Biology 105 – Human Biology
Session:
Section:
Class Location:
Days / Time:
Instructor:
Spring 2013
55244 4 Units
UVC1 St. Helena
F 9:00 AM – 3:50 PM
RIDDELL
Student ID#: 101123456
Student Name: Biolo Gee
Team Name:
Lab Assignment #: 1
Date:
2013 02 01
Lab Title: Mitosis, Meiosis and Gametogenesis
Purpose / Objective(s):
1. Observe Cheek Cells
2. Observe Mitosis in prepared MS slides of Whitefish Blastula via standard Light Microscopy
3. Observe Human Gametes in prepared MS Slides via standard Light Microscopy
Survey and capture illustrations and photos from the internet of the above
Hypothesis:
NA
Materials / Subjects / Specimens:
Specimen of cheek cells form self
Prepared MS slides featuring a cross-section of:
 White Fish Blastudisc Cells
 Sperm Smear Human
 Ovary Maturing, Follicle Human
.
Methods / Tools / Instrumentation / Procedures:
1. Prepared a MS sample of cheek cells from self and observed via standard Light Microscopy
2. Standard Light microscope with 100, 400 and 1000 Magnification
3. View cheek cells at 100X and 400 X
4. View and locate the phases of Mitosis is Whitefish Blastudisc Cells
5. View Human sperm smear, and view ovary maturing, and follicle sections in human ovary
BIO 105 Lab 2 Mitosis, Meiosis and Gametogenesis
BGee 130201.1
Biology 105 – Human Biology
Session:
Section:
Class Location:
Days / Time:
Instructor:
Spring 2013
55244 4 Units
UVC1 St. Helena
F 9:00 AM – 3:50 PM
RIDDELL
Results
Viewing the target specimens and structures with the standard light microscopes required skill and
patience.
Photography of viewed specimens was not available.
As a resort, research, identification and selection of target specimen photos was performed via internet
search for appropriate images.
Phases of Mitosis are presented in Table 1
 Inter
 Pro
 Meta
 Ana
 Telo
 and Cytokinesis
Phases of Meiosis II are presented in Table 2
 Pro II
 Meta II
 Ana II
 TeloII
 and Cytokinesis II
Gametogenesis tissues and cells are presented in Table 3
Male and Female structures
Analysis / Discussion:
 See Table 1 and 2 (Description of mitosis events and description of meiosis events.)
 See Chart Diagram 1 for a brief Comparison of mitosis and meiosis
 The key results of mitosis are ……..
 …
 ….
 ….
 …..
Events of Mitosis
Interphase
Cell growth, prepares to be split into two cells. Everything being duplicated. G1, S, G2
stages are occurring in this stage of interphase.
Prophase
Chromatin fibers become tightly coiled and folded. Then in prometaphase the nuclear
envelope breaks into fragments and eventually disappears.
Metaphase
Chromosomes align together in the middle of the plate. Spindles together from the top to
bottom pole. Each pole has a set of centrioles
Anaphase
Chromosomes move to opposite sides of the poles, and spindles begin to pull and break
apart as well as the chromosomes
Telophase
Cleavage between the two cells are left to begin the formation of two separate cells. Half of
the DNA is taken to one part of the cell, while the other part remains in another cell. Later
forming two separate cells.
BIO 105 Lab 2 Mitosis, Meiosis and Gametogenesis
BGee 130201.1
Biology 105 – Human Biology

Session:
Section:
Class Location:
Days / Time:
Instructor:
Spring 2013
55244 4 Units
UVC1 St. Helena
F 9:00 AM – 3:50 PM
RIDDELL
The key results for meiosis are…..
 …
 ….
 ….
 …..
Events of Meiosis
Interphase
Cell growth, cell doubling DNA.
Chromosomes duplicate
Chromatin inside nuclear envelope, and centriole pair with another centriole to make
centrioles
Prophase
Centromere attaches to the spindles and the centromere begin to form. Spindles stretch
and spread apart and the centromere begin to bind to the spindles. In the process of
synapsis the two sister chromatids form and attach together
Metaphase
The chromosome tetrads are aligned on the plate. The centrioles are at opposite sides of
the pole. Spindle fibers are aligned around the entire cell.
Anaphase
Daughter chromosomes begin to split apart and leave to opposite ends of the cell; the
spindles also start to break apart. The centrioled remain at opposite ends of the poles.
Telophase
Cleavage furrow is in between the cells and the two cells are beginning to form a formation
to leave into the next stage of Cytokinesis. This is when the cell officially forms into two
cells and the two sister chromatids separate. Then the cycle repeats itself into mitosis, and
in the very end we are left with four duplicate cells.
BIO 105 Lab 2 Mitosis, Meiosis and Gametogenesis
BGee 130201.1
Biology 105 – Human Biology

Session:
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The key differences between Mitosis and Meiosis are:
 …
 ….
 ….
 …..
Figure 1: (Comparison of Mitosis and Meiosis)
Spring 2013
55244 4 Units
UVC1 St. Helena
F 9:00 AM – 3:50 PM
RIDDELL
See Figure 1.

BIO 105 Lab 2 Mitosis, Meiosis and Gametogenesis
BGee 130201.1
Biology 105 – Human Biology
Session:
Section:
Class Location:
Days / Time:
Instructor:
Spring 2013
55244 4 Units
UVC1 St. Helena
F 9:00 AM – 3:50 PM
RIDDELL
Conclusions/Further Considerations:



………
………..
………….
References
1. http://webnt.calhoun.edu/distance/internet/natural/anatomy/EndoRepro/ovary4X.html
2. http://faculty.baruch.cuny.edu/jwahlert/bio1003/mitosis.html
BIO 105 Lab 2 Mitosis, Meiosis and Gametogenesis
BGee 130201.1
Biology 105 – Human Biology
Session:
Section:
Class Location:
Days / Time:
Instructor:
Spring 2013
55244 4 Units
UVC1 St. Helena
F 9:00 AM – 3:50 PM
RIDDELL
ATTACHMENTS:
Table 1: Illustrations and Micrographs of Each Mitosis Stage
Stage
Key Event(s)
Notes /
Description / Slide
Specimen
Interphase
Chromosomes
begin to form
Cell growth
Prophase
Chromatids pair
up and make
chromosomes
Metaphase
Chromosomes
shre DNA and
line up in a line
Replicated
chromosomes,
consisting of two
sister chromatids
held together at the
centromere
chromosomes
assemble on the
equatorial plate
Anaphase
Chromosomes
split apart
Sister chromatids
separate from each
other and move to
opposite poles of the
cell.
Telophase
&
Cytoknesis
All DNA is
doubles and split
into their cells
Cells are separated
Illustration/
Diagram
Photo
Micrograph
Web Reference for white fish and Onion Root cells:
http://faculty.baruch.cuny.edu/jwahlert/bio1003/mitosis.html
BIO 105 Lab 2 Mitosis, Meiosis and Gametogenesis
BGee 130201.1
Biology 105 – Human Biology
Session:
Section:
Class Location:
Days / Time:
Instructor:
Spring 2013
55244 4 Units
UVC1 St. Helena
F 9:00 AM – 3:50 PM
RIDDELL
Table 2: Illustration and Photo Micrographs of Meiosis 1 and Meiosis 2
Stages
Meiosis
Key event(s)
Notes/Description/
slide specimen
Prophase
Phase 1
Cleavage
furrow is
present in
between both
cells
Cells uncoil and
nuclear envelope
forms
Metaphase
Phase 2
Spindles align
with each other
Chromosomes pair
up with each other in
a straight line
anaphase
Phase 3
Spindles
spread apart
and
chromosomes
split
Sister chromatids
separate in
anaphase two, as for
anaphase one where
they stay together
Telophase 2
Phase 4
Cleavage
furrow begins
to form
After it forms then it
separates and forms
around the cell.
Illustration
Meiosis II
BIO 105 Lab 2 Mitosis, Meiosis and Gametogenesis
Photo-Micrograph
Meiosis II
BGee 130201.1
Biology 105 – Human Biology
Session:
Section:
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Spring 2013
55244 4 Units
UVC1 St. Helena
F 9:00 AM – 3:50 PM
RIDDELL
Table 3: Illustrations/Notes of cells- (Human Gametogenesis , Testis, Sperm, and Ovary
Table 3 Gametogenesis and Human Development
http://webnt.calhoun.edu/distance/internet/natural/anatomy/EndoRepro/ovary4X.html
BIO 105 Lab 2 Mitosis, Meiosis and Gametogenesis
BGee 130201.1