Gain greater control in cell culture using
Cell-Ess® Serum Replacement
The ESSence of Cell Growth
Let it Grow
FBS contains over 300 factors that
can impact cell behavior in an
uncontrolled manner and no two lots
of FBS are the same.
Cell-Ess®
Gain control, performance,
convenience and a higher level of
certainty to your experiments.
Gain Control, Performance
and Convenience with
Cell-Ess® Serum Replacement
The Curse of Serum is that:
— Serum contains over 300 factors that change lot-to-lot.
That impacts cell growth and performance in an uncontrolled
manner, reducing experimental control
— To make serum usable, we manipulate it further through
gamma irradiation, heat inactivation or filtering, possibly
introducing more variation and cost
— T o manage the lack of control and performance inconsistency, we sacrifice convenience, time and materials when
screening multiple lots to secure a large inventory of a
“good” lot for future use
The Cure of Cell-Ess® brings:
Control – Defined components minimize variables. High lot
to lot consistency. No more lot testing
Performance – Different cell types perform well with Cell-Ess
and behave more consistently than with FBS
Convenience – Save time. No thawing, filtering or heat inactivation required. Purchase what you want when you need it
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Superior Control and
Performance with Cell-Ess®
Serum Replacement
1a. IL-2
Cell-Ess® supports cell functionality as measured by
activation and protein secretion, yet doesn’t contain
signaling factors that impact performance in…
…T-cell activation cultures
CD4 T cells were purified from mouse spleens then cultured,
activated with anti-CD3 and anti-CD28 in either FBS or Cell-Ess.
Cytokines were assayed 72 hours later.
Controlled results with Cell-Ess
While IL-2 response is similar in both groups (1a), IL-10 was
elevated only in FBS (1b) suggesting something in FBS, such as
TGF-ß, induced IL-10.
1b. IL-10
150
100
IL-10 pg/ml
IL-2 pg/ml
100
50
0
FBS
50
0
Cell-Ess®
2a. IL-10
… Macrophage activation cultures
FBS
Cell-Ess®
Controlled results with Cell-Ess
Potent cytokine response in LPS activated mouse macrophages
cultured in Cell-Ess. The IL-10 response between the 2 groups is
similar (2a), but IL-1ß is suppressed in FBS (2b), suggesting some-
2b. IL-1b
100
150
0
IL-1b pg/ml
IL-10 pg/ml
100
50
FBS
Cell-Ess
2
1.5
1
0.5
FBS
0
Cell-Ess®
thing in that lot, such as TGF-ß, suppressed the response.
FBS
Cell-Ess
®
3b. Death in activated CD4 T cells (LDH)
Relative Absorbance
Relative Absorbance
3a. Proliferation in activated CD4 T Cells
0
50
®
2.5
2
1.5
1
0.5
0
Peritoneal macrophages were isolated and plated. Purified
macrophages were then cultured and activated with LPS in
either FBS or Cell-Ess. Cytokines were assayed after 16 hours.
FBS
Cell-Ess®
… Cell proliferation you demand
Cell proliferation, shown here with T-cells, is equivalent to FBS
without the unknowns. Cell-Ess has robust proliferation with less
cell death.
Controlled results with Cell-Ess
CD4 T cells were purified from mouse spleens. Cytotoxicity was
assayed after 72 hours by LDH. There is less toxicity in T cells
cultured in Cell-Ess than in FBS. Cell-Ess supports robust proliferation (3a) and has less cell death than FBS (3b).
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The Curse of Serum
The Cure of Cell-Ess®
Serum contains unknown and variable levels of proteins, hormones,
Cell-Ess is completely synthetic, serum-free, animal component-free
cytokines and attachment factors, all of which affect cell behavior.
and hormone-free. No lot - to-lot variation re-establishes your control.
These variables are uncontrolled and can directly impact your
experimental results.
Serum may have contaminants such as viruses, prions, mycoplasma,
Cell-Ess is chemically defined with no animal components and
endotoxins, and other infective agents along with their byproducts.
is sterile-filtered.
Your cells can be compromised by using serum.
Serum often requires extra steps and treatments before use, including
Cell-Ess is never frozen and is ready to use without special treatments
thawing, heat inactivation, irradiating, filtering, charcoal stripping,
or handling. Start your cell culture faster and hassle free.
dialyzing, and other processes associated with purifying serum in one
way or another.
Each lot of FBS is unique; consequently, you spend time and effort
Cell-Ess is chemically defined and produced under cGMP conditions
testing multiple lots to find one that works best.
so each lot works just like the last and the next.
To ensure sufficient supply of a validated “good” serum lot, you
Buy Cell-Ess as you need it. Store it in your refrigerator, not your
are required to purchase enough to meet all project needs. This
freezer. Utilize your budget and storage space when you want to.
immediately ties up your budget and freezer space.
Serum-free media often contain growth factors and or transferrin
Cell-Ess has a dual-component design which provides full growth
placed into solution at the time of manufacture. The rapid
factor potency when you use it.
degradation of those factors begins at the time of solution leading to
reducing media performance over time.
Serum-free media are usually specialized for only one cell type. For
Cell-Ess is shown to work across numerous cell lines and with
several cell types, you will need several different serum-free media.
primary cells.
Serum-free media often fail to include lipids which may be required
Cell-Ess includes key lipids along with a unique delivery system to
for optimum cell growth and signaling.
make them available to cells.
Cell-Ess® Serum Replacement Products
and Specifications
Conduct cell culture with the cure of Cell-Ess
and none of serum’s curses
Example Cell Types Where Cell-Ess® is Used in Culture
Cell Lines
293 cells
K563 cells
PC-­6 cells
549 cells
MAC7 cells
SH-­SY5Y
CHO-­K1
MCF7
TK6 cells
HepG2
MDBK
Vero cells
mpkPPD cells
Part Code
Description
Size
HT-­29
25767-710-002
Cell-Ess Serum Replacement
100 ml
Primary Cells
25767-710-003
Cell-Ess® Serum Replacement
500 ml
25767-710-004
Cell-Ess® Serum Replacement
1000 ml
®
Format
Liquid
Storage
2 – 8° C in dark
Sterility
Mycoplasma, microbes, virus –
none detected
Adipocytes
HPBMCs
B cells
Macrophages
Bovine Luteal cells
Mast cells
Chondrocytes
Osteoblasts
Dendritic cells
Primary neurons
Endothelial cells
Prostate epithelial cells
Fibroblasts
Schwann cells
Hepatocytes
T cells
Stem Cells & Progenitor Cells
Canine MSCs
Mouse ES cells
Human MSCs
Mouse MSCs
Serum content
Serum free
Animal components
Animal free
Protein
< 2.0 mg/L
IgG
None
Save time. Be certain. Be in control.
Endotoxin
< 5.0 EU/mg
pH
6.9 –7.3
Order Cell-Ess®
Manufacturing compliance
cGMP, ISO 9001:2000
Hybrodoma Cell Lines
Hybridomas
Place your order at 1-866 413 6248
or order online at www.essentialpharma.com
All other inquiries 1-844-ESS-prod (1-844 377 7763)
or email us at [email protected]
www.cell-ess.com | www.essentialpharma.com
www.cell-ess.com | www.essentialpharma.com
Cell-Ess Serum Replacement
®
Essential Pharmaceuticals, LLC
100 Princeton South Corporate Center | Suite 140
Ewing, NJ 08628
1-844-Ess-Prod (1-844 377 7763)
CE-VIS-003