▶ Enzymes for NexGen Applications
▶ PCR & Amplification
▶ Cloning Kits & Vectors
▶ Competent Cells
▶ Protein Expression Kits
Simplify your research
Try Lucigen today
lucigen.com
FREE SAMPLE
The ideal combination of Performance, Convenience, and Value
lucigen.com/samples
Our polymerases represent a superb option for all your amplification needs. With excellent robustness and great
value, your lab can reduce costs without sacrificing performance.
NEW Taq98™ Hot Start 2X Master Mix
• Amplify the most challenging GC-rich templates >70%
Tough Human Templates
Taq98™ Hot Start
• Supreme ease of use – just add your primers and
template & go
GoTaq® Hot Start
Lane %GC
1
2
3
4
5
6
7
8
• Extreme Thermostability - 98°C hot start step ensures
denaturation of challenging sequences
5
6
7
8
1
Platinum® PCR Supermix
1
2
3
4
5
6
7
8
2
4
3
PLUS GREEN
B
GoTaq
PLUS GREEN
← 5 kb
• EconoTaq PLUS Green includes loading and
tracking dyes
Also available:
EconoTaq DNA polymerase with separate buffers for
greater flexibility
1
A
• Complete 2X Master mix with PCR enhancer
for robust amplification
3
5
6
7
8
AmpliTaq Gold® Master Mix
EconoTaq® PLUS and PLUS GREEN
2X Master Mixes
• Outstanding performance and value for your
every day PCR applications
2
4
5
6
7
78.0%
76.4%
75.2%
70.6%
68.6%
64.6%
59.5%
56.7%
8
C
MW
4
GoTaq
3
PLUS GREEN
2
GoTaq
1
MW
DNA Polymerases
P CR
DNA Polymerases
5 kb
3 kb
2 kb
← 75 bp
1 kb
Easy visualization with EconoTaq PLUS
GREEN. The green loading color
separates into a slow-moving blue
dye and a fast-moving yellow dye.
EconoTaq PLUS GREEN Master Mix amplified a template
GoTaq® Green Master Mix
(Promega) could not (arrow).
PCR was performed according to the manufacturer’s
recommendations. Panel A,
a prokaryotic single-stranded
DNA binding protein (genomic DNA); Panel B, prokaryotic DNA polymerase A
(genomic DNA); Panel C,
4.5 kb gene (plasmid DNA).
PyroScript® RT-PCR 2X Master Mix
• Fast, single-tube RT-PCR: amplify RNA in <30 minutes
A. Human 18S rRNA
B. Viral RNA
• Exceptional thermostability: reverse transcription at >70°C
Also available:
• EconoTaq® DNA Polymerase
• PyroPhage® 3173 DNA Polymerase (Exo-)
• Bst DNA polymerase
• PyroPhage 3173 Wild type
• NxGen™ Phi29 DNA polymerase
• NxGen M-MuLV Reverse Transcriptase
• dNTP’s and more!
100 bp 87
Ladder bp
%GC
244
bp
367
bp
262
bp
249
bp
100 bp 89 124
Ladder bp bp
93
bp
160
bp
217
bp
218 362
bp bp
243
bp
294
bp
51% 56% 54% 65% 53%
Single Enzyme RT-PCR
A. Human 18S rRNA sequences were amplified from 100 pg total A549 cell line
RNA. Five primer sets targeting amplicons of between 51 and 65% GC content
and 87 to 367 bp in length were tested. B. Viral RNA (Enterobacteriophage
MS2) was amplified by 40 cycles of RT-PCR without background. Products from
89 to 362 bp in length were amplified. One-step single-enzyme RT-PCR cycle
conditions: 15 sec @ 94°C (10s @ 94°C, 30s @ 72°C)*40.
Lucigen’s novel, transcription-free vectors reduce cloning bias, improve efficiency, and increase stability for your
most demanding cloning applications.
30kb→
5-30kb
Ori
SL1
primer
pSMART® LCAmp
← 5kb
2025 bp
CloneSmart®
SR2
primer
Blunt
EcoRI
XbaI
EcoRV
SwaI
10kb
15kb
20kb
25kb
30kb
→200kb
Cloning kits for whatever insert size you need.
Ori
CloneSmart® Cloning Kits
BigEasy® Cloning Kits
• Pre-processed pSMART® vectors for
pSMART HCAmp
the ultimate
of use Blunt
1833ease
bp
• Efficiently clone any insert up to 30kb
into unique pJAZZ® vectors
XbaI
primer stabilizes
• Transcription-free design
EcoRV
SwaI
Am
toxic inserts
p
• Maximum insert stability
• Reliably clone up to 200kb inserts –
capture entire gene clusters
• Create libraries from ultra-high A/T or
G/C-rich genomes
• High insert stability increases
sequence coverage and reduces bias
• Clone entire gene clusters or operons
• Inducible copy number
Available with:
Available with:
• Fosmid (35-45kb insert) or BAC
(up to 200kb insert) formats
SL1
primer
EcoRV
EcoRI
Blunt
®
EcoRI
SR2
NING
LO
BigEasy®
EcoRV
EcoRI
Blunt
5kb
Am
p
CopyRight®
• Ultra-low background – no need for
screening your
clones
ri
O
RO
P
Available with:
SL1
primer
EcoRV
EcoRI
• Electrocompetent or Chemically
Blunt
pSMARTcells
LCKan
competent
®
1980 bp
Blunt
EcoRI
XbaI
EcoRV
SwaI
K
• Kanamycin or Ampicillin
SR2 antibiotic
primer
resistance
an
• Blunt, NotI, or PCR product cloning
formats
• Kanamycin or Chloramphenicol
antibiotic resistance
NotI
AhdI
ApaI
(SmaI)
• High or Low Copy number
CopyRight® v2.0 Fosmid &
BAC Cloning Kits
Ori
SL1
primer
pSMART HCKan
®
1788 bp
Ka
n
SR2
primer
EcoRV
EcoRI
Blunt
Blunt
EcoRI
XbaI
EcoRV
SwaI
tel N
repA
cB
tel N
repA
cB
(SmaI)
AhdI
NotI
Cloning Systems
Clone virtually any size of insert – from routine sequences to “unclonable” DNA!
RO
P
C
Cloning Systems
Insert DNA
C
G
Insert DNA
G
C
Also available:
• GC Cloning – the more efficient alternative to TOPO TA cloning kits
A
• DNATerminator and PCRTerminator End Repair Kits
Kanr
pJAZZ-OK
Kanr
pJAZZ-OK GC
eLucidator™
Cloning Kits & Vectors
INTERACTIVE SELECTION GUIDE 
eLu
Com
INTERA
lucigen.com/cloningguide
• A full suite of enzymes to complete your cloning experiments
V I CES
SER
Lucigen Custom Library Construction Services
The recognized leader in random-shear BAC libraries!
• Unbiasedlibraries,fewergaps
•
Idealforde novo genome sequencing and metagenomic studies
•
Bundledlibraryconstructionandsequencingservicesavailable
B
C
eLucidator™
eL
Cloning Kits & Vectors
Co
INTERACTIVE SELECTION GUIDE 
INT
Cloning Kits & Vectors
eLucidator™ SELECTION GUIDE 
eLuc
Cells for Cloning
E. cloni® Competent Cells for Cloning
Phage Display Competent Cells
• Transformation efficiencies up to ≥4 ×10 cfu/µg (SUPREMES)
• Perfect for antibody or peptide phage display libraries
• Chemically competent or electrocompetent formats
• Highest transformation efficiencies available anywhere
• Replace your expensive strains with improved quality
• TG1, SS320, and E2738 strains – your only source for all three
10
• Amber suppressor and non-amber suppressor lines for
maximum flexibility
Transformation Efficiency Comparison
• Create larger libraries and speed your discovery
6 × 1010
Transformation Efficiency Comparison
5 × 1010
4 × 1010
4 × 1010
Efficency (cfu/µg)
Efficency (cfu/µg)
Competent Cells
lucigen.com/samples
Lucigen’s cells combine unparalleled transformation efficiencies and value in a range of strains and formats.
3 × 1010
2 × 1010
1 × 1010
P E TE NT
Lucigen
SUPREME
Life Tech
MegaX
3 × 1010
2 × 1010
1 × 1010
0
LL S
CO
FREE SAMPLE
Choose your application – Cloning, Phage Display, or Protein Expression.
CE
M
Competent Cells
TG1 SS320 ER2738
Lucigen
TG1
Competitor S
Cells for Protein Expression
E. cloni® EXPRESS BL21(DE3)
OverExpress™ C41(DE3) and C43(DE3)
• Great for any E. coli T7 expression vector
• Effective in expressing toxic and membrane proteins from
any organism in a T7 vector
• Chemical and electrocompetent strains available with
efficiencies ≥ 5 × 109
• pLysS and pLysE strains available
• Chemical and electrocompetent strains available with
transformation efficiencies ≥1 × 1010
• Over 350 publications attest to OverExpress performance
• Also available in pLysS strain
Custom Competent Cells
Save time and money
•
We can make your specific strain efficiently
and consistently
•
C41
BL21
C43
Expression of toxic protein. Green Fluorescent Protein was
expressed from a T7 promoter construct in C41, BL21, or C43
competent cells.
Any of our strains are available in custom
volumes – less waste, higher throughput for
your large scale experiments
A
eLucidator™
Cloning Kits & Vectors
INTERACTIVE SELECTION GUIDE 
eLucidator™
Competent Cells
INTERACTIVE SELECTION GUIDE 
lucigen.com/compcellguide
eLucidator™
Protein Express
INTERACTIVE SELECTIO
Protein Expression
NEW Expressioneering™ Technology
Your path to surprisingly simple protein expression!
• Enzyme-free directional PCR cloning in seconds
• Save days of effort with ready-to-use vectors and cells – no ligation steps
• Bacterial and Mammalian Expression systems available
E. coli Expression
Mammalian Expression
• Your choice of T7 or Rhamnose Promoter
• CMV promoter for robust expression in multiple cell lines
• Tightly controlled expression
• Smallest mammalian expression vector available
• N- or C-terminal 6X His-tagged proteins
• HA tag for easy protein purification & immuno detection
• Enhanced solubility with cleavable
SUMO tag
T
PRO EI N
EXP
R E SS
ON
I
Interested in Expressioneering?
Order the Expresso® T7,
Rhamnose, or CMV Cloning and
Expression Kits today!
Watch how
it works →
View the Expressioneering
Technology video at:
lucigen.com/expressovideo
Enyzmes
E N Z YM
NxGen™ Genomic Grade Enzymes
Exceptional performance for demanding applications
• High sensitivity for DNA/RNA sequencing & array analysis
• 99% purity for consistency and reliability
• Industry-leading value and quality
NxGen™ T4 DNA Ligase
Price Comparison
Lucigen
QuALiTy & PriCE
8¢
ES
Advantage
Competitor L*
Actual example of the
savings you can expect.
11¢
Competitor N
19¢
0
5
10
15
20
Cents/Unit
*Converted into equivalent units.
Including:
• T4 DNA Ligase
• Phi29 DNA Polymerase
• M-MuLV Reverse Transcriptase
• And many more!
Modifying Enzymes
SELECTION GUIDE 
lucigen.com/enzymesguide
Orders:
Online: www.lucigen.com
[email protected]
Phone: 608 831 9011
888 575 9695
At Lucigen, we deliver advanced
molecular biology products and
services to life scientists by inventing
solutions to the most difficult problems
in DNA cloning, amplification, and
protein expression.
FAX: 608 831 9012
Tech Support:
Phone: 608 831 9011
E-mail: [email protected]
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LucigenCorp
www.lucigen.com