AdvanCE™ FS96
dsDNA Fragment Analyzer
A D V A N C E D A N A LY T I C A L
>I N S T R U M E N T C A PA B I L I T I E S
SUPERIOR INSTRUMENT, SUPERIOR RESULTS
The AdvanCE™ FS96 system has been designed for flexible operation, covering a wide range of DNA/RNA sizes and
concentrations with high separation resolution. Different gel kits are interchangeable with the same capillary array.
Detect SSRs With Confidence — Down to 2 bp Resolution
Separate Consistently Over a Wide DNA Range
200
2711
2500
80000
70000
500
2000
RFU
50000
40000
1500
113
115
1000
800
500
900
600
10000
122
1000
400
300
20
20000
100
30000
700
RFU
60000
35
90000
300
97242.347
0
34:00
36:00
39:00
42:00
45:00
48:00
51:00
54:00
57:00
01:00
35
75
50
100
150
Time
200
300
Size
DNF-910 Gel: 20 bp Extended Range DNA Ladder (Lonza®); 50 bands from
20 bp – 1,000 bp.
DNF-900 Gel: Separation demonstrating resolution of 113 bp, 115 bp, and
122 bp DNA fragments.
Resolve Large DNA Fragments
Automate Mutation Detection Analysis
Detect induced (and natural) point mutations in genomic
DNA. High detection sensitivity (as low as 5 pg/μL) combined
with a simplified cleavage process (no desalting step or EDTA
addition) result in the efficient detection of mutations. The
streamlined cleavage process and fast separation times
yield improved throughput over manual methods.
3000
2399
2200
2000
1800
4000
5000
6000
900
800
700
600
400
400
300
100
600
200
800
1500
1000
2000
500
1200
1000
200
39:00
42:00
45:00
48:00
51:00
54:00
57:00
60:00
63:00
66:00
69:00
72:00
75:00
78:00 80:00
415
35:00
14000
Time
12000
10000
8000
1000
35
RFU
DNF-920 Gels: Mixture of GeneRuler™ 100 bp DNA Ladder (Fermentas) and
1 Kb DNA Ladder (New England Biolabs®)
632
16963
16000
-80
217
RFU
1400
8000
10000
1600
6000
4000
Separate and Quantify Genomic DNA
2000
30:00
32:00
34:00
36:00
38:00
40:00
42:00
44:00
46:00
48:00
50:00
52:00
54:00
56:00
Time
Pooled DNA (equivalent of 8 diploid organisms), amplified, cleaved and separated.
The uncut fragment is 632 bp; cut fragments are 415 bp and 217 bp.
Hind III λ
Analyze Total RNA Quality
No exchange of capillary array is required, streamlining
operation and reducing costs compared to alternative
methods.
Intact
Other Applications
Degraded
10:00
15:00
20:00
25:00
30:00
35:00
40:00
45:00
Time
DNF-930 Gel: Separation of λ DNA/HindIII Marker (Promega®) with intact (D1)
and degraded (B1) genomic DNA.
A D VA N C E D A N A LY T I C A L
>
WWW.AATI-US.COM >DNA and RNA Quantity and Quality Measurements
>Restriction Digestion of DNA Fragments (CAPS Analysis)
>SSCP
>MLVA/MLPA
AdvanCE ™ FS96 dsDNA FR A G M E N T A N A LY Z E R
>A N A LY T I C A L S O F T W A R E
IMPROVE THROUGHPUT BY DECREASING ANALYSIS TIME
PRO Size™ data analysis software is designed to simplify
calibration and fragment identification, and automate
routine scoring and reporting of fragments.
Flexible Data Display
Data can be displayed in electropherogram or digital gel
views for any combination of rows, columns or individual
samples. Overlay random wells and adjust contrast, show/hide
markers, zoom/autoscale and copy to other applications.
Scoring and Output
Create, save and import multiple flag criteria for scoring
any data set. Results can be displayed and exported in
various formats including binary output. Reports can be
generated in Excel® or PDF format.
>T E C H N O LO G Y
AWARD-WINNING TECHNOLOGY * — UNIQUE, UNMATCHED CAPABILITIES
Advanced Analytical’s parallel capillary gel electrophoresis
separation technology dramatically improves laboratory
throughput and efficiency and reduces operational costs.
The AdvanCE™ FS96 system employs a powerful LED to
excite separated and intercalated dsDNA or RNA as it passes
through the capillary detection window, obviating the need
for labeled primers. A sensitive CCD detector is expertly
arranged to continuously collect the emitted signals. Proprietary signal processing provides superior dynamic range.
With simultaneous injection and separation of 96 individual
samples, the AdvanCE™ FS96 system is ideal for rapid, high
throughput analysis of dsDNA or RNA fragments. Vacuum
injection mode, a feature exclusive to the AdvanCE™ FS96
system, provides unique capabilities for high salt sample
matrices and more accurate quantification of nucleic acids.
A D VA N C E D A N A LY T I C A L
>
WWW.AATI-US.COM Capillaries
Sample or Buffer Tray
Fluorescence Excitation
Detection Window
Capillaries
CCD Detector
High Voltage
Power Supply
Outlet
*Best Scientific Poster at TIDES 2005 Conference sponsored
by BioProcess International.
*R&D Magazine’s Top 100 Technologies for 2001.
*R&D Magazine’s Most Promising New Technology Award 2001.
AdvanCE ™ FS96 dsDNA FRAG M E N T A N A LY Z E R
A d v a n C E ™ F S 9 6 d s D N A F R A G M E N T A N A LY Z E R
>K E Y F E AT U R E S / B E N E F I T S
>A 96-Capillary Array – Direct, parallel injection and
separation of an entire 96-well plate at once.
>Short Run Times – As little as 30 min for <1,000 bp.
With the fastest 96-well plate cycle time of any CE system
available, get more done in less time.
>High Sensitivity – Detection limits as low as 5 pg/μL without
desalting, allows direct injection of PCR and digest samples.
>High Separation Resolution – Resolve a wide range of
DNA/RNA fragments. Achieve as low as 2 bp resolution.
Custom gels can be developed.
>Multiple Injection Modes – Introduce samples by either
electrokinetic (voltage) or vacuum injection. Exclusive to
the AdvanCE™ system. Minimize impact from high salt or
variable salt matrices.
>W ide Dynamic Range – Covers 3 orders of magnitude.
Less optimization needed.
>Low Cost per Sample – Lower operational cost compared
to agarose/acrylamide gels and cost savings by eliminating
labeled primers.
>Robotics Interface Capability – Allows for unattended,
24 h operation with automated plate exchange.
>Variable Capillary Dimensions – Flexible capillary options
allow instrument to meet changing laboratory or project needs.
>User Friendly Software – Automate data scoring. Calculate
fragment size and concentration, display data in multiple
formats, create and save customized scoring parameters,
export and report results with ease.
>Real Time Viewing of Data – See results as they are being
generated, change conditions during a run if desired.
>A d v a n C E ™ F S 9 6 S Y S T E M T E C H N I C A L S P E C I F I C AT I O N S
Maximum Sample Throughput:
Maximum Unattended Sample Capacity:
Minimum Sample Volume:
Gel Sizing Ranges:
Resolution:
Sizing Accuracy:
Detection Sensitivity:
Light Source:
Detector:
Software:
Data Export Format:
Environmental Conditions:
Relative Humidity Range:
Electrical:
Instrument Dimensions:
Instrument Weight:
96 samples/30 min (20–1,000 bp fragment range)
192 samples; unlimited with robotic arm
20 μL for injection; as little as 1 μL sample required
10-500 bp; 50-2,000 bp; 500-12,000 bp; Genomic DNA
Gel dependent; to as low as 2 bp
Typically 5% or better
As low as 5 pg/μL in PCR matrix
1,000 mW LED, 470 nm excitation wavelength
High sensitivity CCD; 500-600 nm emission wavelength
AdvanCE™ instrument control, PRO Size™ data analysis
Microsoft® Excel® spreadsheet, binary output option
Indoor use, normal laboratory environment 20-23°C
< 80% (non-condensing)
100-200 VAC; 50-60 Hz; 15 A (Alternate configurations available)
Fully configured 99.1 cm H x 243.8 cm W x 76.2 cm D (39 x 96 x 30 in)
88.6 kg (195 lbs)
Advanced Analytical Technologies, Inc.
2711 South Loop Drive, Suite 4150
Ames, IA 50010 USA
Phone: +1-515-296-6600
Fax: +1-515-294-7141
E-mail: [email protected]
www.aati-us.com
Copyright © 2010 Advanced Analytical Technologies, Inc. All Rights Reserved. Reproduction, adaptation or translation without prior written permission is prohibited, except as allowed under copyright laws. AdvanCE™
is a trademark of Advanced Analytical Technologies, Inc.