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B)11!W77!)86!M$&N"O%N.&.&!9,!2+:,29!9C+!<:311!9,!44QU//!)86!-,2!)<<3<9)84+>!! W'8'92*#$+$,'#$%'A*/.$"#;+$#"+.$' L)=N<:+43-34!38-,2@)93,8!,8!6+4,89)@38)93,8!@);!=+!384106+6!38!9C+!(2,9,4,1#(2,4+602+!<+493,8>! • • • a+)238*!:2,:+2!((QF!1)=,2)9,2;!D,2`!<02-)4+<!<C,016!=+!41+)8+6!)9!9C+!+86!,-!+)4C!D,2`!6);>!! ?3<:,<+!,-!4,89)@38)9+6!@)9+23)1<!38!)44,26)84+!D39C!C)Z)26,0<!D)<9+!63<:,<)1!*036+138+<! 2+-+2+84+6!=+1,D>! ?+4,89)@38)9+!)11!+Y03:@+89!=+-,2+!2+@,G38*!-2,@!)!6+<3*8)9+6!)2+)>! 7`'8'D#E#&%.01'V#1"*'A+1).1#2' L)=+1!a)<9+! L)=+1!)11!4,89)38+2<!D39C!9C+!1)=+1!:2,G36+6!)9A! !C99:A##+C<>=+2`+1+;>+60#C@#%R.N8+DNC)Z)26,0<ND)<9+N:2,*2)@NCD:>C9@1>! /++!9C+!QUi/!X)49!/C++9F!mU)Z)26,0<!a)<9+!K)8)*+@+89n!-,2!*+8+2)1!38<920493,8<!,8! :2,4+602+<!-,2!63<:,<38*!,-!C)Z)26,0<!D)<9+>! ?3<:,<+!,-!a)<9+! • • ?3<:,<+!,-!2+*01)21;!*+8+2)9+6!4C+@34)1!D)<9+!D39C38!"!@,89C<>! B)11!QUi/!D39C!Y0+<93,8<>! 77'8'!#3*">'A#"#'!:**"'a!A!b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ulfuric acid is a reagent used in organic reactions, and is a strong, corrosive acid and dehydrating agent. 0)6*,# 78928,,&289# :'8(&'*5+7;.2<=,8(# %%7#>,?,@#16),@#9*'A,5@# )*'(B289C# %&'),-.&,#0(,<5#68-#%&,)6.(2'85# <1mL up to 1L used (concentrated sulfuric acid & diluted solutions) All work using sulfuric acid must be performed in a ventilated fume hood. Eye protection: Wear tightfitting safety goggles or safety glasses with side shields. When working with concentrated sulfuric acid, it is important to make sure the work area is free of any bases which may react violently with the acid. Face protection: Wear a face shield when not protected by a lab hood sash. ! Gloves: Wear Butyl or Butyl/Viton gloves when handling sulfuric acid. Remove gloves as soon as contaminated. ! "#$$#%&$'! ()*+!"!,-!.! /01-0234!5436!/7(! When diluting sulfuric acid with water, the concentrated sulfuric acid should be added slowly to water to minimize the exothermic nature of the reaction and any dangers from splashing. ! Notes "#$$#%&$'! Clothing: Wear lab coat, full length pants or equivalent; and close-toed closed heeled shoes. Wear acid resistant apron when pouring concentrated sulfuric acid.! Any deviation from this SOP requires approval from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recautions for safe handling Do not allow water to get into the container because of violent reaction. Do not get in eyes, on skin, or on clothing. Keep container tightly closed. Use only with adequate ventilation. Do not breathe spray or mist. Do not use with metal spatula or other metal items. Inform laundry personnel of contaminant's hazards. When diluting sulfuric acid, always add acid (slowly) to cooled water. Never add water to acid. Conditions for safe storage Do not store near combustible materials. Keep container closed when not in use. Store in a cool, dry, and well-ventilated area, away from incompatible substances. Do not store near alkaline substances. Store protected from moisture. Ideally, sulfuric acid should be stored in isolation from all other chemicals in an approved acid or corrosives safety cabinet. Sulfuric Acid is incompatible with metals, oxidizing agents, reducing agents, bases, acrylonitrile, chlorates, finely powdered metals, nitrates, perchlorates, permanganates, epichlorohydrin, aniline, carbides, fulminates, picrates, organic materials, flammable liquids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