 har ersatts av Myndigheten för
samhällskydd och beredskap. MSB är en expertmyndighet på bland annat
brandskydd
https://www.msb.se/
Brandfarliga och explosiva varor på laboratorium
https://www.msb.se/Upload/Forebyggande/brandfarlig_explosiv/SÄI Info/BEX-INFO
Informationsblad nr 1 2005.pdf
Definition av brandfarliga varor MSBFS 2010:4
https://www.msb.se/externdata/rs/84efa9ee-324a-4ebc-913a753b06e4bf0d.pdf
Myndigheten för samhällsskydd och beredskaps föreskrifter om
förvaring av explosiva varor MSBFS 2010:5
https://www.msb.se/externdata/rs/2cd29e46-fd2f-4cf3-ae7195933444982d.pdf
Sprängämnesinspektionens föreskrifter (SÄIFS 1995:3) om tillstånd
till hantering av brandfarliga gaser och vätskor med ändringar i
SÄIFS 1997:3
Den som hanterar brandfarliga gaser eller vätskor i större
mängder än som anges i tabell A skall ha tillstånd enligt lagen om brandfarliga
och explosiva varor. Kontrollrutiner ska finnas för gasolflaskor i skola, se bilaga E
https://www.msb.se/externdata/rs/721843b8-ca8d-4020-b67054ad1d3b2d95.pdf
Sprängämnesinspektionens föreskrifter (SÄIFS 1996:4) om hantering
av organiska peroxider
https://www.msb.se/externdata/rs/5f5d2d20-4307-47ed-8ad4504a046b3c88.pdf
Sprängämnesinspektionens föreskrifter (SÄIFS 1998:7) om
brandfarlig gas i lös behållare med ändringar i SÄIFS 2000:3
https://www.msb.se/externdata/rs/39b1785a-b858-4263-9e2c5dd49196dda3.pdf
Sprängämnesinspektionens föreskrifter (SÄIFS 2000:2) om
hantering av brandfarliga vätskor med ändringar i SÄIFS 2000:5
https://www.msb.se/externdata/rs/5684299c-e44e-473d-b5bdfe6a20f317a0.pdf
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Statens räddningsverks allmänna råd och kommentarer om
systematiskt brandskyddsarbete SRVFS 2004:3
Checklista över vanliga tekniska brandskyddsåtgärder samt över organisatoriska
brandskyddsåtgärder
https://www.msb.se/externdata/rs/51dc9127-8bb3-4bee-860698f694a4a5b6.pdf
Statens räddningsverks föreskrifter om explosionsfarlig miljö vid
hantering av brandfarliga gaser och vätskor SRVFS 2004:7
https://www.msb.se/externdata/rs/5a42742e-7310-4a17-aea27ca7bc821161.pdf
Definitioner samt indelning av brandfarliga vätskor i klasser SRVFS
2005:10
https://www.msb.se/externdata/rs/6ee69259-94be-4194-beb9519a4714e32f.pdf
Myndigheten för samhällsskydd och beredskaps föreskrifter om
transport av farligt gods på väg och i terräng MSBFS 2012:6 (ADR-S 2013)
https://www.msb.se/externdata/rs/50987fdb-f7da-43e9-bd3919bea9f15504.pdf
SAMIR
Säkerhet - Arbetsmiljö - Miljö – Inrapportering
(Safety-Work environment-Environment-Reporting)
(Man behöver ej logga in om man skall göra en anmälan)
https://secure.port.se/alphaquest/app_su/pcmain.cfm?verkid="%23Z%24 Vill du rapportera miljöavvikelser eller ge förslag på förbättringar inom
universitetets miljöarbete?
Gå in i det webbaserade systemet SAMIR och fyll i ett formulär. Där kan
du också anmäla tillbud, arbetsskada och brott. Du får svar via e-post på
din anmälan.
Avfall och avfallsrutiner:
http://www.su.se/medarbetare/personal/miljoarbete/miljoradet/avfall-ochavfallsrutiner-1.12153
Avfallsrutiner för Stockholms Universitet (REKOMMENDERAS!)
http://www.su.se/polopoly_fs/1.96199.1343374981!/menu/standard/file/avfallsrutin_1
20726.pdf
Waste management procedures for Stockholm University
(RECOMMENDED!)
http://www.su.se/polopoly_fs/1.100054.1347006074!/menu/standard/file/waste_mana
gement_procedures_120726.pdf
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2013
Rutiner för utsläpp av flytande kemikalierester i avlopp:
http://www.su.se/polopoly_fs/1.100051.1347005506!/menu/standard/file/rutiner_kem
ikalieavlopp_rev3.pdf
Antibiotika klassificering/sortering:
http://www.su.se/medarbetare/personal/miljoarbete/miljoradet/antibiotika1.91280
Rekommendationer för behandling av antibiotikaavfall vid Karolinska
Institutet:
http://ki.se/content/1/c6/09/18/79/Regler_antibiotika_090407.pdf
Stockholms vatten AB råd och riktlinjer för laboratorier
http://www.notisum.se/rn/nLawList.aspx?listid=26568&prefix=4e357a37
KLARA (Inventory system for chemical products)
https://secure.port.se/alphaquest/app_su/pcmain.cfm
KLARAs main focus is to present information to users about chemical
hazards and to gather information about where chemicals are being used.
Additional to this you can also perform risk assessments in the system.
You can also use KLARA to search for chemicals at the department.
Example: Ask your local KLARA person
Logon for everyone at MBW:
Username: mbw
Password:
Click on the English flag if you need an English version of KLARA and then
“Chemicals”.
From this window you may reach the chemical database, click on “Chemical
Products”. In this database you are able to find safety information (MSDS)
about aprox. 31 000 different chemical products. In order to get back to
the main menu, use the function “Back” in the left upper corner.
There is a separate search function for chemicals at our department.
Search by selecting “Inventories” from the main menu and then “Search
products”. Select group or in this case click on "Sök här" for "Inst. för
molekylär biovetenskap, Wenner-Grens institut" With this function you are able to
find the storage and amount for each registered chemical at your department.
To search for a product, it is most convenient to use the CAS number for
the product. The CAS number you will for instance find on Sigma's website:
http://www.sigmaaldrich.com
Swedish Chemicals Inspectorate (Kemikalieinspektionen)
http://www.kemi.se/
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Kemikalieinspektionens föreskrifter om klassificering och märkning av
kemiska produkter KIFS 2005:7
http://www.kemi.se/Documents/Forfattningar/KIFS/K05_7.pdf
PRIO-databasen Prioriteringsguiden verktyg att användas vid tänkt
användning av nya kemikalier eller för hjälp vid substitution:
http://www2.kemi.se/templates/PRIOframes____4045.aspx
LISTS OF RISK AND SAFETY PHRASES:
Enkla skyddsfraser / Single safety advice phrases:
http://www.kemi.se/Documents/Forfattningar/CLP/skyddsfraser.pdf
Enkla riskfraser /Single risk phrases:
http://www.kemi.se/Documents/Forfattningar/CLP/riskfraser.pdf
OM NÅGOT HÄNDER (IN CASE OF EMERGENCY)
När/om något händer - vanliga frågor
http://www.su.se/medarbetare/service/krishantering/sakerhet/nar-om-nagothander-vanliga-fragor-1.3027
Om något händer
http://www.su.se/polopoly_fs/1.104075.1349962546!/menu/standard/file/Om%
20n%C3%A5got%20h%C3%A4nder%20ny%202012%20%28Sve%29.pdf
In Case of Emergency
http://www.su.se/polopoly_fs/1.2404.1355758589!/In%20Case%20of%20Emergency
%20121106.pd
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XI.
INFORMATION PÅ SVENSKA OM GIFTER OCH
OLYCKOR
Information om olycksfall
Information och säkerhetsdatablad för de registrerade kemikalier som institutionen
innehar finns i (SUs on-line) kemikalieregister-systemet KLARA (till vilket
representanter från alla grupper inom MBW har tillträde). Hur man använder KLARA se
info under LEGISLATION AND LINKS
Alla anställda (inkl studenter) är skyldiga att anmäla alla olyckstillbud som sker på
institutionen till SU-systemet SAMIR- Säkerhet - Arbetsmiljö - Miljö – Inrapportering
(Safety-Work environment-Environment-Reporting)
https://secure.port.se/alphaquest/app_su/pcmain.cfm?verkid=%22%23Z%24%20%0A
och till xxx.
Även situationer där olycksfall skulle kunnat ske anmäls för att åtgärd skall kunna vidtas.
Försäkra er om att alla på labbet vet var nödduschar, ögonduschar och brandsläckare
finns placerade och hur dessa används.
Trasig utrustning etc som endast kräver enklare reparationer anmäls direkt till yyy.
VIKTIGA TELEFONNUMMER:
Vid nödsituation, ring 112
Vid icke-akuta förgiftningar ring Giftinformationscentralen 08-331 231 (kontorstid)
Danderyds sjukhus 08-123 550 00
Se också FÖRSTA HJÄLPEN OCH KRISSTÖD VID MBW (personer vid MBW som
har sjukvårdsutbildning) under LEGISLATION AND LINKS.
RÅD VID SPECIFIKA OLYCKSFALL
Ögonskada
Alla kemikalier skall betraktas som skadliga varför sköljning med vatten alltid ska ske
efter stänk i ögonen. Använd ögonsköljen på lab.
Vid ögonsköljning måste ögat vara öppet! Pressa isär ögonlocken med fingrarna om det
behövs. Om möjligt, avlägsna ev kontaktlinser men var observant på att frätande ämnen
kan smälta ner linsen!
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Lut eller syra (ej fluorvätesyra)
Spola omedelbart med mycket vatten och öppet öga under 15 minuter.
Kontakta läkare.
Fluorvätesyra, brom
Skölj omedelbart med mycket vatten och öppet öga i minst 15 minuter.
Uppsök sjukhus omedelbart (akutintaget) under det att ögat om möjligt ytterligare sköljs
med vatten med hjälp av sköljflaska (St Eriks sjukhus är ögonsjukhus).
Ögonakuten
Ring alltid före besök för att få medicinska råd och information om vart du ska vända dig.
Telefonnumret är 08-672 31 00 och rådgivningen är öppen dygnet runt.
Fenol
Skölj ögonblickligen med vatten och fortsätt med detta tills läkaren tar över.
Bländning
(t ex vid explosion eller UV-strålning)
Täck över ögonen.
Uppsök sjukhus (akutintaget).
Hudskada
Alla kemikalier skall betraktas som skadliga varför sköljning med vatten alltid skall ske.
Lut eller syra (ej fluorvätesyra)
Skölj med mycket vatten under det att kontaminerade kläder avlägsnas.
Kontakta läkare.
Fluorvätesyra
Skölj snabbt med mycket vatten och avlägsna kontaminerade klädesplagg.
Smörj in området som utsatts med 2% kalciumglukonat gel och fortsätt med att gnida in
tills smärtan avtar eller läkare tar över.
Uppsök sjukhus snarast (akutintaget).
Brom
Skölj snabbt med mycket vatten och avlägsna kontaminerade klädesplagg.
Tvätta noga med 5% natriumtiosulfat.
Vid svårare skada uppsök sjukhus snarast (akutintaget).
Vätecyanid
Om den skadade andas ge syrgas. Avlägsna alla kläder och skor. Om den skadade inte
andas, ge konstgjord andning med hjälp av andningsmask då annars fara för den
hjälpande uppstår.
Skölj utsatta hudområden noggrant med vatten, ev. Natriumtiosulfatlösning.
Omedelbar transport till sjukhus (akutintaget). Förvarna sjukhuset.
Fenol
Skölj omedelbart med mycket vatten. Tag av kontaminerade skor och kläder och skölj
under. Gnid så fort som möjligt och upprepade gånger in de hudområden som exponerats
med polyetylenglykol 400 eller glycerol.
Uppsök sjukhus.
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Akrylamid
Skölj omedelbart med vatten, tvätta därefter med tvål och vatten. Avlägsna
kontaminerade kläder och skölj under.
Kontakta läkare vid större olyckor.
Brännskada
Lindriga brännskador spolas med rinnande kallt vatten till smärtan avtar. Stick ej hål på
brännblåsor.
Svårare brännskador täcks med sterila kompresser, varefter den skadade transporteras
till sjukhus (akutintaget). Risk för chock föreligger (se chock).
Inandning av giftiga gaser eller kemikalier
Observera att vissa gaser och kemikalier har fördröjd skadeverkan! En person som har
inandats giftiga gaser bör hållas under uppsikt även om denne inte uppvisar några
symptom. I händelse av olycka börja alltid med att avlägsna den skadade från farozonen.
Väteklorid (HCl), Vätesulfid (H2S), Klor (Cl2), Kväveoxid (NO2), Svaveloxid (SO2)
Ge syre och konstgjord andning efter behov.
Se till att den skadade hålls varm och i vila i halvt liggande ställning även om den
skadade för ögonblicket är symptomfri.
Alla som har eller har haft symptom skall till sjukhus.
Vätefluorid (HF)
Få den skadade att snyta sig ordentligt.Se till att den skadade hålls varm och i vila i halvt
liggande ställning även om den skadade för ögonblicket är symptomfri. Kontakta läkare.
Brom (Br2)
Se till att den skadade hålls varm och i vila i halvt liggande ställning även om den
skadade för ögonblicket är symptomfri.
Alla som har eller har haft symptom skall till sjukhus.
Koloxid (CO)
Ge syre eller ev. konstgjord andning.
Kontakta genast läkare eller omedelbar transport till sjukhus.
Cyanid
Om den skadade andas ge syrgas. Avlägsna alla kläder och skor. Andas den skadade ej ge
konstgjord andning med hjälp av andningsmask då annars fara för den hjälpande uppstår.
Omedelbar transport till sjukhus (akutintaget). Förvarna sjukhuset.
Fenol
Håll den skadade varm och i vila
Kontakta läkare.
Akrylamid
Se till att den skadade hålls varm och i vila.
Kontakta läkare.
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Svalda gifter
Alla kemikalier skall betraktas som gifter, varför Giftinformationscentralen (112) alltid
ska kontaktas efter förtäring av dessa. Vid icke-akuta situationer, ring 08-331 231
(kontorstid) för giftinformation, se också http://www.giftinformation.se/
Cyanid
Om den skadade kan andas själv ge syrgas om detta finns tillgängligt. Avlägsna alla
kläder och skor. Om den skadade ej andas, ge konstgjord andning med hjälp av
andningsmask då annars fara för den hjälpande uppstår.
Om den skadade är vid fullt medvetande och helt opåverkad framkalla kräkning
omedelbart.
Omedelbar transport till sjukhus (akutintaget). Förvarna sjukhuset.
Lut, syra och ammoniak
Framkalla ej kräkning
Ge ögonblickligen mjölk eller vatten (ca 500 ml) att dricka.
Kontakta läkare.
Tungmetallsalter
(t ex salter av arsenik, barium, bly, kvicksilver)
Framkalla kräkning omedelbart. Skölj munhålan med vatten.
Omedelbar transport till sjukhus.
Fenol
Ge ögonblickligen vatten att dricka eller helst ett par matskedar matolja så fort som
möjligt.
Kontakta läkare.
Akrylamid
Är personen vid fullt medvetande skölj omedelbart munnen med vatten och ge ett glas
vatten att dricka.
Transport till sjukhus.
Skador orsakade av elspänning
Elektrisk skada
Bryt spänningen omedelbart genom att slå av huvudströmbrytaren eller ev genom
kortslutning.
Vila och värme. Vid behov ge konstgjord andning. Behandla ev. brännskador (se avsnitt
om brännskador).
Transport till sjukhus i svårare fall (akutintaget).
Chock
Chock kan uppkomma på olika sätt, t ex genom bränn- eller frätskada, förgiftning och
större blödning. Tillståndet är allvarligt och innebär en kraftig påverkan av
blodcirkulationen.
Symptom
Blekhet, kallsvett i pannan och på handflatorna. Köldfrossa och illamående.
Åtgärd
Placera den skadade med fötterna högt i ett dragfritt och varmt rum.
Håll andningsvägarna fria genom att placera den skadade i framstupa sidoläge.
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Håll den skadade varm med filtar eller dylikt. Ge aldrig någon dryck.
Kalla omedelbart på ambulans.
Lämna ej den skadade ensam, tala lugnande.
Framkallning av kräkning
Kräkning får aldrig framkallas på medvetslös person eller person som uppvisar slöhet
eller annan påverkan på allmäntillståndet då detta kan orsaka kvävning. Kräkning får
eller aldrig framkallas om syra, lut eller petroleumprodukt förtärts.
Kräkning framkallas genom att först ge den förgiftade ett glas mjölk eller vatten. Stoppa
därefter ner två fingrar i halsen på personen och håll dem kvar där tills kräkningen
kommit igång. Sluta ej även om den förgiftade börjar hulka.
DIN NÄRMASTE HJÄRTSTARTARE FINNS I STORA ENTRÉN HUS E (20C)
Andra hjärtstartare på Campus Frescati finner du på denna länk:
http://www.su.se/polopoly_fs/1.116727.1357127459!/menu/standard/file/Karta%20Campus%
20Frescati.pdf
När man tar ut hjärtstartaren ur skåpet går ett larm till väktaren samt medarbetare på
Sektionen för Säkerhet. När ni påbörjat D-HLR larma 112 samt skicka ut en person som
möter upp ambulans.
Vad är en hjärtstartare?
Hjärtstartaren har funnits inom sjukvården under lång tid. De senaste åren har det kommit
små användarvänliga apparater för icke sjukvårdsutbildade personer. Hjärtstartaren ger
talande instruktioner till dig, på svenska, som hjälpare för att utföra hjärt- lungräddning,
HLR. Hjärtstartaren analyserar sedan patientens hjärtrytm och rekommenderar om elstöt
ska ges eller ej. Hjärtstartaren är intelligent och det går inte att skjuta ström i någon som
inte behöver det.
Vem kan använda hjärtstartaren?
Hjärtstartaren kan vem som helst använda, i väskan finns en tydlig instruktionsbok hur
man ska göra samt maskinen "pratar" och ger instruktioner. Det finns inga krav idag på
utbildning. Dock bör man kunna hjärt- och lungräddning samt utbilda sig på
hjärtstartaren, det tar bara några timmar.
Utbildning För den som önskar finns det en utbildning i hur man använder hjärtstartaren,
utbildningen tar 4 timmar och kostar 375 kr/person. Anmälan görs till,[email protected]
08-16 35 45.
RAPPORTERA ALLA OLYCKOR OCH INCIDENTER VIA SAMIR
SAMIR- Säkerhet - Arbetsmiljö - Miljö – Inrapportering
Systemet har nedanstående webaddress och det bra att skriva en anmälan utan
att logga in. Du får svar på din anmälan via e-post.
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2013
https://secure.port.se/alphaquest/app_su/pcmain.cfm?verkid="%23Z%24 Brott rapporteras också till SAMIR
SAMIR kan även användas för att rapportera miljöavvikelser eller ge förslag på
förbättringar inom universitetets miljöarbete?
46
2013
MBW 2013
Everyone doing laboratory work at the Department must read the
regulations and instructions on ”Laboratory safety and management” in full.
Before starting your work, sign this paper, thereby verifying that you know the
rules and instructions understand them and will work accordingly.
Date:
Name:
Signature:
____________________________
____________________________
I hereby certify that ________________________ has read and understood the
regulations that apply to MBW lab work.
Name of mentor:
_______________________
Signature:
_______________________
Hand in the signed document to the Secretariat
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