DynaMag-96 Magnets - Thermo Fisher Scientific

wComp™ Dynabeads (10 mg/ml) 5 ml
DynaMag-96 Side
wComp™
Release
Buffer
2 x 20 ml
oge
number
123.31D
IT
CONTENTS
2. TECHNICAL
Products
Descriptions
5.
• Prepare approximately
10
ml SUPPORT
of isolation
buffer
cells.
7 cells.for
Mix
wellper
and
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antibodies
specific
for
mouse per
• 5x10
Prepare
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FlowComp™
Dynabeads® (10 mg/ml) 5 ml
2.2
DynaMag-96
Bottom
2.3 DynaMag-96
Side
Skirted
secondary
antibodies 2.3
specific
for of
mouse
® (10
antibodies for
flow
cytometry
staining.
• Skirted
Prepare
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2.1 DynaMag-96
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2.2
DynaMag-96
Bottom
DynaMag-96
Side
FlowComp™
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mg/ml)
5 ml
antibodies
for
flow
cytometry
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buffer
to
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byantibodies
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2.1Cataloge
DynaMag-96
Side
2.2
DynaMag-96
Bottom
2.3 Dy
CatalogeCataloge
number number
123.32D
number
120.27
for
flow
cytometry
staining.
•
Add
2
ml
cold
isolation
buffer
to
wash
cells,
followed
by
centrifuga
Release
Buffer
2 x 20 ml
123.31D
Cataloge
number
123.32D
Cataloge
number
120.27
1.FlowComp™
KIT CONTENTS
• Add 2 ml cold isolation
bu
FlowComp™
Release
Buffer
2 x 20 ml
Cataloge
number
123.31D
Cataloge number 123.32D
Catalog
Isolation procedure
for 8 min. at 350xg.
Isolation
procedure
for 8 min.
at 350xg.
abeads® FlowComp™ Human CD8
Isolation
procedure
Dynabeads® FlowComp™ Human CD8
for 8 min.
at 350xg.
Human CD8buffer and add 25 µl7
• Resuspend 5x107 Dynabeads
MNCs in® FlowComp™
500 µl isolation
RODUCT
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2.Catalog
PRODUCT
DESCRIPTION
alog no. DESCRIPTION 113.62D
no.
113.62D
•
Resuspend
MNCs25
2.Catalog
PRODUCT
DESCRIPTION
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no.
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127.5mm
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olation of CD8+ T cells from human
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wComp™
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Comp™ Human CD8 Antibody
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2.
DESCRIPTION
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DESCRIPTION
thePRODUCT
target cells.
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2.
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123.21D DynaMag-2
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protocol
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Rev.
Date:
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Date: ..................................2010
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..................................2010
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= Incubation time
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1. KIT CONTENTS
DynaMag -96 Side Skirted
DynaMag -96 Side
DynaMag -96 Bottom
DynaMag -96 Side
DynaMag -96 Side Skirted
DynaMag -96 Bottom
Product description
can be added and removed either
manually or by an automated liquid
handling robot. When the magnetic field
is removed, the Dynabeads™ magnetic
beads can be resuspended in the liquid.
In the absence of a magnetic field,
the superparamagnetic Dynabeads™
magnetic beads exhibit no residual
magnetism. This superparamagnetic
characteristic enables the beads to float
freely and individually in the sample
volume and subsequently to be collected
at the walls or bottom of the well via
magnetic field for sample separation or
buffer exchange.
With strong magnets and great
functionality, the DynaMag™ magnets
outperform all other magnetic
separators, providing faster and more
efficient magnetic separation.
See thermofisher.com/magnets for the
full magnet range.t
right-most position
to the left-most
Date: ..................................2010
= Incubation time
position.
Each
shift
in
position causes
Kund: Invitrogen
Ordernr: 61426
™
the
Dynabeads
magnetic
beads to
Artikel: 123.31D_32D_27_Rev001_Spec-06838
alternate between opposite walls
Korrektur 1: 2010-09-28
Korrektur 3: 2010-10-11
of the
well. Continuous
reciprocal
Korrektur
2: 2010-10-07
Korrektur 4: 2010-10-21
shifting
results
in
continuous
mixing.
Färg: PMS 653
Antal sidor: 2 Format: 240x256
The benefits of this type of mixing are:
No
physical interaction with the
liquid volume during mixing
Reduced
chance of contamination
Reduced
sample degradation
Kund: Invitrogen
When Ordernr:
used 61426
in combination
with the
Artikel:™
123.31D_32D_27_Rev001_Spec-06838
DynaMag
-96 Bottom magnet on an
automated
allow
for
Korrekturplatform,
1: 2010-09-28 these
Korrektur
3: 2010-10-11
Korrektur 2: 2010-10-07
Korrektur 4: 2010-10-21
significant
volume changes
within a
Färg: PMS 653
Antal sidor: 2 Format: 240x256
workflow:
Ordernr: 61426
Kund: Invitrogen
High
wash volumes Artikel: 123.31D_32D_27_Rev001_Spec-06838
(DynaMag™-96 Side or
Korrektur 1: 2010-09-28
Korrektur 3: 2010-10-11
™
DynaMag
-96 Side
Skirted)
Korrektur
2: 2010-10-07
Korrektur
4: 2010-10-21
Färg:
653 μL) elution
Antal sidor:volumes
2 Format: 240x256
Low
PMS (5
(DynaMag™-96 Bottom)
High
wash volumes (DynaMag™-96 Side or
DynaMag™-96 Side Skirted)
Low
elution volumes (DynaMag™-96 Bottom)
Reduced
risk of volume loss
• When used in combination with the
DynaMag™-96 Bottom magnet on an
automation platform, these magnets
allow for significant volume change
within a workflow:
High
wash volumes (DynaMag™-96 Side or
DynaMag™-96 Side Skirted)
Low
elution volumes (DynaMag™-96 Bottom)
For Research Use Only. Not for use in diagnostic procedures.
* Because plates may vary, users should test their plates for compability.
Precautions
These magnets contain very strong permanent magnets. People wearing a pacemaker
or any other medical magnetized implant should not use this product unless advised
by a health professional; the implant could be affected or damaged by exposure to a
strong magnetic field. Keep tools and objects that could be damaged by the magnetic
field out of the working area. This includes, but is not restricted to, credit cards and
other products containing magnetic recording devices. Keep away from delicate
instruments, watches, electronic equipment, displays and monitors. The magnet may
attract steel or other magnetic material with high mechanical forces. Take care during
handling. Avoid contact between two magnets. Do not pull the magnets apart if
contact has been made; twist off to prevent damage to the unit or fingers.
The Health and Safety Officer should take all necessary steps and full responsibility
to ensure that the precautions and statements are followed and adhered to.
Cleaning and disinfection
The following materials are recommended for cleaning purposes. Spray and/or wipe
the DynaMag™ units either with 70% isopropyl alcohol, 1% sodium hypochlorite
solution (bleach), or 0.1N HCl solution. Do not submerge in aqueous solutions and
avoid prolonged exposure to water or aqueous solutions. Clean with a damp cloth
and mild detergent when exposed to harsh solvents.
IMPORTANT: Do not autoclave DynaMag™ magnets.
Storage and stability
The magnets contains high-energy Neodymium permanent magnets. Magnetic
strength will not diminish significantly during the lifetime of the product. Do not
use the magnets above 50°C (122°F) and store in a cool, dry environment. Avoid
prolonged exposure of the magnets to direct sunlight or artificial UV light as the
surface material may become brittle.
Important licensing information
This product may be covered by one or more Limited Use Label Licenses. By use of
this product, you accept the terms and conditions of all applicable Limited Use Label
Licenses.
Manufactured by Life Technologies AS, Norway. Life Technologies AS complies with
the Quality System Standards ISO 9001:2008 and ISO 13485:2003.
Limited Product Warranty
Life Technologies Corporation and/or its affiliate(s) warrant their products as set
forth in the Life Technologies’ General Terms and Conditions of Sale found on Life
Technologies’ website at www.lifetechnologies.com/termsandconditions. If you
have any questions, please contact Life Technologies at www.lifetechnologies.com/
support.
SPEC-06838
©2015 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of
Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S)
WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR
CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT,
INCLUDING YOUR USE OF IT.
For support visit thermofisher.com/support or
email [email protected]
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