wComp™ Dynabeads (10 mg/ml) 5 ml DynaMag-96 Side wComp™ Release Buffer 2 x 20 ml oge number 123.31D IT CONTENTS 2. TECHNICAL Products Descriptions 5. • Prepare approximately 10 ml SUPPORT of isolation buffer cells. 7 cells.for Mix wellper and incubate secondary antibodies specific for mouse per • 5x10 Prepare approximately 10 ml isolation buffer 5x10 FlowComp™ Dynabeads® (10 mg/ml) 5 ml 2.2 DynaMag-96 Bottom 2.3 DynaMag-96 Side Skirted secondary antibodies 2.3 specific for of mouse ® (10 antibodies for flow cytometry staining. • Skirted Prepare approximately 10 2.1 DynaMag-96 Side • Add 2 ml cold isolation 2.2 DynaMag-96 Bottom DynaMag-96 Side FlowComp™ Dynabeads mg/ml) 5 ml antibodies for flow cytometry staining. buffer to wash cells, followed byantibodies centrifugation 2.1Cataloge DynaMag-96 Side 2.2 DynaMag-96 Bottom 2.3 Dy CatalogeCataloge number number 123.32D number 120.27 for flow cytometry staining. • Add 2 ml cold isolation buffer to wash cells, followed by centrifuga Release Buffer 2 x 20 ml 123.31D Cataloge number 123.32D Cataloge number 120.27 1.FlowComp™ KIT CONTENTS • Add 2 ml cold isolation bu FlowComp™ Release Buffer 2 x 20 ml Cataloge number 123.31D Cataloge number 123.32D Catalog Isolation procedure for 8 min. at 350xg. Isolation procedure for 8 min. at 350xg. abeads® FlowComp™ Human CD8 Isolation procedure Dynabeads® FlowComp™ Human CD8 for 8 min. at 350xg. Human CD8buffer and add 25 µl7 • Resuspend 5x107 Dynabeads MNCs in® FlowComp™ 500 µl isolation RODUCT • Resuspend 5x10 MNCs in 500 µl isolation buffer and7 add 2.Catalog PRODUCT DESCRIPTION alog no. DESCRIPTION 113.62D no. 113.62D • Resuspend MNCs25 2.Catalog PRODUCT DESCRIPTION • FlowComp™ Remove and Human discard the supernatant. no. 113.62D• Remove and discard the supernatant. • Remove and5x10 CD8 Antibody. 9 discard CD8 the s product is intended for positive magneFlowComp™ Human CD8 Antibody. 9 This product is intended for positive magnember of cells (MNC) processed: 2x10 FlowComp™ Human 127.5mm Number of cells (MNC) processed: 2x10 9 This product is intended for positive magne127.5mm Number of cells7.3(MNC) processed: 2x10 olation of CD8+ T cells from human tic isolation of CD8+ T • cellsAdd from1human + T cells from human 7.3 16.5 to tic isolation of CD8 ml cold isolation buffer the cell pellet and resuspend. wComp™ Human CD8 Antibody 1 ml 16.5 • Add 1 ml cold isolation buffer to the cell pellet and resuspend. FlowComp™ Human CD8 Antibody 1 ml and incubate for 10mmHuman min at 2 Antibody – 8°C. onuclear cells (MNCs). The supplied Add well 1 mland coldincubate isolationfor bu FlowComp™ CD8 1 ml 9.2 Mix well and incubate for 10mmmin at 2 •– 8°C. mononuclear cells (MNCs). Mix The well supplied 9.2 Mix mononuclear cells (MNCs). The supplied ® (10 mg/ml) 5 ml ® (10 mg/ml) 5 ml wComp™ Dynabeads ocol describes magnetic labeling and FlowComp™ Dynabeads ® (10 mg/ml) 5 ml protocol describes magnetic labeling and ™ FlowComp™ Dynabeads protocol describes magnetic labeling ® and ® and mix well. 85mm 85mm 7 • Add 75 µl resuspended FlowComp™ Dynabeads and mix well. 7 • Add 75 µl resuspended FlowComp™ Dynabeads ion from Release 5x10 MNCs. the2 first isolation from Release 5x10 MNCs. Addfollowed 75mlµlcold resuspended Fl • InAdd mlstep buffer to 7wash by centrifugation wComp™ Buffer In the2 first x 20 step ml 2 ml cold isolation buffer to wash••cells, byisolation centrifuga FlowComp™ Buffer x2 20 mlcold isolation MNCs. In the2followed first isolation from Release 5x10 Add 2 bu FlowComp™ Buffercells, x 20 step ml• Add Comp™ Human CD8 Antibody FlowComp™ Human CD8 Antibody (mouseat 350xg. ™ (mouse FlowComp™ Human CD8 Antibody (mouse for 8 min. at 350xg. for 8 min. for 8and min.tilting. at 15 350xg. Incubate for 15 min at 2 – 8°C under rolling at 2 – 8°C under rolling and tilting. IgG2a antibody against humanIncubate CD8) bind for to 15 min a antibody against human CD8) bind to Incubate for min at 2 – IgG2a antibody against human CD8) bind to + 2. DESCRIPTION RODUCT DESCRIPTION thePRODUCT target cells. In the second step CD8+ T arget cells. In the second step 2. DESCRIPTION T Remove and discard the supernatant. thePRODUCT target cells. In the second step CD8+ • ™ CD8 T • Remove and discard the supernatant. • Remove and discard the s cells product that have thefor antibodies • Carefully Place the remove tube in the magnet for minimum min.tube Carefully that have thefor specific antibodies •specific Place the tube inThis theproduct magnet for the minimum 1 min. This is bound intended positive magnecells that have specific antibodies product is bound intended positive magne• Place1 the in the rem ma is bound intended for positive magneare isolation captured of by CD8 the +® Dynabeads. Indiscard the last the aptured of by CD8 the +Dynabeads. In the last Human CD8+ cells isolated + cells of ® last tic T cellsand from human areCD8 captured by CD8 the +Dynabeads. In the and discard theisolation supernatant. olation T cells from human + cells ® tic isolation T cells from human supernatant. Human isolated with Dynabeads and discard theisolation supernata with Dynabeads Human CD8cold isolated with Dynabeads • resuspend. Add 1 ml buffer to the cell pellet and resuspend. • Add 1 ml cold isolation buffer to the cell pellet and • Add 1 ml cold bu step beads are removed from the cells. mononuclear cells (MNCs). The supplied beads are removed from the cells. step beads are removed from the cells. onuclear cells (MNCs). The supplied FlowComp™ Human CD8 mononuclear FlowComp™ Humancells CD8 (MNCs). The supplied FlowComp™ Human CD8 123.21D DynaMag-2 protocol describes magnetic labeling and protocol describes magnetic labeling and col describes magnetic labeling and12331D, 12332D • Remove the tube from the magnet. Add at least 1 ml cold isola Catalog nos. 12027, ® the • Remove tube from th • Remove the tube from the magnet. Add at least 1 ml cold isolation ® 7 • Add 75 µl resuspended FlowComp™ Dynabeads and mix well. Downstream Applications 7 MNCs. • Add 75 µl resuspended Flo 7 MNCs. In the first step • InAdd 75 µl FlowComp™ Dynabeads andbuffer mix well. MNCs. the first stepresuspended isolation from 5x10 Downstream Applications nstream Applications In the first step isolation from 5x10 ion from 5x10 and5 resuspend the bead-bound cells by gentle pipetting the 5 tim buffer and resuspend buffer(mouse and thecells bead-bound cells by(mouse gentle pipetting times. DynaMag-2 123.01D DynaMag-15 Antibody 123.01D DynaMag-15 FlowComp™ Human CD8 Antibody Isolated cellsHuman may beCD8 used directly in anyresuspend 123.01D DynaMag-15 FlowComp™ Comp™ Human CD8 Antibody (mouse Isolated may be used directly in any ted cells may be used directly in any Pub. No. MAN0014604 • Concentrates the beads at the side of each well. • Concentrates the beads at the bottom of each well • Concentrates the beads pellets at the side of each well. • Concentrates the the beads at the side of well. Concentrates at the bottom ofand eachtilting. well Conce ncentrates the beads at the side of each well. • Concentrates the beads at thehuman bottom of each well •at Concentrates beads pellets ateach the of each well. Incubate for• 15 min at 2the – beads 8°C under rolling IgG2a antibody against CD8) bind to 15 min Incubate for 15 min •at 2– 123.20D DynaMag-Spin IgG2a antibody against human CD8) bind to side application such as flow cytoIncubate for 2 •–volume 8°C under rolling and tilting. application such as flow cyto123.02D DynaMag-50 a antibody against human CD8) to 123.20D 123.02D DynaMag-50 •downstream Working volume 5-200 µl Working volume: 5-200 μl •volume: Working volume µlPlace nstream application such as flowbind cytoHolds: 16 standard ml •downstream Working 5-200 µl • Working 5-200 μl 5-200 • Worki DynaMag-50 • DynaMag-15 Place the remove tube in •the magnet minimum 1microcenmin.tube Carefully rem + T • 1.5 the in the ma •target Working volume µlDynaMag-Spin rking volume 5-200 µl • Working volume: 5-200Inμl + T ™ ™ ™ for the target the 123.02D second step CD8 •products Place the tube inthe the magnet minimum 1 min. Carefully + T DynaMag-15 Infor the5-200 second stepand CD8 metry. For cells. recommended and prometry. For cells. recommended products proarget In the second stepand CD8proDynaMag-15 y. For cells. recommended products Rev. Date: September 2015 (Rev. A.0) trifuge tubes and discard the in supernatant. and discard the supernata cells have bound the specific • Carefully Place the tube the magnet for minimum 1 the min.tube Carefully rem cells that have bound the specific • Place in the ma tocolsthat visit www.invitrogen.com/immunolotocols visit www.invitrogen.com/immunoloandantibodies discard supernatant. have bound the specific antibodies • Place the tube in the for minimum Holds: Holds: DynaMag-50 •the Holds: 4 x magnet standard 5 ml and 15 antibodies ml1 min. Holds: Holds: 5Holds: Holds: sthat visit www.invitrogen.com/immunoloDynaMag-50 • Holds: 4 x remove standard ml and 15 mlDynaMag-Spin Holds: s: Holds: are DynaMag-50 • Holds: 4 x standard 5PCR ml and 15 captured by ml the Dynabeads. In the last Human are captured bystrips theDynaMag-Spin Dynabeads. In the • •Working volume: µldiscard the supernata + cells isolated ® last + cells ® 10-1000 and discard supernatant. and aptured by the Dynabeads. In the last Human CD8+ cells isolated CD8 with Dynabeads •gy. Strips • PCR 96-well skirted Human CD8 isolated with12332D Dynabeads •gy. PCR Strips •the PCR strips • 96-we ® tubes •PCR-plates* Holds: 6 x 2standard 1.5 mlml tubes and discard the supernatant. Cat. no. 12331D Cat. no. 12027 Cat. no. with Dynabeads R Strips • PCR strips • 96-well skirted PCR-plates* • Holds: 2 x standard 50 ml tubes step beads are removed from the cells. • Holds: x standard 50 tubes step beads are removed from the cells. • Remove the tube from the magnet and carefully resuspend the be tubes • Remove the tube from • Holds: 6 x standard 1.5 ml 2 x standard 50 •ml tubes FlowComp™ Human CD8PCR style • 96-well PCR-plates* • Holds: •PCR-plates* 96-well plates* • Can be plates* used to holds work with beads are removed from the cells. FlowComp™ Human CD8 96-well • 96-well PCR style • Canthb • Remove the• tube from magnet andwith carefully resuspend the beadThe DynaMag-2 up culture to 16 plates. microFlowComp™ Human CD8 well PCR-plates* • 96-well PCR style plates* • volume Canthe be requirements used tomlwork culture •plates. Working 1-15 Store at ambient temperature • Remove Working volume ml- 96-well • bottom Working volume: 10-1000 µl Additional requirements Additional bound cells in 11-15 ml FlowComp™ Release Buffer. bound cells in 1 cold ml FlowC Working volume: 5-50 ml • bottom Working volume: 5-50 ml • the from the magnet. Add at least 1 ml -ml half skirted (200µl)at• the - half-skirted (200 μl) round plates • Remove the tube from th -FlowComp™ half• skirted (200µl) -tube half-skirted (200 μl) -isola 96-w • Working volume 1-15 • Working volume: 10-1000 µl • Beads collect side of each well. Beads collect at the side of each well. tional requirements • Beads collect at the of each well. centrifuge tubes and gives you a very bound cells in 1 ml Release Buffer. Working volume: 5-50 ml • Remove the tubeDownstream from thebuffer: magnet. at least 1 mlmagnet cold isolation Downstream alf skirted (200µl) - half-skirted (200 μl)Applications - 96-well round bottom plates Applications 2+Add non-skirted non-skirted -the 96-well flat bottom plates • -I solation buffer: Ca2+ and Mg2+ free This magnet -the non-skirted - be non-skirted • -I solation Caby can- be rotated 360 and deMg2+ The free This rotated bybead-bound 360 deThisThe magnet fits in96-w t nstream Applications buffer andincan resuspend cells by pipetting 5 tim buffer andcomfortably resuspend unit is ergonomically designed, alis gentle ergonomically designed efficient separation ofunit proteins and -the 96-well flat bottom plates on-skirted non-skirted olation buffer: Ca2+ and Mg2+ free This magnet can- be rotated bycells 360may deThis magnet fits comfortably the buffer and resuspend bead-bound cells by gentle pipetting 5 times. Isolated be used directly in any Isolated cells may be used directly in any The unit is ergonomically designed, al• Working volume: 5–200 µL. • Working volume 5–200 µL. Incubate for 10 min. in room temperature under rolling and tilting Incubate for 10 min. in ro 24-well plates phosphate buffered saline (PBS) from 24-w phosphate buffered saline (PBS) from • Working volume 5–200 µL. grees, giving you the flexibility to work grees, giving you the flexibility to work palm of your hand and a ve This magnet features 7 bar magnets with a hard plasThis plate magnet features 96 round magnets (with This plate magnet features 7 bar magnets with a10 hard plas-plate This plate magnet features 96lowing round magnets (with one lowing you magnet to rest your elbows on you to tilting. rest your elbows on the The ted cells may be used directly any for your min. room temperature under rolling and nucleic acids. lightweight isonegives -in24-well plates osphate buffered saline (PBS)in from flexibility to work palm ofml. your hand andto gives avolumes very downstream application such as flow cytodownstream application suchfitIncubate as flow cytoplate magnet features 7 bar magnets withgrees, a hard giving plas- you Thisthe plate magnet features 96stable round magnets (with onesample 12-w Gibco (cat.no. 14190-094) supplemented 12-well plates Gibco (cat.no. 14190-094) supplemented lowing you to rest elbows on the with sample up to 15 ml. You with volumes up to 15 You tic top witch ensures a stable fit for 96-well PCR-plates. This centred at the bottom of each well) to ensure identical tic top witch ensures a for 96-well PCR-plates. This centred at the bottom of each well) ensure identical efficient separation of proteins and n • with Place1 the the rem ma • Place and the tube the magnet forhold, minimum min.tube nstream application suchfitsupplemented as cytoworkbench and tiltCarefully thein magnet workbench tiltnuthein magnet toplates a to - 12-well plates bco (cat.no. 14190-094) comfortable numbered For recommended products and prometry. For recommended and prowith sample to 15 ml. You p witch ensures a stable forflow 96-well PCR-plates. This volumes centred up at the bottom of each well) ensure efficient separation proteins and - 6-w with 0.1% BSA and 2mM EDTA (see - 6-well with 0.1% BSA and 2mM EDTA (see •products Place the tube inmetry. the magnet for minimum 1 Carefully remove • This Mix the cells by gentle pipm Mix the cells by gentle pipetting 5position. times and place the tube in volum the workbench and tilt the magnet athe magnet collects Dynabeads at one the sidemin. of•of the wells. With sample handling aton each magnet pulls the magnet collects the Dynabeads attothe side ofidentical the wells. With sample handling aton each position. This magnet pulls the can work with 5the ml flow tubes one can work with 5 to ml flow tubes Holds: cleic acids from small sample Holds: y. recommended and proand discard the supernata and discard supernatant. convenient angle during use. convenient angle during use. slowly - Holds: 6-well plates th For 0.1% BSA and products 2mM EDTA tocols visitconcentrated www.invitrogen.com/immunolotocols visit www.invitrogen.com/immunoloMix the by gentle pipetting 5cleic times and place the tube in1 the magsample for your convenience. 8plates -By Nots Technical Support for further informatinet collects the Dynabeads at the side(see of the wells. With handling at each This pulls the -By Not recommended for use with 48-well Support for further informatican work with sample 5 ml flow tubes on oneposition. acids from small sample volumes the beads at the side of the wells, all residual Dynabeads to the spaces bottom of each well, allowing for elution theTechnical beads concentrated at •the sidemagnet of thecells wells, all residual Dynabeads to the bottom of each well, allowing for elution and discard the supernatant. net for 1 min. convenient angle during use. By slowly net for min. ™ rack, or simply flip it to use 15 ml tubes rack, or simply flip it to use 15 ml tubes due to the angle of the magnetic schnical visit www.invitrogen.com/immunoloturning therack tubes in the magnetfie d - • Not96-well recommended for PCR-plates* use with 48-well plates Support for further informatiturning the tubes inin the magnet during DynaMag magnets arerack, designed to Dynabeads gy. on). on). eads concentrated at the side of the wells, all residual to thebe bottom of without each well, allowing elution spaces on each side of the make net 1for min. • PCR Strips skirted •flipped, PCR strips or simply flip it togy. use 15 ml tubes fluid can be without theof bead pellet. very small volumes. Thistub pla due to disturbing the angle the magnetic field. fluid can removed disturbing the bead pellet. in removed very small volumes. This plate magnet features 6 bar-magnet which collect the onRemove the other. Once the magnet turning thefor tubes in during onthe themagnet other. Once flipped, the magnet Holding up to 6you microcentrifuge • carefully Remove the tube from th •column the tube from the magnet and resuspend theconbe ). be removed without disturbing the bead pellet. separation can concentrate separation you can concentrate the can in very small volumes. This plate magnet features 6 bar-magnet which collect the The DynaMag-96 Side magnet has an extra (13 in Dynabe this magnet scaleable with 96 well • Mixer allowing both tilting and rotation. The DynaMag-96 Side magnet has an extra column (13 in Dynabeads at the side of the well. With the bead • Mixer allowing both tilting and rotation. provide optimal magnetic separation on the other. Once flipped, the magnet Holding up to 6 with microcentrifuge tubes • Transfer the supernatant When used in combination with DynaMag-96 Side or • Transfer the supernatant containing the bead-free cells to a new • Remove the tube from the magnet and carefully resuspend the beadWhen used in combination DynaMag-96 Side or will remain in position until adjusted. separation you can concentrate the will remain in position until adjusted. in numbered spaces, the circular tt Additional requirements Additional requirements bound cells in 1 ml FlowC bead pellet on the tube wall. • 96-well PCR-plates* • Compatible with culture plates bound cells intube 1 ml FlowComp™ Release Buffer. pellet on the tube wall. • 96-well PCR style plates* DynaMag-96 Side magnet has an extra column (13 in in When Dynabeads at MPC™): the side ofbead-free the well.bead With the bead conxer allowing both tilting and rotation. total). This design feature enables sample mixing simply centrat total). design feature sample mixing simply centrated at the side of the well, all residual canthe be • enables Transfer the containing the to athe new sample plates, for subsequent automaused inThis combination with DynaMag-96 Side orml DynaMag -96the Side Skirted on an automation platform, will remain position adjusted. • FlowComp™ Magnet (Dynal See www.invitro™ DynaMag -96 numbered Side Skirted on ancells automation platform, in spaces, the circular top • until Magnet (Dynal MPC™): www.invitroand place the tube in requirements Flipping during separation 2+ and place the tube in magnet for 1 min to remove allfluid residual Flipping magnet during separation bound cells insupernatant 1the Release Buffer. bead pellet on the tube wall. rack can be quickly removed from t 2+ See of MPC™): Dynabeads magnetic beads from DynaMag •bythe I solation buffer: Ca and Mg2+forth free .tional This(Dynal design feature See enables sample mixing simply centrated at96-well the side ofback the well, all residual canmagnet be •by-96 I solation buffer: Ca and Mg2+ free shifting the plate and from thefluid rightremove shifting the 96-well plate back and forth from rightremoved without disturbing the bead pellet. With a strong magnetic field, this With a strong magnetic field, this magSide Skirted on an automation platform, these magnets allow for significant volume changes within agnet www.invitrogen.com/magnets-selection for magnet tion. these magnets allow for significant volume changes within gen.com/magnets-selection for magnet and place the tube in the magnet for 1 min to remove all residual berack canposition. be removed from the 2+ and Mg2+ free Flipping the magnet during Half-Skirted separation (200 μL) 96-well without round bottom plates will youforcollect any bead-bound Half-Skirted This (200 μL)in ads. willin help you collect any bead-bound magnet the base, ready for vort ads. Incubate for 10 min. in ro phosphate saline (PBS) from olationthe buffer: Ca Incubate 10 min. in room temperature under rolling and tilting most position tobuffered the left-most Each shift in help position This ms phosphate saline (PBS) from hifting 96-well plate backfor andmagnet forth matrices. from the rightremoved disturbing thequickly bead pellet. most position tobuffered the left-most position. Each shift position magnet can be used for mixing the same way as With a strong magnetic field, this magnet gives quick and efficient cell a workflow: these magnets allow for significant volume changes within recommendations. net gives quick and efficient cell sepadiverse liquid sample n.com/magnets-selection a workflow: recommendations. willin help you collect causes any bead-bound cells that may have become stuck inxing, magnet in for the base, ready for vorteads. The sample rack canration be quickly removed Gibco (cat.no. 14190-094) supplemented that may have become stuck inthe Incubate forcells 10 min. in room under rolling and tilting. spinning manual-96 sample sha osphate saline (PBS) Each fromshift causes the Dynabeads to beused pulled to opposite tube describ Gibco (cat.no. 14190-094) supplemented position tobuffered the left-most position. position This magnet can be mixing the same way as the Dynabeads tonet be pulled toquick the opposite tube wall. for DynaMag-96 Side. • High wash volumes (DynaMag-96 Side or or DynaMag gives and efficient cell sepafrom viscous samples suc a workflow: Non-Skirted cytometry temperature flat bottom plates •96-well High wash volumes (DynaMag-96 Side orwall. DynaMag -96 commendations. Non-Skirted described • ecommended Flow cytometry antiration from viscous samples suchtubes as ™ • ecommended Flow cytometry anti• R Flow cytometry antibody reagents • R Flow reagents (optiotube Slowly turning the ining. • and Transfer the supernatant c cellstube that have become stuck inantibody with 0.1% BSA and 2mM EDTA xing, or manual sample shakContinuous reciprocal shifting results a (see continuous mixing Dynabeads magnetic beads can be used bcothe (cat.no. 14190-094) supplemented the-96 tube lid. Slowly turning thespinning tubes inin(optioMix theDynaMag-96 cells by gentle pip Transfer the supernatant containing the bead-free cells to ainnew tu from the magnet allowing for washing with 0.1% BSA and 2mM EDTA (see You can perform your entire exp es Dynabeads to be pulled to the opposite wall.may described for DynaMag-96 Side. Continuous reciprocal shifting aviscous continuous mixing • the Mix thelid. cells by pipetting times place the tube the m Sidegentle Skirted) When u • High wash volumes (DynaMag-96 Side orinDynaMag whole blood. The DynaMag-50 ca Side Skirted) When used in 5 combination with Bottom on body reagents CD3-Alexa Fluor 488 rationresults from samples such as commended Flow cytometry antiwhole blood. The DynaMag-50 can be body reagents CD3-Alexa Fluor 488 nal): see www.invitrogen.com/immunoloow cytometry antibody reagents (optional): see www.invitrogen.com/immunoloTechnical Support for further informatithe magnet will concentrate the bead the tube lid. Slowly turning the tubes in Transfer thethe supernatant containing the bead-free cells tofor ainnew tube. Technical Support for of further informatith 0.1% BSA shifting and 2mM EDTA Add 2 types ml isolation buffe ing. You can perform your entire expemagnet will concentrate the bead nuous reciprocal results in a (see continuous mixing riment in the magnet, just remove t and vortexing with the tubes in situ. • Mix the cells by gentle pipetting 5 times and place the tube the magnet for 1 min. 6-, 12-, and 24-well plates The benefits of this type of mixing are: • Low (5μl) elution volumes (DynaMag -96 Bottom) an auto Add 2 ml isolation buffer followed by centrifugation for 8 min net 1 min. (Cat. No. MHCD0320), CD8-PE (Cat. No. The benefits of this type mixing are: • Low (5μl) elution volumes (DynaMag -96 Bottom) an automated platform, these alow for significant volume Side Skirted) When used in combination with DynaMag-96 Bottom on used with all of Dynabeads. to isolate cells, proteins, nucleic acids, or gy forcan recommended products and proto•(Cat. plate magnet features 7The barDynaMag-50 MHCD0320), CD8-PE (Cat. No. whole blood. be dy reagents CD3-Alexa Fluor 488 • This plate magnet features 96 round usedpellet with all types of gyThis forNo. recommended products and on). l): see www.invitrogen.com/immunoloon the tube wall. on). chnical Support for offurther informatithe magnet will concentrate the bead (DynaMag riment the magnet, justfor remove theDynabeads. • No physical interaction with the liquid volume during the pellet on the tube wall. 350xg. resuspend beads – change you c Add 2 protomlmin. isolation buffer followed byin centrifugation 8volume min. at The magnet can be to used with all the types MHCD0804). • No physical interaction with with the liquid the changes within aunit workflow net for 1volume 350xg. benefits of this type mixingand are:proto• Low (5μl) elution volumes -96 Bottom) an automated platform, these alow significant cols. MHCD0804). at. MHCD0320), CD8-PE (Cat. No. all types ofduring Dynabeads. cols. forNo. recommended products Not both recommended forfor use with ). • mixing Mixer allowing tilting and rotation. other biomolecules. procedure •new High magnets with aused hard plastic magnets (onevolumes magnet centered at the pellet onthe the tube wall.• mixing •store Transfer the supernatant Mixer allowing both tilting and rotation.topThe unit unit to resuspend the beads can even the magnet with your sam The unit –isyou ergonomically designed, alprocedure •Dynabeads. High wash (DynaMag-96 Side and Sidea Skirtet) physical interaction with the liquid volume during changes within a workflow 350xg. • Transfer the supernatant containing the bead-free cells to t is ergonomically designed, al• R ecommendations for evaluation of NOTE: This new product replace HCD0804). s. • Recommendations for evaluation of NOTE: This new productofreplaces the volumes • Reduced chance for contamination • Low • Discard the supernatant • Magnet (Dynal MPC™): See www.invitro• Reduced chance for contamination • Low elution (DynaMag-96 Bottom) xer both tilting and rotation. The unit is ergonomically in1to amin cold room. The magnet can lowing you tothe rest your elbows on theples even store the magnet with your samand place the tube in theae � notes ing allowing procedure •Critical High wash volumes (DynaMag-96 Side and Side Skirtet) 48-well plates • Discard the supernatant and resuspend the cell pellet in preferred • Magnet (Dynal MPC™): See www.invitroviability is SYTOX Red (Cat. No. S34859). • Transfer the supernatant containing the bead-free cells to a new tube lowing you to rest your elbows on the and place tube in the magnet for to remove all residual designed, alto ensure a stable fit for 96-well Dynal MPC™-50 magnet. � Critical notes bottom of each well) ensure identical viability is SYTOX Red (Cat. No. S34859). ™ Dynal MPC™-50 magnet. commendations for evaluation of NOTE: This new product• Reduced replaces the volumes sample degradation for magnet Mix Dynabeads magnetic beads and gen.com/magnets-selection • Reduced sample degradation medium. Keep the cells o workbench and tilt the magnet to a duced chance for contamination • Low elution (DynaMag-96 Bottom) used with all types of Dynabeads. gen.com/magnets-selection for place magnet ples cold The magnet can be This2 new product replacesuse the in downstr •magnet Discard the supernatant and resuspend the cell pellet in cell agnet (Dynal medium. Keep theNOTE: cells on – 8°C untilads. further Use a mixer provides rotaworkbench and tilt that the magnet to and aroom. elbows on for thevolume and the tube in the magnet forinloss 1atilting min to remove allpreferred residual beritical notesMPC™): See www.invitro- lowing you to rest your ads. Use a mixer provides tilting and rotability is SYTOX Red (Cat. No. S34859). •• Reduced risk for volume Dynal MPC™-50 magnet. recommendations. PCR-plates. The collects the sample handling at each position. •• Reduced risk that loss *Because plates may vary,applications. users should test their*Becau plates duced sample degradation convenient angle during use. MPC™-S •ofThis plate features 6Dynabeads. bar with all types of tion the tomagnet ensure that Dynan.com/magnets-selection for a magnet Dynal magnet. your sample in well ofworkbench a 96-well plate, convenient angle during use. applications. medium. Keep the cells on 2tubes – used 8°C until further use in downstream and tilt therecommendations. magnet a™ to ensure tion of the to tubes that Dynafor com ads. e a mixer provides tilting and rotaWhen used in combination with DynaMag-96 Bottom onplates for compability NOTE: ™ supernatant • Flow cytometry antibody reagents (optioduced risk that for volume loss *Because plates may vary, users should test their When used in combination with DynaMag-96 Bottom on beads do not settle at the bottom of the This new product replaces tc • Transfer the commendations. • Flow cytometry antibody reagents (optioDynabeads magnetic beads at the The magnet pulls the Dynabeads With a strong magnetic field, this magbeads do not settle at the bottom of the • Transfer the supernatant containing the bead-free cells to a new tu convenient angle during use. applications. magnets. The magnet collects an automation platform, these magnets allow for signifin of the tubes to ensure that DynaWith a strong magnetic field, this magallowing the beads to bind the target nal): see www.invitrogen.com/immunoloannal): automation platform, these magnets allow for signififor compability tube. MPC™-S see www.invitrogen.com/immunolonads used with DynaMag-96 Bottom on Add 2magnet. ml isolation buffe tube. NOTE: This new product replaces the net gives quick and efficient cellbeads sepa-Dynal ow cytometry antibody reagents (optiocant volume changes within a workflow: Add 2 ml isolation buffer followed by centrifugation for 8 min do in notcombination settle at the bottom of the With • Transfer the supernatant containing the bead-free cells to a new tube. net gives quick and efficient cell sepaside of the wells. With the beads ™ magnetic to the bottom of each gy for recommended products and protochanges a workflow: asignifistrong magneticcant field, mag- within For350xg. further technical advice p thevolumes Dynabeads beads gy volume for this recommended products and protoutomation platform, these magnets • High This wash product should notMPC™-S bemagnetic used with biomolecules within allow a fewforminutes. further technical advice please visit www.invitrogen.com/cellisolat ration viscousat samples such as Dynal magnet. l): see www.invitrogen.com/immunolo(DynaMag-96 orFor DynaMag -96 This wash product should not Add be used with be. cols. 350xg. ration •from viscous samples asSide •• High (DynaMag-96 Side or wells, DynaMag -96 2 ml isolation buffer followed bysuch centrifugation forfrom 8 min. net gives quick and efficient cell volumes sepacols. magnet MPC-1. volume changes within a workflow: concentrated at the side of the well, allowing Side Skirted) for recommended products and field proto- is applied to the whole blood. The DynaMag-15 can befor elution in very small magnet MPC-1. For further technical advice please visit www.invitrogen.com/cellisolation. at the side of the wells. With the When a magnetic Side Skirted) whole blood. The DynaMag-15 can be is product should not be used with 350xg. ration from hs. wash volumes (DynaMag-96 Side or DynaMag -96 viscous samples such as • Low elution volumes (DynaMag-96 Bottom)used with all types of Dynabeads. • in Discard the supernatant � Critical notes • Low volumes (DynaMag-96 Bottom)used with allelution residual can be removed volumes. When used combination Discard the supernatant and resuspend the cell pellet intime preferreda = Incubation all types of Dynabeads. agnet MPC-1. � Critical notes beads concentrated at the• side e Skirted) whole The DynaMag-15 can befluid = of Incubation time plate, the beads are gently andblood. rapidly medium. Keep the cells o • Use a mixer that provides tilting and rota™ medium. Keep the cells on 2 – 8°C until further use in downstre w elution volumes (DynaMag-96 Bottom) • Discard the supernatant and resuspend the cell pellet in preferred cell • Use a mixer that provides tilting and rotawithout disturbing the bead pellet.time tion of the with all types of Dynabeads. with replaces theDynaMag -96 applications. Side or ritical notes NOTE: the well, residual fluid be This new product =that Incubation theproduct tubes all toreplaces ensure that Dyna-can attracted to the walls orused bottom of NOTE: This new the applications. tion of the tubes to™ ensure Dynamedium. Keep cells do onnot 2 – 8°C until further in downstream ™ Dynal MPC™-L andDynaMag Dynal MPC™-15 beads at the bottom of theuse e a mixer that provides tilting and rotaThe do DynaMag magnet hastheMPC™-L -96 Side Skirted magnets on and settle Dynal MPC™-15 beads not the settle at-96 theSide bottom of the Dynal removed without disturbing the the well, alongthat with theNOTE: bead-bound This new product replaces tube. applications. magnets. n of the tubes to ensure Dyna123.31D_32D_27_Rev001_Spec-06838.indd 1 tube. magnets. an extra column (13 in total) to enable an automated platform, these magnets 123.31D_32D_27_Rev001_Spec-06838.indd 1 10 Dynal pulled MPC™-L and Dynal MPC™-15 For further technical 10-10-21 advice p 113.62D.indd 1 bead pellet. Thisbemagnet can be usedtechnical advice please visit ads do notbiomolecules. settle at the bottom the beads Withofthe • This product should not used with For further www.invitrogen.com/cellisolat 113.62D.indd 1 08-10-14 Sign: .......................................... • This product should not be used with be. magnets. Sign: .......................................... sample mixing by simply shifting the magnetfor MPC-1. allow for significant volume changes 32D_27_Rev001_Spec-06838.indd 1 10-10-21 10.41.00 mixing the same way described magnet MPC-1. to the walls or bottom of the wells, it is For further technical advice please visit www.invitrogen.com/cellisolation. D.indd 1 08-10-14 10.27.03 Date: ..................................2010 is product should not be used with ..................................2010 96-well plate back and forth from the within a workflow: Sign: .......................................... = Incubation time for the DynaMag™-96Date: Side magnet. easy to remove the supernatant. Liquid agnet MPC-1. = Incubation time 1. KIT CONTENTS DynaMag -96 Side Skirted DynaMag -96 Side DynaMag -96 Bottom DynaMag -96 Side DynaMag -96 Side Skirted DynaMag -96 Bottom Product description can be added and removed either manually or by an automated liquid handling robot. When the magnetic field is removed, the Dynabeads™ magnetic beads can be resuspended in the liquid. In the absence of a magnetic field, the superparamagnetic Dynabeads™ magnetic beads exhibit no residual magnetism. This superparamagnetic characteristic enables the beads to float freely and individually in the sample volume and subsequently to be collected at the walls or bottom of the well via magnetic field for sample separation or buffer exchange. With strong magnets and great functionality, the DynaMag™ magnets outperform all other magnetic separators, providing faster and more efficient magnetic separation. See thermofisher.com/magnets for the full magnet range.t right-most position to the left-most Date: ..................................2010 = Incubation time position. Each shift in position causes Kund: Invitrogen Ordernr: 61426 ™ the Dynabeads magnetic beads to Artikel: 123.31D_32D_27_Rev001_Spec-06838 alternate between opposite walls Korrektur 1: 2010-09-28 Korrektur 3: 2010-10-11 of the well. Continuous reciprocal Korrektur 2: 2010-10-07 Korrektur 4: 2010-10-21 shifting results in continuous mixing. Färg: PMS 653 Antal sidor: 2 Format: 240x256 The benefits of this type of mixing are: No physical interaction with the liquid volume during mixing Reduced chance of contamination Reduced sample degradation Kund: Invitrogen When Ordernr: used 61426 in combination with the Artikel:™ 123.31D_32D_27_Rev001_Spec-06838 DynaMag -96 Bottom magnet on an automated allow for Korrekturplatform, 1: 2010-09-28 these Korrektur 3: 2010-10-11 Korrektur 2: 2010-10-07 Korrektur 4: 2010-10-21 significant volume changes within a Färg: PMS 653 Antal sidor: 2 Format: 240x256 workflow: Ordernr: 61426 Kund: Invitrogen High wash volumes Artikel: 123.31D_32D_27_Rev001_Spec-06838 (DynaMag™-96 Side or Korrektur 1: 2010-09-28 Korrektur 3: 2010-10-11 ™ DynaMag -96 Side Skirted) Korrektur 2: 2010-10-07 Korrektur 4: 2010-10-21 Färg: 653 μL) elution Antal sidor:volumes 2 Format: 240x256 Low PMS (5 (DynaMag™-96 Bottom) High wash volumes (DynaMag™-96 Side or DynaMag™-96 Side Skirted) Low elution volumes (DynaMag™-96 Bottom) Reduced risk of volume loss • When used in combination with the DynaMag™-96 Bottom magnet on an automation platform, these magnets allow for significant volume change within a workflow: High wash volumes (DynaMag™-96 Side or DynaMag™-96 Side Skirted) Low elution volumes (DynaMag™-96 Bottom) For Research Use Only. Not for use in diagnostic procedures. * Because plates may vary, users should test their plates for compability. Precautions These magnets contain very strong permanent magnets. People wearing a pacemaker or any other medical magnetized implant should not use this product unless advised by a health professional; the implant could be affected or damaged by exposure to a strong magnetic field. Keep tools and objects that could be damaged by the magnetic field out of the working area. This includes, but is not restricted to, credit cards and other products containing magnetic recording devices. Keep away from delicate instruments, watches, electronic equipment, displays and monitors. The magnet may attract steel or other magnetic material with high mechanical forces. Take care during handling. Avoid contact between two magnets. Do not pull the magnets apart if contact has been made; twist off to prevent damage to the unit or fingers. The Health and Safety Officer should take all necessary steps and full responsibility to ensure that the precautions and statements are followed and adhered to. Cleaning and disinfection The following materials are recommended for cleaning purposes. Spray and/or wipe the DynaMag™ units either with 70% isopropyl alcohol, 1% sodium hypochlorite solution (bleach), or 0.1N HCl solution. Do not submerge in aqueous solutions and avoid prolonged exposure to water or aqueous solutions. Clean with a damp cloth and mild detergent when exposed to harsh solvents. IMPORTANT: Do not autoclave DynaMag™ magnets. Storage and stability The magnets contains high-energy Neodymium permanent magnets. Magnetic strength will not diminish significantly during the lifetime of the product. Do not use the magnets above 50°C (122°F) and store in a cool, dry environment. Avoid prolonged exposure of the magnets to direct sunlight or artificial UV light as the surface material may become brittle. Important licensing information This product may be covered by one or more Limited Use Label Licenses. By use of this product, you accept the terms and conditions of all applicable Limited Use Label Licenses. Manufactured by Life Technologies AS, Norway. Life Technologies AS complies with the Quality System Standards ISO 9001:2008 and ISO 13485:2003. Limited Product Warranty Life Technologies Corporation and/or its affiliate(s) warrant their products as set forth in the Life Technologies’ General Terms and Conditions of Sale found on Life Technologies’ website at www.lifetechnologies.com/termsandconditions. If you have any questions, please contact Life Technologies at www.lifetechnologies.com/ support. SPEC-06838 ©2015 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT. For support visit thermofisher.com/support or email [email protected] thermofisher.com
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