Getting
The Most Out Of Butyrac 200 (2,4-DB Amine)
And Buctril (Bromoxynill Applications
Bill
B.
Fischer*
Abstract:
Butyrac 200 (2,4-DB amine) with adjuvants provided as effective control of certain
broadleaved weeds as was obtained with Butoxone (2,4-DB ester). Buctril (bromoxynil) has
been repeatedly demonstrated to be effective for the selective control of many broadleaved
weeds in newly planted alfalfa. The importance of proper timing of the application of these
herbicides relative to the growth of the weeds and the alfalfa cannot be over emphasized.
Their timely use can enhance weed control in alfalfa.
Introduction:
It is doubtful whether you could find another person who conducted as many applied
research trials during the past 30 years as I have in evaluating 2,4-DB and bromoxynil for
selective weed control in alfalfa.
Neither of these compounds are new and it is a sad commentary on the pesticide approval
and labeling procedures in the United States that it required thirty years to obtain a label for
the use of bromoxynil in alfalfa. We knew a quarter century ago how to get the most from
the application of Buctril in newly planted alfalfa.
Bromoxynil was first synthesized in Germany in 1896 and was patented in the United States
in 1963. Bromoxynil is an inhibitor of photosynthesis. It moves in the plant to a very
limited extent, therefore it is called a contact herbicide. At present it is classified as a
restricted use pesticide hence it can be applied only by certified applicators or persons under
their supervision.
Twenty-Five Years of Trials
The past 25 years I have conducted many trials in the San Joaquin Valley evaluating Buctril
on many different varieties of newly planted alfalfa. The trials were conducted in fall,
winter and spring planted fields on different soil types and methods of irrigation.
The label on Buctril indicates that it should be applied when the alfalfa has a minimum of
four trifoliate leaves. I have applied Buctril on alfalfa having 1 to 2 trifoliate leaves and the
only time I observed injury was when the temperature exceeded 75 to 80°F at the time of
treatment or 2 to 3 days after treatment. The symptoms observed were chlorosis or necrosis
of leaflets and some growth retardation, however these symptoms were short lived.
Rather than bore you with the results obtained in trials conducted years ago and reported at
numerous alfalfa symposia, other meetings, and in Runcina, let me briefly outline how to
get the most from the application of Buctril.
~Fann Advisor Emeritus, U.C. Cooperative Extension, Fresno County
-92-
Proper weed identification is essential. Buctril does not control grasses neither does it
control the following commonly ocurring broadleaved weeds:
chickweed
purslane
clovers .
red maids
henbit
nettle
f
There are a number of broadleaved weeds that are effectively controlled with Buctril when
seedlings however, many become more tolerant to the herbicide as they develop beyond
their seedling stage; a few of the more commonly ocurring ones are filaree, common
knotweed, cud weed and cheeseweed. Therefore proper timing of its application is essential.
In our studies we also found that more effective control was obtained with Buctril when it
was applied in 60 to 70 gallons of water per acre -than was observed when lower gallonage
was used. In fact we have been able to obtain more effective control when injected into
sprinkler irrigation systems; it is labeled for this type of application.
In Summary,
To get the most from the application
of Buctril:
a.
know the weed popula tion
h
treat weeds in their seedling stage
apply when the alfalfa has 3 to 4 trifoliate
leaves
d.
apply in 50 to 60 gallons of water per acre, this is especially important
when the weeds are beyond their seedling stage
e.
don't apply when the temperature is 80°F or above
f.
inject into irrigation system if proper conditions can be met
(
In recent years we have been evaluating the effectiveness of Buctril in combination with
other herbicides to obtain a broader spectrum of weed control. However, at present I better
turn to discuss the second part of my original topic.
Getting the Most Out Of 2,4-DB Amine
As bromoxynil, 2,4-DB amine is not a new herbicide. My first evaluation of different
formulations of 2,4-DB for the selective control of weeds in alfalfa dates back to the early
60'5.
2,4-DB was discovered in 1942 as a foliar absorbed synthetic growth regulator. It is readily
translocated in plants principally to meristematic areas of high metobolic activity where it
interferes with nucleic acid formation, disruption of translocation, metabolism and
photosynthesis. There are numerous salts and formulations of 2,4-DB and Butyrac 200 is an
amine salt of 2,4 dichlorophenoxybutyric acid.
2,4-DB is not an effective herbicide but in certain plants it is converted to 2,4-D acid through
beta oxydation. In alfalfa and clovers this does not occur as effectively as in other broadleaf
plants, hence the selectivity.
-93-
l
"
During the early 60'5 we learned that the ester formulation of 2,4-DB provided significantly
better control at lower rates than was obtained with the amine formulation. Consequently
2.4-DB ester was used by alfalfa growers until 1990 when its manufacturer discontinued its
marketing.
2,4-DB Amine vs 2,4-DB Ester
Trials were initiated during 1990 to reevaluate the amine formulation of 2,4-DB in
combination with different adjuvants and other herbicides to study whether its
effectiveness could be enhanced. These studies were summarized and published in
Runcina V 01. 45.
2,4-DB amine plus adjuvants were applied on alfalfa at different stages of its growth. It was
also evaluated in combination with Buctril, and several grass herbicides. The results of the
studies and observations can be briefly summarized as follows:
2,4-DB amine (Butyrac 200) with an adjuvant provided as effective control of
broadleaved weeds as was obtained with the 2,4-DB ester formulation (see tables).
.
The addition of X-77 at 1/4 and 1/2% of the spray volume or a paraffin based adjuvant
at 1.0 quart per acre resulted in equally as effective control of the susceptibleweOOs
with Butyrac 200 as was obtained with 2,4-DB ester.
Bor~'forrnurations
of 2,4-DB (ester...and,amincl,-,ca.used tempora~"retardation.,in.,the.
growt~{vigt)F)o.orthe"alfa.t~chowever
this was short lived.
2,4-DB amine with X-77 or a paraffin based adjuvant caused chlorosis and necrosis of
some leaflets.
The alfalfa seedlings exhibited greater tolerance to 2,4-0B amine when treated in the
first trifoliate leaf than in later stages of its growth. See the following tables.
.
When applied in combination with Poast (sethoxydim) or Select (clethodym) plus a
paraffin based adjuvant good broadleaf and grass control was obtained with excellent
alfalfa tolerance.
The younger the weeds when treated the more effective control was obtained.
Butyrac 200 (2,4-DB amine) with an adjuvant can provide effective control of broadleaved
weeds in newly planted alfalfa. Timing of its application relative to the weed growth is
critical. Many species of weeds effectively controlled in their seedling stage became more
tolerant to the herbicide as they grow. Cheeseweed, chickweed, cud weed and several other
species of broadleaved weeds are not controlled by 2,4-DB as shown in chart 1.
In Summary
Butyrac 200 plus an adjuvant and Buctril are effective herbicides for the selective control of
broadleaved weeds. Alfalfa growers can obtain the most from their application only
through correct identification of the weed population and proper timing of their application
relative to the growth of the weeds and the alfalfa. If the conditions outlined above are met
these herbicides can be effective tools in the arsenal of alfalfa growers for the control of
unwanted competing vegetation in their fields.
-94-
*
NOT
FOR
crSB
Susceptibility
LABELED
IN
CHART 1
of Weeds to Herbicides Evaluated
-95-
in Alfalfa Production
f
--
Summary of Herbicide
Performance
Method of
ApplIcation
PREPLANT
INCORPORATED
CHART 2
Evaluated in Alfalfa Production
Conditions Th.t R.sult8d
Most Effectlv. Control
H8rtIlcjde
In
in the Central San Joaquin Valley
Conditions That Resulted In Poor
Poor C~:1trol or b1jury
Balan~
(beneffn)
Shallow incorporated in well prepa'U.seed bed. When »mperature was
favora~e for rapid seeding growU1.
Only suscepti~
weeds were present
Cool .~ture.
deep i~rpora1ion
volun-r
cereals and ottler resistant
~
species were ~esenl
Eptam~
Thorough
i~rporation
in spring planted fields.
Win8r application, daddy seed OOd
aM resistant weeds present. Where
longer tt1an 6 weeks of residual
control -.s needed.
(EPrC)
rXJtsedge
-ks
Butyrac 200
(2,4-08 arrine +
was present;
early fag or
Wl'-.re
wtlere
4 to 8
conb"ol was needed.
WMn broadeaf weeds in their seeding
Slage were the only weeds present
Broadeal weeds beyoOO tt1eir
seedling stage ~re IX'8senl
KQ(b<!)
(pronamide)
When alfalfa had two trifoliate leaves
a 00 weeds were not emerged or in
their seedling stage; rainfall or
SfX'inkler irrigation was appied
following trearnent.
When alfalfa was in the seedleaf or
unfoliate leaf stage of growth severe
injury occurred. When -005
-re
beyoOO tt1eir seedling stage. When
water was not applied soon after
treatment.
Buclril~..
(bromoxynil)
When the alfalfa had 2 to 3 bifoliate
leaves and 1fIe broadleaf weeds
were in their seedling stage. Applied
in 60 gallons of water per acre and
tt1e alfalfa and broadleat weeds were
When grasses, chickweed and
redmaids were rxevalent. Weeds
were beyond 1h!ir seedling stage
and air a~icalion
in 10 gpa was
used. When U1e temperawre was
above 80°F temporary yellowing of
It1e alfalfa was observed.
a~uvant)
POSTEMERGENCE
growing vigorously.
Poast~
(sethoxydim)
When grasses are in their seedling
stage and growing vigorously.
Velpar~
(hexazinone)
Alfatfa well established with a well
developed root system 6. long or
or longer with a top growth that is
6. to 8., Most effective on weeds in
their seedling stage. Sprinkler
irrigation or rainfall shortly after
appication is desira~
lor ITKJst
effective control.
Eptam~
(EPTC)
Ap~ied in tt1e irrigaDon water and
only when ~rough
wetting of ~
bed top was obtained. Timely
repeated ap~ication was made.
Grasses beyom their seedling stage
am moisture stressed.
Alfalfa seedlings oot having
6. long roots. Weeds predominantly
graS5es and broadleaf -ds
5. to 6.
or older. No rainfall or irrigation
following 'realmenl
Poor water
wetting
a~ication
distribuuon
the bed top.
and not
Re~ated
was not made.
Kar~x~
(diuron)
When applied on soil free of clods and
volunteer alfalfa seed germination.
A(Xjition of paraQuat improved weed control
when groundseVwas present.
Applied on trashy or cloddy soil.
Applied after Feb. 1st on nondormant varieties resulted in injury
Treflan~
(Irinuralin)
Thoroughly irx:orporated with rolling
cultivators in fields tree ot established
'\Weds. Sum~r
annual grasses,
pigweeds am lambsquarter, the
only -ds
rxesenl
Not irw:orporated thoroughly
or when resistant weed species
predominated.
Treflan TR-10
(trifluralin 10%
Unifom1ly distributed by mid Feb'uary before
before dodder seed gem1ination. Rainfall
occurs or irrigation applied within 4 to 6 days
following application. Will provide effective
preernerge~
grass control.
Ap~ied after dodder is attached. Rainfall
does not occur or field not irrigated within
a week following ap~ication.
Alfalfa was established
longer .Ap~ied
during
Established grasses were ~
predomnant -ds.
Broadleat
weeds 8 10 10 inches taIlor taller.
ApPied at1Br the middle ot February
ESTABUSHED
STANDS
granular)
Velpar~
(hexazinoM)
and January
one year or
December
'M1en ItMi alfalfa
growth
was soon (4 to 6 inches).
Rainfall
occurred
shortly after ap~ication
and weeds were 2 to 4 irK:hes tall.
.This is not a recommendation for the use of herbicides.
It is a progress report of obselVaDons made in applied research trials conducted
in Fresno County.
..As of December 1990. registered by EPA but not labeled for use in California.
-96-
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Table 4- Effect of Postemergence Applied
on Weed Control in Alfalfa
02,43,425,94,10,91-2
LOCA naN :
SOIL TYPE:
V ARIETY:
TREATED:
Keamey Agricultural
Center
Hanford sand y loam
Hay mix
2/23/91; Alfalfa,4 to 7 trifol. lvs.
.Weeds
in bud stage
Yield
Lbai/
Acre
Heroiddes
Total
Yield
in Grams
Herbicides
IRRIGAnON:
PLOT SIZE:
PLANTED:
EV ALUATED:
Flood,1/31/91
10' x 20', Reps 4
10/31/90
5/24/91
Per 4 ft ~ Area. Avg: of 4 Re12lications
Cudweed
Pineapple~
Shepherd's
~
Other
Weeds
1.0
1/2%
753
719
21
11
1
X-77
B
2,4-DB amine
X-77
1.0
1/4%
744
708
26
9
1
0
c
2,4-DB amine
X-77
1.5
1/4%
656
629
18
7
2
0
o
2,4- OB
1.5
1/2%
698
664
22
10
1
X-77
Pursuit
Buctril
X-77
0.125
0.33
1/2%
726
681
34
8
1
1
F
Buctril
0.33
705
690
1
s
0
9
G
Pursuit
0.125
1.0
1/2%
692
683
0
3
0
6
704
643
49
s
0
7
A
F
2,4-DB
2,4-DB
amine
amine
amine
X-77
H
Untreated
Statistical
Notation:
..Other Weeds:
LSD @5%
C.V.
fiddleneck,
groundsel,
NS
17.95
86.74%
horseweed, sowthistle
-100-
NS
NS
4.54
142.8%
I
f
Table 5- Effect of Postemergence Applied Herbicides
on Vigor, Injury and Weed Control in Alfalfa
02, 43, 425, 94, 10, 91-2
Keamey Agricultural Center
Hanford sandy loam
Hay mix
2/23/91: Alfalfa 4 to 7 trifol. lvs.
Weeds in bud to early flower stage
LOCATION:
SOIL TYPE:
VARIETY:
TREATED:
Vigor,
Injury
and
IRRIGA TION :
PLOT SIZE:
PLANTED:
EVALUATED:
Weed
Control
Flood
10' x 20', Reps
4
10/31/90
See below
Evaluationsf
Avg.
4 Re!2s.
91
Lbai/
Herbicides
Acre
Weed
Vi
Control
fa
Vi
1.0
1/2%
8.5
2.5
39
9.5
1.8
0
0
0
X-77
B
2,4-DB amine
X-77
1.0
1/4%
9.0
1.8
49
10.0
0.5
25
0
0
c
2,4-DB
1.5
1/4%
8.3
2.3
46
9.3
1.8
19
6
0
7.8
2.5
44
9.3
1.5
39
31
19
A
2,4-DB
amine
c
amine
X-77
D
2,4-DB
amine
1.5
X-77
1/2%
Pursuit
Buctril
X-77
0.125
0.33
1/2%
7.5
2.8
69
8.0
2.3
49
13
19
F
Buctril
0.33
8.5
0.8
81
9.3
1.8
99
18
13
G
Pursuit
2,4-DB amine
X-77
0.125
1.0
1/2%
8.3
0.5
78
9.0
0.5
94
56
46
10.0
0
0
10.0
0
0
0
0
E
H
Untreated
Statistical Notation:
LSD @5%
C. V.
0.60
0.67
6.89% 39.72%
11.4
0.64
21.81% 6.71%
0.54
41.86%
35.68
84.6%
9.8
(
9.8
9.8
23.51
15.88
146.16% 126.92%
.Vigor rating based on a scale of 0 to 10; 0 = dead plants, 10 = vigorously growing plants.
Injury rating based on a scale of 0 to 10; 0 = no injury; 10 = severe injury or death.
Weed
abbreviations:
Sp = shepherd's
purse,
y = pineappleweed,
R = redmaids.
,
t
"",~
-101-
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