GC CARE
Gas Chromatography Columns
Innovation with Integrity
Gas Chromatography
Bruker’s commitment to providing
the very best in analytical systems
extends beyond providing great
instrumentation. In most cases, a
good analytical system encompasses
instruments, software, and high value
consumables. Bruker applies the same
demanding standards of performance,
utility, and readily available expert
support to all components of our
analytical systems.
A Selection of GC Columns
to Meet Your Needs
Bruker GC columns span a broad range
of column diameters, stationary phases,
and capillary column materials: Fused
Silica (FS) and Inert Steel (IS). Ideal for
either routine or research type analyses.
Bruker GC column offerings bridge
across many important applications and
include a number of offerings such as:
■ Standard WCOT (Wall Coated Open
Tubular) Columns with an internal diameter ranging from 0.25 mm to 0.53
mm in a variety of stationary phases
(from the non-polar BR-1 and BR-1ms
phase up to the wax phases) for
narrow and wide analyte concentration
ranges.
■ Small Internal Diameter Columns for
fast analysis that improve cycle time
while maintaining high resolution.
■ Solid Stationary Phase PLOT (Porous
Layer Open Tubular) Columns for the
analysis of volatiles that require high
selectivity. The solid stationary phase
is tuned for applications commonly
used for the permanent gas analysis
like the BR-Molsieve 5A and petrochemical analysis like the BR-Alumina
range.
■ Inert Steel Micro-Packed and Packed
Columns for composition analysis
with bulk components, where a high
column capacity is required.
Petrochemical and
Hydrocarbon Analysis
Bruker provides the most comprehensive
range of GC analysis solutions for analysts working in the Petrochemical industry. Bruker configures, produces, tests,
delivers, and installs gas chromatography hardware, complete with columns,
software and consumables according
to widely used industry standards (e.g.
ASTM, UOP, ISO, GPA, EN). This saves
you time and ensures confidence in your
results.
Low Bleed, High Temperature
C40
C12
Directed Solutions for Distillation
Simulated distillation per ASTM D7169
is used for determining the boiling point
range distribution of petroleum products
by capillary gas chromatography of heavy
distillate and residual fuel oils with initial
boiling points (IBP) > 100°C and final
boiling point (FBP) above 720°C. The stationary phase must meet rigid resolution
and retention time requirements, yet be
stable at high temperatures.
C100
0
5
10
15
20
25
30
35
Figure 1 shows a Polywax 1000 separation on a
BR-1HT SimDist column. Chromatogram was obtained under
temperature programming up to 430 oC.
High Selectivity Separation
1
2
1. methane
2. ethane
3. ethylene
4. propane
5. propylene
6. isobutane
7. n-butane
8. propadiene
9. acetylene
10. trans-2-butene
11. 1-butene
12. isobutylene
13. cis-2-butene
14. isopentane
15. n-pentane
16. 1,3-butadiene
17. trans-2-pentene
18. 2-methyl-2-butene
19. 1-pentene
20. cis-2-pentene
21. hexanes
6
4
7
To meet the critical criteria set forth
by ASTM, Bruker is using the BR-1HT
SimDist column. The BR-1HT polymer
is a 100% polydimethylsiloxane (PDMS)
material that requires minimal conditioning, and is 100% crosslinked and coated
onto highly deactivated stainless steel
tubing that has the inertness of fused
silica without the temperature limitations.
The BR-1HT inert steel SimDist column
has a lifetime of at least 400 injections
under typical SimDist conditions.
40
3
5
10
11
16
13
12
14
15
9
17
8
19
18
0
2
4
6
8
10
12
20
21
14
Figure 2 shows excellent retention and selectivity on a
BR Alumina Na2SO 4 PLOT column for a complex refinery gas
sample; complete separation up to Hexanes within 15 minutes.
Additional Petrochemical
Applications
Apart from the inert steel SimDist columns, Bruker offers a
complete column product range for the petrochemical industry
including (micro-)packed for refinery gas analysis, PLOT columns
for selective volatiles analysis, high resolution columns for
detailed hydrocarbon analysis.
Pharmaceutical
Gas chromatography in the pharmaceutical industry has been identified as a
powerful tool to analyze volatiles in
complex samples. With today’s more
complex pharmaceuticals/biotherapeutics and sophisticated formulations and
delivery systems, the need for precise
and accurate analysis to deliver safe and
effective drugs has become extremely
urgent.
Greater Resolution
Head Space Testing and Residual
Solvent Analysis
The United States Pharmacopeia (USP)
incorporated headspace-GC analysis
into its Residual Solvents Chapter: USP
467. Although analysis of the headspace
matrix is usually straightforward, Bruker
offers the columns to separate and
analyze these components in a fast and
accurate way. Due to the nature of some
solvents like small organic amines, high
column inertness is required to generate
a symmetrical peak shape and as a result
improve identification and quantitation
especially at lower concentrations.
13/14
7
1. Methanol
1. Methanol
2. Acetonitrile
2. Acetonitrile
3. Dichloromethane
3. Dichloromethane
4. trans-1,2-Dichloroethene
4. trans-1,2-Dichloroethene
5. cis-1,2-Dichloroethene
5. cis-1,2-Dichloroethene
6. Tetrahydrofuran
6. Tetrahydrofuran
7. Cyclohexane
7. Cyclohexane
8. Methylcyclohexane
8. Methylcyclohexane
9. 1,4-Dioxane
9. 1,4-Dioxane
10. Toluene
10. Toluene
11. Chlorobenzene
11. Chlorobenzene
12. Ethylbenzene
12. Ethylbenzene
13. m-Xylene
13. m-Xylene
14. p-Xylene
14. p-Xylene
15. o-Xylene
15. o-Xylene
8
12
10
4
11
15
5
1
2
3
0
6
5
9
10
15
20
25
30
Figure 1: Improve system suitability pass rates with greater
resolution on BR-624ms columns
Higher Sensitivity
6
5
7
8
9
12
1. Methyl methanesulfonate (MMS)
2. Isopropyl methanesulfonate (iPMS)
3. Diethyl sulfate (DES)
1. Methyl methanesulfonate (MMS)
4. di-isopropyl
sulfate (DPS)
2. Isopropyl methanesulfonate
(iPMS)
3. Diethyl
sulfate (DES)
5. dibutyl
sulfate
(DBS)
4. di-isopropyl sulfate (DPS)
6. Methyl
benzenesulfonate (MBS)
5. dibutyl sulfate (DBS)
7. Ethyl
benzenesulfonate
6. Methyl
benzenesulfonate (MBS)(EBS)
7. Ethyl benzenesulfonate
(EBS)
8. Methyl
toluenesulfonate
(MTS)
8. Methyl toluenesulfonate (MTS)
9. Ethyl
toluenesulfonate
9. Ethyl
toluenesulfonate (ETS) (ETS)
10.
n-propyl
toluenesulfonate
(nPTS) (nPTS)
10. n-propyl toluenesulfonate
11. n-butyl benzenesulfonate (nBBS)
11. n-butyl
benzenesulfonate (nBBS)
12. isopropyl p-toluenesulfonate (iPTS)
12. isopropyl
p-toluenesulfonate
(iPTS)
13. p-toluenesulfonic
acid n-butyl ester (nBTS)
13. p-toluenesulfonic acid n-butyl ester (nBTS)
11
Analysis of Polar Unknowns
13
4
For confirmation analysis of polar
unknowns, a GC/MS configuration is
used. To optimize speed of analysis,
and enable an optimal selectivity while
minimizing column bleed the Bruker ‘ms’
column line offers the most versatile
solutions. For example the BR-624ms is
a medium polar and provides improved
retention and selectivity for also polar
compounds and are also more compatible with polar injection solvents.
High column inertness is essential for
polar component analysis. With the
Bruker inert columns, highly polar components like glycols, and sulfonates like
mesylate, besylate, and tosylate that are
sensitive to react with the column phase,
give the peak symmetry needed for good
quantification.
1
2
3
10
Figure 2: The BR-624ms phase is more compatible with polar
injection solvents than type BR-1 or BR-5 columns, providing higher
sensitivity and less time needed for optimizing injection parameters.
Multiple traces using MS detection.
Environmental Analysis
Governed by institutions as EPA and EN,
today’s Environmental analysis market is
more stringent and challenging than ever.
The levels of active or hazardous components to be measured are extremely
low, whilst the array of sample matrices
encountered is wide and most often
quite challenging.
Bruker Capillary Columns offer exceptional thermal stability in combination with
extremely low bleed and high inertness
levels, to give you the optimum performance for your GC or GC/MS system. All
these column features help you meet the
analysis quality requirements set forth by
various regulatory agencies.
Analyte Class:
■ Semi volatiles
■ PCB’s & Arochlors
■ Pesticides
■ Volatiles
■ Polyaromatic Hydrocarbons
Exceptional column quality minimizes
downtime and optimizes profit per
sample, while fully maintaining the integrity of your results. Bruker offers a complete package of solutions, with columns
engineered and produced precisely for
the application you need.
Optimization for the Analysis of
Volatiles and Pesticides
An example of application-based
column optimization is our solution for
EPA Method 8270 semi-volatiles. The
BR-5MS provides excellent column
inertness towards acidic, basic, and
neutral compounds found in this analyses. BR-5MS also provides excellent
resolution of high molecular weight PAHs
like benzo (b) and (k) fluoranthenea and
possess unsurpassed thermal stability
make this column the best choice for the
method.
Method EPA 8270
1. 1,4-Dioxane
2. N-Nitrosodimethylamine
3. Pyridine
51. 2,4-Dinitrophenol
87. Benzo[b]fluoranthene
88. Benzo[k]fluoranthene
Figure 1: shows illustration
of method EPA 8270 on a
BR-5ms column
Clinical and Forensic Analysis
Analysis of post-mortem tissue or biofluid
samples is challenged by producing critical evidence that stands up under severe
scrutiny. Pressure for fast & definitive
results drives labs to explore potential to
improve quality of data or speed methods
up without compromising data quality.
Exceptional Performance
1. Amphetamine
2. Methamphetamine
3. MDA
4. MDMA
5. MDEA
c = contaminant
Drug Screening and Forensic
Analysis
Bruker provides you with the best solutions in the Clinical/Forensic workplace
with columns that are manufactured
and individually tested to meet stringent
requirements for inertness, low bleed,
and unsurpassed column to column
reproducibility.
The exceptional inertness improves peak
shape over ‘normal’ 5% diphenyl-phase
columns. As a result, analytical sensitivity
for medium-polar active components
such as derivatized amphetamines or
cocaine improves significantly. This
ruggedness also ‘lasts longer’, even
after repeated injections with aggressive
derivatization reagents. Utilizing the same
column derivatized cannabinoids and a
wide range of other regular drugs may be
analyzed with similar or better results.
Figure 1: shows the analysis of derivated amphetamines. The
rugged column results in a highly stable performance on the
BR-5ms, even under the most demanding analytical conditions
and its excpetional inertness ensure good peak shapes for
reproducible quantitation.
Wide Range of Use
3
1.
2.
3.
4.
Cannabidiol
delta-9-THC
Cannabinol
THC-COOH; THCA
1
2
4
8.0
9.0
10.0
11.0
12.0 min.
Figure 2 shows the analysis of derivatized cannabinoids. Again,
the stability of the BR-5ms results in exceptional performance.
This column can be used on a wide range of other regular drugs,
such as opiates or cocaine with similar performance.
Food, Flavors and Fragrances
There is an immense range of analytes of
interest and matrices that can be encountered in the analysis of food, flavor, and
fragrances. Many governing organizations have developed requirements for
testing these important products. Recent
developments and product quality issues
have driven the need for more and more
content product labeling and reporting.
High Temperature Application
1. 5-a-cholestane (IS)
2. Cholesterol
Analysis of Fatty Acids and Oils in
Foods
For Food applications, Bruker offers a
range of gas chromatographic columns
for a number of analyses described by
organizations like the Association of
Official Analytical Chemists (AOAC) International, AOCS (American Oil Chemists
Society), AACC (American Association
of Cereal Chemists) and INA (Insti­tute for
Nutraceutical Advancement).
Bruker offers solutions for the analysis
of Fats & Oils for free fatty acids content
either directly for volatile acids or methyl
derivatized for non- and semi-volatiles
(FAMES). For triglycerides analysis
Bruker offers columns that are capable of
operate at elevated column temperature
conditions. Also for cholesterol and other
dietary Sterols column solutions are
available.
Analysis of Composition in Flavors
and Fragrances
Complex samples like flavors and fragrances require powerful analytical techniques to separate components of interest.
Besides the high separation power also,
by optimizing the polarity of the stationary phase, the Bruker GC columns have
additional selectivity that allows better
identification and quantification of many
key components of these naturally occurring and synthetic products.
Figure 1: chromatography of underivatized cholesterol, column
programmed to 330°C.
Powerful Separation
Powerful Separation
1. heptanol
2. a-thujene
3. a-pinene
4. camphene
5. sabinene
6. β-pinene
7. 6-methyl-5-hepten-2-one
8. myrcene
9. octanal
10. a-phellandrene
11. 3-carene
12. a-terpinene
13. p-cymene
14. limonene
15. a-terpinene
16. octanol
17. terpinolene
18. linalool
19. nonanal
20. citronellal
21. terpinene-4-ol
22. a-terpineol
23. decanol
24. octyl acetate
25. nerol
26. neral
27. carvone
28. geraniol
29. geranial
30. nonyl acetate
31. citronellyl acetate
32. neryl acetate
33. geranyl acetate
34. dodecanal
35. β-caryophyllene
36. trans-a-bergamotene
37. a-humulene
38. β-bisabolene
Figure 2: split injection of a neat lemon oil on the BR-5 column
A Complete Range of High
Performing Stationary Phases
Similar Stationary Phases
BR-1ms
100% dimethyl polysiloxane HP-1ms UI, DB-1ms UI, HP-1, HP-1ms,
DB-1,DB-1ms, Ultra-, 1 VF-1ms, CP-Sil 5,
CP Sil 5 CB Low Bleed/MS, Rxi-1ms, ZB-1,
ZB-1ms, BP-1, Optima-1, Optima-1ms,
SPB-1, Equity-1
BR-1HT
100% dimethyl polysiloxane
DB-1HT , Rxi-1HT , ZB-1HT
BR-5
5% diphenyl
95% dimethyl polysiloxane
HP-5ms UI, HP-5, HP-5ms, DB-5, Ultra-2,
CP-Sil 8, CP Sil 8 CB, Rxi-5ms, ZB-5, BP-5,
Optima-5, SPB-5, Equity-5
BR-5ms
5% phenyl
95% dimethyl arylene siloxane
DB-5ms UI, DB-5ms, VF-5ms, CP-Sil 8 CB
Low Bleed/MS, Rxi-5Sil MS, ZB-5MS,
BPX-5, Optima-5ms, SLB-5
BR-5HT
5% diphenyl
95% dimethyl polysiloxane
DB-5HT, VF-5HT, Rxi-5HT, ZB-5HT
BR-XLB
arylene/
methyl modified polysiloxane
DB-XLB, VF-Xms, Rxi-XLB
BR-624ms
6% cyanopropyl phenyl
94% dimethyl arylene siloxane DB-624, HP-624, VF-624ms, Rxi-624Sil MS,
ZB-624, BP-624, Optima-624
BR-35ms
35% phenyl
65% dimethyl arylene siloxane
DB-35ms, VF-35ms, Rxi-35, Sil MS, MR2
BR-17
50% diphenyl
50% dimethyl polysiloxane
HP-17, DB-17, DB-608 ,CP-Sil 24 CB, Rxi-17,
ZB-50
BR-17ms
50% phenyl
50% dimethyl arylene siloxane
DB-17ms, VF-17ms, Rxi-17Sil MS, BPX-50
BR-Alumina
Alumina Na2SO4 deactivated Na2SO4 Rt-Alumina Bond, GS-Alumina,
HP PLOT S, CP-Al2O3/Na2SO4,
Alumina-PLOT, AT-Alumina
BR-Q PLOT
Divinylbenzene polymer
RT-Q-BOND, CP-PoraPLOT Q, CP-PoraBond
Q, Supel-Q-PLOT, AT-Q
BR-QS PLOT
Porous divinylbenzene polymer
RT-QS-BOND, GS-Q
BR-S PLOT
Divinylbenzene 4-vinylpyridine
RT-S-BOND, CP-PoraPLOT S, Supel-G45
BR-U PLOT
Divinylbenzene ethylene glycol/dimethylacrylate
HP-PLOT U, RT-U-BOND, CP-PoraPLOT U,
CP-PoraBond U, Supel-N PLOT
BR-Molsieve5A
Molecular Sieve 5A
RT-Molsieve 5A, GS-Molsieve, HP PLOT
Molsieve, CP-Molsieve 5A, AT-Molsieve,
PLT-5A
Bruker Daltonik GmbH
Bruker Daltonics Inc.
Bremen · Germany
Phone +49 (421) 2205-0
Fax +49 (421) 2205-103
[email protected]
Billerica, MA · USA
Phone +1 (978) 663-3660
Fax +1 (978) 667-5993
[email protected]
www.bruker.com/chemicalanalysis
to change specifications without notice. © BDAL 02-2011. #275544
Composition
Bruker Daltonics is continually improving its products and reserves the right
Bruker
Phase