MICROBES AND THE NITROGEN CYCLE Megen Groski Ross/Woodward School, New Haven, CT ([email protected]) Ruth A. Gyure (mentor), Western CT State University ([email protected]) SAMPLE PRE AND POST TEST QUESTIONS: INTRODUCTION: This project addresses scientific method of inquiry, and the role of microbes in ecosystems (especially the nitrogen cycle. 1. What are bacteria? a. multiple-cell animals c. infections 2. What do bacteria in our soils and waterways do to help our environment? a. they serve as food for other organisms b. they decompose dead organisms and animal wastes into chemical elements c. Once ingested, they can protect animals from transmitted diseases. d. can protect plants from diseases. PROJECT GOALS: •BETTER METHODS for growing and using micro-organisms at the middle school level (some approaches work much better than others) •An emphasis on QUANTITATIVE methods of working with microbes and chemical aspects of the cycles, using counting, proportions, measurements, graphing and interpretation of graphs and number sets. •UNDERSTANDING the prevalence and diversity of microbes and their beneficial role in ecosystems and in the body. •Ability to DESIGN AND CARRY OUT EXPERIMENTS to test the role of microbes in assisting plants to obtain nitrogen from the air. b. single-cell organisms d. vitamins www.science.uwaterloo.ca/~tcharles/rhizobiu m.html •APPLICATION to real life issues such as farming, sewage treatment, industry, pollution. DISCUSSION: Students were excited about this hands on learning experience. They enjoyed collecting data and made several comments about “feeling like a scientist”. I had hoped that they would gain an understanding of the 3. What do helpful bacteria do for humans and animals? nitrogen cycle as well as learn about conducting an a. They help digest food and destroy harmful organisms in the experiment. I think that parts of the Nitrogen cycle got lost intestines along the way but they truly loved gathering data and then b. Intestinal bacteria help the body absorb vitamins interpreting it. c. They ward off warts After completing this project I feel that my students have not only met the intended goals but are also confident in completing any scientific task put in from of them. They particularly enjoyed interpreting data and explaining their thoughts to me about the how’s and why’s of the experiment. METHODS: •Students posed questions about the growth of pea plants •with and without nitrogen fertilizer, and helpful bacterial inoculum (root-colonizing Rhizobium). •Hypotheses about which plants would grow better were developed. •Experiments were set up using 3 of each type of bag and 3 to 4 seeds in each bag. •For several weeks students watched the seeds recording their observations. After a month students took measurements from each bag to determine which treatment grew the largest plant. •Students recorded their data in charts and constructed graphs to help reach their conclusions. REFERENCES •Classroom Aquaponics: Exploring Nitrogen Cycling in a Closed System Teacher’s Guide: csip.cornell.edu/Curriculum_Resources/CEIRP/Aquaponics.htm •Teaching Ecology Newsletter www.britishecologicalsociety.org/articles/education/resources •Vision Learning www.visionlearning.com •Wiggins, G. and McTighe, J. (2005). Understanding by Design, Expanded Second Edition. Alexandria, VA: Association for Supervision and Curriculum Development. •Windows to the Universe: www.windows.ucar.edu/tour/link
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